journal article·2025-11-28

Facile synthesis of zinc oxide nanoparticles for skin care applications

Sharad Kumar Tripathi, Ajay Kumar Tiwari, Rajesh Kumar, Abhishek Kumar Bhardwaj, Ram Raseele Awasthi, Anand Pandey, Afifa Qidwai, Anupam Dikshit

Journal of Materials Research· Volume 40· Issue 22
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Abstract

Scientists are focused on creating skincare solutions to combat various factors like pollution, UV rays, and skin infections. In the present investigation, a wet chemical method was employed to synthesize the zinc oxide (ZnO) nanoparticles (NPs). The physicochemical properties of the synthesized material were studied, including X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and ultraviolet–visible spectroscopy (UV–Vis) techniques. In addition to it, the synthesized material was tested against four pathogens:Trichophyton mentagrophytes,Trichophyton rubrum,Microsporum gypseum, andMicrosporum canis. The minimal inhibitory concentrations (MICs) of the synthesized material against fungal pathogens were recorded as 0.711, 2.469, 0.786, and 1.789 mg/ml, respectively, which almost effectively suppressed over 80% of their growth. Its worth noting that the properties of nanomaterials can be tailored by adjusting their surface, shape, and size. These findings suggest that ZnO NPs hold promise as effective fungicidal compounds.Graphical abstract
Source: expertdata · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Nanoparticles: synthesis and applications · Dielectric properties of ceramics · TiO2 Photocatalysis and Solar Cells

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

References (51)

Show source-provided references
1. J.L. Xu, Y.X. Luo, S.H. Yuan, L.W. Li, N.N. Liu, Antifungal nanomaterials: current progress and future directions. Innov. Digit. Health Diagn. Biomark. 1(1), 3–7 (2021). https://doi.org/10.36401/IDDB-20-0310.36401/IDDB-20-03 ↗
2. A.K. Gupta, T. Wang, T.M. Susmita, W.L. Bakotic, Global dermatophyte infections linked to human and animal health: a scoping review. Microorganisms. 13(3), 575 (2025). https://doi.org/10.3390/microorganisms1303057510.3390/microorganisms13030575 ↗
3. Q. Li, J. Li, H. Zhi, W. Lv, B. Sang, Y. Zhong, X. Chen, X. Xia, Z. Liu, Epidemiological survey of 32,786 culture-positive dermatophytosis cases in Hangzhou from 2018 to 2023. Mycopathologia 189(6), 98 (2024). https://doi.org/10.1007/s11046-024-00899-210.1007/s11046-024-00899-2 ↗
4. A.K. Gupta, S.P. Ravi, T. Wang, S. Faour, M.A. Bamimore, C.R. Heath, S.F. Friedlander, An update on tinea capitis in children. Pediatr. Dermatol. 41(6), 1030–1039 (2024). https://doi.org/10.1111/pde.1570810.1111/pde.15708 ↗
5. L.W. Wei, J.J. Qiao, Mini-review: the diagnostic methods of tinea capitis. Mycopathologia 188(5), 563–569 (2023). https://doi.org/10.1007/s11046-023-00731-310.1007/s11046-023-00731-3 ↗
6. X.Q. Chen, D.Y. Zheng, Y.Y. Xiao, B.L. Dong, C.W. Cao, L. Ma, Z.S. Tong, M. Zhu, Z.H. Liu, L.Y. Xi, M. Fu, Aetiology of tinea capitis in China: a multicentre prospective study. Br. J. Dermatol. 186(4), 705–712 (2022). https://doi.org/10.1111/bjd.2087510.1111/bjd.20875 ↗
7. M. Ravichandran, S. Srikanth, B.A. Kumar, R. Munusamy, Efficacy and safety of eberconazole vs sertaconazole in localised tinea infection. Indian J. Dermatol. 66(5), 573 (2021). https://doi.org/10.4103/ijd.IJD_893_2010.4103/ijd.IJD_893_20 ↗
8. M. Gholami, F. Mokhtari, R. Mohammadi, Identification of Malassezia species using direct PCR-sequencing on clinical samples from patients with pityriasis versicolor and seborrheic dermatitis. Curr. Med. Mycol. 6(3), 21 (2020). https://doi.org/10.18502/cmm.6.3.398410.18502/cmm.6.3.3984 ↗
9. A.K. Tiwari, S. Jha, S.K. Tripathi, R.R. Awasthi, A.K. Bhardwaj, A.K. Singh, A. Dikshit, Antioxidant response of Calendula officinalis L. assisted synthesized zinc oxide nanoparticles. Mater. Res. Express 11(8), 085005 (2024). https://doi.org/10.1088/2053-1591/ad6ca910.1088/2053-1591/ad6ca9 ↗
10. A.K. Tiwari, S. Jha, R.R. Awasthi, A.K. Singh, A.K. Bhardwaj, S.K. Tripathi, Recent trends of Zinc oxide nanoparticles for emerging biomedical application. (2024). https://doi.org/10.5772/intechopen.100765310.5772/intechopen.1007653 ↗
11. M. Shukla, S. Narain, A. Kumar, A. Dikshit, Dermal delivery of nano formulations: an emerging technology in dermatological conditions. BioNano Sci. 15(2), 215 (2025). https://doi.org/10.1007/s12668-025-01817-610.1007/s12668-025-01817-6 ↗
12. E. Sreelekha, B. George, A. Shyam, N. Sajina, B. Mathew, A comparative study on the synthesis, characterization, and antioxidant activity of green and chemically synthesized silver nanoparticles. BioNanoScience. 11, 489–496 (2021). https://doi.org/10.1007/s12668-021-00824-710.1007/s12668-021-00824-7 ↗
13. S. Prabhu, E.K. Poulose, Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int. Nano. Lett. 2, 1–10 (2012). https://doi.org/10.1186/2228-5326-2-3210.1186/2228-5326-2-32 ↗
14. A. Khatua, A. Prasad, E. Priyadarshini, A.K. Patel, A. Naik, M. Saravanan, H. Barabadi, L. Ghosh, B. Paul, R. Paulraj, R. Meena, Emerging antineoplastic plant-based gold nanoparticle synthesis: a mechanistic exploration of their anticancer activity toward cervical cancer cells. J. Clust. Sci. 31, 1329–1340 (2020). https://doi.org/10.1007/s10876-019-01742-110.1007/s10876-019-01742-1 ↗
15. J. Pulit-Prociak, A. Staroń, P. Staroń, A. Chmielowiec-Korzeniowska, A. Drabik, L. Tymczyna, M. Banach, Preparation and of PVA-based compositions with embedded silver, copper and zinc oxide nanoparticles and assessment of their antibacterial properties. J. Nanobiotechnol. 18, 1–14 (2020). https://doi.org/10.1186/s12951-020-00702-610.1186/s12951-020-00702-6 ↗
16. M. Shukla, S. Narain, A. Kumar, A. Dikshit, Characterization of titanium dioxide nanomaterials synthesized from leaf extract of Pogostemon cablin Benth and their potential antifungal activity. Phys. Scr. 99(6), 0659d3 (2024). https://doi.org/10.1088/1402-4896/ad4dea10.1088/1402-4896/ad4dea ↗
17. A.K. Tiwari, S. Jha, A.K. Singh, S.K. Mishra, A.K. Pathak, R.P. Ojha, R.S. Yadav, A. Dikshit, Innovative investigation of zinc oxide nanoparticles used in dentistry. Crystals 12(8), 1063 (2022). https://doi.org/10.3390/cryst1208106310.3390/cryst12081063 ↗
18. A.K. Tiwari, S. Jha, S.K. Tripathi, R. Shukla, R.R. Awasthi, A.K. Bhardwaj, A.K. Singh, A. Dikshit, Spectroscopic investigations of green synthesized zinc oxide nanoparticles (ZnO NPs): antioxidant and antibacterial activity. Discover Appl. Sci. 6(8), 399 (2024). https://doi.org/10.1007/s42452-024-06049-z10.1007/s42452-024-06049-z ↗
19. M.L.M. Napi, S.M. Sultan, R. Ismail, K.W. How, M.K. Ahmad, Electrochemical-based biosensors on different zinc oxide nanostructures: a review. Materials 12(18), 2985 (2019). https://doi.org/10.3390/ma1218298510.3390/ma12182985 ↗
20. S. Djearamane, L.J. Xiu, L.S. Wong, R. Rajamani, D. Bharathi, S. Kayarohanam, A.E. De Cruz, L.H. Tey, A.K. Janakiraman, M. Aminuzzaman, S. Selvaraj, Antifungal properties of zinc oxide nanoparticles on Candida albicans. Coatings 12(12), 1864 (2022). https://doi.org/10.3390/coatings1212186410.3390/coatings12121864 ↗
21. A.K. Tiwari, S. Jha, R. Agrawal, S.K. Mishra, A.K. Pathak, A.K. Singh, A. Dikshit, R.R. Awasthi, Anti-bacterial efficacy of phyto-synthesized zinc oxide nanoparticles using Murraya paniculata l. leaf extract. Sciences 4(7), 464–471 (2022). https://doi.org/10.13040/IJPSR.0975-8232.13(2).864-7110.13040/IJPSR.0975-8232.13(2).864-71 ↗
22. B. Subba, G.B. Rai, R. Bhandary, P. Parajuli, N. Thapa, D.R. Kandel, S. Mulmi, S. Shrestha, S. Malla, Antifungal activity of zinc oxide nanoparticles (ZnO NPs) on Fusarium equiseti phytopathogen isolated from tomato plant in Nepal. Heliyon (2024). https://doi.org/10.1016/j.heliyon.2024.e4019810.1016/j.heliyon.2024.e40198 ↗
23. K.A. Nxumalo, J.O. Adeyemi, T.B. Leta, T.M. Pfukwa, S.N. Okafor, O.A. Fawole, Antifungal properties and molecular docking of ZnO NPs mediated using medicinal plant extracts. Sci. Rep. 14(1), 18071 (2024). https://doi.org/10.1038/s41598-024-68979-310.1038/s41598-024-68979-3 ↗
24. C. Leeyaphan, P. Punnakitikashem, B. Suiwongsa, P. Komesmuneeborirak, P. Chongtrakool, N. Kulthanachairojana, P. Limphoka, T. Hutachoke, P. Saengthong-Aram, P. Kobkurkul, S. Wongdama, Efficacy, safety, and cost-effectiveness of zinc oxide nanoparticles in Whitfield’s spirit solution for treating superficial fungal foot infections: a randomized controlled trial. Dermatol. Ther. 15(2), 351–365 (2025). https://doi.org/10.1007/s13555-025-01340-210.1007/s13555-025-01340-2 ↗
25. V.R. Lebaka, P. Ravi, M.C. Reddy, C. Thummala, T.K. Mandal, Zinc oxide nanoparticles in modern science and technology: multifunctional roles in healthcare, environmental remediation, and industry. Nanomaterials 15(10), 754 (2025). https://doi.org/10.3390/nano1510075410.3390/nano15100754 ↗
26. N.A. Hussien, M.A.E.F. Khalil, M. Schagerl, S.S. Ali, Green synthesis of zinc oxide nanoparticles as a promising nanomedicine approach for anticancer, antibacterial, and anti-inflammatory therapies. Int. J. Nanomed. (2025). https://doi.org/10.2147/IJN.S50721410.2147/IJN.S507214 ↗
27. J.L. Rodriguez-Tudela, M.C. Arendrup, F. Barchiesi, J. Bille, E. Chryssanthou, M. Cuenca-Estrella, E. Dannaoui, D.W. Denning, J.P. Donnelly, F. Dromer, W. Fegeler, EUCAST definitive document EDef 7.1: method for the determination of broth dilution MICs of antifungal agents for fermentative yeasts: Subcommittee on Antifungal Susceptibility Testing (AFST) of the ESCMID European Committee for Antimicrobial Susceptibility Testing (EUCAST)∗. Clin. Microbiol. Infect. 14(4), 398–405 (2008)10.1111/j.1469-0691.2007.01935.x ↗
28. S.S. Mydeen, R.R. Kumar, M. Kottaisamy, V.S. Vasantha, Biosynthesis of ZnO nanoparticles through extract from Prosopis juliflora plant leaf: Antibacterial activities and a new approach by rust-induced photocatalysis. J. Saudi Chem. Soc. 24(5), 393–406 (2020). https://doi.org/10.1016/j.jscs.2020.03.00310.1016/j.jscs.2020.03.003 ↗
29. S. Fatimah, R. Ragadhita, D.F. Al Husaeni, A.B.D. Nandiyanto, How to calculate crystallite size from x-ray diffraction (XRD) using Scherrer method. ASEAN J. Sci. Eng. 2(1), 65–76 (2022)10.17509/ajse.v2i1.37647 ↗
30. X. Peng, S. Palma, N.S. Fisher, S.S. Wong, Effect of morphology of ZnO nanostructures on their toxicity to marine algae. Aquat. Toxicol. 102(3–4), 186–196 (2011). https://doi.org/10.1016/j.aquatox.2011.01.01410.1016/j.aquatox.2011.01.014 ↗
31. N. Babayevska, Ł Przysiecka, I. Iatsunskyi, G. Nowaczyk, M. Jarek, E. Janiszewska, S. Jurga, ZnO size and shape effect on antibacterial activity and cytotoxicity profile. Sci. Rep. 12(1), 8148 (2022)10.1038/s41598-022-12134-3 ↗
32. A. Muhammed, T.G. Asere, T.F. Diriba, Photocatalytic and antimicrobial properties of ZnO and Mg-doped ZnO nanoparticles synthesized using Lupinus albus leaf extract. ACS Omega 9(2), 2480–2490 (2024). https://doi.org/10.1021/acsomega.3c0709310.1021/acsomega.3c07093 ↗
33. F. Wu, B.J. Harper, S.L. Harper, Comparative dissolution, uptake, and toxicity of zinc oxide particles in individual aquatic species and mixed populations. Environ. Toxicol. Chem. 38(3), 591–602 (2019). https://doi.org/10.1002/etc.434910.1002/etc.4349 ↗
34. S. Yuan, J. Huang, X. Jiang, Y. Huang, X. Zhu, Z. Cai, Environmental fate and toxicity of sunscreen-derived inorganic ultraviolet filters in aquatic environments: a review. Nanomaterials 12(4), 699 (2022). https://doi.org/10.3390/nano1204069910.3390/nano12040699 ↗
35. J.G. Parsons, M. Alcoutlabi, R.K. Dearth, Metal oxide nanoparticle toxicity in aquatic organisms: an overview of methods and mechanisms. Nano. Biointeractions Cell Organismal. Syst. Levels. (2021). https://doi.org/10.1007/978-3-030-65792-5_510.1007/978-3-030-65792-5_5 ↗
36. X. Li, Y. Xing, Y. Jiang, Y. Ding, W. Li, Antimicrobial activities of ZnO powder-coated PVC film to inactivate food pathogens. Int. J. Food Sci. Technol. 44(11), 2161–2168 (2009). https://doi.org/10.1111/j.1365-2621.2009.02055.x10.1111/j.1365-2621.2009.02055.x ↗
37. G. Applerot, A. Lipovsky, R. Dror, N. Perkas, Y. Nitzan, R. Lubart, A. Gedanken, Enhanced antibacterial activity of nanocrystalline ZnO due to increased ROS-mediated cell injury. Adv. Funct. Mater. 19(6), 842–852 (2009). https://doi.org/10.1002/adfm.20080108110.1002/adfm.200801081 ↗
38. N. Pariona, F. Paraguay-Delgado, S. Basurto-Cereceda, J.E. Morales-Mendoza, L.A. Hermida-Montero, A.I. Mtz-Enriquez, Shape-dependent antifungal activity of ZnO particles against phytopathogenic fungi. Appl. Nanosci. 10, 435–443 (2020). https://doi.org/10.1007/s13204-019-01127-w10.1007/s13204-019-01127-w ↗
39. A.A. Keller, A. Ehrens, Y. Zheng, B. Nowack, Developing trends in nanomaterials and their environmental implications. Nat. Nanotechnol. 18(8), 834–837 (2023). https://doi.org/10.1038/s41565-023-01409-z10.1038/s41565-023-01409-z ↗
40. A.R. Hussein, S.S. Zainalabden, Preparation and characterization of zinc oxide nanoparticles by co-precipitation method and evaluation of theirs antifungal activity in spore germination of dermatophytes. Am. J. Life Sci. Innov. (2024). https://doi.org/10.54536/ajlsi.v3i2.318310.54536/ajlsi.v3i2.3183 ↗
41. A.A. Al-Janabi, A.M. Bashi, Synthesis and antifungal activity of novel griseofulvin nanoparticles with zinc oxide against dermatophytic fungi: Trichophyton mentagrophytes and Trichophyton verrucosum: a primary study. Curr. Med. Mycol. (2022). https://doi.org/10.18502/cmm.8.2.1033110.18502/cmm.8.2.10331 ↗
42. H. khalaf, younis, H. A. Awadh, H. M. Younis, N. A. Jasim, & M. A. Mustafa, The effect of antifungal and AGO, and ZnO nanoparticles on Trichophyton mentagrophytes. 7(9), 392–399( 2020). https://ejmcm.com/article_2980.html
43. M. Shukla, S. Narain, S. Jha et al., Synthesis, characterization, and determination of antifungal and antioxidant activities of plant-derived zinc oxide nanoparticles. Biomass Conv. Bioref. (2025). https://doi.org/10.1007/s13399-025-06601-w10.1007/s13399-025-06601-w ↗
44. M.A. Ashour, B.T. Abd-Elhalim, Biosynthesis and biocompatibility evaluation of zinc oxide nanoparticles prepared using Priestia megaterium bacteria. Sci. Rep. 14, 4147 (2024). https://doi.org/10.1038/s41598-024-54460-810.1038/s41598-024-54460-8 ↗
45. A.K. Arora, S. Devi, V.S. Jaswal, J. Singh, M. Kinger, V.D. Gupta, Synthesis and characterization of ZnO nanoparticles. Orient. J. Chem. 30(4), 1671–1679 (2014). https://doi.org/10.13005/ojc/30042710.13005/ojc/300427 ↗
46. S. Grovern, P. Roy, Clinico-mycological profile of superficial mycosis in a hospital in North-East India. Med. J. Armed Forces India. 59(2), 114–116 (2003). https://doi.org/10.1016/S0377-1237(03)80053-910.1016/S0377-1237(03)80053-9 ↗
47. J. Das, D.K. Jha, Antidermatophytic activity of some medicinal plants, phytochemical analysis and isolation of active fractions from Piper longum. J. Pure Appl. Microbiol. 7(4), 3207–3212 (2013)
48. P. Kannan, C. Janaki, G.S. Selvi, Prevalence of dermatophytes and other fungal agents isolated from clinical samples. Indian J. Med. Microbiol. 24(3), 212–215 (2006). https://doi.org/10.1016/S0255-0857(21)02353-710.1016/S0255-0857(21)02353-7 ↗
49. R. Kumar, S.K. Shukla, A. Pandey, S.K. Srivastava, A. Dikshit, Copper oxide nanoparticles: an antidermatophytic agent for Trichophyton spp. Nanotechnol. Rev. 4(5), 401–409 (2015). https://doi.org/10.1515/ntrev-2015-001010.1515/ntrev-2015-0010 ↗
50. P. Singh, B. Das, S. Jha, C.J. Liu, R.R. Awasthi, A.K. Tiwari, S.K. Tripathi, A.K. Bhardwaj, V.K. Nautiyal, Synthesis of chromium oxide nanoparticles and tuning to optimize magnetic and bactericidal properties. J. Inorg. Organomet. Polym. Mater. (2025). https://doi.org/10.1007/s10904-025-03751-210.1007/s10904-025-03751-2 ↗
51. S.K. Tripathi, R. Singh, S. Jha, A. Pandey, A. Dikshit, Comparison of antibacterial activities of leaf extracts of Eclipta alba against Klebsiella pneumoniae. J. Emerg. Technol. Innov. Res. 5, 349–352 (2018)