Negatively charged gold nanoparticles as an intrinsic peroxidase mimic and their applications in the oxidation of dopamine
Source abstract
Abstract
Artificial inorganic peroxidase is of great interest due to its intrinsic advantages over natural counterpart. Negatively charged gold nanoparticles (AuNPs) were discovered to function like a peroxidase in the present study. Two AuNPs in different size were prepared and characterized by TEM, and assayed for peroxidase activity. Its catalytic activity was found to follow Michaelis–Menten kinetics. The negative surface charge notably improves the affinity toward a substrate TMB, proved by the determined kinetic parameters. The particles expressed optimal catalytic activity under mildly acidic environment and resistance to elevated temperature and increased concentration of sodium azide. The origin of the activity was investigated tentatively. Hydrogen peroxide-treated AuNPs exhibited an enhanced activity. EDTA temporarily blocked the activity partially, while thiol groups permanently blocked the activity completely. Tests imply that it is the surface Au+ that provides the activity. The successful oxidation of dopamine, as an instance, under the action of AuNPs as a peroxidase was conducted. These studies would lead to a wide range of potential applications.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced Nanomaterials in Catalysis · Electrochemical sensors and biosensors · Nanocluster Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (29)
Show source-provided references
1. Saha K, Agasti SS, Kim C et al (2012) Gold nanoparticles in chemical and biological sensing. Chem Rev 112:2379–273910.1021/cr2001178 ↗
2. Louis C, Pluchery O (2012) Gold nanoparticles for physics, chemistry, and biology. Imperial College Press, London10.1142/p815 ↗
3. Sardar R, Funston AM, Mulvaney P et al (2009) Gold nanoparticles: past, present, and future. Langmuir 25:13840–1385110.1021/la9019475 ↗
4. Bunz UHF, Rotello VM (2010) Gold Nanoparticle–fluorophore complexes: sensitive and siscerning “noses” for biosystems sensing. Angew Chem Int Ed 49:3268–327910.1002/anie.200906928 ↗
5. Liu J, Guan Z, Lv Z et al (2014) Improving sensitivity of gold nanoparticle based fluorescence quenching and colorimetric aptasensor by using water resuspended gold nanoparticles. Biosens Bioelectron 52:265–27010.1016/j.bios.2013.08.059 ↗
6. Jain KK (2012) The Handbook of Nanomedicine. Springer, New York Heidelberg Dordrecht London10.1007/978-1-61779-983-9 ↗
7. Jia F, Liu X, Li L et al (2013) Multifunctional nanoparticles for targeted delivery of immune activating and cancer therapeutic agents. J Control Release 172:1020–103410.1016/j.jconrel.2013.10.012 ↗
8. Mieszawska AJ, Mulder WJM, Fayad ZA et al (2013) Multifunctional gold nanoparticles for diagnosis and therapy of disease. Mol Pharm 10:831–84710.1021/mp3005885 ↗
9. Ansar SM, Perera GS, Ameer FS et al (2013) Desulfurization of mercaptobenzimidazole and thioguanine on gold nanoparticles using sodium borohydride in water at room temperature. J Phys Chem C 137:13722–1372910.1021/jp403932w ↗
10. Liu X, He L, Liu Y-M et al (2014) Supported gold catalysis: from small molecule activation to green chemical synthesis. Acc Chem Res 47:793–80410.1021/ar400165j ↗
11. Biswas S, Sengupta S, Roy Chowdhury S et al (2014) CXCL13-CXCR5 co-expression regulates epithelial to mesenchymal transition of breast cancer cells during lymph node metastasis. Breast Cancer Res Treat 143:265–27610.1007/s10549-013-2811-8 ↗
12. Li Y, Schluesener HJ, Xu S (2010) Gold nanoparticle-based biosensors. Gold Bulletin 43:29–4110.1007/BF03214964 ↗
13. Lan D, Li B, Zhang Z (2008) Chemiluminescence flow biosensor for glucose based on gold nanoparticle-enhanced activities of glucose oxidase and horseradish peroxidase. Biosen Bioelectron 24:940–944
14. Gao L, Zhuang J, Nie L et al (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotech 2:577–58310.1038/nnano.2007.260 ↗
15. Yu F, Huang Y, Cole AJ et al (2009) The artificial peroxidase activity of magnetic iron oxide nanoparticles and its application to glucose detection. Biomaterials 30:4716–472210.1016/j.biomaterials.2009.05.005 ↗
16. Liu Y, Yu F (2011) Substrate-specific modifications on magnetic iron oxide nanoparticles as an artificial peroxidase for improving sensitivity in glucose detection. Nanotechnology 22:145704–14571110.1088/0957-4484/22/14/145704 ↗
17. Zuo X, Peng C, Huang Q et al (2009) Design of a carbon nanotube/magnetic nanoparticle-based peroxidase-like nanocomplex and its application for highly efficient catalytic oxidation of phenols. Nano Res 2:617–62310.1007/s12274-009-9062-3 ↗
18. Jv Y, Li B, Cao R (2010) Positively-charged gold nanoparticles as peroxidiase mimic and their application in hydrogen peroxide and glucose detection. Chem Commun 46:8017–801910.1039/c0cc02698k ↗
19. Brown KR, Fox AP, Natan MJ (1996) Morphology-dependent electrochemistry of cytochrome c at Au Colloid-Modified SnO2 Electrodes. J Am Chem Soc 118:1154–115710.1021/ja952951w ↗
20. Storhoff JJ, Lazarides AA, Mucic RC et al (2000) What controls the optical properties of DNA-linked gold nanoparticle assemblies? J Am Chem Soc 122:4640–465010.1021/ja993825l ↗
21. Park JW, Shumaker-Parry JS (2014) Structural study of citrate layers on gold nanoparticles: role of intermolecular interactions in stabilizing nanoparticles. J Am Chem Soc 136:1907–192110.1021/ja4097384 ↗
22. Bally RW, Gribnau TCJ (1989) Some aspects of the chromogen 3,3′,5,5′-tetramethylbenzidine as hydrogen donor in a horseradish peroxidase assay. J Clin Chem Clin Biochem 27:791–796
23. Karasyova EI, Losev YuP, Metelitza DI (2002) Peroxidase-catalyzed oxidation of 3, 3′, 5, 5′-tetramethylbenzidine in the presence of 2,4-dinitrosoresorcinol and polydisulfide derivatives of resorcinol and 2,4-dinitrosoresorcinol. Russ J Bioorg Chem 28:128–13510.1023/A:1015069424251 ↗
24. Napolitano A, Crescenzi O, Pezzella A et al (1995) Generation of the neurotoxin 6-hydroxydopamine by peroxidase/H2O2 oxidation of dopamine. J Med Chem 38:917–92210.1021/jm00006a010 ↗
25. Sandroff CJ, Herschbach DR (1985) Kinetics of displacement and charge transfer reactions probed by SERS: evidence for distinct donor and acceptor sites on colloidal gold surfaces. Langmuir 1:131–13510.1021/la00061a022 ↗
26. Cortie MB, Lingen EVD (2002) Catalytic gold nano-particles. Mater Forum 26:1–14
27. Badia A, Cuccia L, Demers L et al (1997) Structure and dynamics in alkanethiolate monolayers self-assembled on gold nanoparticles: a DSC, FT-IR, and deuterium NMR study. J Am Chem Soc 119:2682–269210.1021/ja963571t ↗
28. Pandey P, Singh SP, Arya SK et al (2007) Application of thiolated gold nanoparticles for the enhancement of glucose oxidase activity. Langmuir 23:3333–333710.1021/la062901c ↗
29. Mackey MA, Saira F, Mahmoud MA et al (2013) Inducing cancer cell death by targeting its nucleus: solid gold nanospheres versus hollow gold nanocages. Bioconjug Chem 24:897–90610.1021/bc300592d ↗