DataVXSmall StructuresPaper
journal article·2024-12Open access

High‐Performance Cooperative DNA Nanodevice Enables Sensitive Circular RNA Imaging and Precise Tumor Growth Suppression

Ye Zhang, Siting Chen, Shihua Luo, Wenbin Li, Lifeng Zhang, Fei Lan, Yitong Zhu, Huijun Du, Ke Li, Chunchen Liu, Bo Situ, Bo Li, Xiaohui Yan

Small Structures· Volume 5· Issue 12
Publisher pageOpen full text
Source abstract

Abstract

The utility of circular RNAs (circRNAs) as emerging biomarkers and regulatory factors in medical diagnostics and therapeutics is hampered by the challenges associated with their sensitive detection and precise modulation. Herein, a high‐performance cooperative DNA nanodevice (HCDN) based on DNA tetrahedron‐confined catalytic DNA assembly reaction (DT‐CDA) that enables both imaging and regulation of circRNAs is developed. Activation of the DT‐CDA is contingent upon the presence of the target circRNA, which, together with a replicative fuel probe, catalyzes the sequential opening of additional DT‐CDAs. This cooperative exponential signal amplification with negligible background interference allows HCDN to effectively detect minute quantities of circRNAs. Employing circSATB2 as a model, the HCDN demonstrates substantial downregulation of Cyclin D1 (CCND1) mRNA and protein levels in cellular and in vivo models, thereby inhibiting tumor growth. The innovative design of HCDN sets the stage for a powerful methodology conducive to enhanced clinical diagnostics and biomolecule manipulation, thereby advancing the capabilities and applications of DNA nanotechnology.
Source: crossref · Quality: baseline

Research context

NanomaterialsCatalysis & surfaces

OpenAlex topics: Circular RNAs in diseases · MicroRNA in disease regulation · Extracellular vesicles in disease

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Laboratory Medicine Nanfang Hospital Medical Research Center of Nanfang Hospital School of Basic Medical Sciences Southern Medical University Guangzhou 510515 P. R. China

Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors Nanfang Hospital Southern Medical University Guangzhou 510515 P. R. China

Guangdong Provincial Key Laboratory of Proteomics School of Basic Medical Sciences Southern Medical University Guangzhou 510515 P. R. China

The Affiliated Qingyuan Hospital (Qingyuan People's Hospital) Guangzhou Medical University Qingyuan Guangdong 511518 P. R. China

Center for Clinical Laboratory Diagnosis and Research Affiliated Hospital of Youjiang Medical University for Nationalities Baise Guangxi 533000 P. R. China

Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions Affiliated Hospital of Youjiang Medical University for Nationalities Baise Guangxi 533000 P. R. China

References (56)

Show source-provided references
1. 10.1016/j.cell.2022.04.02110.1016/j.cell.2022.04.021 ↗
2. 10.1038/s41576-019-0158-710.1038/s41576-019-0158-7 ↗
3. 10.1038/nature1192810.1038/nature11928 ↗
4. 10.1038/nature1199310.1038/nature11993 ↗
5. 10.1038/s41380-022-01711-710.1038/s41380-022-01711-7 ↗
6. 10.1126/science.aam852610.1126/science.aam8526 ↗
7. 10.1016/j.canlet.2022.21581010.1016/j.canlet.2022.215810 ↗
8. 10.1002/hep.3079510.1002/hep.30795 ↗
9. 10.1111/jpi.1281310.1111/jpi.12813 ↗
10. 10.1038/s41392-020-00375-510.1038/s41392-020-00375-5 ↗
11. 10.1038/s41467-023-38232-y10.1038/s41467-023-38232-y ↗
12. 10.1093/bib/bbab41810.1093/bib/bbab418 ↗
13. 10.1186/s13073-019-0614-110.1186/s13073-019-0614-1 ↗
14. 10.1002/hep.3106810.1002/hep.31068 ↗
15. 10.1038/s41586-023-06834-710.1038/s41586-023-06834-7 ↗
16. 10.1038/s41587-021-00842-610.1038/s41587-021-00842-6 ↗
17. 10.1002/cac2.1227510.1002/cac2.12275 ↗
18. 10.1038/s41592-022-01487-210.1038/s41592-022-01487-2 ↗
19. 10.1038/nn.397510.1038/nn.3975 ↗
20. 10.1016/j.bios.2021.11350810.1016/j.bios.2021.113508 ↗
21. 10.1038/s41467-023-37020-y10.1038/s41467-023-37020-y ↗
22. 10.1016/j.tig.2022.02.00610.1016/j.tig.2022.02.006 ↗
23. 10.1002/inmd.1204610.1002/inmd.12046 ↗
24. 10.1002/INMD.2022000710.1002/INMD.20220007 ↗
25. 10.1002/inmd.1202910.1002/inmd.12029 ↗
26. 10.1038/s41571-021-00585-y10.1038/s41571-021-00585-y ↗
27. 10.1002/anie.20231040110.1002/anie.202310401 ↗
28. 10.1002/anie.20171202710.1002/anie.201712027 ↗
29. 10.1126/scirobotics.aam643110.1126/scirobotics.aam6431 ↗
30. 10.1126/sciadv.adi360210.1126/sciadv.adi3602 ↗
31. 10.1038/nnano.2009.8310.1038/nnano.2009.83 ↗
32. 10.1038/s41565-023-01348-910.1038/s41565-023-01348-9 ↗
33. 10.1038/s41565-023-01449-510.1038/s41565-023-01449-5 ↗
34. 10.1038/s41563-024-01865-510.1038/s41563-024-01865-5 ↗
35. 10.1038/s41551-022-01000-210.1038/s41551-022-01000-2 ↗
36. 10.1038/nbt.407110.1038/nbt.4071 ↗
37. 10.1038/s41596-020-0355-z10.1038/s41596-020-0355-z ↗
38. 10.1126/sciadv.adh850810.1126/sciadv.adh8508 ↗
39. 10.1038/s41570-024-00576-410.1038/s41570-024-00576-4 ↗
40. 10.1126/scirobotics.adf127410.1126/scirobotics.adf1274 ↗
41. 10.1016/j.bios.2023.11567710.1016/j.bios.2023.115677 ↗
42. 10.1002/advs.20230181410.1002/advs.202301814 ↗
43. 10.1002/advs.20230718810.1002/advs.202307188 ↗
44. 10.1016/j.mtbio.2022.10027610.1016/j.mtbio.2022.100276 ↗
45. 10.1126/sciadv.abl458910.1126/sciadv.abl4589 ↗
46. 10.1002/advs.20240051710.1002/advs.202400517 ↗
47. 10.1021/jacs.2c1150410.1021/jacs.2c11504 ↗
48. 10.1002/anie.20231130910.1002/anie.202311309 ↗
49. 10.1126/sciadv.aba938110.1126/sciadv.aba9381 ↗
50. Zhou L. · Sci. Adv. · 2020 · 6
51. 10.1186/s12951-023-02092-x10.1186/s12951-023-02092-x ↗
52. 10.18632/oncotarget.707110.18632/oncotarget.7071 ↗
53. 10.1186/s12943-020-01221-610.1186/s12943-020-01221-6 ↗
54. 10.1093/nar/gkz63810.1093/nar/gkz638 ↗
55. 10.1038/s41571-021-00507-y10.1038/s41571-021-00507-y ↗
56. 10.1021/jacs.1c0703610.1021/jacs.1c07036 ↗