Bright red-emitting polymer dots for specific cellular imaging
Source abstract
Abstract
A new conjugated polymer, poly[{2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylphenylene)}-alt-co-{2,5-bis(N,N′-diphenylamino)-1,4-phenylene}] (DB-CN-PPV), with strong UV absorption and high fluorescence in red wavelength region, is self-assembled into nanoparticles (polymer dots, Pdots) in aqueous solution upon rapid mixing of the organic solution with water. Different-sized Pdots were obtained by varying the organic solvent percent in the mixture solution and further characterized by dynamic light scattering and transmission electron microscopy. Spectroscopic measurements indicate that the DB-CN-PPV Pdots exhibit a bright red-emitting and a quantum yield of ~27 %. Single-particle imaging and analyses indicate the brightness of DB-CN-PPV Pdots under 405 nm excitation is four times brighter than that of the well-known inorganic quantum dot probes, Qdot655, under same excitation light. The successful bioconjugation and cellular imaging suggest DB-CN-PPV Pdots have potential application for biological fluorescence imaging.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Luminescence and Fluorescent Materials · Quantum Dots Synthesis And Properties · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (39)
Show source-provided references
1. Wu C, Bull B, Szymanski C et al (2008) Multicolor conjugated polymer dots for biological fluorescence imaging. ACS Nano 2:2415–242310.1021/nn800590n ↗
2. Fass L (2008) Imaging and cancer: a review. Mol Oncol 2:115–15210.1016/j.molonc.2008.04.001 ↗
3. Giepmans BN, Adams SR, Ellisman MH et al (2006) The fluorescent toolbox for assessing protein location and function. Science 312:217–22410.1126/science.1124618 ↗
4. Kobayashi H, Ogawa M, Alford R et al (2009) New strategies for fluorescent probe design in medical diagnostic imaging. Chem Rev 110:2620–264010.1021/cr900263j ↗
5. Vu TQ, Lam WY, Hatch EW et al (2015) Quantum dots for quantitative imaging: from single molecules to tissue. Cell Tissue Res 360:71–86. doi: 10.1007/s00441-014-2087-210.1007/s00441-014-2087-2 ↗
6. Thambidurai M, Muthukumarasamy N, Agilan S et al (2010) Strong quantum confinement effect in nanocrystalline CdS. J Mater Sci 45:3254–3258. doi: 10.1007/s10853-010-4333-710.1007/s10853-010-4333-7 ↗
7. Wang Q, Seo D-K (2008) Preparation of photostable quantum dot-polystyrene microbeads through covalent organosilane coupling of CdSe@Zns quantum dots. J Mater Sci 44:816–820. doi: 10.1007/s10853-008-3138-410.1007/s10853-008-3138-4 ↗
8. Prasad KP, Chen Y, Sk MA et al (2014) Fluorescent quantum dots derived from PEDOT and their applications in optical imaging and sensing. Mater Horiz 1:529–53410.1039/C4MH00066H ↗
9. Derfus AM, Chan WC, Bhatia SN (2004) Probing the cytotoxicity of semiconductor quantum dots. Nano Lett 4:11–1810.1021/nl0347334 ↗
10. Xu H, Chen C, He Y et al (2015) Analysis of cancer marker in tissues with Hadamard transform fluorescence spectral microscopic imaging. J Fluoresc 25:397–402. doi: 10.1007/s10895-015-1525-110.1007/s10895-015-1525-1 ↗
11. Geng J, Li K, Qin W et al (2014) Red-emissive chemiluminescent nanoparticles with aggregation-induced emission characteristics for in vivo hydrogen peroxide imaging. Part Part Syst Charact 31:1238–124310.1002/ppsc.201400156 ↗
12. Sun H, Zhang F, Wei H et al (2013) The effects of composition and surface chemistry on the toxicity of quantum dots. J Mater Chem B 1:648510.1039/c3tb21151g ↗
13. Ye F, Wu C, Jin Y et al (2012) A compact and highly fluorescent orange-emitting polymer dot for specific subcellular imaging. Chem Commun 48:1778–178010.1039/c2cc16486h ↗
14. Li Q, Zhang J, Sun W et al (2014) Europium-complex-grafted polymer dots for amplified quenching and cellular imaging applications. Langmuir 30:8607–861410.1021/la501876m ↗
15. Feng G, Li K, Liu J et al (2014) Bright single-chain conjugated polymer dots embedded nanoparticles for long-term cell tracing and imaging. Small 10:1212–121910.1002/smll.201302161 ↗
16. Wu C, Szymanski C, McNeill J (2006) Preparation and encapsulation of highly fluorescent conjugated polymer nanoparticles. Langmuir 22:2956–296010.1021/la060188l ↗
17. Joshi PB, Zhang P (2015) Facile capture of conjugated polymer nanodots in silica nanoparticles to facilitate surface modification. J Mater Sci 50:3597–3603. doi: 10.1007/s10853-015-8920-510.1007/s10853-015-8920-5 ↗
18. Huang Y-C, Chen C-P, Wu P-J et al (2014) Coumarin dye-embedded semiconducting polymer dots for ratiometric sensing of fluoride ions in aqueous solution and bio-imaging in cells. J Mater Chem B 2:618810.1039/C4TB01026D ↗
19. Wu C, Schneider T, Zeigler M et al (2010) Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting. J Am Chem Soc 132:15410–1541710.1021/ja107196s ↗
20. Yu J, Wu C, Tian Z et al (2012) Tracking of single charge carriers in a conjugated polymer nanoparticle. Nano Lett 12:1300–130610.1021/nl203784m ↗
21. Wu I, Yu J, Ye F et al (2014) Squaraine-based polymer dots with narrow, bright near-infrared fluorescence for biological applications. J Am Chem Soc 137:173–17810.1021/ja5123045 ↗
22. Junkers T, Vandenbergh J, Adriaensens P et al (2012) Synthesis of poly(p-phenylene vinylene) materials via the precursor routes. Polym Chem 3:275–28510.1039/C1PY00345C ↗
23. Wu C, Szymanski C, Cain Z et al (2007) Conjugated polymer dots for multiphoton fluorescence imaging. J Am Chem Soc 129:12904–1290510.1021/ja074590d ↗
24. Adebanjo O, Maharjan PP, Adhikary P et al (2013) Triple junction polymer solar cells. Energy Environ Sci 6:3150–317010.1039/c3ee42257g ↗
25. Wu C, Hansen SJ, Hou Q et al (2011) Design of highly emissive polymer dot bioconjugates for in vivo tumor targeting. Angew Chem Int Ed 50:3430–343410.1002/anie.201007461 ↗
26. Wu C, Chiu DT (2013) Highly fluorescent semiconducting polymer dots for biology and medicine. Angew Chem Int Ed 52:3086–310910.1002/anie.201205133 ↗
27. Chang K, Liu Z, Chen H et al (2014) Conjugated polymer dots for ultra-stable full-color fluorescence patterning. Small 10:4270–427510.1002/smll.201401436 ↗
28. Wu C, Jin Y, Schneider T et al (2010) Ultrabright and bioorthogonal labeling of cellular targets using semiconducting polymer dots and click chemistry. Angew Chem Int Ed 49:9436–944010.1002/anie.201004260 ↗
29. Sun K, Chen H, Wang L et al (2014) Size-dependent property and cell labeling of semiconducting polymer dots. ACS Appl Mater Interfaces 6:10802–1081210.1021/am502733n ↗
30. Wu Y, Shi M, Zhao L et al (2014) Visible-light-excited and europium-emissive nanoparticles for highly-luminescent bioimaging in vivo. Biomaterials 35:5830–583910.1016/j.biomaterials.2014.03.080 ↗
31. Zhang X, Yu J, Rong Y et al (2013) High-intensity near-ir fluorescence in semiconducting polymer dots achieved by cascade fret strategy. Chem Sci 4:2143–215110.1039/c3sc50222h ↗
32. Pei Q, Yang Y (1996) Efficient photoluminescence and electroluminescence from a soluble polyfluorene. J Am Chem Soc 118:7416–741710.1021/ja9615233 ↗
33. Liu Z, Sun Z, Di W et al (2015) Brightness calibrates particle size in single particle fluorescence imaging. Opt Lett 40:1242–124510.1364/OL.40.001242 ↗
34. He C, Hu Y, Yin L et al (2010) Effects of particle size and surface charge on cellular uptake and biodistribution of polymeric nanoparticles. Biomaterials 31:3657–366610.1016/j.biomaterials.2010.01.065 ↗
35. Li Q, Sun K, Chang K et al (2013) Ratiometric luminescent detection of bacterial spores with terbium chelated semiconducting polymer dots. Anal Chem 85:9087–909110.1021/ac4016616 ↗
36. Beta M, Khetan V, Chatterjee N et al (2014) EpCAM knockdown alters microRNA expression in retinoblastoma: functional implication of EpCAM regulated miRNA in tumor progression. PLoS One 9:e11480010.1371/journal.pone.0114800 ↗
37. Ligtenberg MJ, Kuiper RP, Geurts van Kessel A et al (2013) EPCAM deletion carriers constitute a unique subgroup of Lynch syndrome patients. Fam Cancer 12:169–17410.1007/s10689-012-9591-x ↗
38. Feng G, Liu J, Zhang R et al (2014) Cell imaging using red fluorescent light-up probes based on an environment-sensitive fluorogen with intramolecular charge transfer characteristics. Chem Commun 50:9497–950010.1039/C4CC04654D ↗
39. Fernando LP, Kandel PK, Yu J et al (2010) Mechanism of cellular uptake of highly fluorescent conjugated polymer nanoparticles. Biomacromolecules 11:2675–268210.1021/bm1007103 ↗