Ultrasensitive aptamer-based biosensor for acetamiprid using tetrahedral DNA nanostructures
Source abstract
Abstract
An electrochemical tetrahedral APT biosensor (Au-TAN biosensor) was successfully constructed, which had a stable structure and excellent performance using tetrahedral DNA nanomaterials (TDN) and Au nanoparticles (AuNPs) for the detection of acetamiprid. Specifically, we designed a partial paired complementary DNA (cDNA) of acetamiprid aptamer and inserted the cDNA together with the acetamiprid aptamer into four single-stranded DNA sequences forming each side of the TDN, modifying AuNPs to increase the current signal intensity, to assemble the Au-TAN. This proposed aptasensor was compared with ssAPT aptasensor and tetrahedral APT nanostructures (TAN) aptasensor, respectively. The results showed that the performance of the designed aptasensor was better than those of the above two aptasensors. The detection limit of the aptasensor was calculated to be 0.077 pM (S/N = 3), which was far lower than that of other methods that we compared. Under optimized conditions, the proposed aptasensor showed a wide linear detection range from 0.1 pM to 10 nM. The Au-TAN biosensor opens up new ideas for the construction of aptasensors and provides a new method for detection of small molecular substances, such as pesticides residues.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · SARS-CoV-2 detection and testing
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (52)
Show source-provided references
1. Fan K-M, Kang W-K, Qu S-F, Li L, Qu B-H, Lu L-H (2019) A label-free and enzyme-free fluorescent aptasensor for sensitive detection of acetamiprid based on AT-rich dsDNA-templated copper nanoparticles. Talanta 197:64510.1016/j.talanta.2019.01.069 ↗
2. Kiriyama K, Itazu Y, Kagabu S, Nishimura K, Pestic J (2004) Insecticidal and neuroblocking activities of acetamiprid and related compounds. Science 28:8
3. Morgan AR (2007) Acetamiprid: novel neonicotinoid systemic insecticide. Asp Appl Biol 83:47
4. Mohan C, Kumar Y, Madan J, Saxena N (2010) Multiresidue analysis of neonicotinoids by solid-phase extraction technique using high-performance liquid chromatography. Environ Monit Assess 165:57310.1007/s10661-009-0968-8 ↗
5. Madianos L, Tsekenis G, Skotadis E, Patsiouras L, Tsoukalas D (2018) A highly sensitive impedimetric aptasensor for the selective detection of acetamiprid and atrazine based on microwires formed by platinum nanoparticles. Biosens Bioelectron 101:26810.1016/j.bios.2017.10.034 ↗
6. Qin X-S, Du G, Jin H, Li X, Xiao Y-H (2011) Determination of acetamiprid by a colorimetric method based on the aggregation of gold nanoparticles. Microchim Acta 173:32310.1007/s00604-011-0562-y ↗
7. Imamura T, Yanagawa Y, Nishikawa K, Matsumoto N, Sakamoto T (2010) Two cases of acute poisoning with acetamiprid in humans. Clin Toxicol 48:85110.3109/15563650.2010.517207 ↗
8. Qi Y-Y, Xiu F-R, Zheng M-F, Li B-X (2016) A simple and rapid chemiluminescence aptasensor for acetamiprid in contaminated samples: Sensitivity, selectivity and mechanism. Biosens Bioelectron 83:24310.1016/j.bios.2016.04.074 ↗
9. Ragas AMJ, Oldenkamp R, Preeker NL, Wernicke J, Schlink U (2011) Cumulative risk assessment of chemical exposures in urban environments. Environ Int 37:87210.1016/j.envint.2011.02.015 ↗
10. Kaur RP, Gupta V, Christopher AF, Bansal P (2015) Potential pathways of pesticide action on erectile function-a contributory factor in male infertility. Asia Pac J Reprod 4:32210.1016/j.apjr.2015.07.012 ↗
11. Vichapong J, Burakham R, Srijaranai S (2016) Alternative liquid-liquid microextraction as cleanup for determination of neonicotinoid pesticides prior HPLC analysis. Chromatographia 79:28510.1007/s10337-016-3022-3 ↗
12. Muccio AD, Fidente P, Barbini DA, Dommarco R, Seccia S, Morrica P (2006) Application of solid-phase extraction and liquid chromatography-mass spectrometry to the determination of neonicotinoid pesticide residues in fruit and vegetables. J Chromatogr A 1108:110.1016/j.chroma.2005.12.111 ↗
13. Park JY, Choi JH, Kim BM, Park JH, Cho SK, Ghafar MW, Abd El-Atyd AM, Shim JH (2011) Determination of acetamiprid residues in zucchini grown under greenhouse conditions: application to behavioral dynamics. Biomed Chromatogr 25:13610.1002/bmc.1529 ↗
14. Ferrer I, Thurman EM, Fernandez-Alba AR (2005) Quantitation and accurate mass analysis of pesticides in vegetables by LC/TOF-MS. Anal Chem 77:281810.1021/ac048458x ↗
15. López-fernández O, Rialotero R, Simalgándara J (2015) High-throughput hplc–ms/ms determination of the persistence of neonicotinoid insecticide residues of regulatory interest in dietary bee pollen. Anal Bioanal Chem 407:710110.1007/s00216-015-8870-4 ↗
16. Radisic M, Grujic S, Vasiljevic T, Lausevic M (2009) Determination of selected pesticides in fruit juices by matrix solid-phase dispersion and liquid chromatography-tandem mass spectrometry. Food Chem 113:71210.1016/j.foodchem.2008.07.103 ↗
17. Zhang X-A, Mobley N, Zhang J-G, Zheng X-M, Lu L, Ragin O, Smith CJ, Agric J (2010) Analysis of agricultural residues on tea using d-SPE sample preparation with GC-NCI-MS and UHPLC-MS/MS. Food Chem 58:1155310.1021/jf102476m ↗
18. Wanatabe S, Ito S, Kamata Y, Omoda N, Yamazaki T, Munakata H, Kaneko T, Yuasa Y (2001) Development of competitive enzyme-linked immunosorbent assays (ELISAs) based on monoclonal antibodies for chloronicotinoid insecticides imidacloprid and acetamiprid. Anal Chim Acta 427:21110.1016/S0003-2670(00)01126-0 ↗
19. Kocaman A, Topaktas M (2010) Genotoxic effects of a particular mixture of acetamiprid and alpha-cypermethrin on chromosome aberration, sister chromatid exchange, and micronucleus formation in human peripheral blood lymphocytes. Environ Toxicol 25:15710.1002/tox.20485 ↗
20. Bahreyni A, Yazdian-Robati R, Ramezani M, Abnous K, Taghdisi SM (2018) Fluorometric aptasensing of the neonicotinoid insecticide acetamiprid by using multiple complementary strands and gold nanoparticles. Microchim Acta 185:27210.1007/s00604-018-2805-7 ↗
21. Verma N, Bhardwaj A (2015) Appl biosensor technology for pesticides-a review. Biochem Biotechnol 175:309310.1007/s12010-015-1489-2 ↗
22. Akyüz D, Koca A (2019) An electrochemical sensor for the detection of pesticides based on the hybrid of manganese phthalocyanine and polyaniline. Sens Actuators B 283:848.10.1016/j.snb.2018.11.155 ↗
23. Bang GS, Cho S, Lee N, Lee BR, Kim JH, Kim BG (2013) Rational design of modular allosteric aptamer sensor for label-free protein detection. Biosens Bioelectron 39:4410.1016/j.bios.2012.06.038 ↗
24. Cheng S-T, Liu H-M, Zhang H, Chu G-L, Guo Y-M, Sun X (2020) Ultrasensitive electrochemiluminescence aptasensor for kanamycin detection based on silver nanoparticle-catalyzed chemiluminescent reaction between luminol and hydrogen peroxide. Sens Actuators B 304:12736710.1016/j.snb.2019.127367 ↗
25. Fu J-Y, Dong H-W, Zhao Q-X, Cheng S-T, Guo Y-M, Sun X (2020) Fabrication of refreshable aptasensor based on hydrophobic screen-printed carbon electrode interface. Sci Total Environ 712:13641010.1016/j.scitotenv.2019.136410 ↗
26. Fu J-Y, An X-S, Yao Y, Guo Y-M, Sun X (2019) Electrochemical aptasensor based on one step co-electrodeposition of aptamer and GO-CuNPs nanocomposite for organophosphorus pesticide detection. Sens Actuators B, 503–509.10.1016/j.snb.2019.02.057 ↗
27. Yao Y, Wang G-X, Chu G-L, An X-S, Guo Y-M, Sun X (2019) Development of a novel biosensor based on gold nanocages/graphene oxide-chitosan modification acetylcholinesterase for organophosphorus pesticides detection. New J Chem 43:13816–1382610.1039/C9NJ02556A ↗
28. Alsager OA, Kumar S, Willmott GR, McNatty KP, Hodgkiss JM (2014) Small molecule detection in solution via the size contraction response of aptamer functionalized nanoparticles. Biosens Bioelectron 57:26210.1016/j.bios.2014.02.004 ↗
29. Bai X-J, Hou H, Zhang B-L, Tang J-L (2014) Label-free detection of kanamycin using aptamer-based cantilever array sensor. Biosens Bioelectron 56:11210.1016/j.bios.2013.12.068 ↗
30. Hassani S, Momtaz S, Vakhshiteh F, Maghsoudi AS, Ganjali MR, Norouzi P, Abdollahi M (2017) Biosensors and their applications in detection of organophosphorus pesticides in the environment. Arch Toxicol 91:10910.1007/s00204-016-1875-8 ↗
31. Sassolas A, Prietosimón B, Marty JL (2012) Biosensors for pesticide detection: new trends. Am J Anal Chem 3:21010.4236/ajac.2012.33030 ↗
32. He J, Liu Y, Fan M-T, Liu X-J (2011) Isolation and identification of the DNA aptamer target to acetamiprid. J Agric Food Chem 59:158210.1021/jf104189g ↗
33. Shi X, Sun J, Yao Y, Liu H-M, Huang J-C, Guo Y-M, Sun X (2020) Novel electrochemical aptasensor with dual signal amplification strategy for detection of acetamiprid. Sci Total Environ 705:13590510.1016/j.scitotenv.2019.135905 ↗
34. Qi Y-Y, Chen Y-T, Xiu F-R, Hou J-X (2020) An aptamer-based colorimetric sensing of acetamiprid in environmental samples: convenience, sensitivity and practicability. Sensor Actuat B Chem 304:12735910.1016/j.snb.2019.127359 ↗
35. Qi Y-Y, Ma J-X, Chen X-D, Xiu F-R, Chen Y-T, Lu Y-W (2020) Practical aptamer-based assay of heavy metal mercury ion in contaminated environmental samples: convenience and sensitivity. Anal Bioanal Chem 412:439–44810.1007/s00216-019-02253-8 ↗
36. Padmanabhan K, Padmanabhan KP, Ferrara JD, Sadler JE, Tulinsky A (1993) The structure of alpha-thrombin inhibited by a 15-mer single-stranded DNA aptamer. J Biol Chem 268:1765110.1016/S0021-9258(17)46749-4 ↗
37. Sharma J, Chhabra R, Cheng A, Brownell J, Liu Y, Yan H (2009) Control of self-assembly of DNA tubules through integration of gold nanoparticles. Science 323:11210.1126/science.1165831 ↗
38. Ge Z-L, Su Z-M, Simmons CR, Li J, Jiang S-X, Li W, Yang Y, Liu Y, Chi W, Fan C-H, Yan H (2019) Redox Engineering of cytochrome c using DNA nanostructure-based charged encapsulation and spatial control. ACS Appl Mater Interfaces 11:1387410.1021/acsami.8b07101 ↗
39. Li C, Hu X-L, Lu J-Y, Mao X-X, Xiang Y, Shu Y-Q, Li G-X (2018) Design of DNA nanostructure-based interfacial probes for the electrochemical detection of nucleic acids directly in whole blood. Chem Sci 9:97910.1039/C7SC04663D ↗
40. Kim KR, Kim DR, Lee T, Yhee JY, Kim BS, Kwon IC, Ahn DR (2013) Drug delivery by a self-assembled DNA tetrahedron for overcoming drug resistance in breast cancer cells. Chem Commun 49:201010.1039/c3cc38693g ↗
41. Yang F, Li Q, Wang L-H, Zhang G-J, Fan C-H (2018) Framework-nucleic-acid-enabled biosensor development. ACS Sens 3:90310.1021/acssensors.8b00257 ↗
42. Iqbala M, Usanasea G, Oulmib K, Aberkane F, Bendaikha T, Fessi H, Zine N, Agusti G, Errachid A, Elaissari A (2016) Preparation of gold nanoparticles and determination of their particles size via different methods. Mater Res Bull 79:9710.1016/j.materresbull.2015.12.026 ↗
43. Yan W-J, Xu L-G, Xu C-L, Ma W, Kuang H, Wang L-B, Kotov NA (2012) Self-assembly of chiral nanoparticle pyramids with strong R/S optical activity. J Am Chem Soc 134:1511410.1021/ja3066336 ↗
44. Storhoff JJ, Elghanian R, Mucic RC, Mirkin CA, Letsinge RL (1998) One-Pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. J Am Chem Soc 120:195910.1021/ja972332i ↗
45. Yi J-L, Liu Z, Liu J-H, Liu H-B, Xia F-Q, Tian D, Zhou C-L (2020) A label-free electrochemical aptasensor based on 3D porous CS/rGO/GCE for acetamiprid residue detection. Biosens Bioelectron 148:11182710.1016/j.bios.2019.111827 ↗
46. Wang H, Li H, Huang Y, Xiong M-H, Wang F, Li C (2019) A label-free electrochemical biosensor for highly sensitive detection of gliotoxin based on DNA nanostructure/MXene nanocomplexes. Biosens Bioelectron 142:11153110.1016/j.bios.2019.111531 ↗
47. Yang Y-C, Huang Y, Li C (2019) A reusable electrochemical sensor for one-step biosensing in complex media using triplex-forming oligonucleotide coupled DNA nanostructure. Anal Chim Acta 1055:90–9710.1016/j.aca.2018.12.031 ↗
48. Feng Q-M, Wang M-Y, Chen Q, Wang P (2018) Direct electrochemical detection of guanosine-5’-monophosphate at choline monolayer supported and gold nanocages functionalized carbon nanotubes sensing interface. Sens Actuators B 274:343.
49. Feng Q-M, Zhou Z, Li M-X, Zhao W, Xu J-J, Chen H-Y (2017) DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor. Biosens Bioelectron 90:25110.1016/j.bios.2016.11.060 ↗
50. Ding J-W, Zhang H-X, Jia F-L, Qin W, Du D (2014) Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design. Sens Actuators B 199:284.10.1016/j.snb.2014.04.012 ↗
51. Li Z-H, Zhao B, Wang D-F, Wen Y-L, Liu G, Dong H-Q, Song S-P, Fan C-H (2014) DNA Nanostructure-Based Universal Microarray Platform for High Efficiency Multiplex Bioanalysis in Biofluids. ACS Appl Mater Interfaces 6:1794410.1021/am5047735 ↗
52. Jiao Y-C, Hou W-J, Fu J-Y, Guo Y-M, Sun X, Wang X-Y, Zhao J (2017) A nanostructured electrochemical aptasensor for highly sensitive detection of chlorpyrifos. Sens Actuators B 243:1164.10.1016/j.snb.2016.12.106 ↗