Use of dispersive Raman spectroscopy to detect the cytotoxic action of viscum album in adenocarcinoma of colon

In the past decades it was noticed that there was a gradual increase in neoplasia diagnostics in animals, which are attributed to the longevity achieved by efficient vaccines, antibiotics, diagnostics, and food quality. The aliphatic oncology uses chemotherapics that are citostatic association, citostatic antibiotics, antimetabolic citostatics, and antimetabolic hormones in tumor treatment. Later on, the oncology introduced the imunomodulated antineoplasics from vegetals which belongs the viscum album. This study verified the efficiency of the extract of the injectable viscum album with concentrations D3 (0.1 μg/mL), D6 (0.1 ng/mL), D9 (0.1×10−3 ng/mL), D12 (0.1×10−6 ng/mL), and D30 (0.1×10−24 ng/mL) in culture of Caco-2 cells in vitro evaluated by correlating the spectral information obtained by dispersive Raman spectroscopy at 830 nm excitation with the cell viability determined by the Mitochondrial Activity (MTT) assay test. Results of MTT indicated that viscum album acted in the mitochondrial activity by reducing cellular viability in the Caco-2 cells in the concentrations of D3, D6, and D9. It was found that there was a good correlation of the Raman spectrum analyzed by principal components analysis, with the values of the MTT, and such result indicated that Raman could constitute in a sensitive tool for the detection of changes in the mitochondrial activity of Caco-2 cells.