3D-printed bioactive scaffolds from nanosilicates and PEOT/PBT for bone tissue engineering
Source abstract
Abstract
Additive manufacturing (AM) has shown promise in designing 3D scaffold for regenerative medicine. However, many synthetic biomaterials used for AM are bioinert. Here, we report synthesis of bioactive nanocomposites from a poly(ethylene oxide terephthalate) (PEOT)/poly(butylene terephthalate) (PBT) (PEOT/PBT) copolymer and 2D nanosilicates for fabricating 3D scaffolds for bone tissue engineering. PEOT/PBT have been shown to support calcification and bone bonding ability in vivo, while 2D nanosilicates induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) in absence of osteoinductive agents. The effect of nanosilicates addition to PEOT/PBT on structural, mechanical and biological properties is investigated. Specifically, the addition of nanosilicate to PEOT/PBT improves the stability of nanocomposites in physiological conditions, as nanosilicate suppressed the degradation rate of copolymer. However, no significant increase in the mechanical stiffness of scaffold due to the addition of nanosilicates is observed. The addition of nanosilicates to PEOT/PBT improves the bioactive properties of AM nanocomposites as demonstrated in vitro. hMSCs readily proliferated on the scaffolds containing nanosilicates and resulted in significant upregulation of osteo-related proteins and production of mineralized matrix. The synergistic ability of nanosilicates and PEOT/PBT can be utilized for designing bioactive scaffolds for bone tissue engineering.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · 3D Printing in Biomedical Research · biodegradable polymer synthesis and properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA
Tissue Regeneration Department, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands
DTU Nanotech, Center for Intestinal Absorption and Transport of Biopharmaceuticals, Technical University of Denmark, 2800 Kgs, Lyngby, Denmark
Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitsingel 40, Maastricht, The Netherlands
Department of Materials Science, Texas A&M University, College Station, TX, USA and
Center for Remote Health Technologies and Systems, Texas A&M University, College Station, TX, USA
Funding
National Science Foundation · National Institute of Health · Dutch Technology Foundation
References (61)
Show source-provided references
1. Additive manufacturing of tissues and organs
2. Additive manufacturing of polymer melts for implantable medical devices and scaffolds
3. Advancing the field of 3D biomaterial printing
4. Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems
5. Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography
6. Rapid prototyping in tissue engineering: challenges and potential
7. A review of rapid prototyping techniques for tissue engineering purposes
8. Advanced bioinks for 3D printing: a materials science perspective
9. 3D fiber-deposited scaffolds for tissue engineering: influence of pores geometry and architecture on dynamic mechanical properties
10. Biofabrication: a guide to technology and terminology
11. Additively manufactured scaffolds for bone tissue engineering and the prediction of their mechanical behavior: a review
12. Geometric modeling of cellular materials for additive manufacturing in biomedical field: a review
13. Additive manufacturing of biologically-inspired materials
14. Porous PEOT/PBT scaffolds for bone tissue engineering: preparation, characterization, and in vitro bone marrow cell culturing
15. Enhanced bone marrow stromal cell adhesion and growth on segmented poly(ether ester)s based on poly(ethylene oxide) and poly(butylene terephthalate)
16. Design of segmented poly(ether ester) materials and structures for the tissue engineering of bone
17. Observations of the bone activity adjacent to unloaded dental implants coated with Polyactive or HA
18. Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
19. Interfacial behavior of PEO/PBT copolymers (Polyactive) in a calvarial system: an in vitro study
20. Use of bone-bonding hydrogel copolymers in bone: an in vitro and in vivo study of expanding PEO-PBT copolymers in goat femora
21. PEOT/PBT based scaffolds with low mechanical properties improve cartilage repair tissue formation in osteochondral defects
22. An open source image processing method to quantitatively assess tissue growth after non-invasive magnetic resonance imaging in human bone marrow stromal cell seeded 3D polymeric scaffolds
23. Evaluation of cartilage repair by mesenchymal stem cells seeded on a PEOT/PBT scaffold in an osteochondral defect
24. Adapted chondrogenic differentiation of human mesenchymal stem cells via controlled release of TGF-beta1 from poly(ethylene oxide)-terephtalate/poly(butylene terepthalate) multiblock scaffolds
25. Long-term bone tissue reaction to polyethylene oxide/polybutylene terephthalate copolymer (Polyactive) in metacarpophalangeal joint reconstruction
26. Ectopic bone formation in cell-seeded poly(ethylene oxide)/poly(butylene terephthalate) copolymer scaffolds of varying porosity
27. Three-dimensional fiber-deposited PEOT/PBT copolymer scaffolds for tissue engineering: influence of porosity, molecular network mesh size, and swelling in aqueous media on dynamic mechanical properties
28. Poly (dopamine) coating of 3D printed poly (lactic acid) scaffolds for bone tissue engineering
29. Recent advances in bone tissue engineering scaffolds
30. Three-dimensional printing of high-content graphene scaffolds for electronic and biomedical applications
31. Two-dimensional nanomaterials for biomedical applications: emerging trends and future prospects
32. Widespread changes in transcriptome profile of human mesenchymal stem cells induced by two-dimensional nanosilicates
33. Bioactive silicate nanoplatelets for osteogenic differentiation of human mesenchymal stem cells
34. Nanomaterials for engineering stem cell responses
35. Bioactive nanoengineered hydrogels for bone tissue engineering: a growth-factor-free approach
36. Nanoengineered osteoinductive and elastomeric scaffolds for bone tissue engineering
37. Shear-thinning nanocomposite hydrogels for the treatment of hemorrhage
38. Transparent, elastomeric and tough hydrogels from poly(ethylene glycol) and silicate nanoparticles
39. Preparation of laponite bioceramics for potential bone tissue engineering applications
40. Gradient nanocomposite hydrogels for interface tissue engineering
41. Injectable shear-thinning nanoengineered hydrogels for stem cell delivery
42. Amino-functionalized mesoporous bioactive glass for drug delivery
43. The role of surface charge on the uptake and biocompatibility of hydroxyapatite nanoparticles with osteoblast cells
44. In vivo and in vitro degradation of poly (ether ester) block copolymers based on poly (ethylene glycol) and poly (butylene terephthalate)
45. Distribution and viability of fetal and adult human bone marrow stromal cells in a biaxial rotating vessel bioreactor after seeding on polymeric 3D additive manufactured scaffolds
46. Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering
47. Bone tissue engineering using 3D printing
48. Design and 3D printing of scaffolds and tissues
49. Nanoclay-enriched poly(varepsilon-caprolactone) electrospun scaffolds for osteogenic differentiation of human mesenchymal stem cells
50. Model for cage formation in colloidal suspension of laponite
51. Viscosity and aggregation structure of nanocolloidal dispersions
52. Porosity of 3D biomaterial scaffolds and osteogenesis
53. Surface selective binding of nanoclay particles to polyampholyte protein chains
54. A small-angle neutron scattering study of adsorbed poly(ethylene oxide) on laponite
55. Elastomeric and mechanically stiff nanocomposites from poly (glycerol sebacate) and bioactive nanosilicates
56. Chemical stability of Laponite in aqueous media
57. The nature of laponite and its aqueous dispersions
58. Amphiphilic beads as depots for sustained drug release integrated into fibrillar scaffolds
59. Increased cell seeding efficiency in bioplotted three-dimensional PEOT/PBT scaffolds
60. 3D printing of scaffolds for tissue regeneration applications
61. A 3D bioprinting system to produce human-scale tissue constructs with structural integrity