Fe Element Analysis in the Spleen Tissue by Using EDS and ICP-MS after Aminated Silica Shelled Magnetite (Fe₃O₄) Administered to the Tail Vein of Mice

The suppression of cytokine storm in severe coronavirus disease 2019 (COVID-19) patients can be treated with monoclonal antibody therapy against CD3 for T cell receptor inhibition. An optimized liquid phase as a CD3 antibody-magnetic nanoparticle (Ab-MNP) conjugate can inhibit the overactivation of T cells. We aim to analyze the distribution of Fe in the spleen after acute administration of silica-conjugated amine magnetite (Fe₃O₄) nanoparticles (35 nm) delivered by intravenous injection. The Fe element distribution and concentration levels in spleen tissue were analyzed using energy dispersive spectroscopy (EDS) and inductively coupled plasmamass spectrometry (ICP-MS). The experimental result is a difference in the concentration of Fe elements, which was 1.89×10³ mg/kg in the spleen of a control mouse not administered with MNPs, whereas increases significantly to 1.93×10³ mg/kg in that of a mouse administered with MNPs. Further, time kinetic analysis of biochemical and immunological parameters is required to confirm its suitability in bio-administration.