Influence of core-shell polydopamine-barium titanate doping on the osseointegration and cytotoxicity of the polypyrrole coatings applied by ECD on Nitinol orthopedic implants

In the current study, a thin polydopamine (PDA) layer of around 5 nm was created on the barium titanate (BT) nanoparticles through self-polymerization of dopamine. The produced modified PDA/BT core-shell nanoparticles , termed PBT, were co-electrodeposited with polypyrrole (PPy) onto Nitinol (NiTi) alloy substrates in concentrations ranging from 1 to 10% by weight to produce PPy/PBT composite coatings . The incorporation of these particles changed PPy's cauliflower morphology into a rougher but more compact nodular morphology. According to the atomic force microscopy results, the average surface roughness of PPy coating increased from 44.2 nm up to 80.2 nm by the co-deposition of PBT. Aside from variations in morphology and roughness values, all of the PPy and PPy/PBT coatings demonstrated hydrophilicity . Nonetheless, while the majority of the composite coatings had higher water contact angle (WCA) than the unreinforced PPy, the PPy/10PBT composite coating had the lowest WCA of all samples, measuring 56.9°. The piezoelectric responses of the coatings were also examined, and the best specimen was the PPy/5PBT composite coating with an enhanced output voltage of approximately 400 mV. Finally, MC3T3 mouse osteoblast cells were used for in vitro cell culture evaluations. While healthy cells were evenly distributed on the surface of all coatings after 3 days, indicating biocompatibility, cell density on composite coatings was higher than on unreinforced PPy coating. According to the MTT experiment, the vitality of MC3T3 osteoblast cells on the surface of PPy/5PBT composite coating was more than 30% greater than that on the surface of PPy coating.