Surface modifications to boost sensitivities of electrochemical biosensors using gold nanoparticles/silicon nanowires and response surface methodology approach
Source abstract
Abstract
This work describes fabrication of a DNA electrochemical sensor utilized of gold nanoparticles/silicon nanowires/indium tin oxide (AuNPs/SiNWs/ITO) as a modified substrate for detection of dengue virus DNA oligomers using methylene blue (MB) as a redox indicator. The response surface methodology (RSM) was applied as one of the advanced optimization methods for fabrication of SiNWs/AuNPs/ITO electrode and immobilization of DNA probes to enhance the sensitivity of DNA detection. Several factors were successfully optimized using RSM, including volume of SiNWs, concentration of dithiopropionic acid (DTPA), volume of AuNPs, DNA probe concentration, and DNA probe immobilization time. RSM approach shows that AuNPs and DNA probe concentration were the prominent factors affecting on the MB current signal and immobilization of DNA probe on AuNPs/SiNWs surface. This new developed sensor was able to discriminate complementary target sequences, noncomplementary and single-base mismatch sequences, for DNA dengue virus detection.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · Mosquito-borne diseases and control · Biosensors and Analytical Detection
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (60)
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1. Cui H-F, Cheng L, Zhang J, Liu R, Zhang C, Fan H (2014) An electrochemical DNA sensor for sequence-specific DNA recognization in a homogeneous solution. Biosens Bioelectron 56:124–12810.1016/j.bios.2013.12.027 ↗
2. Li F, Chen W, Zhang S (2008) Development of DNA electrochemical biosensor based on covalent immobilization of probe DNA by direct coupling of sol–gel and self-assembly technologies. Biosens Bioelectron 24(4):781–78610.1016/j.bios.2008.06.052 ↗
3. Zhang C, Xu S, Zhang X, Huang D, Li R, Zhao S, Wang B (2014) Electrochemical detection of specific DNA sequences related to bladder cancer on CdTe quantum dots modified glassy carbon electrode. J Electroanal Chem 735:115–12210.1016/j.jelechem.2014.09.014 ↗
4. Sassolas A, Leca-Bouvier BD, Blum LJ (2008) DNA biosensors and microarrays. Chem Rev 108(1):109–13910.1021/cr0684467 ↗
5. Chowdhury AD, Gangopadhyay R, De A (2014) Highly sensitive electrochemical biosensor for glucose, DNA and protein using gold-polyaniline nanocomposites as a common matrix. Sens Actuators B Chem 190:348–35610.1016/j.snb.2013.08.071 ↗
6. Rashid JIA, Yusof NA, Abdullah J, Hashim U, Hajian R (2014) The utilization of SiNW/AuNP-modified indium tin oxide (ITO) in fabrication of electrochemical DNA sensor. Mater Sci Eng C 45:270–27610.1016/j.msec.2014.09.010 ↗
7. Rashid J, Yusof N, Abdullah J, Hashim U, Hajian R (2015) A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection. IEEE Sens J. doi: 10.1109/JSEN.2015.2417911
8. Farjami E, Clima L, Gothelf K, Ferapontova EE (2010) DNA interactions with a methylene blue redox indicator depend on the DNA length and are sequence specific. Analyst 135:1443–1448. doi: 10.1039/c0an00049c10.1039/c0an00049c ↗
9. Velusamy V, Arshak K, Yang CF, Yu L, Korostynska O, Adley C (2011) Comparison between DNA immobilization techniques on a redox polymer matrix. Am J Anal Chem 2:392–400. doi: 10.4236/ajac.2011.2304810.4236/ajac.2011.23048 ↗
10. Qi X, Gao H, Zhang Y, Wang X, Chen Y, Sun W (2012) Electrochemical DNA biosensor with chitosan-Co3O4 nanorod-graphene composite for the sensitive detection of Staphylococcus aureus nuc gene sequence. Bioelectrochemistry 88:42–47. doi: 10.1016/j.bioelechem.2012.05.00710.1016/j.bioelechem.2012.05.007 ↗
11. Primo EN, Belén Oviedo M, Sánchez CG, Rubianes MD, Rivas GA (2014) Bioelectrochemical sensing of promethazine with bamboo-type multiwalled carbon nanotubes dispersed in calf-thymus double stranded DNA. Bioelectrochemistry. doi: 10.1016/j.bioelechem.2014.05.002
12. Shi A, Wang J, Han X, Fang X, Zhang Y (2014) A sensitive electrochemical DNA biosensor based on gold nanomaterial and graphene amplified signal. Sens Actuators B Chem 200:206–212. doi: 10.1016/j.snb.2014.04.02410.1016/j.snb.2014.04.024 ↗
13. Yang L, Lin H, Zhang Z, Cheng L, Ye S, Shao M (2013) Gas sensing of tellurium-modified silicon nanowires to ammonia and propylamine. Sens Actuators B Chem 177:260–26410.1016/j.snb.2012.10.136 ↗
14. Oh JY, Jang H-J, Cho W-J, Islam MS (2012) Highly sensitive electrolyte-insulator-semiconductor pH sensors enabled by silicon nanowires with Al2O3SiO2 sensing membrane. Sens Actuators B Chem 171:238–243
15. Rashid JIA, Abdullah J, Yusof NA, Hajian R (2013) The development of silicon nanowire as sensing material and its applications. J Nanomater 2013:1–16. doi: 10.1155/2013/328093
16. Yan Q, Wang Z, Zhang J, Peng H, Chen X, Hou H, Liu C (2012) Nickel hydroxide modified silicon nanowires electrode for hydrogen peroxide sensor applications. Electrochim Acta 61:148–153. doi: 10.1016/j.electacta.2011.11.09810.1016/j.electacta.2011.11.098 ↗
17. Su S, He Y, Zhang M, Yang K, Song S, Zhang X, Fan C, Lee S-T (2008) High-sensitivity pesticide detection via silicon nanowires-supported acetylcholinesterase-based electrochemical sensors. Appl Phys Lett 93(2):023113. doi: 10.1063/1.295982710.1063/1.2959827 ↗
18. Kwon DH, An HH, Kim H-S, Lee JH, Suh SH, Kim YH, Yoon CS (2011) Electrochemical albumin sensing based on silicon nanowires modified by gold nanoparticles. Appl Surf Sci 257(10):4650–4654. doi: 10.1016/j.apsusc.2010.12.10910.1016/j.apsusc.2010.12.109 ↗
19. Chen W, Yao H, Tzang CH, Zhu J, Yang M, Lee S-T (2006) Silicon nanowires for high-sensitivity glucose detection. Appl Phys Lett 88(21):213104. doi: 10.1063/1.220610210.1063/1.2206102 ↗
20. Su S, Wei X, Guo Y, Zhong Y, Su Y, Huang Q, Fan C, He Y (2013) A Silicon nanowire-based electrochemical sensor with high sensitivity and electrocatalytic activity. Part Part Syst Charact 30(4):326–331. doi: 10.1002/ppsc.20120007610.1002/ppsc.201200076 ↗
21. Tao B, Zhang J, Hui S, Wan L (2009) An amperometric ethanol sensor based on a Pd–Ni/SiNWs electrode. Sens Actuators B Chem 142(1):298–303. doi: 10.1016/j.snb.2009.08.00410.1016/j.snb.2009.08.004 ↗
22. Yang K, Wang H, Zou K, Zhang X (2006) Gold nanoparticle modified silicon nanowires as biosensors. Nanotechnology 17(11):S276–S279. doi: 10.1088/0957-4484/17/11/s0810.1088/0957-4484/17/11/S08 ↗
23. Myers RH, Anderson-Cook CM (2009) Response surface methodology: process and product optimization using designed experiments, vol 705. Wiley, New York
24. Rashid A, Izuan J, Samat N, Wan Yusoff WM (2011) Optimization of temperature, moisture content and inoculum size in solid state fermentation to enhance mannanase production by Aspergillus terreus SUK-1 using RSM. Pak J Biol Sci 14(9):533–53910.3923/pjbs.2011.533.539 ↗
25. Rashid JIA, Samat N, Yusoff WMW (2012) Screening and optimization of medium composition for mannanase production by Aspergillus terreus SUK-1 in solid state fermentation using statistical experimental methods. Res J Microbiol 7(5):24210.3923/jm.2012.242.255 ↗
26. Alizadeh T, Zare M (2009) Enhancement of sensitivity of molecularly imprinted polymer based parathion voltammetric sensor by using experimental design technique. Anal Bioanal Electrochem 2009(3):169–187
27. Uliana CV, Riccardi CS, Tognolli JO, Yamanaka H (2008) Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs. J Braz Chem Soc 19(4):782–78710.1590/S0103-50532008000400024 ↗
28. Mirmoghtadaie L, Ensafi AA, Kadivar M, Norouzi P (2013) Highly selective electrochemical biosensor for the determination of folic acid based on DNA modified-pencil graphite electrode using response surface methodology. Mater Sci Eng C 33(3):1753–175810.1016/j.msec.2012.12.090 ↗
29. Urkut Z, Kara P, Goksungur Y, Ozsoz M (2011) Response surface methodology for optimization of food borne pathogen detection in real samples based on label free electrochemical nucleic acid biosensors. Electroanalysis 23(11):2668–2676. doi: 10.1002/elan.20110031010.1002/elan.201100310 ↗
30. Nasir SH, Hadi N (2008) Gold nanoparticles embedded on the surface of polyvinyl alcohol layer. J Fundam Sci 4:245–252
31. Yguerabide J, Yguerabide EE (1998) Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications: I. Theory. Anal Biochem 262(2):137–15610.1006/abio.1998.2759 ↗
32. Luo C, Tang H, Cheng W, Yan L, Zhang D, Ju H, Ding S (2013) A sensitive electrochemical DNA biosensor for specific detection of Enterobacteriaceae bacteria by Exonuclease III-assisted signal amplification. Biosens Bioelectron 48:132–137. doi: 10.1016/j.bios.2013.03.08410.1016/j.bios.2013.03.084 ↗
33. Jain R, Sharma S (2012) Glassy carbon electrode modified with multi-walled carbon nanotubes sensor for the quantification of antihistamine drug pheniramine in solubilized systems. J Pharm Anal 2(1):56–6110.1016/j.jpha.2011.09.013 ↗
34. Aydogdu G, Zeybek DK, Pekyardimci S, Kiliç E (2013) A novel amperometric biosensor based on ZnO nanoparticles-modified carbon paste electrode for determination of glucose in human serum. Artif Cells Nanomed Biotechnol 41(5):332–33810.3109/21691401.2012.744994 ↗
35. S-f Liu, Y-f Li, J-r Li, Jiang L (2005) Enhancement of DNA immobilization and hybridization on gold electrode modified by nanogold aggregates. Biosens Bioelectron 21(5):789–79510.1016/j.bios.2005.02.001 ↗
36. Ilkhani H, Arvand M, Ganjali MR, Marrazza G, Mascini M (2013) Nanostructured screen printed graphite electrode for the development of a novel electrochemical genosensor. Electroanalysis 25(2):507–51410.1002/elan.201200556 ↗
37. Chen L, Zhang L, Qiu T, Cao W (2011) Chemiluminescent detection of DNA hybridization based on signal DNA probe modified with gold and cobalt nanoparticles. Int J Electrochem Sci 6:5325–533610.1016/S1452-3981(23)18410-0 ↗
38. Liu S, Liu J, Wang L, Zhao F (2010) Development of electrochemical DNA biosensor based on gold nanoparticle modified electrode by electroless deposition. Bioelectrochemistry 79(1):37–42. doi: 10.1016/j.bioelechem.2009.10.00510.1016/j.bioelechem.2009.10.005 ↗
39. Hezard T, Fajerwerg K, Evrard D, Collière V, Behra P, Gros P (2012) Gold nanoparticles electrodeposited on glassy carbon using cyclic voltammetry: application to Hg(II) trace analysis. J Electroanal Chem 664:46–5210.1016/j.jelechem.2011.10.014 ↗
40. Bettazzi F, Lucarelli F, Palchetti I, Berti F, Marrazza G, Mascini M (2008) Disposable electrochemical DNA-array for PCR amplified detection of hazelnut allergens in foodstuffs. Anal Chim Acta 614(1):93–10210.1016/j.aca.2008.03.027 ↗
41. Pournaghi-Azar M, Hejazi M, Alipour E (2006) Developing an electrochemical deoxyribonucleic acid (DNA) biosensor on the basis of human interleukine-2 gene using an electroactive label. Anal Chim Acta 570(2):144–15010.1016/j.aca.2006.04.067 ↗
42. Loaiza ÓA, Campuzano S, López-Berlanga M, Pedrero M, Pingarrón JM (2005) Development of a DNA sensor based on alkanethiol self-assembled monolayer-modified electrodes. Sensors 5(6):344–36310.3390/s5060344 ↗
43. Alegret S, Merkoçi A (2007) Electrochemical sensor analysis, vol 49. Elsevier, Amsterdam
44. Souza E, Nascimento G, Santana N, Danielly F, Lima M, Natividade E, Martins D, Lima-Filho J (2011) Label-free electrochemical detection of the specific oligonucleotide sequence of dengue virus type 1 on pencil graphite electrodes. Sensors 11:5616–562910.3390/s110605616 ↗
45. Oliveira N, Souza E, Ferreira D, Zanforlin D, Bezerra W, Borba MA, Arruda M, Lopes K, Nascimento G, Martins D (2015) A sensitive and selective label-free electrochemical DNA biosensor for the detection of specific dengue virus serotype 3 sequences. Sensors 15(7):15562–1557710.3390/s150715562 ↗
46. Tedjo A, Ibrahim AS, Kusmardi (2010) Photoelectrochemical detection of dengue-related oligonucleotide sequence using anthocyanin as a intercalating agent and electrochromic material. Makara Seri Kesehatan 14(2):70–74
47. Ribeiro Teles FR, França dos Prazeres DM, de Lima-Filho JL (2007) Electrochemical detection of a dengue-related oligonucleotide sequence using ferrocenium as a hybridization indicator. Sensors 7(11):2510–251810.3390/s7112510 ↗
48. Iyer MA, Oza G, Velumani S, Maldonado A, Romero J, Muñoz MdL, Sridharan M, Asomoza R, Yi J (2014) Scanning fluorescence-based ultrasensitive detection of dengue viral DNA on ZnO thin films. Sens Actuators B Chem 202:1338–134810.1016/j.snb.2014.06.005 ↗
49. Deng J, Toh CS (2013) Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus. Sensors 13:7774–7785. doi: 10.3390/s13060777410.3390/s130607774 ↗
50. Huang S, Li C, Lin B, Qin J (2010) Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization. Lab Chip 10:2925–2931. doi: 10.1039/c005227b10.1039/c005227b ↗
51. Zhang G-J, Zhang L, Huang MJ, Luo ZHH, Tay GKI, Lim E-JA, Kang TG, Chen Y (2010) Silicon nanowire biosensor for highly sensitive and rapid detection of Dengue virus. Sens Actuators B Chem 146(1):138–14410.1016/j.snb.2010.02.021 ↗
52. Rai V, Hapuarachchi HC, Ng LC, Soh SH, Leo YS, Toh C-S (2012) Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor. PLoS ONE 7(8):e4234610.1371/journal.pone.0042346 ↗
53. Senapati S, Slouka Z, Shah SS, Behura SK, Shi Z, Stack MS, Severson DW, Chang H-C (2014) An ion-exchange nanomembrane sensor for detection of nucleic acids using a surface charge inversion phenomenon. Biosens Bioelectron 60:92–10010.1016/j.bios.2014.04.008 ↗
54. Zaytseva NV, Montagna RA, Baeumner AJ (2005) Microfluidic biosensor for the serotype-specific detection of dengue virus RNA. Anal Chem 77(23):7520–752710.1021/ac0509206 ↗
55. Díaz-Badillo A, Muñoz ML, Ramirez GL, Altuzar V, Burgueño J, Alvarez JGM, Muñoz JPM, Cisneros A, Espinosaand JN, Sinencio FS (2014) A DNA microarray-based assay to detect dual infection with two dengue virus serotypes. Sensors 14:7580–7601. doi: 10.3390/s14050758010.3390/s140507580 ↗
56. Abdul Rahman S, Saadun R, Azmi NE, Ariffin N, Abdullah J, Yusof NA, Sidek H, Hajian R (2014) Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles. J Nanomater 2014:1–5. doi: 10.1155/2014/83928610.1155/2014/839286 ↗
57. Nascimento HP, Oliveira MD, de Melo CP, Silva GJ, Cordeiro MT, Andrade CA (2011) An impedimetric biosensor for detection of dengue serotype at picomolar concentration based on gold nanoparticles-polyaniline hybrid composites. Colloids Surf B 86(2):414–41910.1016/j.colsurfb.2011.04.028 ↗
58. Lee Yu HL, Montesa CM, Rojas NSL, Enriquez EP (2012) Nucleic-acid based lateral flow strip biosensor via competitive binding for possible dengue detection. J Biosens Biolectron 3(5):128. doi: 10.4172/2155-6210.1000128
59. Chen S-H, Chuang Y-C, Lu Y-C, Lin H-C, Yang Y-L, Lin C-S (2009) A method of layer-by-layer gold nanoparticle hybridization in a quartz crystal microbalance DNA sensing system used to detect dengue virus. Nanotechnology 20(21):21550110.1088/0957-4484/20/21/215501 ↗
60. Ngo HT, Wang HS, Fales A, Nicholson B, Woods CW, Vo-Dinh T (2014) DNA bioassay-on-chip using SERS detection for dengue diagnosis. Analyst 139:5655–5659. doi: 10.1039/c4an01077a10.1039/C4AN01077A ↗