Three-dimensional conductive polycaprolactone/carbon nanotubes scaffolds for peripheral nerve regeneration
Source abstract
Abstract
Development of tissue-engineered alternatives to nerve autografts remains a serious challenge particularly considering the electroactive properties of native peripheral nerves. In this study, three-dimensional (3D) conductive nerve scaffolds composed of polycaprolactone (PCL) and carbon nanotubes (CNTs) were fabricated using an integration molding method. In vitro, the combination of PCL/CNTs scaffolds with electrical stimulation that mimic the endogenous electric fields could significantly promote Schwann cell proliferation and neurotrophic factor genes expression. In vivo, the effects of PCL/CNTs scaffolds on peripheral nerve regeneration were investigated using a 15-mm rat sciatic nerve defect model. Functional and histological tests indicated that PCL/CNTs scaffolds could effectively promote nerve regeneration and functional recovery. Therefore, PCL/CNTs scaffolds are promising candidates for peripheral nerve restoration.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nerve injury and regeneration · Neuroscience and Neural Engineering · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (45)
Show source-provided references
1. Jiang BG, Han N, Rao F et al (2017) Advance of peripheral nerve injury repair and reconstruction. Chin Med J (Engl) 130:2996–299810.4103/0366-6999.220299 ↗
2. Griffin JW, Hogan MV, Chhabra AB et al (2013) Peripheral nerve repair and reconstruction. J Bone Joint Surg Am 95:2144–215110.2106/JBJS.L.00704 ↗
3. Riccio M, Marchesini A, Pugliese P et al (2019) Nerve repair and regeneration: Biological tubulization limits and future perspectives. J Cell Physiol 234:3362–337510.1002/jcp.27299 ↗
4. Vijayavenkataraman S (2020) Nerve guide conduits for peripheral nerve injury repair: a review on design, materials and fabrication methods. Acta Biomater 106:54–6910.1016/j.actbio.2020.02.003 ↗
5. Gu X, Ding F, Williams DF (2014) Neural tissue engineering options for peripheral nerve regeneration. Biomaterials 35:6143–615610.1016/j.biomaterials.2014.04.064 ↗
6. Yi S, Xu L, Gu X (2019) Scaffolds for peripheral nerve repair and reconstruction. Exp Neurol 319:11276110.1016/j.expneurol.2018.05.016 ↗
7. Gordon T (2016) Electrical stimulation to enhance axon regeneration after peripheral nerve injuries in animal models and humans. Neurotherapeutics 13:295–31010.1007/s13311-015-0415-1 ↗
8. Wang L, Lu C, Yang S et al (2020) A fully biodegradable and self-electrified device for neuroregenerative medicine. Sci Adv 6:eabc668610.1126/sciadv.abc6686 ↗
9. Al-Majed AA, Neumann CM, Brushart TM et al (2000) Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration. J Neurosci 20:2602–260810.1523/JNEUROSCI.20-07-02602.2000 ↗
10. Elzinga K, Tyreman N, Ladak A et al (2015) Brief electrical stimulation improves nerve regeneration after delayed repair in Sprague Dawley rats. Exp Neurol 269:142–15310.1016/j.expneurol.2015.03.022 ↗
11. Guo B, Ma PX (2018) Conducting polymers for tissue engineering. Biomacromol 19:1764–178210.1021/acs.biomac.8b00276 ↗
12. Fraczek-Szczypta A (2014) Carbon nanomaterials for nerve tissue stimulation and regeneration. Mater Sci Eng C Mater Biol Appl 34:35–4910.1016/j.msec.2013.09.038 ↗
13. Carvalho CR, Silva-Correia J, Oliveira JM et al (2019) Nanotechnology in peripheral nerve repair and reconstruction. Adv Drug Deliv Rev 148:308–34310.1016/j.addr.2019.01.006 ↗
14. Manousiouthakis E, Park J, Hardy JG et al. (2021) Towards the translation of electroconductive organic materials for regeneration of neural tissues. Acta Biomater10.2139/ssrn.3802820 ↗
15. Fabbro A, Prato M, Ballerini L (2013) Carbon nanotubes in neuroregeneration and repair. Adv Drug Deliv Rev 65:2034–204410.1016/j.addr.2013.07.002 ↗
16. Hwang JY, Shin US, Jang WC et al (2013) Biofunctionalized carbon nanotubes in neural regeneration: a mini-review. Nanoscale 5:487–49710.1039/C2NR31581E ↗
17. Cellot G, Cilia E, Cipollone S et al (2009) Carbon nanotubes might improve neuronal performance by favouring electrical shortcuts. Nat Nanotechnol 4:126–13310.1038/nnano.2008.374 ↗
18. Hu H, Ni Y, Montana V et al (2004) Chemically functionalized carbon nanotubes as substrates for neuronal growth. Nano Lett 4:507–51110.1021/nl035193d ↗
19. Mattson MP, Haddon RC, Rao AM (2000) Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth. J Mol Neurosci 14:175–18210.1385/JMN:14:3:175 ↗
20. Lovat V, Pantarotto D, Lagostena L et al (2005) Carbon nanotube substrates boost neuronal electrical signaling. Nano Lett 5:1107–111010.1021/nl050637m ↗
21. Wu S, Qi Y, Shi W et al. (2020) Electrospun conductive nanofiber yarns for accelerating mesenchymal stem cells differentiation and maturation into Schwann cell-like cells under a combination of electrical stimulation and chemical induction. Acta Biomater10.2139/ssrn.3674052 ↗
22. Galvan-Garcia P, Keefer EW, Yang F et al (2007) Robust cell migration and neuronal growth on pristine carbon nanotube sheets and yarns. J Biomater Sci Polym Ed 18:1245–126110.1163/156856207782177891 ↗
23. Wang L, Wu Y, Hu T et al (2019) Aligned conductive core-shell biomimetic scaffolds based on nanofiber yarns/hydrogel for enhanced 3D neurite outgrowth alignment and elongation. Acta Biomater 96:175–18710.1016/j.actbio.2019.06.035 ↗
24. Su WT, Shih YA (2015) Nanofiber containing carbon nanotubes enhanced PC12 cell proliferation and neuritogenesis by electrical stimulation. Biomed Mater Eng 26(Suppl 1):S189–S195
25. Gonçalves C, Ribeiro J, Pereira T et al (2016) Preparation and characterization of electrical conductive PVA based materials for peripheral nerve tube-guides. J Biomed Mater Res A 104:1981–198710.1002/jbm.a.35730 ↗
26. Dash TK, Konkimalla VB (2012) Poly-є-caprolactone based formulations for drug delivery and tissue engineering: a review. J Control Release 158:15–3310.1016/j.jconrel.2011.09.064 ↗
27. Zhu L, Wang K, Ma T et al (2017) Noncovalent bonding of RGD and YIGSR to an electrospun poly(ε-caprolactone) conduit through peptide self-assembly to synergistically promote sciatic nerve regeneration in rats. Adv Healthc Mater 6:160086010.1002/adhm.201600860 ↗
28. Reid AJ, de Luca AC, Faroni A et al (2013) Long term peripheral nerve regeneration using a novel PCL nerve conduit. Neurosci Lett 544:125–13010.1016/j.neulet.2013.04.001 ↗
29. Jiang X, Mi R, Hoke A et al (2014) Nanofibrous nerve conduit-enhanced peripheral nerve regeneration. J Tissue Eng Regen Med 8:377–38510.1002/term.1531 ↗
30. Wang J, Cheng Y, Chen L et al (2019) In vitro and in vivo studies of electroactive reduced graphene oxide-modified nanofiber scaffolds for peripheral nerve regeneration. Acta Biomater 84:98–11310.1016/j.actbio.2018.11.032 ↗
31. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–40810.1006/meth.2001.1262 ↗
32. de Medinaceli L, Freed WJ, Wyatt RJ (1982) An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol 77:634–64310.1016/0014-4886(82)90234-5 ↗
33. Jessen KR, Mirsky R, Lloyd AC (2015) Schwann cells: development and role in nerve repair. Cold Spring Harb Perspect Biol 7:a02048710.1101/cshperspect.a020487 ↗
34. Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M et al (2009) Electrical stimulation of nerve cells using conductive nanofibrous scaffolds for nerve tissue engineering. Tissue Eng Part A 15:3605–361910.1089/ten.tea.2008.0689 ↗
35. Zhang J, Qiu K, Sun B et al (2014) The aligned core-sheath nanofibers with electrical conductivity for neural tissue engineering. J Mater Chem B 2:7945–795410.1039/C4TB01185F ↗
36. Frostick SP, Yin Q, Kemp GJ (1998) Schwann cells, neurotrophic factors, and peripheral nerve regeneration. Microsurgery 18:397–40510.1002/(SICI)1098-2752(1998)18:7<397::AID-MICR2>3.0.CO;2-F ↗
37. Hoyng SA, De Winter F, Gnavi S et al (2014) A comparative morphological, electrophysiological and functional analysis of axon regeneration through peripheral nerve autografts genetically modified to overexpress BDNF, CNTF, GDNF, NGF, NT3 or VEGF. Exp Neurol 261:578–59310.1016/j.expneurol.2014.08.002 ↗
38. Liu S, Liu Y, Zhou L et al (2021) XT-type DNA hydrogels loaded with VEGF and NGF promote peripheral nerve regeneration via a biphasic release profile. Biomater Sci 9:8221–823410.1039/D1BM01377G ↗
39. Wang B, Lu CF, Liu ZY et al (2022) Chitin scaffold combined with autologous small nerve repairs sciatic nerve defects. Neural Regen Res 17:1106–111410.4103/1673-5374.324859 ↗
40. Yang S, Zhu J, Lu C et al (2022) Aligned fibrin/functionalized self-assembling peptide interpenetrating nanofiber hydrogel presenting multi-cues promotes peripheral nerve functional recovery. Bioact Mater 8:529–54410.1016/j.bioactmat.2021.05.056 ↗
41. Mata M, Alessi D, Fink DJ (1990) S100 is preferentially distributed in myelin-forming Schwann cells. J Neurocytol 19:432–44210.1007/BF01188409 ↗
42. Posmantur R, Hayes RL, Dixon CE et al (1994) Neurofilament 68 and neurofilament 200 protein levels decrease after traumatic brain injury. J Neurotrauma 11:533–54510.1089/neu.1994.11.533 ↗
43. Saffari TM, Bedar M, Hundepool CA et al (2020) The role of vascularization in nerve regeneration of nerve graft. Neural Regen Res 15:1573–157910.4103/1673-5374.276327 ↗
44. Tondnevis F, Keshvari H, Mohandesi JA (2020) Fabrication, characterization, and in vitro evaluation of electrospun polyurethane-gelatin-carbon nanotube scaffolds for cardiovascular tissue engineering applications. J Biomed Mater Res B Appl Biomater 108:2276–229310.1002/jbm.b.34564 ↗
45. Jiang C, Wang K, Liu Y et al (2021) Using wet electrospun PCL/Gelatin/CNT yarns to fabricate textile-based scaffolds for vascular tissue engineering. ACS Biomater Sci Eng 7:2627–263710.1021/acsbiomaterials.1c00097 ↗