Fabrication of silver and strontium co-loaded chitosan–gelatin nanocomposite coatings with balanced antibacterial and osteogenic properties
Source abstract
Abstract
To impart balanced antibacterial and osteogenic properties to titanium substrates, we prepared silver (Ag) and strontium (Sr) co-loaded chitosan–gelatin (CSG) coatings by dual-sourced electrophoretic deposition method, producing a series of Ag/Sr-CSG-modified implants. Physicochemical study confirmed that both Ag and Sr ions were deposited into the coatings successfully. The coatings showed nanostructure, and nanoparticles could be released from the coatings. In vitro antibacterial study showed that all coatings have antibacterial activity, and as the concentration of Ag increased, the corresponding antibacterial ability improved. In the cellular study, promoted osteogenic activity was observed on Ag1/Sr10 and Ag1/Sr50 groups, and this effect of Ag1/Sr50 group was more prominent. In total, the Ag1/Sr50 group had desirable balanced antibacterial and osteogenic properties. This study demonstrated that multiple elements could be co-loaded into CSG coatings with their functional effects preserved, and the antibacterial and osteogenic property could be balanced by adjusting the elements dosage.Graphical abstract
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Polymer Surface Interaction Studies · Nanoparticles: synthesis and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (54)
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1. C. Montoya, Y. Du, A.L. Gianforcaro, S. Orrego, M.B. Yang, P.I. Lelkes, On the road to smart biomaterials for bone research: definitions, concepts, advances, and outlook. Bone Res. 9(1), 1–16 (2021)10.1038/s41413-020-00131-z ↗
2. S. Todros, M. Todesco, A. Bagno, Biomaterials and their biomedical applications: from replacement to regeneration. Processes 9(11), 1949 (2021)10.3390/pr9111949 ↗
3. C. Hu, D. Ashok, D.R. Nisbet, V. Gautam, Bioinspired surface modification of orthopedic implants for bone tissue engineering. Biomaterials 219, 119366 (2019)10.1016/j.biomaterials.2019.119366 ↗
4. J.S.L. Fong, M.A. Booth, A. Rifai, K. Fox, A. Gelmi, Diamond in the rough: toward improved materials for the bone-implant interface. Adv. Healthc. Mater. 10(14), 2100007 (2021)10.1002/adhm.202100007 ↗
5. J. Quinn, R. McFadden, C.-W. Chan, L. Carson, Titanium for orthopedic applications: an overview of surface modification to improve biocompatibility and prevent bacterial biofilm formation. Iscience 23(11), 101745 (2020)10.1016/j.isci.2020.101745 ↗
6. E.L. Cyphert, N.J. Zhang, G.D. Learn, C.J. Hernandez, H.A. von Recum, Recent advances in the evaluation of antimicrobial materials for resolution of orthopedic implant-associated infections in vivo. Acs Infect. Dis. 7(12), 3125–3160 (2021)10.1021/acsinfecdis.1c00465 ↗
7. K.G. Neoh, X.F. Hu, D. Zheng, E.T. Kang, Balancing osteoblast functions and bacterial adhesion on functionalized titanium surfaces. Biomaterials 33(10), 2813–2822 (2012)10.1016/j.biomaterials.2012.01.018 ↗
8. U. Filipovic, R.G. Dahmane, S. Ghannouchi, A. Zore, K. Bohinc, Bacterial adhesion on orthopedic implants. Adv. Colloid Interfac. 283, 102228 (2020)10.1016/j.cis.2020.102228 ↗
9. E.C. Rodriguez-Merchan, D.J. Davidson, A.D. Liddle, Recent strategies to combat infections from biofilm-forming bacteria on orthopaedic implants. Int. J. Mol. Sci. 22(19), 10243 (2021)10.3390/ijms221910243 ↗
10. G. Tan, J. Xu, W.M. Chirume, J.Y. Zhang, H. Zhang, X.F. Hu, Antibacterial and anti-inflammatory coating materials for orthopedic implants: a review. Coatings 11(11), 1401 (2021)10.3390/coatings11111401 ↗
11. R. Kargupta, S. Bok, C.M. Darr, B.D. Crist, K. Gangopadhyay, S. Gangopadhyay, S. Sengupta, Coatings and surface modifications imparting antimicrobial activity to orthopedic implants. WIRES Nanomed. Nanobi. 6(5), 475–495 (2014)10.1002/wnan.1273 ↗
12. J.H. Sui, S. Liu, M.C. Chen, H. Zhang, Surface bio-functionalization of anti-bacterial titanium implants: a review. Coatings 12(8), 1125 (2022)10.3390/coatings12081125 ↗
13. H. Chouirfa, H. Bouloussa, V. Migonney, C. Falentin-Daudre, Review of titanium surface modification techniques and coatings for antibacterial applications. Acta Biomater. 83, 37–54 (2019)10.1016/j.actbio.2018.10.036 ↗
14. Y.A. Qing, L. Cheng, R.Y. Li, G.C. Liu, Y.B. Zhang, X.F. Tang, J.C. Wang, H. Liu, Y.G. Qin, Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies. Int J Nanomed. 13, 3311–3327 (2018)10.2147/IJN.S165125 ↗
15. S. Chernousova, M. Epple, Silver as antibacterial agent: ion, nanoparticle, and metal. Angew. Chem. Int. Edit. 52(6), 1636–1653 (2013)10.1002/anie.201205923 ↗
16. U.V. Chetan, A systematic review of the interaction and effects generated by antimicrobial metallic substituents in bone tissue engineering. Metallomics 12(10), 1458–1479 (2020)10.1039/D0MT00127A ↗
17. M. Ramstedt, B. Ekstrand-Hammarstrom, A.V. Shchukarev, A. Bucht, L. Osterlund, M. Welch, W.T.S. Huck, Bacterial and mammalian cell response to poly(3-sulfopropyl methacrylate) brushes loaded with silver halide salts. Biomaterials 30(8), 1524–1531 (2009)10.1016/j.biomaterials.2008.12.008 ↗
18. X.J. He, X.Y. Zhang, L. Bai, R.Q. Hang, X.B. Huang, L. Qin, X.H. Yao, B. Tang, Antibacterial ability and osteogenic activity of porous Sr/Ag-containing TiO2 coatings. Biomed. Mater. 11(4), 045008 (2016)10.1088/1748-6041/11/4/045008 ↗
19. Y. Huang, Y.X. Zhang, M.Y. Li, H. Yang, J.Y. Liang, Y. Chen, Y.S. Zhang, X. Huang, L. Xie, H. Lin, H.X. Qiao, J.P. Lan, Physicochemical, osteogenic and antimicrobial properties of graphene oxide reinforced silver/strontium-doped hydroxyapatite on titanium for potential orthopedic applications. Surf. Coat. Tech. 446, 128788 (2022)10.1016/j.surfcoat.2022.128788 ↗
20. N.J. Lakhkar, I.H. Lee, H.W. Kim, V. Salih, I.B. Wall, J.C. Knowles, Bone formation controlled by biologically relevant inorganic ions: role and controlled delivery from phosphate-based glasses. Adv. Drug Deliver. Rev. 65(4), 405–420 (2013)10.1016/j.addr.2012.05.015 ↗
21. A. Hoppe, N.S. Guldal, A.R. Boccaccini, A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 32(11), 2757–2774 (2011)10.1016/j.biomaterials.2011.01.004 ↗
22. P. Habibovic, J.E. Barralet, Bioinorganics and biomaterials: bone repair. Acta Biomater. 7(8), 3013–3026 (2011)10.1016/j.actbio.2011.03.027 ↗
23. J.Q. You, Y.D. Zhang, Y.M. Zhou, Strontium functionalized in biomaterials for bone tissue engineering: a prominent role in osteoimmunomodulation. Front. Bioeng. Biotech. 10, 928799 (2022)10.3389/fbioe.2022.928799 ↗
24. W. Zhang, Y. Shen, H. Pan, K. Lin, X. Liu, B.W. Darvell, W.W. Lu, J. Chang, L. Deng, D. Wang, W. Huang, Effects of strontium in modified biomaterials. Acta Biomater. 7(2), 800–808 (2011)10.1016/j.actbio.2010.08.031 ↗
25. Z. Geng, R.F. Wang, X.L. Zhuo, Z.Y. Li, Y.C. Huang, L.L. Ma, Z.D. Cui, S.L. Zhu, Y.Q. Liang, Y.D. Liu, H.J. Bao, X. Li, Q.Y. Huo, Z.L. Liu, X.J. Yang, Incorporation of silver and strontium in hydroxyapatite coating on titanium surface for enhanced antibacterial and biological properties. Mater. Sci. Eng. C Mater. Bio. Appl. 71, 852–861 (2017)10.1016/j.msec.2016.10.079 ↗
26. Z. Geng, Z.D. Cui, Z.Y. Li, S.L. Zhu, Y.Q. Liang, Y.D. Liu, X. Li, X. He, X.X. Yu, R.F. Wang, X.J. Yang, Strontium incorporation to optimize the antibacterial and biological characteristics of silver-substituted hydroxyapatite coating. Mater. Sci. Eng. C Mater. Bio. Appl. 58, 467–477 (2016)10.1016/j.msec.2015.08.061 ↗
27. L. Besra, M. Liu, A review on fundamentals and applications of electrophoretic deposition (EPD). Prog. Mater Sci. 52(1), 1–61 (2007)10.1016/j.pmatsci.2006.07.001 ↗
28. K. Ma, X. Cai, Y. Zhou, Z. Zhang, T. Jiang, Y. Wang, Osteogenetic property of a biodegradable three-dimensional macroporous hydrogel coating on titanium implants fabricated via EPD. Biomed. Mater. 9(1), 015008 (2014)10.1088/1748-6041/9/1/015008 ↗
29. T. Jiang, Z. Zhang, Y. Zhou, Y. Liu, Z.W. Wang, H. Tong, X.Y. Shen, Y.N. Wang, Surface functionalization of titanium with chitosan/gelatin via electrophoretic deposition: characterization and cell behavior. Biomacromol 11(5), 1254–1260 (2010)10.1021/bm100050d ↗
30. K. Ma, D. Huang, J. Cai, X. Cai, L. Gong, P. Huang, Y. Wang, T. Jiang, Surface functionalization with strontium-containing nanocomposite coatings via EPD. Colloid. Surface. B 146, 97–106 (2016)10.1016/j.colsurfb.2016.05.036 ↗
31. K. Ma, L. Gong, X. Cai, P. Huang, J. Cai, D. Huang, T. Jiang, A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties. Int J Nanomed. 12, 3665–3679 (2017)10.2147/IJN.S130857 ↗
32. H. Cheng, W. Xiong, Z. Fang, H. Guan, W. Wu, Y. Li, Y. Zhang, M.M. Alvarez, B. Gao, K. Huo, J. Xu, N. Xu, C. Zhang, J. Fu, A. Khademhosseini, F. Li, Strontium (Sr) and silver (Ag) loaded nanotubular structures with combined osteoinductive and antimicrobial activities. Acta Biomater. 31, 388–400 (2016)10.1016/j.actbio.2015.11.046 ↗
33. Z. Wang, B. Li, Q. Cai, X. Li, Z. Yin, B. Li, Z. Li, W. Meng, Advances and prospects in antibacterial-osteogenic multifunctional dental implant surface. Front. Bioeng. Biotech. 10, 921338 (2022)10.3389/fbioe.2022.921338 ↗
34. S. Kligman, Z. Ren, C.H. Chung, M.A. Perillo, Y.C. Chang, H. Koo, Z. Zheng, C.S. Li, The impact of dental implant surface modifications on osseointegration and biofilm formation. J. Clin. Med. 10(8), 1641 (2021)10.3390/jcm10081641 ↗
35. E. Guibal, Interactions of metal ions with chitosan-based sorbents: a review. Sep. Purif. Technol. 38(1), 43–74 (2004)10.1016/j.seppur.2003.10.004 ↗
36. A. Radornska-Soukharev, Stability of lipid exciplents in solid lipid nanoparticles. Adv. Drug Deliver. Rev. 59(6), 411–418 (2007)10.1016/j.addr.2007.04.004 ↗
37. Y. Cheng, X. Luo, J. Betz, S. Buckhout-White, O. Bekdash, G.F. Payne, W.E. Bentley, G.W. Rubloff, In situ quantitative visualization and characterization of chitosan electrodeposition with paired sidewall electrodes. Soft Matter 6(14), 3177–3183 (2010)10.1039/c0sm00124d ↗
38. M. Mishra, S. Bhattacharjee, L. Besra, H.S. Sharma, T. Uchikoshi, Y. Sakka, Effect of pH localization on microstructure evolution of deposits during aqueous electrophoretic deposition (EPD). J. Eur. Ceram. Soc. 30(12), 2467–2473 (2010)10.1016/j.jeurceramsoc.2010.04.034 ↗
39. A.P. Abbott, K.J. McKenzie, Application of ionic liquids to the electrodeposition of metals. Phys. Chem. Chem. Phys. 8(37), 4265–4279 (2006)10.1039/b607329h ↗
40. V. Karageorgiou, D. Kaplan, Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26(27), 5474–5491 (2005)10.1016/j.biomaterials.2005.02.002 ↗
41. P. Huang, K. Ma, X. Cai, D. Huang, X. Yang, J. Ran, F. Wang, T. Jiang, Enhanced antibacterial activity and biocompatibility of zinc-incorporated organic-inorganic nanocomposite coatings via electrophoretic deposition. Colloid. Surface. B 160, 628–638 (2017)10.1016/j.colsurfb.2017.10.012 ↗
42. S.Z. Li, H. Zhang, J. Xu, D. Yang, Hydrothermal synthesis of flower-like SrCO3 nanostructures. Mater. Lett. 59(4), 420–422 (2005)10.1016/j.matlet.2004.09.037 ↗
43. W. Hu, S. Chen, X. Li, S. Shi, W. Shen, X. Zhang, H. Wang, In situ synthesis of silver chloride nanoparticles into bacterial cellulose membranes. Mater. Sci. Eng. C Mater. Bio. Appl. 29(4), 1216–1219 (2009)10.1016/j.msec.2008.09.017 ↗
44. Y. Herdiana, N. Wathoni, S. Shamsuddin, M. Muchtaridi, Drug release study of the chitosan-based nanoparticles. Heliyon 8(1), e08674 (2022)10.1016/j.heliyon.2021.e08674 ↗
45. M. Goldberg, R. Langer, X.Q. Jia, Nanostructured materials for applications in drug delivery and tissue engineering. J. Biomat. Sci.-Polym. E. 18(3), 241–268 (2007)10.1163/156856207779996931 ↗
46. R.A. Petros, J.M. DeSimone, Strategies in the design of nanoparticles for therapeutic applications. Nat. Rev. Drug Discov. 9(8), 615–627 (2010)10.1038/nrd2591 ↗
47. Y.J. Kuo, C.H. Chen, P. Dash, Y.C. Lin, C.W. Hsu, S.J. Shih, R.J. Chung, Angiogenesis, osseointegration, and antibacterial applications of polyelectrolyte multilayer coatings incorporated with silver/strontium containing mesoporous bioactive glass on 316L stainless steel. Front. Bioeng. Biotech. 10, 818137 (2022)10.3389/fbioe.2022.818137 ↗
48. R.O. Darouiche, Current concepts—treatment of infections associated with surgical implants. N. Engl. J. Med. 350(14), 1422–1429 (2004)10.1056/NEJMra035415 ↗
49. V. Sambhy, M.M. MacBride, B.R. Peterson, A. Sen, Silver bromide nanoparticle/polymer composites: dual action tunable antimicrobial materials. J. Am. Chem. Soc. 128(30), 9798–9808 (2006)10.1021/ja061442z ↗
50. M. Rai, A. Yadav, A. Gade, Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv. 27(1), 76–83 (2009)10.1016/j.biotechadv.2008.09.002 ↗
51. A.S. Kazachenko, E.V. Legler, O.V. Per’yanova, Y.A. Vstavskaya, Synthesis and antimicrobial activity of silver complexes with histidine and tryptophan. Khim.-Farm. Zh. 34(5), 34–35 (2000)
52. O. Choi, K.K. Deng, N.-J. Kim, L. Ross Jr., R.Y. Surampalli, Z. Hu, The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth. Water Res. 42(12), 3066–3074 (2008)10.1016/j.watres.2008.02.021 ↗
53. J. Braux, F. Velard, C. Guillaume, S. Bouthors, E. Jallot, J.M. Nedelec, D. Laurent-Maquin, P. Laquerriere, A new insight into the dissociating effect of strontium on bone resorption and formation. Acta Biomater. 7(6), 2593–2603 (2011)10.1016/j.actbio.2011.02.013 ↗
54. L. Zhao, H. Wang, K. Huo, X. Zhang, W. Wang, Y. Zhang, Z. Wu, P.K. Chu, The osteogenic activity of strontium loaded titania nanotube arrays on titanium substrates. Biomaterials 34(1), 19–29 (2013)10.1016/j.biomaterials.2012.09.041 ↗