Immobilization of glucose oxidase and catalase on magnetite nanoparticles as functional catalyst supports
Source abstract
Abstract
In this work, we have prepared magnetite nanoparticles (MNPs) coated with polyethylenimine (PEI) to obtain a positively charged surface and showed their suitability as functional supports to successfully immobilize glucose oxidase (GOx) and catalase (CAT) enzymes. We have compared two immobilization strategies, namely electrostatic binding and covalent attachment mediated by glutaraldehyde cross-linking. To quantify the amount of immobilized enzyme (q max), we have developed a methodological approach that minimizes the dependence of the q max value on the equilibrium constant of the adsorption process (K). Catalytic studies showed high retention of enzymatic activity (100% for covalent binding and 75% for electrostatic binding), indicating that the immobilization protocol preserves the native conformation of the enzyme. Furthermore, the covalent strategy demonstrated stable binding even when the nanohybrid was subjected to extreme conditions (pH 3), retaining 91% of the enzyme on the surface, compared to 6% when immobilized electrostatically. These results highlight the importance of surface engineering in the design of magnetic biocatalysts for potential application in starvation or oxygen generation therapies.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Electrochemical sensors and biosensors · Enzyme Catalysis and Immobilization · Advanced Nanomaterials in Catalysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Instituto de Nanociencia y Materiales de Aragon (INMA) CSIC-Universidad de Zaragoza, Campus Rio Ebro, Edificio I + D, C/Poeta Mariano Esquillor, s/n, 50018, Zaragoza, Spain
Department of Chemical and Environmental Engineering, University of Zaragoza, Campus Rio Ebro, C/María de Luna, 3, 50018 Zaragoza, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain
Instituto de Investigación Sanitaria (IIS) de Aragón, Avenida San Juan Bosco, 13, 50009 Zaragoza, Spain
Instituto de Investigación en Biotecnología y Salud (IDiBE), Universidad Miguel Hernández, 03202 Elche, Spain
Escuela Politécnica Superior, U. Zaragoza, Crta. de Cuarte s/n, 22071, Huesca, Spain
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias de la Salud y del Deporte, Campus de Huesca, U. Zaragoza, Spain
Funding
Agencia Estatal de Investigación · Generalitat Valenciana · European Research Council
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