Surface Modification of Apatite-wollastonite Bioactive Glass-ceramic by Grafting with RGD Peptides

Tripeptide of arginine-glycine-aspartic acid (RGD) has been introduced to modify of biomaterials due to its high bio-activity, which could increase the cell adhesion. In this study, the apatite-wollastonite bioactive glass-ceramic (AW) with RGD peptide (RGD-AW) was fabricated. Firstly, the amino groups were introduced to the AW surface via low temperature plasma treatment. Then, RGD peptide were immobilized on the surface of AW by combination with the amino groups. The XRD, XPS and ATR-FTIR results confirmed that the RGD peptide were fixed on the surface of AW successfully. In order to testify whether the RGD-AW were more favorable to the cells, MG63 osteblast-like cells were employed to co-culture with RGD-AW for 4 h, 1 d and 5 d in vitro. The results of fluorescence microscopy, SEM and MTT assay showed that RGD-AW could enhance the attachment and proliferation of MG63 cells significantly compared with AW.