Characterization of a new triaxial nanofiber membrane and osteogenic differentiation capacity of human bone marrow mesenchymal stem cells for bone repair
Source abstract
Abstract
A triaxial nanofiber membrane was developed using a custom triaxial nozzle during electrospinning. The membrane consisted of three layers (outer middle (PVA-Collagen Type I-BMP-2), and inner (PVA-nHAP)). TEM revealed the diameters of the outer, middle, and inner layers as 167.5, 142.6, and 119.3 nm, respectively. The triaxial nanofiber membrane exhibited superior tensile strength (36 MPa) and elastic modulus (2.5 GPa), along with a roughness of 359 nm and hydrophilic properties. In addition, it demonstrated a 200% swelling ratio and reduced bacterial colonization by 10.8-fold. Both Calcium deposition and ALP activity were significantly higher and increased expression of BSP (eightfold), OCN (13-fold), and OPN (488-fold) genes were observed in membrane + OST groups (p < 0.05), compared to MSC and OST groups. These findings highlight that the membrane has potential for enhance bone repair by combining well-structured, antimicrobial, mechanical, and osteoinductive properties.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Electrospun Nanofibers in Biomedical Applications · Mesenchymal stem cell research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (91)
Show source-provided references
1. X. Niu, L. Wang, M. Xu, M. Qin, L. Zhao, Y. Wei, Y. Hu, X. Lian, Z. Liang, S. Chen, W. Chen, D. Huang, Electrospun polyamide-6/chitosan nanofibers reinforced nano-hydroxyapatite/polyamide-6 composite bilayered membranes for guided bone regeneration. Carbohydr. Polym. 260, 117769 (2021). https://doi.org/10.1016/j.carbpol.2021.11776910.1016/j.carbpol.2021.117769 ↗
2. P. Bhattacharjee, P. Gupta, M.J. Christakiran, S.K. Nandi and B.B. Mandal, in nanostructures for the engineering of cells: tissues and organs, ed. A. M. Grumezescu , Silk-based matrices for bone tissue engineering applications, in Nanostructures for the Engineering of Cells, Tissues and Organs, (William Andrew, 2018). https://doi.org/10.1016/B978-0-12-813665-2.00012-010.1016/B978-0-12-813665-2.00012-0 ↗
3. K. Ghosal, R. Augustine, A. Zaszczynska, M. Barman, A. Jain, A. Hasan, N. Kalarikkal, P. Sajkiewicz, S. Thomas, Novel drug delivery systems based on triaxial electrospinning based nanofibers. React. Funct. Polym. 163, 104895 (2021). https://doi.org/10.1016/j.reactfunctpolym.2021.10489510.1016/j.reactfunctpolym.2021.104895 ↗
4. D. Li, L. Tao, Y. Shen, B. Sun, X. Xie, Q. Ke, X. Mo, B. Deng, Fabrication of multilayered nanofiber scaffolds with a highly aligned nanofiber yarn for anisotropic tissue regeneration. ACS Omega 5(38), 24340 (2020). https://doi.org/10.1021/acsomega.0c0255410.1021/acsomega.0c02554 ↗
5. Y. Su, Q. Su, W. Liu, M. Lim, J.R. Venugopal, X. Mo, S. Ramakrishna, S.S. Al-Deyab, M. El-Newehy, Controlled release of bone morphogenetic protein 2 and dexamethasone loaded in core–shell PLLACL–collagen fibers for use in bone tissue engineering. Acta Biomater. 8(2), 763 (2012). https://doi.org/10.1016/j.actbio.2011.11.00210.1016/j.actbio.2011.11.002 ↗
6. M. Tang, K. Xu, H. Shang, X. Li, X. He, L. Ke, M. Xie, Z. Zhou, C. Liu, S. Du, Y. Wang, J. Gao, H. Xu, Biomineralization of bone-like hydroxyapatite to upgrade the mechanical and osteoblastic performances of poly(lactic acid) scaffolds. Int. J. Biol. Macromol. 226, 1273 (2023). https://doi.org/10.1016/j.ijbiomac.2022.11.24010.1016/j.ijbiomac.2022.11.240 ↗
7. X. Wang, W. Zheng, Z. Bai, S. Huang, K. Jiang, H. Liu, L. Liu, Mimicking bone matrix through coaxial electrospinning of core-shell nanofibrous scaffold for improving neurogenesis bone regeneration. Biomater. Adv. 145, 213246 (2023). https://doi.org/10.1016/j.bioadv.2022.21324610.1016/j.bioadv.2022.213246 ↗
8. R. Eivazzadeh-Keihan, K.K. Chenab, R. Taheri-Ledari, J. Mosafer, S.M. Hashemi, A. Mokhtarzadeh, A. Maleki, M.R. Hamblin, Recent advances in the application of mesoporous silica-based nanomaterials for bone tissue engineering. Mater. Sci. Eng. C 107, 110267 (2020). https://doi.org/10.1016/j.msec.2019.11026710.1016/j.msec.2019.110267 ↗
9. K. Ma, C. Liao, L. Huang, R. Liang, J. Zhao, L. Zheng, W. Su, Electrospun PCL/MoS2 nanofiber membranes combined with NIR-triggered photothermal therapy to accelerate bone regeneration. Small 17(51), 2104747 (2021). https://doi.org/10.1002/smll.20210474710.1002/smll.202104747 ↗
10. Z. Gu, S. Fan, S.C. Kundu, X. Yao, Y. Zhang, Fiber diameters and parallel patterns: proliferation and osteogenesis of stem cells. Regenerat. Biomater. (2023). https://doi.org/10.1093/rb/rbad00110.1093/rb/rbad001 ↗
11. L. Zhang, Y. Dong, Y. Liu, X. Liu, Z. Wang, J. Wan, X. Yu, S. Wang, Multifunctional hydrogel/platelet-rich fibrin/nanofibers scaffolds with cell barrier and osteogenesis for guided tissue regeneration/guided bone regeneration applications. Int. J. Biol. Macromol. 253, 126960 (2023). https://doi.org/10.1016/j.ijbiomac.2023.12696010.1016/j.ijbiomac.2023.126960 ↗
12. D.J. Lim, Bone mineralization in electrospun-based bone tissue engineering. Polymers (Basel). 14(10), 2123 (2022). https://doi.org/10.3390/polym1410212310.3390/polym14102123 ↗
13. A. Mickova, M. Buzgo, O. Benada, M. Rampichova, Z. Fisar, E. Filova, M. Tesarova, D. Lukas, E. Amler, Core/shell nanofibers with embedded liposomes as a drug delivery system. Biomacromol 13(4), 952 (2012). https://doi.org/10.1021/bm201811810.1021/bm2018118 ↗
14. C. Huang, J. Dong, Y. Zhang, S. Chai, X. Wang, S. Kang, D. Yu, P. Wang, Q. Jiang, Gold nanoparticles-loaded polyvinylpyrrolidone/ethylcellulose coaxial electrospun nanofibers with enhanced osteogenic capability for bone tissue regeneration. Mater. Design. 212, 110240 (2021). https://doi.org/10.1016/j.matdes.2021.11024010.1016/j.matdes.2021.110240 ↗
15. S.B. Abdollahi Boraei, J. Nourmohammadi, B. Bakhshandeh, M.M. Dehghan, H. Gholami, Z. Gonzalez, A.J. Sanchez-Herencia, B. Ferrari, Capability of core-sheath polyvinyl alcohol–polycaprolactone emulsion electrospun nanofibrous scaffolds in releasing strontium ranelate for bone regeneration. Biomed. Mater. 16(2), 025009 (2021). https://doi.org/10.1088/1748-605X/abdb0710.1088/1748-605X/abdb07 ↗
16. Y.-Z. Long, X. Yan, X.-X. Wang, J. Zhang and M. Yu, in Electrospinning: nanofabrication and applications, ed. by B. Ding, X. Wang and J. Yu, Electrospinning: the setup and procedure, (William Andrew Publishing, 2019), pp. 21. https://doi.org/10.1016/B978-0-323-51270-1.00002-910.1016/B978-0-323-51270-1.00002-9 ↗
17. Ö. Altundag, M. Öteyaka, B. Çelebi-Saltik, Co- and triaxial electrospinning for stem cell-based bone regeneration. Curr. Stem Cell Res. Ther. (2023). https://doi.org/10.2174/1574888x1866623081809421610.2174/1574888x18666230818094216 ↗
18. D. Han, A.J. Steckl, Triaxial electrospun nanofiber membranes for controlled dual release of functional molecules. ACS Appl. Mater. Interfaces 5(16), 8241 (2013). https://doi.org/10.1021/am402376c10.1021/am402376c ↗
19. W. Liu, C. Ni, D.B. Chase, J.F. Rabolt, Preparation of multilayer biodegradable nanofibers by triaxial electrospinning. ACS Macro Lett. 2(6), 466 (2013). https://doi.org/10.1021/mz400068810.1021/mz4000688 ↗
20. R. Taheri-Ledari, W. Zhang, M. Radmanesh, N. Cathcart, A. Maleki, V. Kitaev, Plasmonic photothermal release of docetaxel by gold nanoparticles incorporated onto halloysite nanotubes with conjugated 2D8-E3 antibodies for selective cancer therapy. J. Nanobiotechnol. 19(1), 239 (2021). https://doi.org/10.1186/s12951-021-00982-610.1186/s12951-021-00982-6 ↗
21. S. Panzavolta, C. Gualandi, A. Fiorani, B. Bracci, M.L. Focarete, A. Bigi, Fast Coprecipitation of calcium phosphate nanoparticles inside gelatin nanofibers by tricoaxial electrospinning. J. Nanomater. 2016, 4235235 (2016). https://doi.org/10.1155/2016/423523510.1155/2016/4235235 ↗
22. D. Han, S. Sherman, S. Filocamo, A.J. Steckl, Long-term antimicrobial effect of nisin released from electrospun triaxial fiber membranes. Acta Biomater. 53, 242 (2017). https://doi.org/10.1016/j.actbio.2017.02.02910.1016/j.actbio.2017.02.029 ↗
23. A.A. Aldana, G.A. Abraham, Current advances in electrospun gelatin-based scaffolds for tissue engineering applications. Int. J. Pharm. 523(2), 441 (2017). https://doi.org/10.1016/j.ijpharm.2016.09.04410.1016/j.ijpharm.2016.09.044 ↗
24. T. Eren Boncu, N. Ozdemir, Electrospinning of ampicillin trihydrate loaded electrospun PLA nanofibers i: effect of polymer concentration and PCL addition on its morphology, drug delivery and mechanical properties. Int. J. Polym. Mater. Polym. Biomater. 71(9), 669 (2022). https://doi.org/10.1080/00914037.2021.187605710.1080/00914037.2021.1876057 ↗
25. D. Cho, A.N. Netravali, Y.L. Joo, Mechanical properties and biodegradability of electrospun soy protein Isolate/PVA hybrid nanofibers. Polym. Degrad. Stab. 97(5), 747 (2012). https://doi.org/10.1016/j.polymdegradstab.2012.02.00710.1016/j.polymdegradstab.2012.02.007 ↗
26. S.B. Qasim, M.S. Zafar, S. Najeeb, Z. Khurshid, A.H. Shah, S. Husain, I.U. Rehman, Electrospinning of chitosan-based solutions for tissue engineering and regenerative medicine. Int. J. Mol. Sci. 19(2), 404 (2018). https://doi.org/10.3390/ijms1902040710.3390/ijms19020407 ↗
27. Z. Mbese, S. Alven, B.A. Aderibigbe, Collagen-based nanofibers for skin regeneration and wound dressing applications. Polymers 13(24), 4368 (2021). https://doi.org/10.3390/polym1324436810.3390/polym13244368 ↗
28. H.W. Ju, O.J. Lee, J.M. Lee, B.M. Moon, H.J. Park, Y.R. Park, M.C. Lee, S.H. Kim, J.R. Chao, C.S. Ki, C.H. Park, Wound healing effect of electrospun silk fibroin nanomatrix in burn-model. Int. J. Biol. Macromol. 85, 29 (2016). https://doi.org/10.1016/j.ijbiomac.2015.12.05510.1016/j.ijbiomac.2015.12.055 ↗
29. M. Najafiasl, S. Osfouri, R. Azin, S. Zaeri, Alginate-based electrospun core/shell nanofibers containing dexpanthenol: a good candidate for wound dressing. J. Drug Deliv. 57, 101708 (2020). https://doi.org/10.1016/j.jddst.2020.10170810.1016/j.jddst.2020.101708 ↗
30. S.A. Salim, S.A. Loutfy, E.M. El-Fakharany, T.H. Taha, Y. Hussien, E.A. Kamoun, Influence of chitosan and hydroxyapatite incorporation on properties of electrospun PVA/HA nanofibrous mats for bone tissue regeneration: Nanofibers optimization and in-vitro assessment. J. Drug Deliv. 62, 102417 (2021). https://doi.org/10.1016/j.jddst.2021.10241710.1016/j.jddst.2021.102417 ↗
31. Y. Kan, A.I. Salimon, A.M. Korsunsky, On the electrospinning of nanostructured collagen-PVA fiber mats. Mater Today Proc. 33, 2013 (2020). https://doi.org/10.1016/j.matpr.2020.07.62110.1016/j.matpr.2020.07.621 ↗
32. J.M. Anaya Mancipe, M. Lopes Dias, R.M.D.S. Moreira Thiré, Type I collagen – poly(vinyl alcohol) electrospun nanofibers: FTIR study of the collagen helical structure preservation. Polym. PlastTechnol. Mater. 61(8), 846 (2022). https://doi.org/10.1080/25740881.2022.202988710.1080/25740881.2022.2029887 ↗
33. S. Guo, L. He, R. Yang, B. Chen, X. Xie, B. Jiang, T. Weidong, Y. Ding, Enhanced effects of electrospun collagen-chitosan nanofiber membranes on guided bone regeneration. J. Biomater. Sci. Polym E. 31(2), 155 (2020). https://doi.org/10.1080/09205063.2019.168092710.1080/09205063.2019.1680927 ↗
34. D.A. Canales, N. Piñones, M. Saavedra, C. Loyo, H. Palza, L. Peponi, A. Leonés, R.V. Baier, A.R. Boccaccini, A. Grünelwald, P.A. Zapata, Fabrication and assessment of bifunctional electrospun poly(l-lactic acid) scaffolds with bioglass and zinc oxide nanoparticles for bone tissue engineering. Int. J. Biol. Macromol. 228, 78 (2023). https://doi.org/10.1016/j.ijbiomac.2022.12.19510.1016/j.ijbiomac.2022.12.195 ↗
35. D. dos Santos Gomes, R. de Sousa Victor, B.V. de Sousa, G. de Araújo Neves, L.N. de Lima Santana, R.R. Menezes, Ceramic nanofiber materials for wound healing and bone regeneration: a brief review. Materials. 15(11), 3909 (2022)10.3390/ma15113909 ↗
36. T. Aminatun, Y.W. Suciati, M. Sari, K.A. Sari, W.P. Alamsyah, Y. Yusuf, Biopolymer-based polycaprolactone-hydroxyapatite scaffolds for bone tissue engineering. Int. J. Polym. Mater. Polym. Biomater. 72(5), 376 (2023). https://doi.org/10.1080/00914037.2021.201831510.1080/00914037.2021.2018315 ↗
37. G. Di Pompo, A. Liguori, M. Carlini, S. Avnet, M. Boi, N. Baldini, M.L. Focarete, M. Bianchi, C. Gualandi, G. Graziani, Electrospun fibers coated with nanostructured biomimetic hydroxyapatite: a new platform for regeneration at the bone interfaces. Biomater. Adv. 144, 213231 (2023). https://doi.org/10.1016/j.bioadv.2022.21323110.1016/j.bioadv.2022.213231 ↗
38. Y. Hartatiek, M.I. Wuriantika, J. Utomo, M. Nurhuda, Masruroh and D.J.D.H. Santjojo, Nanostructure, porosity and tensile strength of PVA/Hydroxyapatite composite nanofiber for bone tissue engineering. Mater. Today Proc. 44, 3203 (2021). https://doi.org/10.1016/j.matpr.2020.11.43810.1016/j.matpr.2020.11.438 ↗
39. M. Laurenti, V. Cauda, ZnO nanostructures for tissue engineering applications. Nanomater. 7(11), 374 (2017). https://doi.org/10.3390/nano711037410.3390/nano7110374 ↗
40. H. Nekounam, S. Samani, H. Samadian, M.A. Shokrgozar, R. Faridi-Majidi, Zinc oxide-carbon nanofiber (ZnO-CNF) nanocomposite for bone tissue engineering: an inquiry into structural, physical and biological properties. Mater. Chem. Phys. 295, 127052 (2023). https://doi.org/10.1016/j.matchemphys.2022.12705210.1016/j.matchemphys.2022.127052 ↗
41. P. Moradipour, M. Limoee, S. Janfaza, L. Behbood, Core-Shell Nanofibers based on polycaprolactone/polyvinyl alcohol and polycaprolactone/collagen for biomedical applications. J. Pharm. Innov. 17(3), 911 (2022). https://doi.org/10.1007/s12247-021-09568-z10.1007/s12247-021-09568-z ↗
42. M. Khan, D. Kharaghani, N. Nishat, A. Shahzad, T. Hussain, Z. Khatri, C. Zhu, I.-S. Kim, Preparation and characterizations of multifunctional PVA/ZnO nanofibers composite membranes for surgical gown application. J. Mater. Res. Technol. (2018). https://doi.org/10.1016/j.jmrt.2018.08.01310.1016/j.jmrt.2018.08.013 ↗
43. H. Gheisari, E. Karamian, M. Abdellahi, A novel hydroxyapatite –hardystonite nanocomposite ceramic. Ceram. Internat. 41, 5967 (2015). https://doi.org/10.1016/j.ceramint.2015.01.03310.1016/j.ceramint.2015.01.033 ↗
44. G.-M. Kim, in Nanofibers, ed. A. Kumar, Fabrication of bio-nanocomposite nanofibers mimicking the mineralized hard tissues via electrospinning process, (IntechOpen, Rijeka, 2010). https://doi.org/10.5772/814810.5772/8148 ↗
45. S. Zahid, H. Khalid, F. Ikram, H. Iqbal, M. Samie, L. Shahzadi, A.T. Shah, M. Yar, A.A. Chaudhry, S.J. Awan, A.F. Khan, I.u. Rehman, Bi-layered α-tocopherol acetate loaded membranes for potential wound healing and skin regeneration. Mater. Sci. Eng. (2019). https://doi.org/10.1016/j.msec.2019.03.08010.1016/j.msec.2019.03.080 ↗
46. F. Azhar, A. Olad, R. Salehi, Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds. Design. Monom. Polym. 17, 654 (2014). https://doi.org/10.1080/15685551.2014.90762110.1080/15685551.2014.907621 ↗
47. F. Loi, L.A. Córdova, J. Pajarinen, T.H. Lin, Z. Yao, S.B. Goodman, Inflammation, fracture and bone repair. Bone 86, 119 (2016). https://doi.org/10.1016/j.bone.2016.02.02010.1016/j.bone.2016.02.020 ↗
48. H. Çetinkaya, G. Özden-Gürcan, M.Ö. Öteyaka, M. Şahin, G.K. Mağden, Effect of electrospinning parameters on the production of polyvinyl alcohol (PVA)/collagen (Type I) nanofiber membranes and the use of an adaptive neuro-fuzzy inference system for evaluating nanofiber diameters. J. Polym. Res. 31(12), 358 (2024). https://doi.org/10.1007/s10965-024-04203-010.1007/s10965-024-04203-0 ↗
49. S. Nepal, J. Si, S. Ishikawa, M. Nishikawa, Y. Sakai, A.M. Akimoto, H. Okada, S. Ohba, U.I. Chung, T. Sakai, H. Hojo, Injectable phase-separated tetra-armed poly(ethylene glycol) hydrogel scaffold allows sustained release of growth factors to enhance the repair of critical bone defects. Regen Ther. 25, 24 (2024). https://doi.org/10.1016/j.reth.2023.11.00810.1016/j.reth.2023.11.008 ↗
50. B. Çelebi-Saltik, S. Babadag, E. Ballikaya, S. Pat, M. Öteyaka, Osteogenic differentiation capacity of dental pulp stem cells on 3D printed polyurethane/boric acid scaffold. Biol. Trace Elem. Res. 202(4), 1446 (2024). https://doi.org/10.1007/s12011-023-03781-210.1007/s12011-023-03781-2 ↗
51. D.P. Dowling, I.S. Miller, M. Ardhaoui, W.M. Gallagher, Effect of surface wettability and topography on the adhesion of osteosarcoma cells on plasma-modified polystyrene. J. Biomater. Appl. 26(3), 327 (2011). https://doi.org/10.1177/088532821037214810.1177/0885328210372148 ↗
52. B. Majhy, P. Priyadarshini, A. Sen, Effect of surface energy and roughness on cell adhesion and growth -facile surface modification for enhanced cell culture †. RSC Adv. 11, 15467 (2021). https://doi.org/10.1039/d1ra02402g10.1039/d1ra02402g ↗
53. J.J. Ahire, D.D. Robertson, A.J. van Reenen, L.M.T. Dicks, Surfactin-loaded polyvinyl alcohol (PVA) nanofibers alters adhesion of Listeria monocytogenes to polystyrene. Mater. Sci. Eng. C Mater. Biol. Appl. 77, 27 (2017). https://doi.org/10.1016/j.msec.2017.03.24810.1016/j.msec.2017.03.248 ↗
54. I. Jipa, A. Stoica, M. Stroescu, L.M. Dobre, T. Dobre, S. Jinga, C. Tardei, Potassium sorbate release from poly(vinyl alcohol)–bacterial cellulose films. Chem. Papers. 66, 138 (2012). https://doi.org/10.2478/s11696-011-0068-410.2478/s11696-011-0068-4 ↗
55. Ö. Aktürk, D. Keskin, Collagen/PEO/gold nanofibrous matrices for skin tissue engineering. Turk. J. Biol. 40(2), 380–398 (2016). https://doi.org/10.3906/biy-1502-4910.3906/biy-1502-49 ↗
56. P. Pal, P. Dadhich, P. Srivas, B. Das, D. Moulik and S. Dhara, 2017 Bilayered nanofibrous 3D hierarchy as skin rudiment by emulsion electrospinning for burn wound management. Biomater. Sci. https://doi.org/10.1039/C7BM00174F10.1039/C7BM00174F ↗
57. A. Chandrasekaran, S. Suresh, A. Dakshanamoorthy, Synthesis and characterization of nano-hydroxyapatite (n-HAP) using the wet chemical technique. Int. J. phys. Sci. 8, 1639 (2013). https://doi.org/10.5897/IJPS2013.399010.5897/IJPS2013.3990 ↗
58. K. Handore, S. Bhavsar, A. Horne, P. Chhattise, K. Mohite, J. Ambekar, N. Pande, V. Chabukswar, Novel green route of synthesis of ZnO nanoparticles by using natural biodegradable polymer and its application as a catalyst for oxidation of aldehydes. J. Macromol. Sci. Part A Pure Appl. Chem. 51, 941 (2014). https://doi.org/10.1080/10601325.2014.96707810.1080/10601325.2014.967078 ↗
59. N. Jayarambabu, Germination and growth characteristics of mungbean seeds (Vigna radiata L.) affected by synthesized zinc oxide nanoparticles. Int. J. Current Eng. Technol. 4, 5 (2014)
60. A.S. Asran, S. Henning, G.H. Michler, Polyvinyl alcohol–collagen–hydroxyapatite biocomposite nanofibrous scaffold: mimicking the key features of natural bone at the nanoscale level. Polymer 51(4), 868 (2010). https://doi.org/10.1016/j.polymer.2009.12.04610.1016/j.polymer.2009.12.046 ↗
61. A.L. Kwansa, R. De Vita, J.W. Freeman, Tensile mechanical properties of collagen type I and its enzymatic crosslinks. Biophys. Chem. 214–215, 1 (2016). https://doi.org/10.1016/j.bpc.2016.04.00110.1016/j.bpc.2016.04.001 ↗
62. J.-C. Park, T. Ito, K.-O. Kim, K.-W. Kim, B.-S. Kim, M.-S. Khil, H.-Y. Kim, I.-S. Kim, Electrospun poly(vinyl alcohol) nanofibers: effects of degree of hydrolysis and enhanced water stability. Polym. J. 42(3), 273 (2010). https://doi.org/10.1038/pj.2009.34010.1038/pj.2009.340 ↗
63. M. Norouzi, M. Montazer, T. Harifi, P. Karimi, Flower buds like PVA/ZnO composite nanofibers assembly: antibacterial, in vivo wound healing, cytotoxicity and histological studies. Polym. Test. 93, 106914 (2021). https://doi.org/10.1016/j.polymertesting.2020.10691410.1016/j.polymertesting.2020.106914 ↗
64. S. Jeong, S.-G. Oh, Antiacne effects of PVA/ZnO composite nanofibers crosslinked by citric acid for facial sheet masks. Int. J. Polym. Sci. 2022, 4694921 (2022). https://doi.org/10.1155/2022/469492110.1155/2022/4694921 ↗
65. A. Pérez-Nava, A.E. Espino-Saldaña, E. Pereida-Jaramillo, J. Hernández-Vargas, A. Martínez-Torres, M.O. Vázquez-Lepe, J.D. Mota-Morales, B.A. Frontana Uribe, J. Betzabe González-Campos, Surface collagen functionalization of electrospun poly(vinyl alcohol) scaffold for tissue engineering. Process Biochem. 126, 1 (2023). https://doi.org/10.1016/j.procbio.2022.12.02210.1016/j.procbio.2022.12.022 ↗
66. N. Angel Villegas, M.J. Silvero Compagnucci, M. Sainz Ajá, D.M. Rocca, M.C. Becerra, G. Fabián Molina, S.D. Palma, Novel antibacterial resin-based filling material containing nanoparticles for the potential one-step treatment of caries. J. Healthcare Eng. 2019, 6367919 (2019). https://doi.org/10.1155/2019/636791910.1155/2019/6367919 ↗
67. S.T. Khan, J. Ahmad, M. Ahamed, J. Musarrat, A.A. Al-Khedhairy, Zinc oxide and titanium dioxide nanoparticles induce oxidative stress, inhibit growth, and attenuate biofilm formation activity of Streptococcus mitis. J. Biol. Inorg. Chem. 21(3), 295 (2016). https://doi.org/10.1007/s00775-016-1339-x10.1007/s00775-016-1339-x ↗
68. K.R. Raghupathi, R.T. Koodali, A.C. Manna, Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir 27(7), 4020 (2011). https://doi.org/10.1021/la104825u10.1021/la104825u ↗
69. J.C. Pound, D.W. Green, H.I. Roach, S. Mann, R.O. Oreffo, An ex vivo model for chondrogenesis and osteogenesis. Biomaterials 28(18), 2839 (2007). https://doi.org/10.1016/j.biomaterials.2007.02.02910.1016/j.biomaterials.2007.02.029 ↗
70. Y. Deng, X. Liu, A. Xu, L. Wang, Z. Luo, Y. Zheng, F. Deng, J. Wei, Z. Tang, S. Wei, Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone-nanohydroxyapatite composite. Int. J. Nanomed. 10, 1425 (2015). https://doi.org/10.2147/IJN.S7555710.2147/IJN.S75557 ↗
71. M. Ermis, E. Antmen, V. Hasirci, Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: a review from the tissue engineering perspective. Bioact Mater. 3(3), 355 (2018). https://doi.org/10.1016/j.bioactmat.2018.05.00510.1016/j.bioactmat.2018.05.005 ↗
72. S. Teixeira, F.J. Monteiro, M.P. Ferraz, R. Vilar, S. Eugenio, Laser surface treatment of hydroxyapatite for enhanced tissue integration: surface characterization and osteoblastic interaction studies. J. Biomed. Mater. Res. A 81(4), 920 (2007). https://doi.org/10.1002/jbm.a.3107310.1002/jbm.a.31073 ↗
73. V. Lukasova, M. Buzgo, V. Sovkova, J. Dankova, M. Rampichova, E. Amler, Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides. Cell Prolif. (2017). https://doi.org/10.1111/cpr.1235710.1111/cpr.12357 ↗
74. W. Wang, K.W.K. Yeung, Bone grafts and biomaterials substitutes for bone defect repair: a review. Bioact. Mater. 2(4), 224 (2017). https://doi.org/10.1016/j.bioactmat.2017.05.00710.1016/j.bioactmat.2017.05.007 ↗
75. A. Cheng, Z. Schwartz, A. Kahn, X. Li, Z. Shao, M. Sun, Y. Ao, B.D. Boyan, H. Chen, Advances in porous scaffold design for bone and cartilage tissue engineering and regeneration. Tissue Eng. Part B Rev. 25(1), 14 (2019). https://doi.org/10.1089/ten.TEB.2018.011910.1089/ten.TEB.2018.0119 ↗
76. S.N. Alhosseini, F. Moztarzadeh, M. Mozafari, S. Asgari, M. Dodel, A. Samadikuchaksaraei, S. Kargozar, N. Jalali, Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering. Int. J. Nanomed. 7, 25 (2012). https://doi.org/10.2147/IJN.S2537610.2147/IJN.S25376 ↗
77. S. Vimalraj, Alkaline phosphatase: structure, expression and its function in bone mineralization. Gene 754, 144855 (2020). https://doi.org/10.1016/j.gene.2020.14485510.1016/j.gene.2020.144855 ↗
78. S. Rasi Ghaemi, B. Delalat, X. Ceto, F.J. Harding, J. Tuke, N.H. Voelcker, Synergistic influence of collagen I and BMP 2 drives osteogenic differentiation of mesenchymal stem cells: a cell microarray analysis. Acta Biomater. 34, 41 (2016). https://doi.org/10.1016/j.actbio.2015.07.02710.1016/j.actbio.2015.07.027 ↗
79. C. Li, C. Vepari, H.J. Jin, H.J. Kim, D.L. Kaplan, Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 27(16), 3115 (2006). https://doi.org/10.1016/j.biomaterials.2006.01.02210.1016/j.biomaterials.2006.01.022 ↗
80. M. Ishijima, K. Tsuji, S.R. Rittling, T. Yamashita, H. Kurosawa, D.T. Denhardt, A. Nifuji, Y. Ezura, M. Noda, Osteopontin is required for mechanical stress-dependent signals to bone marrow cells. J. Endocrinol. 193(2), 235 (2007). https://doi.org/10.1677/joe.1.0670410.1677/joe.1.06704 ↗
81. H. Li, Y. Liu, Q. Zhang, Y. Jing, S. Chen, Z. Song, J. Yan, Y. Li, X. Wu, X. Zhang, Y. Zhang, J. Case, M. Yu, D.A. Ingram, F.C. Yang, Ras dependent paracrine secretion of osteopontin by Nf1+/- osteoblasts promote osteoclast activation in a neurofibromatosis type I murine model. Pediatr. Res. 65(6), 613 (2009). https://doi.org/10.1203/PDR.0b013e3181a1c60710.1203/PDR.0b013e3181a1c607 ↗
82. M.F. Pittenger, A.M. Mackay, S.C. Beck, R.K. Jaiswal, R. Douglas, J.D. Mosca, M.A. Moorman, D.W. Simonetti, S. Craig, D.R. Marshak, Multilineage potential of adult human mesenchymal stem cells. Science 284(5411), 143 (1999). https://doi.org/10.1126/science.284.5411.14310.1126/science.284.5411.143 ↗
83. W. Huang, S. Yang, J. Shao, Y.P. Li, Signaling and transcriptional regulation in osteoblast commitment and differentiation. Front Biosci 12, 3068 (2007). https://doi.org/10.2741/229610.2741/2296 ↗
84. S. Zhu, W. Chen, A. Masson, Y.P. Li, Cell signaling and transcriptional regulation of osteoblast lineage commitment, differentiation, bone formation, and homeostasis. Cell Discov. 10(1), 71 (2024). https://doi.org/10.1038/s41421-024-00689-610.1038/s41421-024-00689-6 ↗
85. E.J. Chung, K.B. Chien, B.A. Aguado, R.N. Shah, Osteogenic potential of BMP-2-releasing self-assembled membranes. Tissue Eng. Part A 19(23–24), 2664 (2013). https://doi.org/10.1089/ten.TEA.2012.066710.1089/ten.TEA.2012.0667 ↗
86. E.C. Dede, M. Gizer, F. Korkusuz, Z. Bal, H. Ishiguro, H. Yoshikawa, T. Kaito, P. Korkusuz, A pilot study: nano-hydroxyapatite-PEG/PLA containing low dose rhBMP2 stimulates proliferation and osteogenic differentiation of human bone marrow derived mesenchymal stem cells. JOR Spine. 6(3), 1258 (2023). https://doi.org/10.1002/jsp2.125810.1002/jsp2.1258 ↗
87. C.M. França, G. Thrivikraman, A. Athirasala, A. Tahayeri, L.B. Gower, L.E. Bertassoni, The influence of osteopontin-guided collagen intrafibrillar mineralization on pericyte differentiation and vascularization of engineered bone scaffolds. J. Biomed. Mater. Res. 107(5), 1522 (2019). https://doi.org/10.1002/jbm.b.3424410.1002/jbm.b.34244 ↗
88. V. Chopra, J. Thomas, A. Sharma, V. Panwar, S. Kaushik, S. Sharma, K. Porwal, C. Kulkarni, S. Rajput, H. Singh, K. Jagavelu, N. Chattopadhyay, D. Ghosh, Synthesis and evaluation of a zinc eluting rGO/hydroxyapatite nanocomposite optimized for bone augmentation. ACS Biomater. Sci. Eng. 6(12), 6710 (2020). https://doi.org/10.1021/acsbiomaterials.0c0037010.1021/acsbiomaterials.0c00370 ↗
89. Z. Zhong, X. Wu, Y. Wang, M. Li, Y. Li, X. Liu, X. Zhang, Z. Lan, J. Wang, Y. Du, S. Zhang, Zn/Sr dual ions-collagen co-assembly hydroxyapatite enhances bone regeneration through procedural osteo-immunomodulation and osteogenesis. Bioact. Mater. 10, 195 (2022). https://doi.org/10.1016/j.bioactmat.2021.09.01310.1016/j.bioactmat.2021.09.013 ↗
90. Y. Song, H. Wu, Y. Gao, J. Li, K. Lin, B. Liu, X. Lei, P. Cheng, S. Zhang, Y. Wang, J. Sun, L. Bi, G. Pei, Zinc silicate/nano-hydroxyapatite/collagen scaffolds promote angiogenesis and bone regeneration via the p38 MAPK pathway in activated monocytes. ACS Appl. Mater. Interfaces 12(14), 16058 (2020). https://doi.org/10.1021/acsami.0c0047010.1021/acsami.0c00470 ↗
91. H. Begam, S.K. Nandi, A. Chanda, B. Kundu, Effect of bone morphogenetic protein on Zn-HAp and Zn-HAp/collagen composite: a systematic in vivo study. Res. Vet. Sci. 115, 1 (2017). https://doi.org/10.1016/j.rvsc.2017.01.01210.1016/j.rvsc.2017.01.012 ↗