The Study on inhibition and regulation of spruce mannan on enzymatic hydrolysis of cellulose

Mannan is the main hemicellulose in coniferous wood. Spruce mannan was extracted and characterized by FT-IR, SEM and specific surface area test. FT-IR showed it was branched galactoglucomannan; SEM revealed its layered surface and rod-like polymerization units with spherical tops at high magnification; it had small specific surface area and large pore size. Hydrolysis experiments showed spruce mannan inhibited commercial second-generation cellulase (max inhibition rate 22.22%), with inhibition related to hydrolysis time, enzyme dosage and mannan amount. Tween 80 alleviated this inhibition (min inhibition rate 8.69%), promoted β-glucosidase at high concentration (max promotion rate 28.57%), and greatly reduced mannan’s hydrolysis effect (inhibition rate down to 31.82%). This study provides a basis for improving mannan utilization and biomass energy conversion.