Preparation and characterization of Bomidin-loaded thermosensitive hydrogel for periodontal application
Source abstract
Abstract
AbstractPeriodontitis is a chronic infection and tissue-destructive disease which is considered as the major cause of tooth loss. However, satisfactory clinical treatment for the disease has yet to be achieved. Herein, we designed an injectable thermo-sensitive hydrogel consisting of hydroxypropyl methylcellulose (HPMC), hyaluronic acid (HA) and glycerol (Gl), and further loaded with Bomidin (FP), a recombinant antimicrobial peptide (AMP), the physicochemical and biological characteristics were subsequently investigated in vitro. The results indicated that the prepared hydrogel can be injected and changes phase in situ at desirable temperature. Also, a three-dimensional network structure and cell compatibility were observed. Importantly, the FP-embedded hydrogels exhibited strong antibacterial activities against oral pathogensPorphyromonas gingivalis(P. gingivalis),Staphylococcus aureus(S. aureus), andEscherichia coli(E. coli). These findings indicated our hydrogel possesses not only good fluidity, stable thermosensitivity but also preferable biocompatibility and antibacterial properties, thus demonstrate promising potential for application in periodontal treatment.Trial registrationPJ2020-113-001, registration date: Jun 2019.Graphical abstract
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Oral microbiology and periodontitis research · Antimicrobial Peptides and Activities · Salivary Gland Disorders and Functions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (48)
Show source-provided references
1. X. Chen, G. Wu, Z. Feng, Y. Dong, W. Zhou, B. Li, S. Bai, Y. Zhao, Advanced biomaterials and their potential applications in the treatment of periodontal disease. Crit. Rev. Biotechnol. 36, 760–775 (2016)10.3109/07388551.2015.1035693 ↗
2. D.F. Kinane, P.G. Stathopoulou, P.N. Papapanou, Periodontal diseases. Nat. Rev. Dis. Primers 3, 17038 (2017)10.1038/nrdp.2017.38 ↗
3. J. Slots, Periodontitis: facts, fallacies and the future. Periodontol 2000(75), 7–23 (2017)10.1111/prd.12221 ↗
4. M. Feres, L.C. Figueiredo, G.M.S. Soares, M. Faveri, Systemic antibiotics in the treatment of periodontitis. Periodontol 2000(67), 131–186 (2015)10.1111/prd.12075 ↗
5. F. Koch, K. Ekat, D. Kilian, T. Hettich, O. Germershaus, H. Lang, K. Peters, B. Kreikemeyer, A versatile biocompatible antibiotic delivery system based on self-assembling peptides with antimicrobial and regenerative potential. Adv. Healthc. Mater. 8, e1900167 (2019)10.1002/adhm.201900167 ↗
6. C. Manresa, E.C. Sanz-Miralles, J. Twigg, M. Bravo, Supportive periodontal therapy (SPT) for maintaining the dentition in adults treated for periodontitis. Cochrane Database Syst. Rev. 1, CD009376 (2018)
7. S. Sharma, S. Tiwari, A review on biomacromolecular hydrogel classification and its applications. Int. J. Biol. Macromol. 162, 737–747 (2020)10.1016/j.ijbiomac.2020.06.110 ↗
8. R. Dimatteo, N.J. Darling, T. Segura, In situ forming injectable hydrogels for drug delivery and wound repair. Adv. Drug Deliv. Rev. 127, 167–184 (2018)10.1016/j.addr.2018.03.007 ↗
9. G. Milcovich, S. Lettieri, F.E. Antunes, B. Medronho, A.C. Fonseca, J.F.J. Coelho, P. Marizza, F. Perrone, R. Farra, B. Dapas, G. Grassi, M. Grassi, S. Giordani, Recent advances in smart biotechnology: Hydrogels and nanocarriers for tailored bioactive molecules depot. Adv. Colloid. Interface Sci. 249, 163–180 (2017)10.1016/j.cis.2017.05.009 ↗
10. S. Liang, Y. Zhang, H. Wang, Z. Xu, J. Chen, R. Bao, B. Tan, Y. Cui, G. Fan, W. Wang, W. Wang, W. Liu, Paintable and rapidly bondable conductive hydrogels as therapeutic cardiac patches. Adv. Mater. 30, e1704235 (2018)10.1002/adma.201704235 ↗
11. X. Wang, Z. Li, T. Shi, P. Zhao, K. An, C. Lin, H. Liu, Injectable dextran hydrogels fabricated by metal-free click chemistry for cartilage tissue engineering. Mater. Sci. Eng. C Mater. Biol. Appl. 73, 21–30 (2017)10.1016/j.msec.2016.12.053 ↗
12. C.A.R. Barragán, E.R.M. Balleza, L. García-Uriostegui, J.A.A. Ortega, G. Toríz, E. Delgado, Rheological characterization of new thermosensitive hydrogels formed by chitosan, glycerophosphate, and phosphorylated β-cyclodextrin. Carbohydr. Polym. 201, 471–481 (2018)10.1016/j.carbpol.2018.08.076 ↗
13. Z. Zheng, S. Bian, Z. Li, Z. Zhang, Y. Liu, X. Zhai, H. Pan, X. Zhao, Catechol modified quaternized chitosan enhanced wet adhesive and antibacterial properties of injectable thermo-sensitive hydrogel for wound healing. Carbohydr. Polym. 249, 116826 (2020)10.1016/j.carbpol.2020.116826 ↗
14. J. Zhu, X. Tang, Y. Jia, C.T. Ho, Q. Huang, Applications and delivery mechanisms of hyaluronic acid used for topical/transdermal delivery - A review. Int. J. Pharm. 578, 119127 (2020)10.1016/j.ijpharm.2020.119127 ↗
15. W. Zhang, X. Jin, H. Li, R.R. Zhang, C.W. Wu, Injectable and body temperature sensitive hydrogels based on chitosan and hyaluronic acid for pH sensitive drug release. Carbohydr. Polym. 186, 82–90 (2018)10.1016/j.carbpol.2018.01.008 ↗
16. M. Eliezer, J.C. Imber, A. Sculean, N. Pandis, S. Teich, Hyaluronic acid as adjunctive to non-surgical and surgical periodontal therapy: a systematic review and meta-analysis. Clin. Oral Investig. 23, 3423–3435 (2019)10.1007/s00784-019-03012-w ↗
17. Y. Xue, H. Chen, C. Xu, D. Yu, H. Xua, Y. Hu, Synthesis of hyaluronic acid hydrogels by crosslinking the mixture of high-molecular-weight hyaluronic acid and low-molecular-weight hyaluronic acid with 1,4-butanediol diglycidyl ether. RSC Adv. 10, 7206–7213 (2020)10.1039/C9RA09271D ↗
18. M. Yang, S.Y. Lee, S. Kim, J.S. Koo, J.H. Seo, D.I. Jeong, C. Hwang, J. Lee, H.J. Cho, Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy. J. Control Release 324, 750–764 (2020)10.1016/j.jconrel.2020.04.024 ↗
19. K. Dharmalingam, R. Anandalakshmi, Fabrication, characterization and drug loading efficiency of citric acid crosslinked NaCMC-HPMC hydrogel films for wound healing drug delivery applications. Int. J. Biol. Macromol. 134, 815–829 (2019)10.1016/j.ijbiomac.2019.05.027 ↗
20. H. Khanum, K. Ullah, G. Murtaza, S.A. Khan, Fabrication and in vitro characterization of HPMC-g-poly(AMPS) hydrogels loaded with loxoprofen sodium. Int. J. Biol. Macromol. 120, 1624–1631 (2018)10.1016/j.ijbiomac.2018.09.184 ↗
21. S. Perez-Robles, C. Carotenuto, M. Minale, HPMC hydrogel formation mechanisms unveiled by the evaluation of the activation energy. Polymers 14, 635 (2022)10.3390/polym14030635 ↗
22. S.M.C. Silva, F.V. Pinto, F.E. Antunes, M.G. Miguel, J.J.S. Sousa, A.C.C. Pais, Aggregation and gelation in hydroxypropylmethyl cellulose aqueous solutions. J. Colloid. Interface Sci. 327, 333–340 (2008)10.1016/j.jcis.2008.08.056 ↗
23. N. Almeida, L. Rakesh, J. Zhao, Phase behavior of concentrated hydroxypropyl methylcellulose solution in the presence of mono and divalent salt. Carbohydr. Polym. 99(99), 630–637 (2014)10.1016/j.carbpol.2013.08.081 ↗
24. J.C. Richardson, C.S. Foster, S.W. Doughty, J.S. Burton, R.J. MacRae, C.D. Melia, The influence of l-amino acid molecular structure on the phase transition temperature of hydroxypropyl methylcellulose. Carbohydr. Polym. 65, 22–27 (2006)10.1016/j.carbpol.2005.11.022 ↗
25. T. Wang, L. Chen, T. Shen, D. Wu, Preparation and properties of a novel thermo-sensitive hydrogel based on chitosan/hydroxypropyl methylcellulose/glycerol. Int. J. Biol. Macromol. 93, 775–782 (2016)10.1016/j.ijbiomac.2016.09.038 ↗
26. Y. Chen, Y. Zhang, X. Wang, J. Ling, G. He, L. Shen, Antibacterial activity and its mechanisms of a recombinant Funme peptide against Cronobacter sakazakii in powdered infant formula. Food Res. Int. 116, 258–265 (2019)10.1016/j.foodres.2018.08.030 ↗
27. X. Yang, Y. Hou, T. Gong, L. Sun, J. Xue, Y. Guo, Concentration-dependent rheological behavior and gelation mechanism of high acyl gellan aqueous solutions. Int. J. Biol. Macromol. 131, 959–970 (2019)10.1016/j.ijbiomac.2019.03.137 ↗
28. Y. Wang, Y. Long, F. Xie, L. Sheng, Q. Sun, H. Liu, C. Ling, On the investigation of thermal/cooling-gel biphasic systems based on hydroxypropyl methylcellulose and hydroxypropyl starch. Ind. Crops Prod. 132, 413–429 (2019)10.1016/j.indcrop.2019.02.051 ↗
29. J.D.D.S. Carvalho, R.S. Rabelo, M.D. Hubinger, Thermo-rheological properties of chitosan hydrogels with hydroxypropyl methylcellulose and methylcellulose. Int. J. Biol. Macromol. 209, 367–375 (2022)10.1016/j.ijbiomac.2022.04.035 ↗
30. H.F. Darge, A.T. Andrgie, H.C. Tsai, J.Y. Lai, Polysaccharide and polypeptide based injectable thermo-sensitive hydrogels for local biomedical applications. Int. J. Biol. Macromol. 133, 545–563 (2019)10.1016/j.ijbiomac.2019.04.131 ↗
31. Y.C. Chen, Y.H. Chen, Thermo and pH-responsive methylcellulose and hydroxypropyl methylcellulose hydrogels containing K2SO4 for water retention and a controlled-release water-soluble fertilizer. Sci. Total Environ. 655, 958–967 (2019)10.1016/j.scitotenv.2018.11.264 ↗
32. S. Allahbash, T. Nicolai, C. Chassenieux, J.F. Tassin, L. Benyahia, P. Weiss, G. Rethore, Interplay of thermal and covalent gelation of silanized hydroxypropyl methyl cellulose gels. Carbohydr. Polym 115, 510–515 (2015)10.1016/j.carbpol.2014.08.067 ↗
33. D. Gupta, C.H. Tator, M.S. Shoichet, Fast-gelling injectable blend of hyaluronan and methylcellulose for intrathecal, localized delivery to the injured spinal cord. Biomaterials 27, 2370–2379 (2006)10.1016/j.biomaterials.2005.11.015 ↗
34. E. Kutálková, J. Hrnčiřík, R. Witasek, M. Ingr, Effect of solvent and ions on the structure and dynamics of a hyaluronan molecule. Carbohydr. Polym. 234, 115919 (2020)10.1016/j.carbpol.2020.115919 ↗
35. Q. Kuang, G. Cheng, J. Zhao, Y. Li, Thermogelation hydrogels of methylcellulose and glycerol-methylcellulose systems. J. Appl. Polym. Sci. 100, 4120–4126 (2010)10.1002/app.23375 ↗
36. P. Snetkov, K. Zakharova, S. Morozkina, R. Olekhnovich, M. Uspenskaya, Hyaluronic acid: the influence of molecular weight on structural, physical, physico-chemical, and degradable properties of biopolymer. Polymers 12, 1800 (2020)10.3390/polym12081800 ↗
37. A. Fallacara, E. Baldini, S. Manfredini, S. Vertuani, Hyaluronic acid in the third millennium. Polymers 10, 701 (2018)10.3390/polym10070701 ↗
38. H. Liu, J. Liu, C. Qi, Y. Fang, L. Zhang, R. Zhuo, X. Jiang, Thermosensitive injectable in-situ forming carboxymethyl chitin hydrogel for three-dimensional cell culture. Acta Biomater. 35, 228–237 (2016)10.1016/j.actbio.2016.02.028 ↗
39. Z. Li, H. Shim, M.O. Cho, I.S. Cho, J.H. Lee, S.W. Kang, B. Kwon, K.M. Huh, Thermo-sensitive injectable glycol chitosan-based hydrogel for treatment of degenerative disc disease. Carbohydr. Polym. 184, 342–353 (2018)10.1016/j.carbpol.2018.01.006 ↗
40. Q. Wang, Q. Wang, W. Teng, Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications. Int. J. Nanomedicine 11, 131–144 (2016)10.2147/IJN.S94777 ↗
41. J.A. Burdick, G.D. Prestwich, Hyaluronic acid hydrogels for biomedical applications. Adv. Mater. 12, H41–H56 (2011)10.1002/adma.201003963 ↗
42. M.F.P. Graça, S.P. Miguel, C.S.D. Cabral, I.J. Correia, Hyaluronic acid-Based wound dressings: A review. Carbohydr. Polym. 241, 116364 (2020)10.1016/j.carbpol.2020.116364 ↗
43. Z. Zhang, D. Liu, S. Liu, S. Zhang, Y. Pan, The role of outer membrane vesicles in periodontal disease and related systemic diseases. Front. Cell Infect. Microbiol. 10, 585917 (2021)10.3389/fcimb.2020.585917 ↗
44. M. Zhang, Y. Yu, L. Lian, W. Li, J. Ren, Y. Liang, F. Xue, F. Tang, X. Zhu, J. Ling, J. Dai, Functional Mechanism of Antimicrobial Peptide Bomidin and Its Safety for Macrobrachium rosenbergii. Probiotics Antimicrob Proteins 14, 169–179 (2022)10.1007/s12602-021-09857-6 ↗
45. B. Gottenbos, D.W. Grijpma, H.C. van der Mei, J. Feijen, H.J. Busscher, Antimicrobial effects of positively charged surfaces on adhering Gram-positive and Gram-negative bacteria. J. Antimicrob. Chemother. 48, 7–13 (2001)10.1093/jac/48.1.7 ↗
46. R. Liu, Z. Liu, H. Peng, Y. Lv, Y. Feng, J. Kang, N. Lu, R. Ma, S. Hou, W. Sun, Q. Ying, F. Wang, Q. Gao, P. Zhao, C. Zhu, Y. Wang, X. Wu, Bomidin: An optimized antimicrobial peptide with broad antiviral activity against enveloped viruses. Front. Immunol. 13, 851642 (2022)10.3389/fimmu.2022.851642 ↗
47. L. Peng, L. Chang, M. Si, J. Lin, Y. Wei, S. Wang, H. Liu, B. Han, L. Jiang, Hydrogel-coated dental device with adhesion-inhibiting and colony-suppressing properties. ACS Appl. Mater. Interfaces 12, 9718–9725 (2020)10.1021/acsami.9b19873 ↗
48. X. Xu, Y. Shen, W. Wang, C. Sun, C. Li, Y. Xiong, J. Tu, Preparation and in vitrocharacterization of thermosensitive and mucoadhesive hydrogels for nasal delivery of phenylephrine hydrochloride. Eur. J. Pharm. Biopharm. 88, 998–1004 (2014)10.1016/j.ejpb.2014.08.015 ↗