Effect of amino-functionalization on insulin delivery and cell viability for two types of silica mesoporous structures
Source abstract
Abstract
Inorganic mesoporous structures are a class of novel biomaterials that have shown practical applications in delivery of a variety of therapeutic agents. In the present study, two mesoporous structures were prepared, and the effect of surface modification on their insulin delivery and in vitro cytotoxicity was evaluated. Morphological and structural characterizations of silica particles were accomplished by different analytical techniques, including scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), and Brunauer–Emmett–Teller (BET) surface area analyses. The drug loading capacity and in vitro drug release behavior of silica structures were investigated under simulated gastrointestinal conditions and phosphate-buffered saline solution using FTIR and UV–Vis spectroscopy. In vitro cytotoxicity evaluation was carried out via MTT assay. Results showed that the morphology of MCM-41 was round, while SBA-15 was wheat like, both possessed almost homogeneous size distribution. Also, modification with amine did not influence the morphology and structure of the particles. Both MCM-41 and SBA-15 particles were found to have narrow pore-size distributions of 2.8 and 6.8 nm, respectively. SBA-15 particles demonstrated a high insulin loading capacity of about 15.1 %, while MCM-41 and modified MCM-41 (mMCM-41) were observed to load virtually no insulin at all. The surface modification by amino groups resulted in higher insulin loading and the slower rate of release for modified SBA-15 (mSBA-15) compared to the non-modified SBA-15 (SBA-15). According to the cytotoxicity evaluation results, all of the samples showed cytotoxicity Grade 0–1, in a concentration-dependent manner. Moreover, insulin-loaded mSBA-15 particles exhibited higher cell viability compared to the others. It was concluded that amine modification of SBA-15 could result in higher loading and extended release of insulin and more cell viability.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Mesoporous Materials and Catalysis · Bone Tissue Engineering Materials · Layered Double Hydroxides Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (59)
Show source-provided references
1. Webster JG (2006) Encyclopedia of medical devices and instrumentation, 2nd edn. Wiley, New York10.1002/0471732877 ↗
2. Yokoi T, Kubota Y, Tatsumi T (2012) Amino-functionalized mesoporous silica as base catalyst and adsorbent. Appl Catal A 42:14–37. doi: 10.1016/j.apcata.2012.02.00410.1016/j.apcata.2012.02.004 ↗
3. Chung J, Chun J, Lee J, Lee SH, Lee YJ, Hong SW (2012) Sorption of Pb(II) and Cu(II) onto multi-amine grafted mesoporous silica embedded with nano-magnetite: effects of steric factors. J Hazard Mater 239–240:183–191. doi: 10.1016/j.jhazmat.2012.08.06310.1016/j.jhazmat.2012.08.063 ↗
4. Cabot A, Arbiol J, Cornet A, Morante JR, Chen F, Liu M (2003) Mesoporous catalytic filters for semiconductor gas sensors. Thin Solid Films 436:64–69. doi: 10.1016/S0040-6090(03)00510-810.1016/S0040-6090(03)00510-8 ↗
5. Tomer VK, Adhyapak PV, Duhan S, Mulla IS (2014) Humidity sensing properties of Ag-loaded mesoporous silica SBA-15 nanocomposites prepared via hydrothermal process. Microporous Mesoporous Mater 197:140–147. doi: 10.1016/j.micromeso.2014.06.00710.1016/j.micromeso.2014.06.007 ↗
6. Börgardts M, Verlinden K, Neidhardt M, Wöhrle T, Herbst A, Laschat S, Janiak C, MüllerSynthesis TJJ (2016) Synthesis and optical properties of covalently bound Nile Red in mesoporous silica hybrids—comparison of dye distribution of materials prepared by facile grafting and by co-condensation routes. RSC Adv 6:6209–6222. doi: 10.1039/C5RA22736D10.1039/C5RA22736D ↗
7. Kempen PJ, Greasley S, Parker KA, Campbell JL, Chang HY, Jones JR, Sinclair R, Gambhir SS, Jokerst JV (2015) Theranostic mesoporous silica nanoparticles biodegrade after pro-survival drug delivery and ultrasound/magnetic resonance imaging of stem cells. Theranostics 5:631–642. doi: 10.7150/thno.1138910.7150/thno.11389 ↗
8. Maggini L, Cabrera I, Ruiz-Carretero A, Prasetyanto E, Robinet E, De Cola L (2016) Breakable mesoporous silica nanoparticles for targeted drug delivery. Nanoscale 7:7240–7247. doi: 10.1039/C5NR09112H10.1039/C5NR09112H ↗
9. He Q, Gao Y, Zhang L, Zhang Z, GaoF Ji X, Li Y, Shi J (2011) A pH-responsive mesoporous silica nanoparticles-based multi-drug delivery system for overcoming multi-drug resistance. Biomaterials 32:7711–7720. doi: 10.1016/j.biomaterials.2011.06.06610.1016/j.biomaterials.2011.06.066 ↗
10. Wang S (2009) Ordered mesoporous materials for drug delivery. Microporous Mesoporous Mater 117:1–9. doi: 10.1016/j.micromeso.2008.07.00210.1016/j.micromeso.2008.07.002 ↗
11. Wang S, Wang X, Draenert FG, Albert O, Schroder HC, Mailander V, Mitov G, Muller WE (2014) Bioactive and biodegradable silica biomaterial for bone regeneration. Bone 67:292–304. doi: 10.1016/j.bone.2014.07.02510.1016/j.bone.2014.07.025 ↗
12. Persson C, Unosson E, Ajaxon I, Engstrand J, Engqvist H, Xia W (2012) Nano grain sized zirconia–silica glass ceramics for dental applications. J Eur Ceram Soc 32:4105–4110. doi: 10.1016/j.jeurceramsoc.2012.06.02810.1016/j.jeurceramsoc.2012.06.028 ↗
13. Li J, Qin X, Yang Z, Qi H, Xu Q, Diao G (2013) A novel mesoporous silica nanosphere matrix for the immobilization of proteins and their applications as electrochemical biosensor. Talanta 104:116–121. doi: 10.1016/j.talanta.2012.11.03810.1016/j.talanta.2012.11.038 ↗
14. Xia L, Zeng D, Sun X, Xu Y, Xu L, Ye D, Zhang X, Jiang X, Zhang Z (2013) Engineering of bone using rhBMP-2-loaded mesoporous silica bioglass and bone marrow stromal cells for oromaxillofacial bone regeneration. Microporous Mesoporous Mater 173:155–165. doi: 10.1016/j.micromeso.2013.02.02010.1016/j.micromeso.2013.02.020 ↗
15. Mamaeva V, Sahlgren C, Lindén M (2013) Mesoporous silica nanoparticles in medicine—Recent advances. Adv Drug Deliv Rev 65:689–702. doi: 10.1016/j.addr.2012.07.01810.1016/j.addr.2012.07.018 ↗
16. Gimenez C, de la Torre C, Gorbe M, Aznar E, Sancenón F, Murguía JR, Martínez-Máñez R, Marcos MD, Amorós P (2015) Gated mesoporous silica nanoparticles for the controlled delivery of drugs in cancer cells. Langmuir 31:3753–3762. doi: 10.1021/acs.langmuir.5b0013910.1021/acs.langmuir.5b00139 ↗
17. Bhattacharyya S, Wang H, Ducheyne P (2012) Polymer-coated mesoporous silica nanoparticles for the controlled release of macromolecules. Acta Biomater 8:3429–3435. doi: 10.1016/j.actbio.2012.06.00310.1016/j.actbio.2012.06.003 ↗
18. Xue J, Shi M (2004) PLGA/mesoporous silica hybrid structure for controlled drug release. J Controlled Release 98:209–217. doi: 10.1016/j.jconrel.2004.04.02310.1016/j.jconrel.2004.04.023 ↗
19. Chen X, Yao X, Wang C, Chen L, Chen X (2015) Mesoporous silica nanoparticles capped with fluorescence-conjugated cyclodextrin for pH-activated controlled drug delivery and imaging. Microporous Mesoporous Mater 217:46–53. doi: 10.1016/j.micromeso.2015.06.01210.1016/j.micromeso.2015.06.012 ↗
20. Qu Y, Feng L, Tong C, Liu B, Lu C (2013) Poly (p-phenylenevinylene) functionalized fluorescent mesoporous silica nanoparticles for drug release and cell imaging. Microporous Mesoporous Mater 182:155–164. doi: 10.1016/j.micromeso.2013.08.04510.1016/j.micromeso.2013.08.045 ↗
21. Slowing II, Vivero-Escoto JL, Wu CW, Lin VS (2008) Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers. Adv Drug Deliv Rev 60:1278–1288. doi: 10.1016/j.addr.2008.03.01210.1016/j.addr.2008.03.012 ↗
22. Xu Y, Wang C, Zhou G, Wu Y, Chen J (2012) Improving the controlled release of water-insoluble emodin from amino-functionalized mesoporous silica. Appl Surf Sci 258:6366–6372. doi: 10.1016/j.apsusc.2012.03.04110.1016/j.apsusc.2012.03.041 ↗
23. Doardio AL, Sousa SE, Doadrio JC, Pariente JP, Izquierdo-Barba I, Vallet-Regi M (2014) Mesoporous SBA-15 HPLC evaluation for controlled gentamicin drug delivery. J Controlled Release 97:125–132. doi: 10.1016/j.jconrel.2004.03.00510.1016/j.jconrel.2004.03.005 ↗
24. Ghedini E, Signoretto M, Pinna F, Guarascio D, Cerrato G (2008) Ibuprofen delivery behaviour on MCM–41: influence of organic groups amount. Stud Surf Sci Catal 174:429–432. doi: 10.1016/S0167-2991(08)80234-010.1016/S0167-2991(08)80234-0 ↗
25. Airoldi C, Oliveira VV (2014) Hydrophobic contribution to amoxicillin release associated with organofunctionalized mesoporous SBA-16 carriers. Mater Res Bull 59:214–222. doi: 10.1016/j.materresbull.2014.06.03110.1016/j.materresbull.2014.06.031 ↗
26. Huang W, Liu W, She Z, Wu H, Shi X (2011) Alendronate decorated nano hydroxyapatite in mesoporous silica: cytotoxicity and osteogenic properties. Appl Surf Sci 257:9757–9761. doi: 10.1016/j.apsusc.2011.06.00210.1016/j.apsusc.2011.06.002 ↗
27. Al-Kady AS, Gaber M, Hussein MM, Ebeid EZM (2011) Nanostructure-loaded mesoporous silica for controlled release of coumarin derivatives: a novel testing of the hyperthermia effect. Eur J Pharm Biopharm 77:66–74. doi: 10.1016/j.ejpb.2010.10.00710.1016/j.ejpb.2010.10.007 ↗
28. Wang H, Gao X, Wang Y, Wang J, Niu X, Deng X (2012) Effect of amine functionalization of SBA-15 on controlled baicalin drug release. Ceram Int 38:6931–6935. doi: 10.1016/j.ceramint.2012.05.06210.1016/j.ceramint.2012.05.062 ↗
29. Mahato RI, Narang AS, Thoma L, Miller DD (2003) Emerging trends in oral delivery of peptide and protein drugs. Crit Rev Ther Drug Carr Syst 20:164–167. doi: 10.1615/CritRevTherDrugCarrierSyst.v20.i23.3010.1615/CritRevTherDrugCarrierSyst.v20.i23.30 ↗
30. Wang H, Zhao P, Su W, Wang S, Liao Z, Niu R, Chang J (2010) PLGA/polymeric liposome for targeted drug and gene co-delivery. Biomaterials 31:8741–8748. doi: 10.1016/j.biomaterials.2010.07.08210.1016/j.biomaterials.2010.07.082 ↗
31. Forier K, Raemdonck K, De Smedt SC, Demeester J, Coenye T, Braeckmans K (2014) Lipid and polymer nanoparticles for drug delivery to bacterial biofilms. J Controlled Release 190:607–623. doi: 10.1016/j.jconrel.2014.03.05510.1016/j.jconrel.2014.03.055 ↗
32. Park K (2014) Controlled drug delivery systems: past forward and future back. J Controlled Release 190:3–8. doi: 10.1016/j.jconrel.2014.03.05410.1016/j.jconrel.2014.03.054 ↗
33. Andreani T, Miziara L, Lorenzón EN, de Souza AL, Kiill CP, Fangueiro JF, Garcia ML, Gremião PD, Silva AM, Souto EB (2015) Effect of mucoadhesive polymers on the in vitro performance of insulin-loaded silica nanoparticles: interactions with mucin and biomembrane models. Eur J Pharm Biopharm 93:118–126. doi: 10.1016/j.ejpb.2015.03.02710.1016/j.ejpb.2015.03.027 ↗
34. Andreani T, Kiill CP, de Souza AL, Fangueiro JF, Fernandes L, Doktorovová S, Santos DL, Garcia ML, Gremião MP, Souto EB, Silva AM (2014) Surface engineering of silica nanoparticles for oral insulin delivery: characterization and cell toxicity studie. Colloids Surf B 123:916–923. doi: 10.1016/j.colsurfb.2014.10.04710.1016/j.colsurfb.2014.10.047 ↗
35. Elsayed A, Al-Remawi M, Maghrabi I, Hamaidi M, Jaber N (2014) Development of insulin loaded mesoporous silica injectable particles layered by chitosan as a controlled release delivery system. Int J Pharm 461:448–458. doi: 10.1016/j.ijpharm.2013.12.01410.1016/j.ijpharm.2013.12.014 ↗
36. Lin YH, Chen YP, Liu TP, Chien FC, Chou CM, Chen CT, Mou CY (2016) Approach To Deliver Two Antioxidant Enzymes with Mesoporous Silica Nanoparticles into Cells. ACS Appl Mater Interfaces 8:17944–17954. doi: 10.1021/acsami.6b0583410.1021/acsami.6b05834 ↗
37. Tukappa A, Ultimo A, da la Torre C, Pardo T, Sancenon F, Martinez-Manez R (2016) Polyglutamic acid-gated mesoporous silica nanoparticles for enzyme-controlled drug delivery. Langmuir. doi: 10.1021/acs.langmuir.6b0171510.1021/acs.langmuir.6b01715 ↗
38. Gan Q, Zhu J, Yuan Y, Liu C (2016) pH-Responsive Fe3O4 nanopartilces-capped mesoporous silica supports for protein delivery. J Nanosci Nanotechnol 16:5470–5479. doi: 10.1166/jnn.2016.1174410.1166/jnn.2016.11744 ↗
39. Siavashani AZ, Nazarpak MH, Fayyazbakhsh F, Toliyat T, Solati-Hashjin M (2014) Preparation of mesoporous silica nanoparticles for insulin drug delivery. Adv Mater Res 829:251–257. doi: 10.4028/www.scientific.net/AMR.829.25110.4028/www.scientific.net/AMR.829.251 ↗
40. Chen F, Zhu Y (2012) Chitosan enclosed mesoporous silica nanoparticles as drug nano-carriers: sensitive response to the narrow pH range. Microporous Mesoporous Mater 150:83–89. doi: 10.1016/j.micromeso.2011.07.02310.1016/j.micromeso.2011.07.023 ↗
41. Zhang Y, Wei W, Lv P, Wang L, Ma G (2011) Preparation and evaluation of alginate–chitosan microspheres for oral delivery of insulin. Eur J Pharm Biopharm 77:9–11. doi: 10.1016/j.ejpb.2010.09.01610.1016/j.ejpb.2010.09.016 ↗
42. Vallet-Regí M, Balas F (2008) Silica materials for medical applications. Open Biomed Eng J 2:1–9. doi: 10.2174/187412070080201000110.2174/1874120700802010001 ↗
43. Pootawang P (2011) Solution plasma synthesis and characteristic for mesoporous silica and metal nanoparticles system. Dissertation, Nagoya University, Nagoya
44. Szegedi A, Popova M, Goshev I, Mihály J (2011) Effect of amine functionalization of spherical MCM-41 and SBA-15 on controlled drug release. J Solid State Chem 184:1201–1207. doi: 10.1016/j.jssc.2011.03.00510.1016/j.jssc.2011.03.005 ↗
45. Talebian N, Zare E (2014) Structure and antibacterial property of nano-SiO2 supported oxide ceramic. Ceram Int 40:281–287. doi: 10.1016/j.ceramint.2013.05.13510.1016/j.ceramint.2013.05.135 ↗
46. Jangra S, Girotra P, Chhokar V, Tomer VK, Sharma AK, Duhan S (2016) In vitro drug release kinetics studies of mesoporous SBA–15-azathioprine composite. J Porous Mater 23:679–688. doi: 10.1007/s10934-016-0123-110.1007/s10934-016-0123-1 ↗
47. Hillerstrom A, Andersson M, Samuelsson J, van Stam J (2014) Solvent strategies for loading and release in mesoporous silica. Colloids Interface Sci Commun 3:5–8. doi: 10.1016/j.colcom.2015.01.00110.1016/j.colcom.2015.01.001 ↗
48. Popova MD, Szegedi A, Kolev LN, Mihaly J, Tzankov BS, Momekov GT, Lambov NG, Yoncheva KP (2012) Carboxylic modified spherical mesoporous silicas as drug delivery carriers. Int J Pharm 436:778–785. doi: 10.1016/j.ijpharm.2012.07.06110.1016/j.ijpharm.2012.07.061 ↗
49. Sun L, Zhang X, Zheng C, Wu Z, Li CA (2013) pH gated, glucose-sensitive nanoparticle based on worm-like mesoporous silica for controlled insulin release. J Phys Chem B 117:3852–3860. doi: 10.1021/jp400442x10.1021/jp400442x ↗
50. Rehman F, Volpe PL, Airoldi C (2014) The applicability of ordered mesoporous SBA-15 and its hydrophobic glutaraldehyde-bridge derivative to improve ibuprofen-loading in releasing system. Colloids Surf B 119:82–89. doi: 10.1016/j.colsurfb.2014.03.04310.1016/j.colsurfb.2014.03.043 ↗
51. Choi SR, Jang DJ, Kim S, An S, OhE Lee J, Kim J (2014) Polymer-coated spherical mesoporous silica for pH-controlled delivery of insulin. J Mater Chem B 2:616–619. doi: 10.1039/C3TB21494J10.1039/C3TB21494J ↗
52. Mukhopadhyay P, Sarkar K, Chakraborty M, Bhattacharya S, Mishra R, Kundu PP (2013) Oral insulin delivery by self-assembled chitosan nanoparticles: in vitro and in vivo studies in diabetic animal model. Mater Sci Eng C 33:376–382. doi: 10.1016/j.msec.2012.09.00110.1016/j.msec.2012.09.001 ↗
53. Li Y, Jin M, Du Z, Liu X, Guo C, Li Y, Huang P, Sun Z (2011) Size-dependent cytotoxicity of amourphous silica nanoparticles in human hepatoma HepG2 cells. Toxicol In Vitro 25:1343–1352. doi: 10.1016/j.tiv.2011.05.00310.1016/j.tiv.2011.05.003 ↗
54. Lankoff A, Arabski M, Wegierek-Ciuk A, Kruszewski M, Lisowska H, Banasik-Nowak A, Rozga-Wijas K, Wojewodzka M, Slomkowski S (2013) Effect of surface modification of silica nanoparticles on toxicity and cellular uptake by human peripheral blood lynphocytes in vitro. Nanotoxicology 7:235–250. doi: 10.3109/17435390.2011.64979610.3109/17435390.2011.649796 ↗
55. Li HL, Zhang H, Huang H, Liu ZQ, Li YB, Yu H, An YH (2013) The effect of amino density on the attachment, migration, and differentiation of rat neural stem cells in vitro. Mol Cells 35:436–443. doi: 10.1007/s10059-013-0046-510.1007/s10059-013-0046-5 ↗
56. Hopper AP, Dugan JM, Gill AA, Fox OJ, May PW, Haycock JW, Claeyssens F (2014) Amine functionalized nanodiamond promotes cellular adhesion, proliferation and neurite outgrowth. Biomed Mater. doi: 10.1088/1748-6041/9/4/04500910.1088/1748-6041/9/4/045009 ↗
57. Singh SK, Singh MK, Kulkarni PP, Sonkart VK, Gracio JJA, Dash D (2012) Amine-modified graphene: thrombo-protective safer alternative to graphene oxide for biomedical applications. ACS Nano 6:2731–2740. doi: 10.1021/nn300172t10.1021/nn300172t ↗
58. Chen L, Mccrate JM, Lee JC, Li H (2011) The role of surface charge on the uptake and biocompatibility of hydroxyapatite nanoparticles with osteoblast cells. Nanotechnology. doi: 10.1088/0957-4484/22/10/10570810.1088/0957-4484/22/10/105708 ↗
59. Chung TH, Wu SH, Yao M, Lu CW, Lin YS, Hung Y, Mou CY, Chen YC, Huang DM (2007) The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells. Biomaterials 28:2959–2966. doi: 10.1016/j.biomaterials.2007.03.0010.1016/j.biomaterials.2007.03.00 ↗