journal article·2025-06-14

Surface modification by nanosecond laser texturing imparts anti-adhesive characteristics to implant materials

Neelesh Sirdeshmukh, Ganesh Dongre, Snehal Kulkarni, Jyutika Rajwade

Journal of Materials Research· Volume 40· Issue 11
Publisher page

Abstract

The multifactorial investigation of nanosecond laser-textured Ti6Al4V for anti-adhesive characteristics is inadequate. The present work investigated the adhesion ofS. aureusandE. colion a nanosecond Nd:YAG laser (1064 nm) textured Ti6Al4V surfaces. Cross-grooves with 2 µm microchannels with increased Ti2p3/2Ti4+and Ti2p1/2Ti4+concentrations exhibit 99.3% and 82.2% reduction in growth of E. coli and S. aureus respectively, relative to untreated samples. Furthermore, the deep groove sample renders 99.9% reduction in growth ofE. colirelative to untreated sample.S. aureusadhesion was governed by TiO2layer & topography, whereasE. coliadhesion was influenced by wettability and the surface roughness of the TiO2layer.S. aureusadhered at valleys between the grooves whereasE. colicells aligned across the ridges. The study reveals the potential of ns-laser for tailoring surface properties to achieve an ideal implant surface.Graphical abstractNanosecond laser texturing imparts anti-adhesive characteristics
Source: expertdata · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Dental materials and restorations · Bone Tissue Engineering Materials · Laser Material Processing Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

References (58)

Show source-provided references
1. C. Joint, R. Registry, Canadian joint replacement registry annual report, 2018. https://secure.cihi.ca/free_products/cjrr-annual-report-2019-en-web.pdf. Accessed 29 Oct 2024).
2. Z. Sun, L. Ma, X. Sun, A.J. Sloan, N.M. O’Brien-Simpson, W. Li, The overview of antimicrobial peptide-coated implants against oral bacterial infections. Aggregate (2023). https://doi.org/10.1002/agt2.30910.1002/agt2.309 ↗
3. N. Sirdeshmukh, G. Dongre, Materials today : proceedings laser micro & nano surface texturing for enhancing osseointegration and antimicrobial effect of biomaterials : a review. Mater. Today Proceed. (2021). https://doi.org/10.1016/j.matpr.2020.12.43310.1016/j.matpr.2020.12.433 ↗
4. Q. Rao, L. Weng, J. Zhang, D. Liu, W. Zhang, S. Chen, J. Chen, X. Li, H. Qiu, Y. Cao, Q. Li, S. Zheng, Research progress in superhydrophobic titanium-based implants for antibacterial applications. Coatings (2023). https://doi.org/10.3390/coatings1302041910.3390/coatings13020419 ↗
5. L. Schweitzer, A. Cunha, T. Pereira, K. Mika, A.M.B. Do Rego, A.M. Ferraria, H. Kieburg, S. Geissler, E. Uhlmann, J. Schoon, Preclinical in vitro assessment of submicron-scale laser surface texturing on ti6al4v. Materials 13, 1–19 (2020). https://doi.org/10.3390/ma1323534210.3390/ma13235342 ↗
6. D. Patil, S. Aravindan, M.K. Wasson, P. Vivekanandan, P.V. Rao, Fast fabrication of superhydrophobic titanium alloy as antibacterial surface using nanosecond laser texturing. J. Micro Nano-Manuf. (2018). https://doi.org/10.1115/1.403809310.1115/1.4038093 ↗
7. G. Wei, M. Tan, S. Attarilar, J. Li, V.V. Uglov, B. Wang, J. Liu, L. Lu, L. Wang, An overview of surface modification, a way toward fabrication of nascent biomedical Ti–6Al–4V alloys. J. Market. Res. 24, 5896–5921 (2023). https://doi.org/10.1016/j.jmrt.2023.04.04610.1016/j.jmrt.2023.04.046 ↗
8. J. Hasan, R.J. Crawford, E.P. Ivanova, Antibacterial surfaces: the quest for a new generation of biomaterials. Trends Biotechnol. 31, 295–304 (2013). https://doi.org/10.1016/j.tibtech.2013.01.01710.1016/j.tibtech.2013.01.017 ↗
9. J. Yu, M. Zhou, L. Zhang, H. Wei, Antibacterial adhesion strategy for dental titanium implant surfaces: from mechanisms to application. J. Funct. Biomater. (2022). https://doi.org/10.3390/jfb1304016910.3390/jfb13040169 ↗
10. R.Y. Siddiquie, A. Gaddam, A. Agrawal, S.S. Dimov, S.S. Joshi, Anti-biofouling properties of femtosecond laser-induced submicron topographies on elastomeric surfaces. Langmuir 36, 5349–5358 (2020). https://doi.org/10.1021/acs.langmuir.0c0075310.1021/acs.langmuir.0c00753 ↗
11. G. Bhagwat, W. O’Connor, I. Grainge, T. Palanisami, Understanding the fundamental basis for biofilm formation on plastic surfaces: role of conditioning films. Front. Microbiol. (2021). https://doi.org/10.3389/fmicb.2021.68711810.3389/fmicb.2021.687118 ↗
12. Y. Zhang, Z. Fan, Y. Xing, S. Jia, Z. Mo, H. Gong, Effect of microtopography on osseointegration of implantable biomaterials and its modification strategies. Front. Bioeng. Biotechnol. (2022). https://doi.org/10.3389/fbioe.2022.98106210.3389/fbioe.2022.981062 ↗
13. C.W. Kang, F.Z. Fang, State of the art of bioimplants manufacturing: part II. Adv. Manuf. 6, 137–154 (2018). https://doi.org/10.1007/s40436-018-0218-910.1007/s40436-018-0218-9 ↗
14. S.C. Vanithakumari, C. Ambar Kumar, C. Thinaharan, G. Ram Kishor, R.P. George, R. Kaul, K.S. Bindra, P. John, Laser patterned titanium surfaces with superior antibiofouling, superhydrophobicity, self-cleaning and durability: role of line spacing. Surf. Coat. Technol. (2021). https://doi.org/10.1016/j.surfcoat.2021.12725710.1016/j.surfcoat.2021.127257 ↗
15. N. Sirdeshmukh, G. Dongre, Results in engineering achieving controlled topography and wettability through laser surface texturing of Ti6Al4V for bioengineering applications. Res. Eng. 17, 100898 (2023). https://doi.org/10.1016/j.rineng.2023.10089810.1016/j.rineng.2023.100898 ↗
16. A. Cunha, A.M. Elie, L. Plawinski, A.P. Serro, Femtosecond laser surface texturing of titanium as a method to reduce the adhesion of Staphylococcus aureus and biofilm formation. Appl. Surf. Sci. 360, 485–493 (2016). https://doi.org/10.1016/j.apsusc.2015.10.10210.1016/j.apsusc.2015.10.102 ↗
17. V. Parmar, A. Kumar, M. Mani Sankar, S. Datta, G. Vijaya Prakash, S. Mohanty, D. Kalyanasundaram, Oxidation facilitated antimicrobial ability of laser micro-textured titanium alloy against gram-positive Staphylococcus aureus for biomedical applications. J. Laser Appl. (2018). https://doi.org/10.2351/1.503986010.2351/1.5039860 ↗
18. I. Singh, S.M. George, A. Tiwari, J. Ramkumar, K. Balani, Influence of laser surface texturing on the wettability and antibacterial properties of metallic, ceramic, and polymeric surfaces. J. Mater. Res. 36, 3985–3999 (2021). https://doi.org/10.1557/s43578-021-00273-810.1557/s43578-021-00273-8 ↗
19. F.P.S. Guastaldi, C.C. De Foggi, L.C.L. Santana, L.G. Vaz, C.E. Vergani, A.C. Guastaldi, Lower susceptibility of laser-irradiated Ti-15Mo surface to methicillin-resistant staphylococcus aureus cells adhesion. Mater. Res. (2019). https://doi.org/10.1590/1980-5373-MR-2019-001210.1590/1980-5373-MR-2019-0012 ↗
20. A.K. Meinshausen, M. Herbster, C. Zwahr, M. Soldera, A. Müller, T. Halle, A.F. Lasagni, J. Bertrand, Aspect ratio of nano/microstructures determines Staphylococcus aureus adhesion on PET and titanium surfaces. J. Appl. Microbiol. 131, 1498–1514 (2021). https://doi.org/10.1111/jam.1503310.1111/jam.15033 ↗
21. Y. Yuan, M.P. Hays, P.R. Hardwidge, J. Kim, Surface characteristics influencing bacterial adhesion to polymeric substrates. RSC Adv. 7, 14254–14261 (2017). https://doi.org/10.1039/c7ra01571b10.1039/c7ra01571b ↗
22. B. Majhy, P. Priyadarshini, A.K. Sen, Effect of surface energy and roughness on cell adhesion and growth-facile surface modification for enhanced cell culture. RSC Adv. 11, 15467–15476 (2021). https://doi.org/10.1039/d1ra02402g10.1039/d1ra02402g ↗
23. Y.R. Chang, E.R. Weeks, W.A. Ducker, Surface topography hinders bacterial surface motility. ACS Appl. Mater. Interfaces 10, 9225–9234 (2018). https://doi.org/10.1021/acsami.7b1671510.1021/acsami.7b16715 ↗
24. M. Krzywicka, J. Szymańska, S. Tofil, A. Malm, A. Grzegorczyk, Surface properties of Ti6Al7Nb alloy: surface free energy and bacteria adhesion. J. Funct. Biomater. (2022). https://doi.org/10.3390/jfb1301002610.3390/jfb13010026 ↗
25. J. Shen, P. Gao, S. Han, R.Y.T. Kao, S. Wu, X. Liu, S. Qian, P.K. Chu, K.M.C. Cheung, K.W.K. Yeung, A tailored positively-charged hydrophobic surface reduces the risk of implant associated infections. Acta Biomater. 114, 421–430 (2020). https://doi.org/10.1016/j.actbio.2020.07.04010.1016/j.actbio.2020.07.040 ↗
26. S. Zheng, M. Bawazir, A. Dhall, H.E. Kim, L. He, J. Heo, G. Hwang, Implication of surface properties, bacterial motility, and hydrodynamic conditions on bacterial surface sensing and their initial adhesion. Front. Bioeng. Biotechnol. (2021). https://doi.org/10.3389/fbioe.2021.64372210.3389/fbioe.2021.643722 ↗
27. S.W. Lee, K.S. Phillips, H. Gu, M. Kazemzadeh-Narbat, D. Ren, How microbes read the map: effects of implant topography on bacterial adhesion and biofilm formation. Biomaterials (2021). https://doi.org/10.1016/j.biomaterials.2020.12059510.1016/j.biomaterials.2020.120595 ↗
28. J. Hasan, K. Chatterjee, Recent advances in engineering topography mediated antibacterial surfaces. Nanoscale 7, 15568–15575 (2015). https://doi.org/10.1039/c5nr04156b10.1039/c5nr04156b ↗
29. X. Luo, S. Yao, H. Zhang, M. Cai, W. Liu, R. Pan, C. Chen, X. Wang, L. Wang, M. Zhong, Biocompatible nano-ripples structured surfaces induced by femtosecond laser to rebel bacterial colonization and biofilm formation. Opt. Laser Technol. (2020). https://doi.org/10.1016/j.optlastec.2019.10597310.1016/j.optlastec.2019.105973 ↗
30. L. Romoli, G. Lazzini, A.H.A. Lutey, F. Fuso, Influence of ns laser texturing of AISI 316L surfaces for reducing bacterial adhesion. CIRP Ann. 69, 529–532 (2020). https://doi.org/10.1016/j.cirp.2020.04.00310.1016/j.cirp.2020.04.003 ↗
31. D.P. Adams, V.C. Hodges, D.A. Hirschfeld, M.A. Rodriguez, J.P. McDonald, P.G. Kotula, Nanosecond pulsed laser irradiation of stainless steel 304L: oxide growth and effects on underlying metal. Surf. Coat. Technol. 222, 1–8 (2013). https://doi.org/10.1016/j.surfcoat.2012.12.04410.1016/j.surfcoat.2012.12.044 ↗
32. N. Sirdeshmukh, G. Dongre, Experimental design for SS 316L-nanosecond laser texturing for bioengineering applications, in Advances in manufacturing engineering. Lecture notes in mechanical engineering. ed. by M.K. Dikshit, A. Soni, J.P. Davim (Springer, Singapore, 2023). https://doi.org/10.1007/978-981-19-4208-2_810.1007/978-981-19-4208-2_8 ↗
33. C.G. Moura, O. Carvalho, L.M.V. Gonçalves, M.F. Cerqueira, R. Nascimento, F. Silva, Laser surface texturing of Ti-6Al-4V by nanosecond laser: surface characterization, Ti-oxide layer analysis and its electrical insulation performance. Mater. Sci. Eng. C 104, 109901 (2019). https://doi.org/10.1016/j.msec.2019.10990110.1016/j.msec.2019.109901 ↗
34. Y. Camurcu, H. Sofu, A.F. Buyuk, S. Gursu, M.A. Kaygusuz, V. Sahin, Two-stage cementless revision total hip arthroplasty for infected primary hip arthroplasties. J. Arthroplasty 30, 1597–1601 (2015). https://doi.org/10.1016/j.arth.2015.03.04010.1016/j.arth.2015.03.040 ↗
35. M.K. Chug, E.J. Brisbois, Recent developments in multifunctional antimicrobial surfaces and applications toward advanced nitric oxide-based biomaterials. ACS Mater. Au (2022). https://doi.org/10.1021/acsmaterialsau.2c0004010.1021/acsmaterialsau.2c00040 ↗
36. A. Braem, L. Van Mellaert, T. Mattheys, D. Hofmans, E. De Waelheyns, L. Geris, J. Anné, J. Schrooten, J. Vleugels, Staphylococcal biofilm growth on smooth and porous titanium coatings for biomedical applications. J. Biomed. Mater. Res. Part A 102, 215–224 (2014). https://doi.org/10.1002/jbm.a.3468810.1002/jbm.a.34688 ↗
37. A. Woźniak, O. Bialas, M. Adamiak, B. Hadzima, J. Szewczenko, The influence of laser texturing on the tribological behavior of titanium alloy Ti6Al4V in medical applications. Arch. Civil Mech. Eng. 24, 1–19 (2024). https://doi.org/10.1007/s43452-024-00960-310.1007/s43452-024-00960-3 ↗
38. A. Samanta, Q. Wang, S.K. Shaw, H. Ding, Roles of chemistry modification for laser textured metal alloys to achieve extreme surface wetting behaviors. Mater. Des. 192, 108744 (2020). https://doi.org/10.1016/j.matdes.2020.10874410.1016/j.matdes.2020.108744 ↗
39. L. Tiainen, P. Abreu, M. Buciumeanu, F. Silva, M. Gasik, R. Serna, O. Carvalho, Novel laser surface texturing for improved primary stability of titanium implants. J. Mech. Behav. Biomed. Mater. 98, 26–39 (2019). https://doi.org/10.1016/j.jmbbm.2019.04.05210.1016/j.jmbbm.2019.04.052 ↗
40. W.L. Yao, J.C.Y. Lin, E. Salamanca, Y.H. Pan, P.Y. Tsai, S.J. Leu, K.C. Yang, H.M. Huang, H.Y. Huang, W.J. Chang, Er, Cr:YSGG laser performance improves biological response on titanium surfaces. Materials (2020). https://doi.org/10.3390/ma1303075610.3390/ma13030756 ↗
41. C. Juan Yang, X. Song Mei, Y. Ling Tian, D. Wei Zhang, Y. Li, X. Ping Liu, Modification of wettability property of titanium by laser texturing. Int. J. Adv. Manuf. Technol. 87, 1663–1670 (2016). https://doi.org/10.1007/s00170-016-8601-910.1007/s00170-016-8601-9 ↗
42. D. Huerta-Murillo, A. García-Girón, J.M. Romano, J.T. Cardoso, F. Cordovilla, M. Walker, S.S. Dimov, J.L. Ocaña, Wettability modification of laser-fabricated hierarchical surface structures in Ti-6Al-4V titanium alloy. Appl. Surf. Sci. 463, 838–846 (2019). https://doi.org/10.1016/j.apsusc.2018.09.01210.1016/j.apsusc.2018.09.012 ↗
43. H. He, R. Hua, X. Li, C. Wang, X. Ning, L. Sun, Fabrication of superhydrophobic Ti-6Al-4V surfaces with single-scale micotextures by using two-step laser irradiation and silanization. Materials (2020). https://doi.org/10.3390/ma1317381610.3390/ma13173816 ↗
44. M. Liu, Z. Yang, L. Dong, Z. Wang, S. Wang, L. Wang, Y. Xie, Q. Zhang, Z. Weng, Y. Tian, Vacuum conditions for tunable wettability transition on laser ablated Ti-6Al-4V alloy surfaces. Colloids Surf A Physicochem Eng Asp 647, 20 (2022). https://doi.org/10.1016/j.colsurfa.2022.12902310.1016/j.colsurfa.2022.129023 ↗
45. S. Ferraris, F. Warchomicka, C. Ramskogler, M. Tortello, A. Cochis, A. Scalia, G. Gautier di Confiengo, J. Keckes, L. Rimondini, S. Spriano, Surface structuring by electron beam for improved soft tissues adhesion and reduced bacterial contamination on Ti-grade 2. J. Mater. Process. Technol. 266, 518–529 (2019). https://doi.org/10.1016/j.jmatprotec.2018.11.02610.1016/j.jmatprotec.2018.11.026 ↗
46. R.G.G.P.D.M. Vadali, Enhancing bactericidal properties of Ti6Al4V surfaces through micro and nano hierarchical laser texturing. Langmuir 40, 16791–16803 (2024). https://doi.org/10.1021/acs.langmuir.4c0117310.1021/acs.langmuir.4c01173 ↗
47. V. Kumaravel, K.M. Nair, S. Mathew, J. Bartlett, J.E. Kennedy, H.G. Manning, B.J. Whelan, N.S. Leyland, S.C. Pillai, Antimicrobial TiO2 nanocomposite coatings for surfaces, dental and orthopaedic implants. Chem. Eng. J. 416, 129071 (2021). https://doi.org/10.1016/j.cej.2021.12907110.1016/j.cej.2021.129071 ↗
48. V.S. Prabhu Suraj, N.S. Ramakrishna, R.S. Babu, P.D. Babu, P. Marimuthu, S. Miryala, C.S. Srinandan, Laser surface texturing inhibits Biofilm formation. Mater. Chem. Phys. (2021). https://doi.org/10.1016/j.matchemphys.2021.12490910.1016/j.matchemphys.2021.124909 ↗
49. S. Shaikh, S. Kedia, D. Singh, M. Subramanian, S. Sinha, Surface texturing of Ti6Al4V alloy using femtosecond laser for superior antibacterial performance. J. Laser Appl. (2019). https://doi.org/10.2351/1.508110610.2351/1.5081106 ↗
50. E. Fadeeva, V.K. Truong, M. Stiesch, B.N. Chichkov, R.J. Crawford, J. Wang, E.P. Ivanova, Bacterial retention on superhydrophobic titanium surfaces fabricated by femtosecond laser ablation. Langmuir 27, 3012–3019 (2011). https://doi.org/10.1021/la104607g10.1021/la104607g ↗
51. N. Chik, W.S. Wan Md Zain, A.J. Mohamad, M.Z. Sidek, W.H. Wan Ibrahim, A. Reif, J.H. Rakebrandt, W. Pfleging, X. Liu, Bacterial Adhesion on the Titanium and Stainless-Steel Surfaces Undergone Two Different Treatment Methods: Polishing and Ultrafast Laser Treatment, In: IOP Conference Series: Materials Science and Engineering, Institute of Physics Publishing, 2018. https://doi.org/10.1088/1757-899X/358/1/012034.10.1088/1757-899X/358/1/012034 ↗
52. A.C. Ionescu, E. Brambilla, F. Azzola, M. Ottobelli, G. Pellegrini, L.A. Francetti, Laser microtextured titanium implant surfaces reduce in vitro and in situ oral biofilm formation. PLoS ONE 13, 1–19 (2018). https://doi.org/10.1371/journal.pone.020226210.1371/journal.pone.0202262 ↗
53. S. Kheur, N. Singh, D. Bodas, J.Y. Rauch, S. Jambhekar, M. Kheur, J. Rajwade, Nanoscale silver depositions inhibit microbial colonization and improve biocompatibility of titanium abutments. Colloids Surf. B 159, 151–158 (2017). https://doi.org/10.1016/j.colsurfb.2017.07.07910.1016/j.colsurfb.2017.07.079 ↗
54. V. Madiwal, J. Rajwade, Silver-deposited titanium as a prophylactic ‘nano coat’ for peri-implantitis. Nanoscale Adv. 6, 2113–2128 (2024). https://doi.org/10.1039/d3na00898c10.1039/d3na00898c ↗
55. V. Madiwal, B. Khairnar, J. Rajwade, Enhanced antibacterial activity and superior biocompatibility of cobalt-deposited titanium discs for possible use in implant dentistry. IScience (2024). https://doi.org/10.1016/j.isci.2024.10882710.1016/j.isci.2024.108827 ↗
56. X. Wang, Z. Jia, J. Ma, D. Han, X. Qi, C. Gui, W. Liu, Prediction method of radial heat-affected zone width in nanosecond pulsed laser ablation of TC4 titanium alloy. Int. J. Adv. Manuf. Technol. 121, 2663–2670 (2022). https://doi.org/10.1007/s00170-022-09408-510.1007/s00170-022-09408-5 ↗
57. A.A. Miles, S.S. Misra, J.O. Irwin, The estimation of the bactericidal power of the blood. J. Hyg. 38(6), 732–749 (1938). http://www.jstor.org/stable/3859638
58. Y. Sasikumar, M.M. Solomon, L.O. Olasunkanmi, E.E. Ebenso, Effect of surface treatment on the bioactivity and electrochemical behavior of magnesium alloys in simulated body fluid. Mater. Corros. 68, 776–790 (2017). https://doi.org/10.1002/maco.20160931710.1002/maco.201609317 ↗