Fabrication and Comprehensive Characterization of Biomimetic Extracellular Matrix Electrospun Scaffold for Vascular Tissue Engineering Applications
Source abstract
Abstract
The collagen–hyaluronic acid oligosaccharides (collagen–HA) complex was obtained by covalent attachment using EDC/NHS. The cross-linked product collagen–HA had been confirmed by Fourier transform infrared spectra (FTIR) and the sulfuric acid–carbazole colorimetry, and factors that affect the cross-linking process were investigated. Electrospinning of collagen–HA complex in hexafluoroisopropanol was investigated for the biomimetic nanostructure and biocompatible vascular tissue-engineered inner-layer scaffold. The morphology of the electrospun collagen–HA nanofibers with a series of concentrations was observed by scanning electron microscopy. The thermal behavior and chemical properties of the fabricated scaffolds were characterized using dynamic scanning calorimetry and FTIR after being stabilized by glutaraldehyde vapor. The cell attachment, morphology and proliferation of porcine iliac artery endothelial cells (PIECs) on the electrospun scaffolds were also evaluated. The results indicate that the biomimetic matrix collagen–HA electrospun nanofibrous scaffold could promote PIECs proliferation and may be a better candidate with endothelial potential for the vascular tissue-engineered inner-layer scaffold.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Tissue Engineering and Regenerative Medicine · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (34)
Show source-provided references
1. Horakova J, Mikes P, Saman A, Svarcova T, Jencova V, Suchy T, Heczkova B, Jakubkova S, Jirousova J, Prochazkova R (2017) Comprehensive assessment of electrospun scaffolds hemocompatibility. Mater Sci Eng C Mater Biol Appl 82:330–33510.1016/j.msec.2017.05.011 ↗
2. Badylak SF, Freytes DO, Gilbert TW (2015) Extracellular matrix as a biological scaffold material: structure and function. Acta Biomater 23(1):S17–S2610.1016/j.actbio.2015.07.016 ↗
3. Srouji S, Kizhner T, Suss-Tobi E, Livne E, Zussman E (2008) 3-D Nanofibrous electrospun multilayered construct is an alternative ECM mimicking scaffold. J Mater Sci Mater Med 19(3):1249–1255. https://doi.org/10.1007/s10856-007-3218-z10.1007/s10856-007-3218-z ↗
4. Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S (2003) A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 63(15):2223–225310.1016/S0266-3538(03)00178-7 ↗
5. Prabu P, Dharmaraj N, Aryal S, Lee BM, Ramesh V, Kim HY (2010) Preparation and drug release activity of scaffolds containing collagen and poly(caprolactone). J Biomed Mater Res Part A 79A(1):153–15810.1002/jbm.a.30715 ↗
6. Loh XJ, Peh P, Liao S, Sng C, Li J (2010) Controlled drug release from biodegradable thermoresponsive physical hydrogel nanofibers. J Controll Release 143(2):175–18210.1016/j.jconrel.2009.12.030 ↗
7. Prabu P, Dharmaraj N, Aryal S, Lee BM, Ramesh V, Kim HY (2010) J Biomed Mater Res Part A 79A(1):153–15810.1002/jbm.a.30715 ↗
8. Ma Z, Kotaki M, Yong T, He W, Ramakrishna S (2005) Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering. Biomaterials 26(15):2527–253610.1016/j.biomaterials.2004.07.026 ↗
9. Ren X, Feng Y, Guo J, Wang H, Li Q, Yang J, Hao X, Lv J, Ma N, Li W (2015) Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications. Chem Soc Rev 44(15):5680–574210.1039/C4CS00483C ↗
10. Avci-Adali M, Ziemer G, Wendel HP (2010) Induction of EPC homing on biofunctionalized vascular grafts for rapid in vivo self-endothelialization: a review of current strategies. Biotechnol Adv 28(1):119–12910.1016/j.biotechadv.2009.10.005 ↗
11. Chuang TW, Masters KS (2009) Regulation of polyurethane hemocompatibility and endothelialization by tethered hyaluronic acid oligosaccharides. Biomaterials 30(29):5341–535110.1016/j.biomaterials.2009.06.029 ↗
12. Morra M (2005) Engineering of biomaterials surfaces by hyaluronan. Biomacromol 6(3):1205–122310.1021/bm049346i ↗
13. Burdick JA, Prestwich GD (2011) Hyaluronic acid hydrogels for biomedical applications. Adv Mater 23(12):H41–H5610.1002/adma.201003963 ↗
14. Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem 4(4):330–334. https://doi.org/10.1016/0003-2697(62)90095-710.1016/0003-2697(62)90095-7 ↗
15. Chen ZG, Wang PW, Wei B, Mo XM, Cui FZ (2010) Electrospun collagen–chitosan nanofiber: a biomimetic extracellular matrix for endothelial cell and smooth muscle cell. Acta Biomater 6(2):372–38210.1016/j.actbio.2009.07.024 ↗
16. Neamnark A, Sanchavanakit N, Pavasant P, Rujiravanitaa R (2008) In vitro biocompatibility of electrospun hexanoyl chitosan fibrous scaffolds towards human keratinocytes and fibroblasts. Eur Polym J 44(7):2060–206710.1016/j.eurpolymj.2008.04.016 ↗
17. Garside P, Wyeth P, Lahlil S (2005) Characterization of historic silk by polarized attenuated total reflectance Fourier transform infrared spectroscopy for informed conservation. Appl Spectrosc 59(10):1242–124710.1366/000370205774430855 ↗
18. Chung TW, Chang YL (2010) Silk fibroin/chitosan–hyaluronic acid versus silk fibroin scaffolds for tissue engineering: promoting cell proliferations in vitro. J Mater Sci Mater Med 21(4):1343–1351. https://doi.org/10.1007/s10856-009-3876-010.1007/s10856-009-3876-0 ↗
19. Ramakrishna S, Fujihara K, Teo WE, Lim TC, Ma Z (2008) An introduction to electrospinning and nanofibers. J Eng Fibers Fabr 3(2):46–47
20. Chen Z, Mo X, Qing F (2007) Electrospinning of collagen–chitosan complex. Mater Lett 61(16):3490–349410.1016/j.matlet.2006.11.104 ↗
21. Zeng J, Chen XS, Xu XY, Liang QZ, Bian XC, Yang LX, Jing XB (2010) Ultrafine fibers electrospun from biodegradable polymers. J Appl Polym Sci 89(4):1085–109210.1002/app.12260 ↗
22. Haider A, Haider S, Kang IK (2015) A comprehensive review summarizing the effect of electrospinning parameters and potential applications of nanofibers in biomedical and biotechnology. Arab J Chem 11(8):1165–118810.1016/j.arabjc.2015.11.015 ↗
23. Subbiah T, Bhat GS, Tock RW, Parameswaran S, Ramkumar SS (2010) Electrospinning of nanofibers. J Appl Polym Sci 96(2):557–56910.1002/app.21481 ↗
24. Chen ZG, Wang PW, Wei B, Mo XM, Cui FZ (2010) Electrospun collagen–chitosan nanofiber: a biomimetic extracellular matrix for endothelial cell and smooth muscle cell. Acta Biomater 6(2):372–38210.1016/j.actbio.2009.07.024 ↗
25. Wang XH, Li DP, Wang WJ, Feng QL, Cui FZ, Xu YX, Song XH, Mark VDW (2003) Crosslinked collagen/chitosan matrix for artificial livers. Biomaterials 24(19):3213–322010.1016/S0142-9612(03)00170-4 ↗
26. Seifu DG, Purnama A, Mequanint K, Mantovani D (2013) Small-diameter vascular tissue engineering. Nat Rev Cardiol 10(7):410–42110.1038/nrcardio.2013.77 ↗
27. Rashid S, Fuller B, Hamilton G, Seifalian A (2008) Tissue engineering of a hybrid bypass graft for coronary and lower limb bypass surgery. FASEB J 22(6):2084–208910.1096/fj.07-096586 ↗
28. Abbott WM, Callow A, Moore W, Rutherford R, Veith F, Weinberg S (1993) Evaluation and performance standards for arterial prostheses. J Vasc Surg 17(4):746–75610.1016/0741-5214(93)90120-B ↗
29. Bennion RS, Williams RA, Stabile BE, Fox MA, Owens ML, Wilson SE (1985) Patency of autogenous saphenous vein versus polytetrafluoroethylene grafts in femoropopliteal by pass for advanced ischemia of the extremity. Surg Gynecol Obstetr 160(3):239–242
30. Deutsch M, Meinhart J, Fischlein T, Preiss P, Zilla P (1999) Clinical autologous in vitro endothelialization of infrainguinal ePTFE grafts in 100 patients: a 9-year experience. Surgery 126(5):847–85510.1016/S0039-6060(99)70025-5 ↗
31. West DC, Hampson IN, Arnold F, Kumar S (1985) Angiogenesis induced by degradation products of hyaluronic acid. Science 228(4705):1324–132610.1126/science.2408340 ↗
32. Goh YF, Shakir I, Hussain R (2013) Electrospun fibers for tissue engineering, drug delivery, and wound dressing. J Mater Sci 48(8):3027–3054. https://doi.org/10.1007/s10853-013-7145-810.1007/s10853-013-7145-8 ↗
33. Salacinski HJ, Goldner S, Giudiceandrea A, Hamilton G, Seifalian AM, Edwards A, Carson RJ (2001) The mechanical behavior of vascular grafts: a review. J Biomater Appl 15(3):241–27810.1106/NA5T-J57A-JTDD-FD04 ↗
34. Qiao Y, Liu X, Fu G, He Z, Hou C, Li Y, Zhang Q, Yan H, Wang H (2018) An ordered electrospun polycaprolactone–collagen–silk fibroin scaffold for hepatocyte culture. J Mater Sci 53(3):1623–1633. https://doi.org/10.1007/s10853-017-1670-910.1007/s10853-017-1670-9 ↗