Development, characterization and antimicrobial properties of silver nanoparticles loaded chitosan-alginate sponges for biomedical application
Source abstract
Abstract
In this study chitosan/alginate (Ch/Alg) composite scaffolds were prepared using a freeze-drying method and loaded with various concentrations of silver nanoparticles (AgNPs). Structural, chemichal composition as well as biocompatibility and antibacterial properties were studied focused to biomedical application. Formation of polyelectrolyte complexes between chitosan and sodium alginate increases the mechanical resistance of the scaffold as well as porous morphology of the sample which is crucial for cell culture applications and results in the formation of a material characterized by good structural integrity. As-cast materials demonstrate high biocompatibility profile but AgNPs-loaded scaffold demonstrate minor cell inhibitory potencial. The early silver ion release during material degradation can affect cell viability, cell adhesion and proliferation. AgNPs in concentration of 6 × 10−5 g/mL significantly improves the antibacterial effect of sponges Alg/Ch/NaHCO3 (2:2:1), Alg/Ch/NaHCO3 (1:3:1) and Ch/NaHCO3 (1:1) against Gram-negative bacteria.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanocomposite Films for Food Packaging · Nanoparticles: synthesis and applications · Polymer Surface Interaction Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (33)
Show source-provided references
1. M.N. Pastore, Y.N. Kalia, M. Horstmann, M.S. Roberts, Transdermal patches: history, development and pharmacology. Review. Br. J. Pharmacol. 172, 2179–2209 (2015). https://doi.org/10.1111/bph.1305910.1111/bph.13059 ↗
2. J. Radwan-Pragłowska, M. Piatkowski, V. Deineka, Ł Janus, V. Korniienko, E. Husak, V. Holubnycha, I. Liubchak, V. Zhurba, A. Sierakowska, M. Pogorielov, D. Bogdał, Chitosan-based bioactive hemostatic agents with antibacterial properties—synthesisand characterization. Molecules 24, 2629 (2019). https://doi.org/10.3390/molecules2414262910.3390/molecules24142629 ↗
3. S.C. Mellinghoff, C. Otto, O.A. Cornely, Surgical site infections: current management and role of new antibiotics. Curr. Opin. Infect. Dis. 32(5), 517–522 (2019). https://doi.org/10.1097/QCO.000000000000058910.1097/QCO.0000000000000589 ↗
4. C. Elliott, A. Justiz-Vaillant, Nosocomial infections: a 360-degree review. Int. Biol. Biomed. J. 4(2), 72–81 (2018)
5. G. Laverty, S.P. Gorman, B.F. Gilmore, Biomolecular mechanisms of Pseudomonas aeruginosa and Escherichia coli biofilm formation. Pathogens 3(3), 596–632 (2014). https://doi.org/10.3390/pathogens303059610.3390/pathogens3030596 ↗
6. R.Y. Pelgrift, A.J. Friedman, Nanotechnology as a therapeutic tool to combat microbial resistance. Adv. Drug Deliv. Rev. 65(13–14), 1803–1815 (2013). https://doi.org/10.1016/j.addr.2013.07.01110.1016/j.addr.2013.07.011 ↗
7. K.M. Hindi, A.J. Ditto, M.J. Panzner, D.A. Medvetz, D.S. Han, C.E. Hovis, J.K. Hilliard, J.B. Taylor, Y.H. Yun, C.L. Cannon, W.J. Youngs, The antimicrobial efficacy of sustained release silver-carbene complex-loaded L-tyrosine polyphosphate nanoparticles: characterization, in vitro and in vivo studies. Biomaterials 30(22), 3771–3779 (2009). https://doi.org/10.1016/j.biomaterials.2009.03.04410.1016/j.biomaterials.2009.03.044 ↗
8. D.S. Ipe, P.T. Sudheesh Kumar, R.M. Love, S.M. Hamlet, Silver nanoparticles at biocompatible dosage synergistically increases bacterial susceptibility to antibiotics. Front. Microbiol. 11, 1074 (2020). https://doi.org/10.3389/fmicb.2020.0107410.3389/fmicb.2020.01074 ↗
9. K.T. Shalumona, N.S. Binulala, N. Selvamurugana, S.V. Naira, D. Menona, T. Furuikeb, H. Tamurab, R. Jayakumara, Electrospinning of carboxymethyl chitin/poly(vinyl alcohol) nanofibrous scaffolds for tissue engineering applications. Carbohydr. Polym. 77(4), 863–869 (2009). https://doi.org/10.1016/j.carbpol.2009.03.00910.1016/j.carbpol.2009.03.009 ↗
10. K. Sun, Z.H. Li, Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning. Express Polym. Lett. 5(4), 342–336 (2011). https://doi.org/10.3144/expresspolymlett.2011.310.3144/expresspolymlett.2011.3 ↗
11. E. Szymańska, K. Winnicka, P. Laurienzo, Stability of Chitosan—a challenge for pharmaceutical and biomedical applications. Mar. Drugs 13(4), 1819–1846 (2015). https://doi.org/10.3390/md1304181910.3390/md13041819 ↗
12. H. Yan, X. Chen, M. Feng, Z. Shi, D. Zhang, Q. Lin, Layer-by-layer assembly of 3D alginate-chitosan-gelatin composite scaffold incorporating bacterial cellulose nanocrystals for bone tissue engineering. Mater. Lett. 209, 492–496 (2017)10.1016/j.matlet.2017.08.093 ↗
13. G. Zheng, X. Liu, X. Wang, L. Chen, H. Xie, F. Wang et al., Improving stability and biocompatibility of alginate/chitosan microcapsule by fabricating Bi-functional membrane. J. Macromol. Biosci. 14(5), 655–666 (2014)10.1002/mabi.201300474 ↗
14. H. Amir Afshar, A. Ghaee, Preparation of aminated chitosan/alginate scaffold containing halloysite nanotubes with improved cell attachment. Carbohydr. Polym. 151, 1120–1131 (2016)10.1016/j.carbpol.2016.06.063 ↗
15. A.M. Banoi, T. Yasin, M.A. Ghauri, M. Younus, Chitosan: a potential biopolymer for wound management. Int. J. Biol. Macromol. 102, 380–383 (2017)10.1016/j.ijbiomac.2017.04.047 ↗
16. S. Nokhasteh, A.M. Molavi, M. Khorsand-Ghayeni, A. Sadeghi-Avalshahr, Preparation of PVA/Chitosan samples by electrospinning and film casting methods and evaluating the effect of surface morphology on their antibacterial behavior. Mater. Res. Express 7, 015401 (2020)10.1088/2053-1591/ab572c ↗
17. A. Yanovska, V. Kuznetsov, A. Stanislavov, E. Husak, M. Pogorielov, V. Starikov, S. Bolshanina, S. Danilchenko, Synthesis and characterization of hydroxyapatite-gelatine composite materials for orthopaedic application. Mater. Chem. Phys. 183, 93–100 (2016)10.1016/j.matchemphys.2016.08.006 ↗
18. Y. Qing, L. Cheng, R. Li, G. Liu, Y. Zhang, X. Tang, J. Wang, H. Liu, Y. Qin, Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies. Int. J. Nanomed. 13, 3311–3327 (2018)10.2147/IJN.S165125 ↗
19. S. Jin, J. Li, J. Wang, J. Jiang, Y. Zuo, Y. Li, F. Yang, Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration. Int. J. Nanomed. 13, 4591–4605 (2018)10.2147/IJN.S167793 ↗
20. O. Oleshko, I. Liubchak, Y. Husak, V. Korniienko, A. Yusupova, T. Oleshko, R. Banasiuk, M. Szkodo, I. Matros-Taranets, A. Kazek-Kęsik, W. Simka, M. Pogorielov, In vitro biological characterization of silver-doped anodic oxide coating on titanium. Materials 13, 4359 (2020)10.3390/ma13194359 ↗
21. Y.B. Seo, O.J. Lee, Md.T. Sultan, J.M. Lee, Y.R. Park, Y.K. Yeon et al., In vitro and in vivo evaluation of the duck’s feet collagen sponge for hemostatic applications. J. Biomater. Appl. 32(4), 484–491 (2017)10.1177/0885328217733338 ↗
22. S. Castro-Gamboa, M.R. Garcia-Garcia, G. Piñon- Zarate, M. Rojas-Lemus, K. Jarquin-Yañez, M.A. Herrera-Enriquez, T.I. Fortoul, Y. Toledano-Magaña, T. Garcia-Iglesias, A. Pestryakov, A.S. Eliu Castell-Rodriguez, N. Bogdanchikova, Toxicity of silver nanoparticles in mouse bone marrow- derived dendritic cells: Implications for phenotype. J. Immunotoxicol. 16(1), 54–62 (2019). https://doi.org/10.1080/1547691X.2019.158465210.1080/1547691X.2019.1584652 ↗
23. S. Sharma, P. Sanpui, A. Chattopadhyay, S.S. Ghosh, Fabrication of antibacterial silver nanoparticle-sodium alginate–chitosan composite films. RSC Adv. 2, 5837–5843 (2012). https://doi.org/10.1039/C2RA00006G10.1039/C2RA00006G ↗
24. M.M. Said, M. Rehan, S.M. El-Sheikh, M.K. Zahran, M.S. Abdel-Aziz, M. Bechelany, A. Barhoum, Multifunctional hydroxyapatite/silver nanoparticles/cotton gauze for antimicrobial and biomedical applications. Nanomaterials 11(2), 429 (2021). https://doi.org/10.3390/nano1102042910.3390/nano11020429 ↗
25. M. Nasr, L. Soussan, R. Viter, C. Eid, R. Habchi, P. Mielead, M. Bechelany, High photodegradation and antibacterial activity of BN–Ag/TiO2 composite nanofibers under visible light. New J. Chem. 42, 1250–1259 (2018)10.1039/C7NJ03183A ↗
26. S. Radhakrishnan, S. Nagarajan, H. Belaid, C. Farha, I. Iatsunskyi, E. Coy, L. Soussan, V. Huon, J. Bares, K. Belkacemi, C. Teyssier, S. Balme, P. Miele, D. Cornu, N. Kalkura, V. Cavaillès, M. Bechelany, Fabrication of 3D printed antimicrobial polycaprolactone scaffolds for tissue engineering applications. Mater. Sci. Eng. C 118, 111525 (2021). https://doi.org/10.1016/j.msec.2020.11152510.1016/j.msec.2020.111525 ↗
27. X. Zhao, Q. Li, X. Li, Antibacterial activity and in vitro cytotoxicity evaluation of alginate-AgNP fibers. Text. Res. J. 87(11), 1377–1386 (2016)10.1177/0040517516652350 ↗
28. B. Erdem, S. Kurt Mand Okur, Morphological analysis of the antibacterial action of chitosan on gram-negative bacteria using atomic force microscopy. Curr. Opin. Biotechnol. 24, S83 (2013)10.1016/j.copbio.2013.05.240 ↗
29. A. Kubyshkin, D. Chegodar, A. Katsev, A. Petrosyan, Y. Krivorutchenko, O. Postnikova, Antimicrobial effects of silver nanoparticles stabilized in solution by sodium alginate. Biochem. Mol. Biol. J. (2016). https://doi.org/10.21767/2471-8084.10002210.21767/2471-8084.100022 ↗
30. L.F. Gómez Chabala, C.E. Echeverri Cuartas, M.E. Londoño López, Release behavior and antibacterial activity of chitosan/alginate blends with aloe vera and silver nanoparticles. Mar. Drugs 15(10), 328 (2017). https://doi.org/10.3390/md1510032810.3390/md15100328 ↗
31. M. Arkoun, F. Daigle, M.-C. Heuzey, A. Ajji, Mechanism of action of electrospun chitosan-based nanofibers against meat spoilage and pathogenic bacteria. Molecules 22(4), 585 (2017). https://doi.org/10.3390/molecules2204058510.3390/molecules22040585 ↗
32. Y. Tao, L.-H. Qian, J. Xie, Effect of chitosan on membrane permeability and cell morphology of Pseudomonas aeruginosa and Staphyloccocus aureus. Carbohydr. Polym. 86(2), 969–974 (2011)10.1016/j.carbpol.2011.05.054 ↗
33. T. Takahashi, M. Imai, I. Suzuki, J. Sawai, Growth inhibitory effect on bacteria of chitosan membranes regulated with deacetylation degree. Biochem. Eng. J. 40(3), 485–491 (2008)10.1016/j.bej.2008.02.009 ↗