DataVXSmall StructuresPaper
journal article·2024-12Open access

Super‐Resolution Imaging in Collagen‐Abundant Thick Tissues

Ya‐Han Chuang, Yueh‐Feng Wu, Ya‐Hui Lin, Yin‐Hsu Chen, Yu‐Xian Zhou, Shao‐Chun Hsu, Hsin‐Mei Lee, Ann‐Shyn Chiang, Yunching Chen, Shiang‐Jiuun Chen, Sung‐Jan Lin, Li‐An Chu

Small Structures· Volume 5· Issue 12
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Source abstract

Abstract

Expansion microscopy (ExM) has gained increasing popularity for 3D ultrastructural imaging of cultured cells and tissue slices at nanoscale resolution using conventional microscopes via physical expansion of biological tissues. However, its application to collagen‐abundant thick tissues is still challenging. Herein, a new method, collagen ExM (ColExM), optimized for expanding tissues containing more than 70% collagen, is demonstrated. ColExM succeeds in 4.5‐fold linear expansion with minimal structural distortion of corneal and skin tissues. It is compatible with immunostaining, allowing super‐resolution visualization of 3D neural structures innervating hair follicles, corneas, and pancreatic tumors with high stromal collagen content. The method succeeds in identifying individual mitochondria and previously unrecognized dendritic spinelike structures of corneal nerves. It also enables fine mapping of structural rearrangement of tight junctions and actin cytoskeletons. Therefore, ColExM can facilitate the exploration of 3D nanoscale structures in collagen‐rich tissues.
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Research context

Nanomaterials

OpenAlex topics: Cellular Mechanics and Interactions · Angiogenesis and VEGF in Cancer · Optical Coherence Tomography Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering and Environmental Sciences National Tsing Hua University Hsinchu 30013 Taiwan

Brain Research Center National Tsing Hua University Hsinchu 30013 Taiwan

Department of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University Taipei 106216 Taiwan

Imaging Core, College of Medicine National Taiwan University Taipei 100225 Taiwan

Institute of Biomedical Engineering National Tsing Hua University Hsinchu 30013 Taiwan

Institute of Systems Neuroscience National Tsing Hua University Hsinchu 30013 Taiwan

Department of Chemistry National Tsing Hua University Hsinchu 30013 Taiwan

Department of Life Science, College of Life Science National Taiwan University Taipei 106216 Taiwan

Institute of Ecology and Evolutionary Biology, College of Life Science National Taiwan University Taipei 106216 Taiwan

Department of Medical Research and Department of Dermatology National Taiwan University Hospital Taipei 100225 Taiwan

Department of Dermatology, College of Medicine National Taiwan University Taipei 100225 Taiwan

Research Center for Developmental Biology and Regenerative Medicine National Taiwan University Taipei 106216 Taiwan

Funding

National Science and Technology Council · National Taiwan University · National Taiwan University Hospital · National Health Research Institutes

References (59)

Show source-provided references
1. 10.1038/nbt.362510.1038/nbt.3625 ↗
2. 10.1002/cpcb.5610.1002/cpcb.56 ↗
3. 10.1038/nmeth.383310.1038/nmeth.3833 ↗
4. 10.1126/science.126008810.1126/science.1260088 ↗
5. 10.1002/cpns.9610.1002/cpns.96 ↗
6. 10.1021/acsnano.0c0491510.1021/acsnano.0c04915 ↗
7. 10.1002/cbic.20200057110.1002/cbic.202000571 ↗
8. 10.1016/j.jneumeth.2021.10928510.1016/j.jneumeth.2021.109285 ↗
9. 10.1038/s41551-022-00912-310.1038/s41551-022-00912-3 ↗
10. 10.1038/s41598-018-28694-210.1038/s41598-018-28694-2 ↗
11. 10.3389/fmed.2018.0032210.3389/fmed.2018.00322 ↗
12. 10.1016/j.kint.2017.09.01910.1016/j.kint.2017.09.019 ↗
13. 10.1016/S0021-9258(18)56248-710.1016/S0021-9258(18)56248-7 ↗
14. Newsome D. A. · Invest. Ophthalmol. Vis. Sci. · 1981 · 20 · 738
15. 10.1101/cshperspect.a00497810.1101/cshperspect.a004978 ↗
16. 10.1021/acsnano.1c0960110.1021/acsnano.1c09601 ↗
17. 10.1038/nmeth.459310.1038/nmeth.4593 ↗
18. 10.1038/nmeth92910.1038/nmeth929 ↗
19. 10.1016/j.cell.2010.12.00210.1016/j.cell.2010.12.002 ↗
20. 10.1038/s41467-019-12715-310.1038/s41467-019-12715-3 ↗
21. 10.1111/j.1432-1033.1974.tb03671.x10.1111/j.1432-1033.1974.tb03671.x ↗
22. 10.1016/j.cub.2008.12.00510.1016/j.cub.2008.12.005 ↗
23. 10.1529/biophysj.106.08590210.1529/biophysj.106.085902 ↗
24. 10.1063/1.357819110.1063/1.3578191 ↗
25. 10.1016/S0161-6420(82)34746-610.1016/S0161-6420(82)34746-6 ↗
26. 10.1016/j.preteyeres.2019.05.00310.1016/j.preteyeres.2019.05.003 ↗
27. 10.7554/eLife.3888310.7554/eLife.38883 ↗
28. 10.1002/dvg.2075310.1002/dvg.20753 ↗
29. 10.1093/brain/awv35710.1093/brain/awv357 ↗
30. 10.1038/nrm.2016.8010.1038/nrm.2016.80 ↗
31. 10.1074/jbc.274.49.3517910.1074/jbc.274.49.35179 ↗
32. 10.1016/j.devcel.2020.07.02210.1016/j.devcel.2020.07.022 ↗
33. 10.5217/ir.2015.13.1.1110.5217/ir.2015.13.1.11 ↗
34. 10.1016/S0014-4835(03)00054-X10.1016/S0014-4835(03)00054-X ↗
35. 10.1046/j.1523-1747.1998.00209.x10.1046/j.1523-1747.1998.00209.x ↗
36. Noske W. · Ger. J. Ophthalmol. · 1994 · 3 · 253
37. 10.1083/jcb.58.2.39010.1083/jcb.58.2.390 ↗
38. 10.1161/01.STR.0000016405.06729.8310.1161/01.STR.0000016405.06729.83 ↗
39. 10.1152/jappl.1986.60.1.7110.1152/jappl.1986.60.1.71 ↗
40. 10.1152/ajpcell.1990.259.6.C97810.1152/ajpcell.1990.259.6.C978 ↗
41. 10.1002/1873-3468.1425210.1002/1873-3468.14252 ↗
42. 10.1073/pnas.080274110610.1073/pnas.0802741106 ↗
43. 10.1111/j.1749-6632.2009.04054.x10.1111/j.1749-6632.2009.04054.x ↗
44. 10.1152/ajpregu.1995.269.4.R84810.1152/ajpregu.1995.269.4.R848 ↗
45. 10.1007/s00232-001-0182-210.1007/s00232-001-0182-2 ↗
46. 10.1073/pnas.180702811510.1073/pnas.1807028115 ↗
47. 10.1038/srep0900410.1038/srep09004 ↗
48. 10.1155/2022/748600510.1155/2022/7486005 ↗
49. 10.1038/nmat478910.1038/nmat4789 ↗
50. 10.1126/sciadv.aav907510.1126/sciadv.aav9075 ↗
51. 10.1177/0022034521105381410.1177/00220345211053814 ↗
52. 10.1083/jcb.17.2.37510.1083/jcb.17.2.375 ↗
53. 10.1096/fj.02-0121fje10.1096/fj.02-0121fje ↗
54. 10.1126/sciadv.adf635810.1126/sciadv.adf6358 ↗
55. 10.1038/srep2904710.1038/srep29047 ↗
56. 10.1038/onc.2011.14610.1038/onc.2011.146 ↗
57. 10.1038/s41596-018-0117-310.1038/s41596-018-0117-3 ↗
58. 10.1016/j.brainres.2010.05.06110.1016/j.brainres.2010.05.061 ↗
59. 10.1007/978-1-60761-404-3_1110.1007/978-1-60761-404-3_11 ↗