The influence of alumina type on the immobilization of Ascorbate oxidase and ascorbic acid detection
Source abstract
Abstract
Two alumina types (trihydrate (T) and anhydrous (G)) were used for the immobilization of ascorbate oxidase (AO) by the adsorption procedure. The samples were characterized by FTIR analysis and EPR spectroscopy to confirm the presence of enzyme in the composite and the activity of immobilized enzyme. The samples of alumina with and without AO were used as modifer of carbon paste electrode and tested by Fe(CN6)3-/4- redox probe using cyclic voltammetry at different scan rates. The response of investigated electrodes toward ascorbic acid was tested in the Britton-Robinson buffer at pH range of 2 to 5. Direct detection of ascorbic acid was tested at G and G+AO electrodes and the calibration curves were constructed by square wave voltammetry under the optimized conditions. The immobilization of AO at anhydrous alumina increased the concentration range and the sensitivity from 0.00617 ?M/?A for G to 0.01693 ?M/?A for G+AO in the concentration range from 10 ?M to 200 ?M.
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Research context
OpenAlex topics: Electrochemical sensors and biosensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
University of Belgrade, Faculty of Physical Chemistry, Belgrade, Republic of Serbia
University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Catalysis and Chemical Engineering, Belgrade, Republic of Serbia
Funding
Ministry of Education, Science and Technological Development of the Republic of Serbia
References (32)
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