Development of cholesterol imprinted polymer-based interfaces as smart sensors for detection of cholesterol in clinical samples
Source abstract
Abstract
Molecularly imprinted polymers (MIP) based on biomimetic approaches have proven their capabilities for creating highly specific receptor materials. We developed and investigated a biosensor based on MIP for the selective detection of cholesterol in synthetic media as well as in human blood sera. The surface roughness of imprinted interface increased to 4 nm as compared to non-imprinted indicating the presence of cholesterol-specific cavities. The concentration of cholesterol was related to the flow of charge across the interface of microchip. The biosensor showed a good linear relationship with the concentration of cholesterol ranging from 0.01 to 1 mM with a low limit of detection as 0.31 mM. The interface specifically recognized cholesterol three times as compared to cholecalciferol. The average clinical recovery rate of 98.81% indicates promising potential for an alternate to enzymatic biosensors for the determination of cholesterol. Graphical abstract: (Figure presented.)
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Analytical chemistry methods development · Pharmacological Effects and Assays · Electrochemical sensors and biosensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (34)
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1. O. López-Fernández et al., Comparison between HPLC-PAD and GC-MS methods for the quantification of cholesterol in meat. Food Anal. Methods 15(4), 1118–1131 (2022)10.1007/s12161-021-02226-7 ↗
2. K. Derina et al., Non-enzymatic electrochemical approaches to cholesterol determination. J. Pharm. Biomed. Anal. 191, 113538 (2020)10.1016/j.jpba.2020.113538 ↗
3. H.M. Yadav et al., Recent development in nanomaterial-based electrochemical sensors for cholesterol detection. Chemosensors 9(5), 98 (2021)10.3390/chemosensors9050098 ↗
4. L. Liu et al., Colorimetric detection of cholesterol based on the peroxidase-like activity of metal-organic framework MIL-101(Cr). ChemistrySelect 6(28), 7143–7149 (2021)10.1002/slct.202102053 ↗
5. Y. Ning et al., Glow-type chemiluminescent hydrogels for point-of-care testing (POCT) of cholesterol. Analyst 146(15), 4775–4780 (2021)10.1039/D1AN00676B ↗
6. T.T.N. Anh et al., Cerium oxide/Polypyrrole nanocomposite as the matrix for cholesterol biosensor. Adv. Polym. Technol. 2021, 6627645 (2021)10.1155/2021/6627645 ↗
7. A.A. Ansari, B.D. Malhotra, Current progress in organic–inorganic hetero-nano-interfaces based electrochemical biosensors for healthcare monitoring. Coord. Chem. Rev. 452, 214282 (2022)10.1016/j.ccr.2021.214282 ↗
8. V.S. Haritha et al., Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode. Mater. Today: Proceed. 50, 34–39 (2022)
9. U. Latif et al., Nanostructured molecularly imprinted photonic polymers for sensing applications. Curr. Nanosci. 16(4), 495–503 (2020)10.2174/1573413715666190206144415 ↗
10. H. Shokrani et al., Polysaccharide-based nanocomposites for biomedical applications: a critical review. Nanoscale Horizons 7(10), 1136–1160 (2022)10.1039/D2NH00214K ↗
11. A. Garcia Cruz et al., Design and fabrication of a smart sensor using in silico epitope mapping and electro-responsive imprinted polymer nanoparticles for determination of insulin levels in human plasma. Biosens. Bioelectron. 169, 112536 (2020)10.1016/j.bios.2020.112536 ↗
12. S.A.I. Jariya et al., Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis. Mater. Chem. Phys. 272, 125019 (2021)10.1016/j.matchemphys.2021.125019 ↗
13. A. Nasrullah et al., ZnO nanoparticles and β-cyclodextrin containing molecularly imprinted polymers for gravimetric sensing of very-low-density lipoprotein. Measure. Sci. Technol. 33(4), 045106 (2022)10.1088/1361-6501/ac471c ↗
14. A.-M. Gavrilă et al., Modern and Dedicated methods for producing molecularly imprinted polymer layers in sensing applications. Appl. Sci. 12(6), 3080 (2022)10.3390/app12063080 ↗
15. W.M. Altogbia et al., Molecular imprinted polymer for β-carotene for application in palm oil mill effluent treatment. Arab. J. Chem. 14(2), 102928 (2021)10.1016/j.arabjc.2020.102928 ↗
16. G. Alberti et al., Current trends in polymer based sensors. Chemosensors 9(5), 108 (2021)10.3390/chemosensors9050108 ↗
17. S. Khan et al., Preparation of oxidized Zn–In nanostructures for electrochemical non-enzymatic cholesterol sensing. Mater. Sci. Semicond. Process. 135, 106101 (2021)10.1016/j.mssp.2021.106101 ↗
18. F. Kartal, A., Denizli: molecularly imprinted cryogel beads for cholesterol removal from milk samples. Colloids Surf. B Biointerfaces 190, 110860 (2020)10.1016/j.colsurfb.2020.110860 ↗
19. A.B. Hashkavayi et al., Development of core-shell magnetic molecularly imprinted polymer-based electrochemical sensor for sensitive and selective detection of ezetimibe. Heliyon 9, e17169 (2023)10.1016/j.heliyon.2023.e17169 ↗
20. B. Lakard, Electrochemical biosensors based on conducting polymers: a review. Appl. Sci. 10(18), 6614 (2020)10.3390/app10186614 ↗
21. S. El-Akaad et al., Capacitive sensor based on molecularly imprinted polymers for detection of the insecticide imidacloprid in water. Sci. Rep. 10(1), 14479 (2020)10.1038/s41598-020-71325-y ↗
22. M. Amiri, S. Arshi, An overview on electrochemical determination of cholesterol. Electroanalysis 32(7), 1391–1407 (2020)10.1002/elan.201900669 ↗
23. M. Nemiwal et al., Enzyme immobilized nanomaterials as electrochemical biosensors for detection of biomolecules. Enzyme and Microb. Technol. 156, 110006 (2022)10.1016/j.enzmictec.2022.110006 ↗
24. N. Sabrina et al., Molecularly Imprinted Polymer (MIPs) nanomaterials modified as absorbing cholesterol. J. Phys.: Conf. Series 1788(1), 012001 (2021)
25. J. Niesa, A. Ulianas, Design and characterization of membrane molecularly imprinted polymer (MIP) as cholesterol absorbent. J. Phys.: Conf. Series 1481(1), 012031 (2020)
26. T.S. Anirudhan et al., Electrochemical sensing of cholesterol by molecularly imprinted polymer of silylated graphene oxide and chemically modified nanocellulose polymer. Mater. Sci. Eng. C 92, 942–956 (2018)10.1016/j.msec.2018.07.041 ↗
27. A. Munawar et al., Investigating nanohybrid material based on 3D CNTs@Cu nanoparticle composite and imprinted polymer for highly selective detection of chloramphenicol. J. Hazard. Mater. 342, 96–106 (2018)10.1016/j.jhazmat.2017.08.014 ↗
28. A.I. Gopalan et al., Development of a stable cholesterol biosensor based on multi-walled carbon nanotubes–gold nanoparticles composite covered with a layer of chitosan–room-temperature ionic liquid network. Biosens. Bioelectron. 24(7), 2211–2217 (2009)10.1016/j.bios.2008.11.034 ↗
29. F.N. Maluin et al., Synthesis of PANI/hematite/PB hybrid nanocomposites and fabrication as screen printed paper based sensors for cholesterol detection. Anal. Methods 8(45), 8049–8058 (2016)10.1039/C6AY02478E ↗
30. V. Gautam et al., Polyaniline/MWCNTs/starch modified carbon paste electrode for non-enzymatic detection of cholesterol: application to real sample (cow milk). Anal. Bioanaly. Chem. 410(8), 2173–2181 (2018)10.1007/s00216-018-0880-6 ↗
31. V. Román-Pizarro et al., Usefulness of magnetically-controlled MNPs-enzymes microreactors for the fluorimetric determination of total cholesterol in serum. Talanta 208, 120426 (2020)10.1016/j.talanta.2019.120426 ↗
32. A. Rafiq et al., Biologically prepared copper-graphene nanohybrid as the interface of microchips for sensitive detection of crop viruses. J. Market. Res. 12, 727–738 (2021)
33. K. Bano et al., Development of biocompatible 1D CuO nanoneedles and their potential for sensitive, mass-based detection of anti-tuberculosis drugs. Appl. Nanosci. 9(6), 1341–1351 (2019)10.1007/s13204-019-01003-7 ↗
34. S.Z. Bajwa et al., Molecularly imprinted polymers for conductance sensing of Cu2+ in aqueous solutions. Sens. Actuators B: Chem. 192, 522–528 (2014)10.1016/j.snb.2013.11.013 ↗