The potential of the mineralized bone allograft block as an appropriate candidate for bone tissue engineering in periodontology
Source abstract
Abstract
In this research, an allograft mineralized bone block was processed through decellularizing and lyophilizing after approving the sterility evaluations of the donor. According to SEM images, the block was an interconnected porous structure with pore sizes ranging between 124 and 930 µm which comprised various sizes desirable for bone regeneration. Also, emergence of hydroxyapatite crystals on the scaffold after immersing in simulated body fluid indicated its bioactivity. Furthermore, its mechanical strength was 33.10 ± 0.34 MPa which is suitable for a bone scaffold as load-bearing tissue. The successful decellularization was approved by hematoxylin–eosin staining assay and the scaffold exhibited no cytotoxicity utilizing MTT test. Furthermore, the obtained SEM images after cell seeding as well as the DAPI staining results demonstrated the supporting of cell attachment and proliferation by the scaffold. Therefore, it could be concluded that the prepared allograft mineralized bone block is an appropriate candidate for bone tissue regeneration in periodontology.Graphical abstractThe process of mineralized bone allograft block production and characterization.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Electrospun Nanofibers in Biomedical Applications · Tissue Engineering and Regenerative Medicine
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (68)
Show source-provided references
1. P. Wang, L. Zhao, J. Liu, M.D. Weir, X. Zhou, H.H. Xu, Bone tissue engineering via nanostructured calcium phosphate biomaterials and stem cells. Bone Res. 2(1), 1 (2014)10.1038/boneres.2014.17 ↗
2. A. Bigham-Sadegh and A. Oryan: Basic concepts regarding fracture healing and the current options and future directions in managing bone fractures(1742–481X (Electronic)).
3. H.-S. Sohn, J.-K. Oh, Review of bone graft and bone substitutes with an emphasis on fracture surgeries. Biomater. Res. 23(1), 9 (2019)10.1186/s40824-019-0157-y ↗
4. J.D. Cooper, T.J. Dekker, J.J. Ruzbarsky, L.A. Pierpoint, R.W. Soares, M.J. Philippon, Autograft versus allograft: the evidence in hip labral reconstruction and augmentation. Am. J. Sports Med. 49(13), 3575 (2021)10.1177/03635465211042633 ↗
5. H. Tebyanian, M.H. Norahan, H. Eyni, M. Movahedin, S.J. Mortazavi, A. Karami, M.R. Nourani, N. Baheiraei, Effects of collagen/β-tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects. J. Appl. Biomater. Funct. Mater. 17(1), 2280800018820490 (2019)
6. M. Meskinfam, S. Bertoldi, N. Albanese, A. Cerri, M.C. Tanzi, R. Imani, N. Baheiraei, M. Farokhi and S. Farè: Polyurethane foam/nano hydroxyapatite composite as a suitable scaffold for bone tissue regeneration(1873–0191 (Electronic)).
7. W. Wang and K.W.K. A. Neishabouri, A. Soltani Khaboushan, F. Daghigh, A.-M. Kajbafzadeh and M. Majidi Zolbin: A review (2452–199X (Electronic)).
8. T. Lu, B. Yang, R. Wang, C. Qin, Xenotransplantation: current status in preclinical research. Front. Immunol. (2020). https://doi.org/10.3389/fimmu.2019.0306010.3389/fimmu.2019.03060 ↗
9. A. Neishabouri, A. Soltani Khaboushan, F. Daghigh, A.-M. Kajbafzadeh and M. Majidi Zolbin: Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods. Frontiers in Bioengineering and Biotechnology 10, (2022).10.3389/fbioe.2022.805299 ↗
10. H.A.-O. Amirazad, M.A.-O. Dadashpour and N.A.-O. Zarghami: Application of decellularized bone matrix as a bioscaffold in bone tissue engineering(1754–1611 (Print)).
11. E. Gruskin, B.A. Doll, F.W. Futrell, J.P. Schmitz, J.O. Hollinger, Demineralized bone matrix in bone repair: history and use. Adv. Drug Deliv. Rev. 64(12), 1063 (2012)10.1016/j.addr.2012.06.008 ↗
12. N.A. Ebraheim, H. Elgafy, R. Xu, Bone-graft harvesting from iliac and fibular donor sites: techniques and complications. JAAOS-J. Am. Acad. Orthopaedic Surg. 9(3), 210 (2001)10.5435/00124635-200105000-00007 ↗
13. Z. Amini, R. Lari, A systematic review of decellularized allograft and xenograft-derived scaffolds in bone tissue regeneration. Tissue Cell 69, 101494 (2021)10.1016/j.tice.2021.101494 ↗
14. B. McEvoy, N.J. Rowan, Terminal sterilization of medical devices using vaporized hydrogen peroxide: a review of current methods and emerging opportunities. J. Appl. Microbiol. 127(5), 1403 (2019)10.1111/jam.14412 ↗
15. J.M. Wagner, N. Conze, G. Lewik, C. Wallner, J.C. Brune, S. Dittfeld, H. Jaurich, M. Becerikli, M. Dadras, K. Harati, Bone allografts combined with adipose-derived stem cells in an optimized cell/volume ratio showed enhanced osteogenesis and angiogenesis in a murine femur defect model. J. Mol. Med. 97, 1439 (2019)10.1007/s00109-019-01822-9 ↗
16. D.M. Strong, G.E. Friedlaender, W.W. Tomford, D.S. Springfield, T.C. Shives, H. Burchardt, W. Enneking, H.J. Mankin, Immunologic responses in human recipients of osseous and osteochondral allografts. Clin. Orthopaedics Related Res. 326, 107 (1996)10.1097/00003086-199605000-00013 ↗
17. D.M. Ehrler, A.R. Vaccaro, The use of allograft bone in lumbar spine surgery. Clin. Orthopaedics Related Res. 371, 38 (2000)10.1097/00003086-200002000-00005 ↗
18. A. Kaminski, A. Jastrzebska, E. Grazka, J. Marowska, G. Gut, A. Wojciechowski, I. Uhrynowska-Tyszkiewicz, Effect of gamma irradiation on mechanical properties of human cortical bone: influence of different processing methods. Cell Tissue Banking 13, 363 (2012)10.1007/s10561-012-9308-2 ↗
19. W. Wei, Y. Liu, X. Yang, S. Tian, C. Liu, Y. Zhang, Z. Xu, B. Hu, Z. Tian, K. Sun, Fractionation of 50 kGy electron beam irradiation: effects on biomechanics of human flexor digitorum superficialis tendons treated with ascorbate. J. Biomech. 46(4), 658 (2013)10.1016/j.jbiomech.2012.11.049 ↗
20. A. Pruss, M. Kao, U. Gohs, J. Koscielny, R. von Versen, G. Pauli, Effect of gamma irradiation on human cortical bone transplants contaminated with enveloped and non-enveloped viruses. Biologicals 30(2), 125 (2002)10.1006/biol.2002.0326 ↗
21. S. Naishlos, E. Zenziper, H. Zelikman, J. Nissan, S. Mizrahi, G. Chaushu, S. Matalon, L. Chaushu, Esthetic assessment succeeding anterior atrophic maxilla augmentation with cancellous bone-block allograft and late restoration loading. J Clin. Med. 10(20), 4635 (2021)10.3390/jcm10204635 ↗
22. S.R. Motamedian, M. Khojaste, A. Khojasteh, Success rate of implants placed in autogenous bone blocks versus allogenic bone blocks: a systematic literature review. Annals Maxillofacial Surg. 6(1), 78 (2016)10.4103/2231-0746.186143 ↗
23. T.W. Sterio, J.A. Katancik, S.B. Blanchard, P. Xenoudi, B.L. Mealey, A prospective, multicenter study of bovine pericardium membrane with cancellous particulate allograft for localized alveolar ridge augmentation. Int. J. Periodontics Restor. Dent. (2013). https://doi.org/10.11607/prd.170410.11607/prd.1704 ↗
24. H. Mohammadi, M. Sepantafar, N. Muhamad, A. Bakar Sulong, How does scaffold porosity conduct bone tissue regeneration. Adv. Eng. Mater. 23(10), 2100463 (2021)10.1002/adem.202100463 ↗
25. S. Tabatabaee, N. Baheiraei, M. Salehnia, Fabrication and characterization of PHEMA–gelatin scaffold enriched with graphene oxide for bone tissue engineering. J. Orthopaedic Surg. Res. 17(1), 216 (2022)10.1186/s13018-022-03122-4 ↗
26. S.G. González, M.D. Vlad, J.L. López, E.F. Aguado, Novel bio-inspired 3D porous scaffold intended for bone-tissue engineering: Design and in silico characterisation of histomorphometric, mechanical and mass-transport properties. Mater. Design 225, 111467 (2023)10.1016/j.matdes.2022.111467 ↗
27. C.M. Murphy and F.J. O'Brien: Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds(1933–6926 (Electronic)).
28. J. Jiao, Q. Hong, D. Zhang, M. Wang, H. Tang, J. Yang, X. Qu and B. Yue: Influence of porosity on osteogenesis, bone growth and osteointegration in trabecular tantalum scaffolds fabricated by additive manufacturing Frontiers in Bioengineering and Biotechnology. 11, (2023).10.3389/fbioe.2023.1117954 ↗
29. E. Novitskaya, S. Chen Py Fau - Lee, A. Lee S Fau - Castro-Ceseña, G. Castro-Ceseña A Fau - Hirata, V.A. Hirata G Fau - Lubarda, J. Lubarda Va Fau - McKittrick and J. McKittrick: Anisotropy in the compressive mechanical properties of bovine cortical bone and the mineral and protein constituents(1878–7568 (Electronic)).
30. S. Bose, S. Tarafder, Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering: a review. Acta Biomaterialia 8(4), 1401 (2012)10.1016/j.actbio.2011.11.017 ↗
31. B. Ben-Nissan, Advances in calcium phosphate biomaterials (Springer, Berlin, 2014)10.1007/978-3-642-53980-0 ↗
32. E. Fujii, M. Ohkubo, K. Tsuru, S. Hayakawa, A. Osaka, K. Kawabata, C. Bonhomme, F. Babonneau, Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite. Acta Biomaterialia 2(1), 69 (2006)10.1016/j.actbio.2005.09.002 ↗
33. T. Komori, Regulation of osteoblast differentiation by Runx2, in Osteoimmunology: Interactions of the Immune and skeletal systems II (Springer, Boston, 2010), p.43
34. C.-H. Lin, Y.-S. Chen, W.-L. Huang, T.-C. Hung, T.-C. Wen, Hydroxyapatite formation with the interface of chitin and chitosan. J. Taiwan Inst. Chem. Eng. 118, 294 (2021)10.1016/j.jtice.2021.01.004 ↗
35. A. Ullah, M.K. Haider, F.-F. Wang, S. Morita, D. Kharaghani, Y. Ge, Y. Yoshiko, J.S. Lee, I.S. Kim, “Clay-corn-caprolactone” a novel bioactive clay polymer nanofibrous scaffold for bone tissue engineering. Appl. Clay Sci. 220, 106455 (2022)10.1016/j.clay.2022.106455 ↗
36. J. Jeong, J.H. Kim, J.H. Shim, N.S. Hwang, C.Y. Heo, Bioactive calcium phosphate materials and applications in bone regeneration. Biomater. Res. 23(1), 1 (2019)10.1186/s40824-018-0153-7 ↗
37. J.H. Cole and M.C. van der Meulen: Whole bone mechanics and bone quality(1528–1132 (Electronic)).
38. S.K. Venkatraman, R. Choudhary, N. Vijayakumar, G. Krishnamurithy, H.R.B. Raghavendran, M.R. Murali, T. Kamarul, A. Suresh, J. Abraham, S. Swamiappan, Investigation on bioactivity, mechanical stability, bactericidal activity and in-vitro biocompatibility of magnesium silicates for bone tissue engineering applications. J. Mater. Res. (2022). https://doi.org/10.1557/s43578-021-00450-910.1557/s43578-021-00450-9 ↗
39. F.A. Sabet, A. Raeisi Najafi, E. Hamed and I. Jasiuk: Modelling of bone fracture and strength at different length scales: a review(2042–8898 (Print)).
40. M. Figueiredo, J. Gamelas, A. Martins, Characterization of bone and bone-based graft materials using FTIR spectroscopy. Infrared Spectrosc-life Biomed. Sci. (2012). https://doi.org/10.5772/3637910.5772/36379 ↗
41. H. Follet, G. Boivin, C. Rumelhart, P.J. Meunier, The degree of mineralization is a determinant of bone strength: a study on human calcanei. Bone 34(5), 783 (2004)10.1016/j.bone.2003.12.012 ↗
42. L. Wang, J. Kang, C. Sun, D. Li, Y. Cao, Z. Jin, Mapping porous microstructures to yield desired mechanical properties for application in 3D printed bone scaffolds and orthopaedic implants. Mater. Design 133, 62 (2017)10.1016/j.matdes.2017.07.021 ↗
43. E.G. Choubar, M.H. Nasirtabrizi, F. Salimi, N. Sohrabi-gilani, A. Sadeghianamryan, Fabrication and in vitro characterization of novel co-electrospun polycaprolactone/collagen/polyvinylpyrrolidone nanofibrous scaffolds for bone tissue engineering applications. J. Mater. Res. (2022). https://doi.org/10.1557/s43578-022-00778-w10.1557/s43578-022-00778-w ↗
44. S. Mohammadi, S.S. Shafiei, M. Asadi-Eydivand, M. Ardeshir, M. Solati-Hashjin, Graphene oxide-enriched poly (ε-caprolactone) electrospun nanocomposite scaffold for bone tissue engineering applications. J. Bioactive and Compatible Polym. 32(3), 325 (2017)10.1177/0883911516668666 ↗
45. L. Zhang, K. Pan, S. Huang, X. Zhang, X. Zhu, Y. He, X. Chen, Y. Tang, L. Yuan, D. Yu, Graphdiyne oxide-mediated photodynamic therapy boosts enhancive T-cell immune responses by increasing cellular stiffness. Int. J. Nanomed. (2023). https://doi.org/10.2147/IJN.S39299810.2147/IJN.S392998 ↗
46. C. Sandino, D. Lacroix, A dynamical study of the mechanical stimuli and tissue differentiation within a CaP scaffold based on micro-CT finite element models. Biomech Model Mech. 10, 565 (2011)10.1007/s10237-010-0256-0 ↗
47. D. Rowley: Bone repair biomaterials: JA Planell Pp. 478. New Delhi: Woodhead Publishing Ltd, 2009. ISBN: 978-1-84569-385-5.£ 145.00, (The British Editorial Society of Bone and Joint Surgery, City, 2010).
48. S.A.-O. Chu, H. Xu, X. Li, T. Guo, Z. Ting and Y. Zhou: Application of modified alveolar ridge augmentation technique for horizontal bone augmentation in posterior mandibular region: Report of 3 cases(2050-0904 (Print)).
49. A. Manke, L. Wang, Y. Rojanasakul, Mechanisms of nanoparticle-induced oxidative stress and toxicity. BioMed Res. Int. (2013). https://doi.org/10.1155/2013/94291610.1155/2013/942916 ↗
50. G. Turnbull, J. Clarke, F. Picard, P. Riches, L. Jia, F. Han, B. Li and W. Shu: 3D bioactive composite scaffolds for bone tissue engineering(2452-199X (Electronic)).10.1016/j.bioactmat.2017.10.001 ↗
51. G. Zhu, T. Zhang, M. Chen, K. Yao, X. Huang, B. Zhang, Y. Li, J. Liu, Y. Wang, Z. Zhao, Bone physiological microenvironment and healing mechanism: Basis for future bone-tissue engineering scaffolds. Bioactive Mater. 6(11), 4110 (2021)10.1016/j.bioactmat.2021.03.043 ↗
52. P. Feng, P. Wei, P. Li, C. Gao, C. Shuai, S. Peng, Calcium silicate ceramic scaffolds toughened with hydroxyapatite whiskers for bone tissue engineering. Mater. Character. 97, 47 (2014)10.1016/j.matchar.2014.08.017 ↗
53. X. Wu, K. Walsh, B.L. Hoff, G. Camci-Unal, Mineralization of biomaterials for bone tissue engineering. Bioengineering 7(4), 132 (2020)10.3390/bioengineering7040132 ↗
54. E. Steijvers, A. Ghei and Z. Xia: Manufacturing artificial bone allografts: a perspective(2096-112X (Electronic)).
55. B.B. Rothrauff, R.S. Tuan, Decellularized bone extracellular matrix in skeletal tissue engineering. Biochem. Soc. Trans. 48(3), 755 (2020)10.1042/BST20190079 ↗
56. G. Chen, Y. Lv, Decellularized bone matrix scaffold for bone regeneration. Decellularized scaffolds organogenesis Meth Proto (2018). https://doi.org/10.1007/7651_2017_5010.1007/7651_2017_50 ↗
57. K.E. Benders, P.R. Van Weeren, S.F. Badylak, D.B. Saris, W.J. Dhert, J. Malda, Extracellular matrix scaffolds for cartilage and bone regeneration. Trends Biotechnol. 31(3), 169 (2013)10.1016/j.tibtech.2012.12.004 ↗
58. A. Pintor, L. Queiroz, R. Barcelos, L. Primo, L. Maia, G. Alves, MTT versus other cell viability assays to evaluate the biocompatibility of root canal filling materials: a systematic review. Int. Endodontic J. 53(10), 1348 (2020)10.1111/iej.13353 ↗
59. F. Kouhestani, F. Dehabadi, M. Hasan Shahriari, S.R. Motamedian, Allogenic vs. synthetic granules for bone tissue engineering: an in vitro study. Prog. Biomater. 7, 133 (2018)10.1007/s40204-018-0092-3 ↗
60. F. Barrere, T. Mahmood, K. De Groot, C. Van Blitterswijk, Advanced biomaterials for skeletal tissue regeneration: instructive and smart functions. Mater. Sci. Eng. R: Rep. 59(1–6), 38 (2008)10.1016/j.mser.2007.12.001 ↗
61. R.A. Perez, G. Mestres, Role of pore size and morphology in musculo-skeletal tissue regeneration. Mater. Sci. Eng: C 61, 922 (2016)10.1016/j.msec.2015.12.087 ↗
62. J. Zeltinger, J.K. Sherwood, D.A. Graham, R. Müeller, L.G. Griffith, Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. Tissue Eng. 7(5), 557 (2001)10.1089/107632701753213183 ↗
63. Y. Kuboki, Q. Jin, H. Takita, Geometry of carriers controlling phenotypic expression in BMP-induced osteogenesis and chondrogenesis. JBJS 83(1), S105 (2001)
64. T.I. Shaheen, A. Montaser, S. Li, Effect of cellulose nanocrystals on scaffolds comprising chitosan, alginate and hydroxyapatite for bone tissue engineering. Int. J. Biol. Macromol. 121, 814 (2019)10.1016/j.ijbiomac.2018.10.081 ↗
65. S. Bhumiratana, W.L. Grayson, A. Castaneda, D.N. Rockwood, E.S. Gil, D.L. Kaplan, G. Vunjak-Novakovic, Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds. Biomaterials 32(11), 2812 (2011)10.1016/j.biomaterials.2010.12.058 ↗
66. S. Samavedi, A.R. Whittington, A.S. Goldstein, Calcium phosphate ceramics in bone tissue engineering: a review of properties and their influence on cell behavior. Acta Biomaterialia 9(9), 8037 (2013)10.1016/j.actbio.2013.06.014 ↗
67. J. Ryu, S.H. Ku, H. Lee, C.B. Park, Mussel-inspired polydopamine coating as a universal route to hydroxyapatite crystallization. Adv. Funct. Mater. 20(13), 2132 (2010)10.1002/adfm.200902347 ↗
68. H.-J. Park, J.-H. Choi, M.-H. Nam, Y.-K. Seo, Induced neurodifferentiation of hBM-MSCs through activation of the ERK/CREB pathway via pulsed electromagnetic fields and physical stimulation promotes neurogenesis in cerebral ischemic models. Int. J. Mol. Sci. 23(3), 1177 (2022)10.3390/ijms23031177 ↗