A comparison study on bioactivity and antibacterial properties of Ag-, Cu- and Zn- deposited oxide coatings produced on titanium
Source abstract
Abstract
The aim of this study was to create antimicrobial and bioactive surfaces on the commercial pure titanium (cp-Ti) surface for dental and orthopedic applications. Antibacterial and bioactive silver (Ag)-, copper (Cu)- and zinc (Zn)-based TiO2 with thicknesses of 1, 3 and 5 nm coatings were produced on cp-Ti surfaces via micro arc oxidation (MAO) and thermal evaporation (PVD-TE) techniques. The TiO2, brookite-TiO2 and rutile-TiO2 phases were detected on MAO treated cp-Ti plates and PVD-TE coated MAO surfaces. The surfaces were porous and rough owing to the presence of micro sparks that occurred through the MAO process. However, the surface morphology was not altered at post-coating PVD-TE process although the surface chemistry changed. The Ti, O, Si, Ag, Cu and Zn were homogeneously distributed through the surface and were observed on thin film-coated surfaces. The surfaces of MAO treated cp-Ti plates showed hydrophilic properties, while the thin film-coated MAO surfaces indicated hydrophobic properties. To investigate the bioactivities of Ag-, Cu- and Zn- deposited oxide coatings produced on titanium; all samples were soaked into 1.0X simulated body fluid (SBF) for 14 days at 36.5 °C. Whlie Cu surfaces showed better bioactivity properties compared to Ag, Zn surfaces revealed the best bioactivity. The antibacterial activities of plain MAO and Ag-, Cu- and Zn-coated MAO surfaces were evaluated against Escherichia coli and Staphylococcus aureus the colony counting method. Antibacterial studies concluded that Ag, Cu and Zn deposition effectively improved the antibacterial properties of MAO surface. When the coated thin film were compared, it was observed that Ag possessed the most effective antibacterial property against E. coli and S. aureus. Additionally, Zn-based coating revealed better antibacterial efficiency compared to Cu-based coating.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Dental materials and restorations · Dental Implant Techniques and Outcomes
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (60)
Show source-provided references
1. Liu ZQ, Liu XL, Ramakrishna S (2021) Surface engineering of biomaterials in orthopedic and dental implants: strategies to improve osteointegration, bacteriostatic and bactericidal activities. Biotechnol J 16:200011610.1002/biot.202000116 ↗
2. Chouirfa H, Bouloussa H, Migonney V, Falentin-Daudre C (2019) Review of titanium surface modification techniques and coatings for antibacterial applications. Acta Biomater 83:37–5410.1016/j.actbio.2018.10.036 ↗
3. Ju K, Chen X, Zhao Z (2022) Fabrication of Ti/TiO2(Ca)/hydroxyapatite bioceramic material by micro-arc oxidation and electrochemical deposition. Ceram Int 48:19937–1994310.1016/j.ceramint.2022.03.268 ↗
4. Yang TS, Huang MS, Wang MS, Lin MH, Tsai MY, Wang PYW (2013) Effect of electrical discharging on formation of nanoporous biocompatible layer on Ti–6Al–4V alloys. Implant Dent 22:374–37910.1097/ID.0b013e31829a170a ↗
5. Asserghine A, Filotás D, Németh B, Nagy L, Nagy G (2018) Potentiometric scanning electrochemical microscopy for monitoring the pH distribution during the self-healing of passive titanium dioxide layer on titanium dental root implant exposed to physiological buffered (PBS) medium. Electrochem Commun 95:1–410.1016/j.elecom.2018.08.008 ↗
6. Drnovsek N, Rade K, Milacic R, Strancar J, Novak S (2012) The properties of bioactive TiO2 coatings on Ti-based implants. Surf Coat Technol 209:177–18310.1016/j.surfcoat.2012.08.037 ↗
7. Masmoudi M, Assoul M, Wery M, Abdelhedi R, El Halouani F, Monteil G (2006) Friction and wear behaviour of cp Ti and Ti6Al4V following nitric acid passivation. Appl Surf Sci 253:2237–224310.1016/j.apsusc.2006.04.047 ↗
8. Amin Yavari S, van der Stok J, Chai YC, Wauthle R, TahmasebiBirgani Z, Habibovic P, Mulier M, Schrooten J, Weinans H, Zadpoor AA (2014) Bone regeneration performance of surface-treated porous titanium. Biomaterials 35:6172–618110.1016/j.biomaterials.2014.04.054 ↗
9. Nogueira RP, Uchoa JD, Hilario F, Santana-Melo GD, de Vasconcellos LMR, Marciano FR, Roche V, Jorge AM, Lobo AO (2021) Characterization of optimized TiO2 nanotubes morphology for medical implants: biological activity and corrosion resistance. Int J Nanomed 16:667–68210.2147/IJN.S285805 ↗
10. Acciari HA, Palma DPS, Codaro EN, Zhou Q, Wang J, Ling Y, Zhang J, Zhang Z (2019) Surface modifications by both anodic oxidation and ion beam implantation on electropolished titanium substrates. Appl Surf Sci 487:1111–112010.1016/j.apsusc.2019.05.216 ↗
11. Sasikumar Y, Indira K, Rajendran N (2019) Surface modification methods for titanium and its alloys and their corrosion behavior in biological environment: a review. J Bio- Tribo-Corros 5:3610.1007/s40735-019-0229-5 ↗
12. Zhou R, Wei DQ, Cao JY, Feng W, Cheng S, Du Q, Li BQ, Wang YM, Jia DC, Zhou Y (2015) Synergistic effects of surface chemistry and topologic structure from modified microarc oxidation coatings on Ti implants for improving osseointegration. ACS Appl Mater Interfaces 7:8932–894110.1021/acsami.5b02226 ↗
13. Jiang BL, Wang YM (2010) Plasma electrolytic oxidation treatment of aluminium and titanium alloys. In: Dong H (ed) Surface engineering of light alloys. Woodhead Publishing, Sawston
14. Nikoomanzari E, Karbasi M, Melo WCMA, Moris H, Babaei K, Giannakis S, Fattah-alhosseini A (2022) Impressive strides in antibacterial performance amelioration of Ti-based implants via plasma electrolytic oxidation (PEO): a review of the recent advancements. Chem Eng J 441:13600310.1016/j.cej.2022.136003 ↗
15. Darouiche RO (2004) Current concepts—treatment of infections associated with surgical implants. N Engl J Med 350:1422–142910.1056/NEJMra035415 ↗
16. Mouhyi J, Dohan Ehrenfest DM, Albrektsson T (2012) The peri-implantitis: implant surfaces, microstructure, and physicochemical aspects. Clin Implant Dentistry Related Res 14:170–18310.1111/j.1708-8208.2009.00244.x ↗
17. Aktug SL, Durdu S, Aktas S, Yalcin E, Usta M (2019) Characterization and investigation of in vitro properties of antibacterial copper deposited on bioactive ZrO2 coatings on zirconium. Thin Solid Films 681:69–7710.1016/j.tsf.2019.04.042 ↗
18. Durdu S, Aktug SL, Aktas S, Yalcin E, Usta M (2018) Fabrication and in vitro properties of zinc-based superhydrophilic bioceramic coatings on zirconium. Surf Coat Technol 344:467–47810.1016/j.surfcoat.2018.03.062 ↗
19. Durdu S, Aktug SL, Aktas S, Yalcin E, Cavusoglu K, Altinkok A, Usta M (2017) Characterization and in vitro properties of anti-bacterial Ag-based bioceramic coatings formed on zirconium by micro arc oxidation and thermal evaporation. Surf Coat Technol 331:107–11510.1016/j.surfcoat.2017.10.023 ↗
20. Yu WZ, Zhang YZ, Liu XM, Xiang YM, Li ZY, Wu SL (2018) Synergistic antibacterial activity of multi components in lysozyme/chitosan/silver/hydroxyapatite hybrid coating. Mater Des 139:351–36210.1016/j.matdes.2017.11.018 ↗
21. Taylor AA, Tsuji JS, Garry MR, McArdle ME, Goodfellow WL, Adams WJ, Menzie CA (2020) Critical review of exposure and effects: implications for setting regulatory health criteria for ingested copper. Environ Manage 65:131–15910.1007/s00267-019-01234-y ↗
22. Duffy LL, Osmond-McLeod MJ, Judy J, King T (2018) Investigation into the antibacterial activity of silver, zinc oxide and copper oxide nanoparticles against poultry-relevant isolates of Salmonella and Campylobacter. Food Control 92:293–30010.1016/j.foodcont.2018.05.008 ↗
23. Zhang EL, Zhao XT, Hu JL, Wang RX, Fu S, Qin GW (2021) Antibacterial metals and alloys for potential biomedical implants. Bioactive Materials 6:2569–261210.1016/j.bioactmat.2021.01.030 ↗
24. Page K, Wilson M, Parkin IP (2009) Antimicrobial surfaces and their potential in reducing the role of the inanimate environment in the incidence of hospital-acquired infections. J Mater Chem 19:3819–383110.1039/b818698g ↗
25. Chaloupka K, Malam Y, Seifalian AM (2010) Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol 28:580–58810.1016/j.tibtech.2010.07.006 ↗
26. Eckhardt S, Brunetto PS, Gagnon J, Priebe M, Giese B, Fromm KM (2013) Nanobio silver: its interactions with peptides and bacteria, and its uses in medicine. Chem Rev 113:4708–475410.1021/cr300288v ↗
27. Lemire JA, Harrison JJ, Turner RJ (2013) Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat Rev Microbiol 11:371–38410.1038/nrmicro3028 ↗
28. Zhang JC, Li YP, Yang KN, Hao XH (2010) Effects of Cu2+ and Cu+ on the proliferation, differentiation and calcification of primary mouse osteoblasts in vitro. Chin J Inorg Chem 26:2251–2258
29. Shi F, Liu Y, Zhi W, Xiao D, Li H, Duan K, Qu S, Weng J (2017) The synergistic effect of micro/nano-structured and Cu2+-doped hydroxyapatite particles to promote osteoblast viability and antibacterial activity. Biomed Mater (Bristion) 12:03500610.1088/1748-605X/aa6c8d ↗
30. Almoudi MM, Hussein AS, Abu Hassan MI, Mohamad Zain N (2018) A systematic review on antibacterial activity of zinc against Streptococcus mutans. Saudi Dental J 30:283–29110.1016/j.sdentj.2018.06.003 ↗
31. Lynch RJM (2011) Zinc in the mouth, its interactions with dental enamel and possible effects on caries; a review of the literature. Int Dent J 61:46–5410.1111/j.1875-595X.2011.00049.x ↗
32. Petrini P, Arciola CR, Pezzali I, Bozzini S, Montanaro L, Tanzi MC, Speziale P, Visai L (2006) Antibacterial activity of zinc modified titanium oxide surface. Int J Artif Organs 29:434–44210.1177/039139880602900414 ↗
33. Zhang X, Wang H, Li J, He X, Hang R, Huang X, Tian L, Tang B (2016) Corrosion behavior of Zn-incorporated antibacterial TiO2 porous coating on titanium. Ceram Int 42:17095–1710010.1016/j.ceramint.2016.07.220 ↗
34. Wang R, He X, Gao Y, Zhang X, Yao X, Tang B (2017) Antimicrobial property, cytocompatibility and corrosion resistance of Zn-doped ZrO2/TiO2 coatings on Ti6Al4V implants. Mater Sci Eng C 75:7–1510.1016/j.msec.2017.02.036 ↗
35. Yoo MH, Lee JY, Lee SE, Koh JY, Yoon YH (2004) Protection by pyruvate of rat retinal cells against zinc toxicity in vitro, and pressure-induced ischemia in vivo. Invest Ophthalmol Vis Sci 45:1523–153010.1167/iovs.03-1315 ↗
36. Jin G, Qin H, Cao H, Qian S, Zhao Y, Peng X, Zhang X, Liu X, Chu PK (2014) Synergistic effects of dual Zn/Ag ion implantation in osteogenic activity and antibacterial ability of titanium. Biomaterials 35:7699–771310.1016/j.biomaterials.2014.05.074 ↗
37. Shimabukuro M, Tsutsumi H, Tsutsumi Y, Manaka T, Chen P, Ashida M, Ishikawa K, Katayama H, Hanawa T (2021) Enhancement of antibacterial property of titanium by two-step micro arc oxidation treatment. Dent Mater J 40:592–59810.4012/dmj.2020-188 ↗
38. Zhang YY, Zhu Y, Lu DZ, Dong W, Bi WJ, Feng XJ, Wen LM, Sun H, Qi MC (2021) Evaluation of osteogenic and antibacterial properties of strontium/silver-containing porousTiO(2)coatings prepared by micro-arc oxidation. J Biomed Mater Res Part B Appl Biomaterials 109:505–51610.1002/jbm.b.34719 ↗
39. Lv Y, Zhang T, Zhang XX, Fu S, Yang L, Dong ZH, Ma YL, Zhang EL (2021) The synergistic effect of Ag and ZnO on the microstructure, corrosion resistance and in vitro biological performance of titania coating. Surf Coat Technol 426:12779810.1016/j.surfcoat.2021.127798 ↗
40. Sedelnikova MB, Komarova EG, Sharkeev YP, Ugodchikova AV, Mushtovatova LS, Karpova MR, Sheikin VV, Litvinova LS, Khlusov IA (2019) Zn-, Cu- or Ag-incorporated micro-arc coatings on titanium alloys: Properties and behavior in synthetic biological media. Surf Coat Technol 369:52–6810.1016/j.surfcoat.2019.04.021 ↗
41. Zhang XX, Zhang T, Lv Y, Zhang YP, Lu XQ, Xiao JY, Ma C, Li Z, Dong ZH (2021) Enhanced uniformity, corrosion resistance and biological performance of Cu-incorporated TiO2 coating produced by ultrasound-auxiliary micro-arc oxidation. Appl Surf Sci 569:15093210.1016/j.apsusc.2021.150932 ↗
42. Huang QL, Liu XJ, Zhang RR, Yang X, Lan CW, Feng QL, Liu Y (2019) The development of Cu-incorporated micro/nano-topographical bio-ceramic coatings for enhanced osteoblast response. Appl Surf Sci 465:575–58310.1016/j.apsusc.2018.09.208 ↗
43. Sun H, Yang Y, Yu L, Liu K, Fei Y, Guo C, Zhou Y, Hu J, Shi L, Ji H (2022) Inhibition of inflammatory response and promotion of osteogenic Activity of zinc-doped micro-arc titanium oxide coatings. ACS Omega 7:14920–1493210.1021/acsomega.2c00579 ↗
44. Durdu S, Aktug SL, Korkmaz K, Yalcin E, Aktas S (2018) Fabrication, characterization and in vitro properties of silver-incorporated TiO2 coatings on titanium by thermal evaporation and micro-arc oxidation. Surf Coat Technol 352:600–60810.1016/j.surfcoat.2018.08.050 ↗
45. Durdu S (2019) Characterization, bioactivity and antibacterial properties of copper-based TiO2 bioceramic coatings fabricated on titanium. Coatings 9:110.3390/coatings9010001 ↗
46. Li Q, Yang W, Liu C, Wang D, Liang J (2017) Correlations between the growth mechanism and properties of micro-arc oxidation coatings on titanium alloy: effects of electrolytes. Surf Coat Technol 316:162–17010.1016/j.surfcoat.2017.03.021 ↗
47. Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27:2907–291510.1016/j.biomaterials.2006.01.017 ↗
48. Xu L, Wu C, Lei X, Zhang K, Liu C, Ding J, Shi X (2018) Effect of oxidation time on cytocompatibility of ultrafine-grained pure Ti in micro-arc oxidation treatment. Surf Coat Technol 342:12–2210.1016/j.surfcoat.2018.02.044 ↗
49. Demirbaş Ç, Ayday A (2021) Effect of Ag concentration on structure and wear behaviour of coatings formed by micro-arc oxidation on Ti6Al4 V Alloy. Surf Eng 37:24–3110.1080/02670844.2020.1741211 ↗
50. Zhang X, Cai G, Lv Y, Wu Y, Dong Z (2020) Growth mechanism of titania on titanium substrate during the early stage of plasma electrolytic oxidation. Surf Coat Technol 400:12620210.1016/j.surfcoat.2020.126202 ↗
51. Liu SM, Li BE, Liang CY, Wang HS, Qiao ZX (2016) Formation mechanism and adhesive strength of a hydroxyapatite/TiO2 composite coating on a titanium surface prepared by micro-arc oxidation. Appl Surf Sci 362:109–11410.1016/j.apsusc.2015.11.086 ↗
52. Bico J, Thiele U, Quere D (2002) Wetting of textured surfaces. Coll Surf A Physicochem Eng Asp 206:41–4610.1016/S0927-7757(02)00061-4 ↗
53. Gordon O, Slenters TV, Brunetto PS, Villaruz AE, Sturdevant DE, Otto M, Landmann R, Fromm KM (2010) Silver coordination polymers for prevention of implant infection: thiol interaction, impact on respiratory chain enzymes, and hydroxyl radical induction. Antimicrob Agents Chemother 54:4208–421810.1128/AAC.01830-09 ↗
54. Bai X, Sandukas S, Appleford M, Ong JL, Rabiei A (2012) Antibacterial effect and cytotoxicity of Ag-doped functionally graded hydroxyapatite coatings. J Biomed Mater Res Part B-Appl Biomater 100B:553–56110.1002/jbm.b.31985 ↗
55. Pasquet J, Chevalier Y, Couval E, Bouvier D, Bolzinger MA (2015) Zinc oxide as a new antimicrobial preservative of topical products: Interactions with common formulation ingredients. Int J Pharm 479:88–9510.1016/j.ijpharm.2014.12.031 ↗
56. Wang C, Liu LL, Zhang AT, Xie P, Lu JJ, Zou XT (2012) Antibacterial effects of zinc oxide nanoparticles on Escherichia coli. Afric J Biotechnol 11:10248–10254
57. Pietrzyk B, Porebska K, Jakubowski W, Miszczak S (2019) Antibacterial properties of Zn doped hydrophobic SiO2 coatings produced by sol–gel method. Coatings 9:36210.3390/coatings9060362 ↗
58. Grass G, Rensing C, Solioz M (2011) Metallic copper as an antimicrobial surface. Appl Environ Microbiol 77:1541–154710.1128/AEM.02766-10 ↗
59. Cobine PA, Pierrel F, Winge DR (2006) Copper trafficking to the mitochondrion and assembly of copper metalloenzymes. Biochim Biophys Acta Mol Cell Res 1763:759–77210.1016/j.bbamcr.2006.03.002 ↗
60. Bondarczuk K, Piotrowska-Seget Z (2013) Molecular basis of active copper resistance mechanisms in Gram-negative bacteria. Cell Biol Toxicol 29:397–40510.1007/s10565-013-9262-1 ↗