Bio-inspired terpolymers containing dopamine, cations and MPC: a versatile platform to construct a recycle antibacterial and antifouling surface

A new kind of bio-inspired terpolymer was synthesized by a conventional free radical terpolymerization of dopamine methacrylamide (DMA), 2-(dimethylamino)-ethyl methacrylate (DMAEMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) with azobisisobutyronitrile (AIBN) as an initiator. DMA consists of a biomimetic adhesive side chain covalently linked to a polymerizable methacrylate monomer. ¹H NMR and gel permeation chromatography confirmed the successful synthesis of P(DMA-co-MPC-co-DMAEMA). The terpolymer could self-assemble on the macroscopic planar substrates with DMA as an anchor. After being quaternized by 1-bromo-heptane, terpolymers of P(DMA-co-MPC-co-DMAEMA⁺) with bactericidal function were obtained. The self-assembly terpolymer on the substrate was confirmed by X-ray photoelectron spectroscopy, water contact angle, spectroscopic ellipsometry and atomic force microscopy. The hydrophilicity and antifouling properties of the self-assembly coating increased greatly against bacteria, protein and cells with the increase of MPC content. As the existence of bactericidal cations for electrostatic targeting of bacteria as well as membrane lysis, the terpolymer coating showed excellent bactericidal function against E. coli and S. aureus. Biofilm inhibition assay showed that terpolymer coating was very efficient to resist bacterial adhesion and biofilm formation in a nutrient environment. Bacteria could be continuously "captured" and killed by the terpolymer coating, and then bacteria corpse was released into the solution. Importantly, this work provides a versatile strategy for the fabrication of a recycle antibacterial and antifouling surface to modify biomaterials.