journal article·2022-05

Tuning the antimicrobial efficacy of nano-Ca(OH)2 against E. coli using molarity

Harish, Pushpendra Kumar, Sapna Kumari, Mousumi Debnath, Amena Salim, Rahul Singhal, Rajendra P. Joshi, Anoop Kumar Mukhopadhyay

Journal of Materials Science· Volume 57· Issue 17
Publisher page

Abstract

The present work reports on tuning the antimicrobial efficacy of nano-Ca(OH)2 against E. coli by appropriately tuning the molarity of the reactants. Thus, the phase pure nano-Ca(OH)2 powders are developed by an inexpensive chemical precipitation technique using equimolar concentrations (e.g., 0.4, 0.6, 0.8, and 1 M) of [Ca(NO3)2·4H2O] and NaOH solutions. The characterizations by the XRD, FESEM, TEM, FTIR, DTA, TGA, UV–Vis spectroscopy, and agar plate well diffusion methods show that the higher the molarity of reactants, the higher the nanocrystallite size, the lower the optical band gap energy and the higher the (%) increase in inhibition zone diameters (Diz) for exposure periods in the range of 6–48 h. These results are discussed in terms of relative variations in the microstructure, lattice strain, thermal stability, optical band gap energy, defect structure, and the amount of (OH−) ions. Further, the possible mechanisms of antimicrobial behavior are suggested. Finally, the implications of these results in terms of microstructurally tuned nano-Ca(OH)2 materials development for prospective futuristic applications are highlighted.
Source: expertdata · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Bone Tissue Engineering Materials · Endodontics and Root Canal Treatments · Calcium Carbonate Crystallization and Inhibition

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

References (63)

Show source-provided references
1. Komabayashi T, Ahn C, Spears R, Zhu Q (2014) Comparison of particle morphology between commercial- and research-grade calcium hydroxide in endodontics. J Oral Sci 56:195–199. https://doi.org/10.2334/josnusd.56.19510.2334/josnusd.56.195 ↗
2. Bahman S, Sara G, Somayeh H, Parvin T, Kalhori KAM, Mona S, Reza F (2020) Combined effects of calcium hydroxide and photobiomodulation therapy on apexogenesis of immature permanent teeth in dogs. J Photochem Photobiol B 207:111867. https://doi.org/10.1016/j.jphotobiol.2020.11186710.1016/j.jphotobiol.2020.111867 ↗
3. Elchaghaby MA, Moheb DM, El Shahawy OI, Abd Alsamad AM, Rashed MAM (2020) Clinical and radiographic evaluation of indirect pulp treatment of young permanent molars using photo-activated oral disinfection versus calcium hydroxide: a randomized controlled pilot trial. BDJ Open 6:4–4. https://doi.org/10.1038/s41405-020-0030-z10.1038/s41405-020-0030-z ↗
4. Thawre S, Joshi R, Bhardwaj SB, Bhushan J (2020) Comparison of the antibacterial efficacy of tea, tree oil, nisin and calcium hydroxide against Enterococcus faecalis. Mater Today Proc 28:1477–1480. https://doi.org/10.1016/j.matpr.2020.04.82410.1016/j.matpr.2020.04.824 ↗
5. Afkhami F, Rostami G, Batebi S, Bahador A (2021) Residual antibacterial effects of a mixture of silver nanoparticles/calcium hydroxide and other root canal medicaments against Enterococcus faecalis. J Dent Sci. https://doi.org/10.1016/j.jds.2021.11.01310.1016/j.jds.2021.11.013 ↗
6. Yamanaka S, Hirano S, Uwai K, Tokuraku K (2020) Design of calcium hydroxide–based granules for livestock sanitation. Case Stud Chem Environ Eng 2:100005. https://doi.org/10.1016/j.cscee.2020.10000510.1016/j.cscee.2020.100005 ↗
7. Natali I, Tempesti P, Carretti E, Potenza M, Sansoni S, Baglioni P, Dei L (2014) Aragonite crystals grown on bones by reaction of CO2 with nanostructured Ca(OH)2 in the presence of collagen. Implic Archaeol Paleont Langmuir 30:660–668. https://doi.org/10.1021/la404085v10.1021/la404085v ↗
8. Lee H-G, Cho CH, Kim HK, Yoo S (2020) Improved physical and mechanical properties of food packaging films containing calcium hydroxide as a CO2 adsorbent by stearic acid addition. Food Packag Shelf Life 26:100558. https://doi.org/10.1016/j.fpsl.2020.10055810.1016/j.fpsl.2020.100558 ↗
9. Gollsch M, Afflerbach S, Angadi BV, Linder M (2020) Investigation of calcium hydroxide powder for thermochemical storage modified with nanostructured flow agents. Sol Energy 201:810–818. https://doi.org/10.1016/j.solener.2020.03.03310.1016/j.solener.2020.03.033 ↗
10. Funayama S, Takasu H, Kim ST, Kato Y (2020) Thermochemical storage performance of a packed bed of calcium hydroxide composite with a silicon-based ceramic honeycomb support. Energy 201:117673. https://doi.org/10.1016/j.energy.2020.11767310.1016/j.energy.2020.117673 ↗
11. Lei J, Law WW, Yang E-H (2021) Effect of calcium hydroxide on the alkali-silica reaction of alkali-activated slag mortars activated by sodium hydroxide. Constr Build Mater 272:121868. https://doi.org/10.1016/j.conbuildmat.2020.12186810.1016/j.conbuildmat.2020.121868 ↗
12. Liang Y (2020) Mechanical and fracture properties of calcium silicate hydrate and calcium hydroxide composite from reactive molecular dynamics simulations. Chem Phys Lett 761:138117. https://doi.org/10.1016/j.cplett.2020.13811710.1016/j.cplett.2020.138117 ↗
13. Swain SK, Bhattacharyya S, Sarkar D (2015) Fabrication of porous hydroxyapatite scaffold via polyethylene glycol-polyvinyl alcohol hydrogel state. Mater Res Bull 64:257–261. https://doi.org/10.1016/j.materresbull.2014.12.07210.1016/j.materresbull.2014.12.072 ↗
14. Granizo ML, Alonso S, Blanco-Varela MT, Palomo A (2002) Alkaline activation of metakaolin: effect of calcium hydroxide in the products of reaction. J Am Ceram Soc 85:225–231. https://doi.org/10.1111/j.1151-2916.2002.tb00070.x10.1111/j.1151-2916.2002.tb00070.x ↗
15. Rodriguez-Navarro C, Suzuki A, Ruiz-Agudo E (2013) Alcohol dispersions of calcium hydroxide nanoparticles for stone conservation. Langmuir 29:11457–11470. https://doi.org/10.1021/la401772810.1021/la4017728 ↗
16. López-Arce P, Gomez-Villalba LS, Pinho L, Fernández-Valle ME, de Buergo MÁ, Fort R (2010) Influence of porosity and relative humidity on consolidation of dolostone with calcium hydroxide nanoparticles: effectiveness assessment with non-destructive techniques. Mater Charact 61:168–184. https://doi.org/10.1016/j.matchar.2009.11.00710.1016/j.matchar.2009.11.007 ↗
17. Dei L, Salvadori B (2006) Nanotechnology in cultural heritage conservation: nanometric slaked lime saves architectonic and artistic surfaces from decay. J Cult Herit 7:110–115. https://doi.org/10.1016/j.culher.2006.02.00110.1016/j.culher.2006.02.001 ↗
18. Ogata F, Kagiyama Y, Saenjum C, Nakamura T, Kawasaki N (2020) Performance of poly-γ-glutamic acid–calcium hydroxide treatment for phosphate removal and applicability of the resulting flocculant as a phosphate-based fertilizer. Bioresour Technol Rep 11:100464. https://doi.org/10.1016/j.biteb.2020.10046410.1016/j.biteb.2020.100464 ↗
19. Antony D, Yadav R, Kalimuthu R, Kumuthan MS (2022) Phyto-complexation of galactomannan-stabilized calcium hydroxide and selenium-calcium hydroxide nanocomposite to enhance the seed-priming effect in Vigna radiata. Int J Biol Macromol 194:933–944. https://doi.org/10.1016/j.ijbiomac.2021.11.14810.1016/j.ijbiomac.2021.11.148 ↗
20. Samanta A, Podder S, Ghosh CK, Bhattacharya M, Ghosh J, Mallik AK, Dey A, Mukhopadhyay AK (2017) ROS mediated high anti-bacterial efficacy of strain tolerant layered phase pure nano-calcium hydroxide. J Mech Behav Biomed Mater 72:110–128. https://doi.org/10.1016/j.jmbbm.2017.04.00410.1016/j.jmbbm.2017.04.004 ↗
21. Khachani M, El Hamidi A, Halim M, Arsalane S (2014) Non-isothermal kinetic and thermodynamic studies of the dehydroxylation process of synthetic calcium hydroxide Ca(OH)2. J Mater Environ Sci 5:615–624. https://doi.org/10.1016/j.jmbbm.2017.04.00410.1016/j.jmbbm.2017.04.004 ↗
22. Schaube F, Koch L, Wörner A, Müller-Steinhagen H (2012) A thermodynamic and kinetic study of the de- and rehydration of Ca(OH)2 at high H2O partial pressures for thermo-chemical heat storage. Thermochim Acta 538:9–20. https://doi.org/10.1016/j.tca.2012.03.00310.1016/j.tca.2012.03.003 ↗
23. Dutta S, Shirai T (1974) Kinetics of drying and decomposition of calcium hydroxide. Chem Eng Sci 29:2000–2003. https://doi.org/10.1016/0009-2509(74)85021-910.1016/0009-2509(74)85021-9 ↗
24. Beaudoin JJ, Sato T, Tumidajski PJ (2006) The Thermal decomposition of Ca(OH)2 polymorphs. In: 2nd International Symposium on Advances in Concrete Through Science and Engineering, RILEM, Québec City, Canada. pp 1–11. https://nrc-publications.canada.ca/eng/view/accepted/?id=bedec8af-029a-4fe9-96e3-55790cfbb64810.1617/2351580028.001 ↗
25. Bayliss P (1964) Effect of particle size on differential thermal analysis. Nature 201:1019–1019. https://doi.org/10.1038/2011019a010.1038/2011019a0 ↗
26. Van Der Marel HW (1956) Quantitative differential thermal analyses of clay and other minerals. Am Mineral 41:222–244
27. Pishtshev A, Karazhanov S, Klopov M (2014) Materials properties of magnesium and calcium hydroxides from first-principles calculations. Comput Mater Sci 95:693–705. https://doi.org/10.1016/j.commatsci.2014.07.00710.1016/j.commatsci.2014.07.007 ↗
28. Wang R, Lang J, Liu Y, Lin Z, Yan X (2015) Ultra-small, size-controlled Ni(OH)2 nanoparticles: elucidating the relationship between particle size and electrochemical performance for advanced energy storage devices. NPG Asia Mater 7:e183–e183. https://doi.org/10.1038/am.2015.4210.1038/am.2015.42 ↗
29. M Singh, A Singhal (2018) Modeling of shape and size effects for the band gap of semiconductor nanoparticles. In: 2018 2nd International Conference on Micro-Electronics and Telecommunication Engineering (ICMETE), IEEE. 339–342. https://doi.org/10.1109/ICMETE.2018.0008010.1109/ICMETE.2018.00080 ↗
30. Qi WH, Wang MP, Liu QH (2005) Shape factor of nonspherical nanoparticles. J Mater Sci 40:2737–2739. https://doi.org/10.1007/s10853-005-2119-010.1007/s10853-005-2119-0 ↗
31. Samanta P (2020) Band gap engineering, quantum confinement, defect mediated broadband visible photoluminescence and associated quantum states of size tuned zinc oxide nanostructures. Optik 221:165337. https://doi.org/10.1016/j.ijleo.2020.16533710.1016/j.ijleo.2020.165337 ↗
32. Paul TC, Babu MH, Podder J, Dev BC, Sen SK, Islam S (2021) Influence of Fe3+ ions doping on TiO2 thin films: defect generation, d-d transition and band gap tuning for optoelectronic device applications. Phys B Condens Matter 604:412618. https://doi.org/10.1016/j.physb.2020.41261810.1016/j.physb.2020.412618 ↗
33. Das A, Mandal AC, Roy S, Nambissan PMG (2018) Internal defect structure of calcium doped magnesium oxide nanoparticles studied by positron annihilation spectroscopy. AIP Adv 8:095013. https://doi.org/10.1063/1.500110510.1063/1.5001105 ↗
34. Liu X, Sui B, Camargo PHC, Wang J, Sun J (2021) Tuning band gap of MnO2 nanoflowers by Alkali metal doping for enhanced Ferroptosis/phototherapy synergism in Cancer. Appl Mater Today 23:101027. https://doi.org/10.1016/j.apmt.2021.10102710.1016/j.apmt.2021.101027 ↗
35. Krishnamoorthy K, Moon JY, Hyun HB, Cho SK, Kim S-J (2012) Mechanistic investigation on the toxicity of MgO nanoparticles toward cancer cells. J Mater Chem 22:24610–24617. https://doi.org/10.1039/C2JM35087D10.1039/C2JM35087D ↗
36. Vatsha B, Tetyana P, Shumbula PM, Ngila JC, Sikhwivhilu LM, Moutloali RM (2013) Effects of precipitation temperature on nanoparticle surface area and antibacterial behaviour of Mg(OH)2 and MgO nanoparticles. J Biomater Nanobiotechnol 4:365–373. https://doi.org/10.4236/jbnb.2013.4404610.4236/jbnb.2013.44046 ↗
37. Pan X, Wang Y, Chen Z, Pan D, Cheng Y, Liu Z, Lin Z, Guan X (2013) Investigation of antibacterial activity and related mechanism of a series of nano-Mg(OH)2. ACS Appl Mater Interfaces 5:1137–1142. https://doi.org/10.1021/am302910q10.1021/am302910q ↗
38. Siqueira JF, Lopes HP (1999) Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J 32:361–369. https://doi.org/10.1046/j.1365-2591.1999.00275.x10.1046/j.1365-2591.1999.00275.x ↗
39. da Silva BL, Abuçafy MP, Manaia EB, Oshiro Junior JA, Chiari-Andréo BG, Pietro RCR, Chiavacci LA (2019) Relationship between structure and antimicrobial activity of zinc oxide nanoparticles: an overview. Int J Nanomed 14:9395–9410. https://doi.org/10.2147/IJN.S21620410.2147/IJN.S216204 ↗
40. Ponnuvelu DV, Selvaraj A, Suriyaraj SP, Selvakumar R, Pulithadathail B (2016) Ultrathin hexagonal MgO nanoflakes coated medical textiles and their enhanced antibacterial activity. Mater Res Express 3:105005. https://doi.org/10.1088/2053-1591/3/10/10500510.1088/2053-1591/3/10/105005 ↗
41. Zhang L, Yin L, Wang C, Lun N, Qi Y (2010) Sol−gel growth of hexagonal faceted ZnO prism quantum dots with polar surfaces for enhanced photocatalytic activity. ACS Appl Mater Interfaces 2:1769–1773. https://doi.org/10.1021/am100274d10.1021/am100274d ↗
42. Delgado RJR, Gasparoto TH, Sipert CR, Pinheiro CR, de Moraes IG, Garcia RB, Duarte MAH, Bramante CM, Torres SA, Garlet GP, Campanelli AP, Bernardineli N (2013) Antimicrobial activity of calcium hydroxide and chlorhexidine on intratubular Candida albicans. Int J Oral Sci 5:32–36. https://doi.org/10.1038/ijos.2013.1210.1038/ijos.2013.12 ↗
43. Ballal V, Kundabala M, Acharya S, Ballal M (2007) Antimicrobial action of calcium hydroxide, chlorhexidine and their combination on endodontic pathogens. Aust Dent J 52:118–121. https://doi.org/10.1111/j.1834-7819.2007.tb00475.x10.1111/j.1834-7819.2007.tb00475.x ↗
44. Mohammadi Z, Shalavi S, Yazdizadeh M (2012) Antimicrobial activity of calcium hydroxide in endodontics: a review. Chonnam Med J 48:133–140. https://doi.org/10.4068/cmj.2012.48.3.13310.4068/cmj.2012.48.3.133 ↗
45. Ba-Hattab R, Al-Jamie M, Aldreib H, Alessa L, Alonazi M (2016) Calcium hydroxide in endodontics: an overview. Open J Stomatol 6:274–289. https://doi.org/10.4236/ojst.2016.61203310.4236/ojst.2016.612033 ↗
46. Athanassiadis B, Walsh LJ (2017) Aspects of solvent chemistry for calcium hydroxide medicaments. Materials 10:1219. https://doi.org/10.3390/ma1010121910.3390/ma10101219 ↗
47. Kim M-A, Rosa V, Neelakantan P, Hwang Y-C, Min K-S (2021) Characterization, antimicrobial effects, and cytocompatibility of a root canal sealer produced by pozzolan reaction between calcium hydroxide and silica. Materials 14:2863. https://doi.org/10.3390/ma1411286310.3390/ma14112863 ↗
48. Nishanthi VRR (2021) Role of calcium hydroxide in dentistry: a review. Int J Pharm Res 12:2822–2827. https://doi.org/10.31838/ijpr/2020.12.02.37710.31838/ijpr/2020.12.02.377 ↗
49. Halbus AF, Horozov TS, Paunov VN (2019) Controlling the antimicrobial action of surface modified magnesium hydroxide nanoparticles. Biomimetics 4:41. https://doi.org/10.3390/biomimetics402004110.3390/biomimetics4020041 ↗
50. (2020) Relationship between pH values and molarity of acids and alkalis. https://www.aplustopper.com/relationship-ph-values-molarity-acids-alkalis/#:~:text=Conclusion%3A,will%20increase%20its%20pH%20value
51. Saliani M, Jalal R, Goharshadi EK (2015) Effects of pH and temperature on antibacterial activity of zinc oxide nanofluid against Escherichia coli O157: H7 and Staphylococcus aureus. Jundishapur J Microbiol 8:e17115. https://doi.org/10.5812/jjm.1711510.5812/jjm.17115 ↗
52. ElReash AA, Hamama H, Eldars W, Lingwei G, Zaen El-Din AM, Xiaoli X (2019) Antimicrobial activity and pH measurement of calcium silicate cements versus new bioactive resin composite restorative material. BMC Oral Health 19:235. https://doi.org/10.1186/s12903-019-0933-z10.1186/s12903-019-0933-z ↗
53. Fernández RO, Antón DN (1987) Bacteriostatic action of streptomycin on ribosomally resistant mutants (rpsL) of Salmonella typhimurium. Antimicrob Agents Chemother 31:1627–163110.1128/AAC.31.10.1627 ↗
54. Sánchez-Clemente R, Igeño MI, Población AG, Guijo MI, Merchán F, Blasco R (2018) Study of pH changes in media during bacterial growth of several environmental strains. Proceedings 2:1297. https://doi.org/10.3390/proceedings220129710.3390/proceedings2201297 ↗
55. Boudreau MA, Fisher JF, Mobashery S (2012) Messenger functions of the bacterial cell wall-derived muropeptides. Biochemistry 51:2974–2990. https://doi.org/10.1021/bi300174x10.1021/bi300174x ↗
56. Li Y, Zhang W, Niu J, Chen Y (2012) Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. ACS Nano 6:5164–5173. https://doi.org/10.1021/nn300934k10.1021/nn300934k ↗
57. Prasanna VL, Vijayaraghavan R (2015) Insight into the mechanism of antibacterial activity of ZnO: surface defects mediated reactive oxygen species even in the dark. Langmuir 31:9155–9162. https://doi.org/10.1021/acs.langmuir.5b0226610.1021/acs.langmuir.5b02266 ↗
58. Vatansever F, de Melo WCMA, Avci P, Vecchio D, Sadasivam M, Gupta A, Chandran R, Karimi M, Parizotto NA, Yin R, Tegos GP, Hamblin MR (2013) Antimicrobial strategies centered around reactive oxygen species–bactericidal antibiotics, photodynamic therapy, and beyond. FEMS Microbiol Rev 37:955–989. https://doi.org/10.1111/1574-6976.1202610.1111/1574-6976.12026 ↗
59. Halliwell B, Gutteridge JMC (2015) Free radicals in biology and medicine, 5th edn. Oxford University Press, Oxford10.1093/acprof:oso/9780198717478.001.0001 ↗
60. Godoy-Gallardo M, Eckhard U, Delgado LM, de Roo Puente YJD, Hoyos-Nogués M, Gil FJ, Perez RA (2021) Antibacterial approaches in tissue engineering using metal ions and nanoparticles: from mechanisms to applications. Bioact Mater 6:4470–4490. https://doi.org/10.1016/j.bioactmat.2021.04.03310.1016/j.bioactmat.2021.04.033 ↗
61. Wang E, Huang Y, Du Q, Sun Y (2017) Silver nanoparticle induced toxicity to human sperm by increasing ROS(reactive oxygen species) production and DNA damage. Environ Toxicol Pharmacol 52:193–199. https://doi.org/10.1016/j.etap.2017.04.01010.1016/j.etap.2017.04.010 ↗
62. de Lucca Camargo L, Touyz RM (2019) Reactive oxygen species. In: Touyz RM, Delles C (eds) Textbook of vascular medicine. Springer International Publishing, Cham, pp 127–13610.1007/978-3-030-16481-2_12 ↗
63. Kwak MS, Rhee WJ, Lee YJ, Kim HS, Kim YH, Kwon MK, Shin J-S (2021) Reactive oxygen species induce Cys106-mediated anti-parallel HMGB1 dimerization that protects against DNA damage. Redox Biol 40:101858. https://doi.org/10.1016/j.redox.2021.10185810.1016/j.redox.2021.101858 ↗