Fabrication of strontium-substituted hydroxyapatite scaffolds using 3D printing for enhanced bone regeneration
Source abstract
Abstract
The use of porous three-dimensional (3D) bioceramic scaffolds to facilitate the regeneration of bone defects has attracted great attention because their structures closely mimic the natural extracellular matrix. 3D printing is a versatile method for the fabrication of 3D scaffolds. In this study, 3D strontium-substituted hydroxyapatite (Sr-HA) bioceramic scaffolds were prepared by simple precipitation and 3D printing method. The resulting scaffolds exhibited interconnected microporous structures of strands and a single-phase crystal due to HA, meaning that no changes in the phase composition and microstructure of the scaffolds with the Sr content were observed. However, their dissolution rate and biological performance were substantially influenced by changes in the Sr content of the scaffolds. The optimal Sr content in the Sr-HA scaffolds for enhanced proliferation and differentiation of cells were identified by comparing four compositions of the Sr-HA scaffolds. The results of in vitro bioactivity tests demonstrated that the Sr5-HA scaffold with 0.05 of Sr/(Ca + Sr) molar ratio promoted more rapid cell proliferation, osteogenic differentiation, and cellular mineralization compared with the other scaffolds. Therefore, Sr-HA scaffolds have the potential for application in bone regeneration as new bone graft substitutes.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Dental Implant Techniques and Outcomes · 3D Printing in Biomedical Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (26)
Show source-provided references
1. Wang L, Pathak JL, Liang D, Zhong N, Guan H, Wan M, Miao G, Li Z, Ge L (2020) Fabrication and characterization of strontium-hydroxyapatite/silk fibroin biocomposite nanospheres for bone-tissue engineering. Int J Biol Macromol 142:366–37510.1016/j.ijbiomac.2019.09.107 ↗
2. Prabha RD, Ding M, Bollen P, Ditzel N, Varma HK, Nair PD, Kassem M (2020) Strontium ion reinforced bioceramic scaffold for load bearing bone regeneration. Mater Sci Eng C 109:11042710.1016/j.msec.2019.110427 ↗
3. Meininger S, Moseke C, Spatz K, März E, Blum C, Ewald A, Vorndran E (2019) Effect of strontium substitution on the material properties and osteogenic potential of 3D powder printed magnesium phosphate scaffolds. Mater Sci Eng C 98:1145–115810.1016/j.msec.2019.01.053 ↗
4. Edwin N, Wilson SSP (2019) Strontium incorporated hydroxyapatite/hydrothermally reduced graphene oxide nanocomposite as a cytocompatibility materials. Ceram Int 45:5475–548510.1016/j.ceramint.2018.12.002 ↗
5. Zhang W, Shen Y, Pan H, Lin K, Liu X, Darvell BW, Lu WW, Chang J, Deng L, Wang D, Huang W (2011) Effects of strontium in modified biomaterials. Acta Biomater 7:800–80810.1016/j.actbio.2010.08.031 ↗
6. Shanmugam S, Gopal B (2014) Copper substituted hydroxyapatite and fluorapatite: synthesis, characterization and antimicrobial properties. Ceram Int 40:15655–1566210.1016/j.ceramint.2014.07.086 ↗
7. Nagyné-Kovács T, Studnicka L, Kincses A, Spengler G, Molnár M, Tolner M, Lukács IE, Szilágyi IM, Pokol G (2018) Synthesis and characterization of Sr and Mg-doped hydroxyapatite by a simple precipitation method. Ceram Int 44:22976–2298210.1016/j.ceramint.2018.09.096 ↗
8. Lee JH, Mandakhbayar N, El-Fiqi A, Kim HW (2017) Intracellular co-delivery of Sr ion and phenamil drug through mesoporous bioglass nanocarriers synergizes BMP signaling and tissue mineralization. Acta Biomater 60:93–10810.1016/j.actbio.2017.07.021 ↗
9. Peng S, Zhou G, Luk KDK, Cheung KMC, Li Z, Lam WM, Zhou Z, Lu WW (2009) Strontium promotes osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Cell Physiol Biochem 23:165–17410.1159/000204105 ↗
10. Zhang J, Wang M, Cha JM, Mantalaris A (2009) The incorporation of 70s bioactive glass to the osteogenic differentiation of murine embryonic stem cells in 3D bioreactors. J Tissue Eng Regen Med 3(1):63–7110.1002/term.135 ↗
11. Kavitha M, Subramanian R, Vinoth KS, Narayanan R, Venkatesh G, Esakkiraja N (2015) Optimization of process parameters for solution combustion synthesis of strontium substituted hydroxyapatite nanocrystals using design of experiments approach. Powder Technol 271:167–18110.1016/j.powtec.2014.10.046 ↗
12. Park SY, Kim KI, Park SP, Lee JH, Jung HS (2016) Aspartic acid-assisted synthesis of multifunctional strontium-substituted hydroxyapatite microspheres. Cryst Growth Des 16:4318–432610.1021/acs.cgd.6b00420 ↗
13. Liu D, Nie W, Li D, Wang W, Zheng L, Zhang J, Zhang J, Peng C, Mo X, He C (2019) 3D printed PCL/SrHA scaffold for enhanced bone regeneration. Chem Eng J 362:269–27910.1016/j.cej.2019.01.015 ↗
14. Zhang W, Feng C, Yang G, Li G, Ding X, Wang S, Dou Y, Zhang Z, Chang J, Wu C, Jiang X (2017) 3D-printed scaffolds with synergistic effect of hollow-pipe structure and bioactive ions for vascularized bone regeneration. Biomaterials 135:85–9510.1016/j.biomaterials.2017.05.005 ↗
15. Raucci MG, Giugliano D, Perez MAA, Ambrosio L (2015) Effects on growth and osteogenic differentiation of mesenchymal stem cells by the strontium-added sol–gel hydroxyapatite gel materials. J Mater Sci Mater Med 26:90–10110.1007/s10856-015-5436-0 ↗
16. Boanini E, Torricelli P, Sima F, Axente E, Fini M, Mihailescu IN, Bigi A (2018) Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response. J Inorg Biochem 183:1–810.1016/j.jinorgbio.2018.02.024 ↗
17. Lu X, Bao C, Xu HHK, Pan J, Hu J, Wang P, Luo E (2016) Osteoprotegerin gene-modified BMSCs with hydroxyapatite scaffold for treating critical-sized mandibular defects in ovariectomized osteoporotic rats. Acta Biomater 42:378–38810.1016/j.actbio.2016.06.019 ↗
18. Ni GX, Shu B, Huang G, Lu WW, Pan HB (2012) The effect of strontium incorporation into hydroxyapatites on their physical and biological properties. J Biomed Mater Res Part B Appl Biomater 100B:562–56810.1002/jbm.b.31986 ↗
19. Rajkumar M, Meenakshisundaram N, Rajendran V (2011) Development of nanocomposites based on hydroxyapatite/sodium alginate: synthesis and characterization. Mater Charact 62:469–47910.1016/j.matchar.2011.02.008 ↗
20. Padmanabhan SK, Haq EU, Licciulli A (2014) Rapid synthesis and characterization of silicon substituted nano hydroxyapatite using microwave irradiation. Curr Appl Phys 14:87–9210.1016/j.cap.2013.08.022 ↗
21. Jiang J, Han G, Zheng X, Chen G, Zhu P (2019) Characterization and biocompatibility study of hydroxyapatite coating on the surface of titanium alloy. Surf Coat Technol 375:645–65110.1016/j.surfcoat.2019.07.067 ↗
22. Zhu H, Guo D, Zang H, Hanaor DAH, Yu S, Schmidt F, Xu K (2020) Enhancement of hydroxyapatite dissolution through structure modification by Krypton ion irradiation. J Mater Sci Technol 38:148–15810.1016/j.jmst.2019.03.048 ↗
23. Zhang E, Zou C, Yu G (2009) Surface microstructure and cell biocompatibility of silicon-substituted hydroxyapatite coating on titanium substrate prepared by a biomimetic process. Mater Sci Eng C 29:298–30510.1016/j.msec.2008.07.001 ↗
24. Prabha RD, Nair BP, Ditzel N, Kjems J, Nair PD, Kassem M (2019) Strontium functionalized scaffold for bone tissue engineering. Mater Sci Eng C 94:509–51510.1016/j.msec.2018.09.054 ↗
25. Naruphontjirakul P, Porter AE, Jones JR (2018) In vitro osteogenesis by intracellular uptake of strontium containing bioactive glass nanoparticles. Acta Biomater 66:67–8010.1016/j.actbio.2017.11.008 ↗
26. Humphrey EL, Williams JHH, Davie MWJ, Marshall MJ (2006) Effects of dissociate glucocorticoids on OPG and RANKL in osteoblastic cells. Bone 38:652–66110.1016/j.bone.2005.10.004 ↗