Synergistic effects of fibronectin and bone morphogenetic protein on the bioactivity of titanium metal
Source abstract
Abstract
Abstract To improve the biological properties of bioactive titanium metal, recombinant human bone morphogenetic protein 2(rhBMP‐2) and fibronectin (Fn) were adsorbed on its surface solely or contiguously to modify the anodic oxidized titanium (AO‐Ti), acid–alkali‐treated titanium (AA‐Ti), and polished titanium (P‐Ti). It is found that the different bioactive titanium surface structures had great influence on protein adsorption. The adsorption amounts of BMP adsorbed solely and Fn/BMP adsorbed contiguously were AA‐Ti > P‐Ti > AO‐Ti, and that for Fn adsorbed solely was AA‐Ti ≈ P‐Ti > AO‐Ti. The conformation of proteins was changed remarkably after the adsorption. For BMP, the α‐helix decreased on AA‐Ti and stabilized on P‐Ti and AO‐Ti. For Fn, the β‐sheet on PT‐Ti and AA‐Ti increased significantly. For Fn/BMP, the percentage of β‐sheet on AA‐Ti increased, and that of α‐helix on all samples was stable. MSCs showed greater adhesion and spreading on Fn/BMP groups. MTT and Elisa tests showed that the synergistic effects of proteins made the cells proliferate and differentiate faster. It indicated both the surface structure and the synergistic effects of proteins could influence the biological properties of titanium metals. It provides research foundation for improving the biological properties of bioactive titanium metals by simultaneous application of several proteins. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2485–2498, 2017.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Orthopaedic implants and arthroplasty · Titanium Alloys Microstructure and Properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Engineering Research Center in Biomaterials Sichuan University Chengdu Sichuan 610064 China
National Engineering Research Center for Biomaterials Chengdu Sichuan 610064 China
Sichuan Guojia Biomaterials Co., Ltd Chengdu Sichuan 610064 China
Funding
National Key Program for Research and Development of China · National Nature Science Foundation of China · Key Program of Science & Technology Development of Sichuan Province, China · Key Program of Science & Technology Development of Chengdu, China
References (51)
Show source-provided references
1. 10.1016/S1003-6326(14)63163-010.1016/S1003-6326(14)63163-0 ↗
2. 10.1016/j.biomaterials.2016.01.01210.1016/j.biomaterials.2016.01.012 ↗
3. 10.1016/S0142-9612(03)00357-010.1016/S0142-9612(03)00357-0 ↗
4. 10.1016/j.apsusc.2009.11.07510.1016/j.apsusc.2009.11.075 ↗
5. 10.1016/S0142-9612(03)00626-410.1016/S0142-9612(03)00626-4 ↗
6. 10.1016/j.biomaterials.2016.01.00110.1016/j.biomaterials.2016.01.001 ↗
7. 10.1016/j.msec.2015.03.02810.1016/j.msec.2015.03.028 ↗
8. 10.1016/j.apsusc.2012.12.10510.1016/j.apsusc.2012.12.105 ↗
9. 10.3724/SP.J.1077.2011.0129910.3724/SP.J.1077.2011.01299 ↗
10. 10.1016/j.cplett.2011.05.03610.1016/j.cplett.2011.05.036 ↗
11. 10.1016/j.jconrel.2012.04.02110.1016/j.jconrel.2012.04.021 ↗
12. 10.1016/j.biomaterials.2008.10.04010.1016/j.biomaterials.2008.10.040 ↗
13. 10.1016/j.biomaterials.2011.06.06210.1016/j.biomaterials.2011.06.062 ↗
14. 10.1016/j.jconrel.2012.08.00410.1016/j.jconrel.2012.08.004 ↗
15. 10.1016/j.actbio.2016.01.04210.1016/j.actbio.2016.01.042 ↗
16. 10.1016/j.biomaterials.2013.08.05910.1016/j.biomaterials.2013.08.059 ↗
17. 10.1016/j.biomaterials.2015.12.00510.1016/j.biomaterials.2015.12.005 ↗
18. 10.1016/j.actbio.2015.12.00610.1016/j.actbio.2015.12.006 ↗
19. 10.1016/j.biomaterials.2010.09.00810.1016/j.biomaterials.2010.09.008 ↗
20. 10.1002/jbm.a.3476810.1002/jbm.a.34768 ↗
21. 10.1002/jbm.a.3571910.1002/jbm.a.35719 ↗
22. 10.1016/j.thromres.2015.08.00310.1016/j.thromres.2015.08.003 ↗
23. 10.1016/j.actbio.2016.10.01910.1016/j.actbio.2016.10.019 ↗
24. 10.1016/j.patbio.2011.10.00310.1016/j.patbio.2011.10.003 ↗
25. 10.1021/acsnano.6b0164910.1021/acsnano.6b01649 ↗
26. 10.1002/(SICI)1097-4636(199804)40:1<48::AID-JBM6>3.0.CO;2-R10.1002/(SICI)1097-4636(199804)40:1<48::AID-JBM6>3.0.CO;2-R ↗
27. 10.1002/jcp.2565810.1002/jcp.25658 ↗
28. 10.1016/j.colsurfb.2013.07.01610.1016/j.colsurfb.2013.07.016 ↗
29. 10.1038/sj.bjc.660091210.1038/sj.bjc.6600912 ↗
30. 10.1021/ja01189a08010.1021/ja01189a080 ↗
31. 10.1021/la202438u10.1021/la202438u ↗
32. 10.1016/j.biomaterials.2016.06.05210.1016/j.biomaterials.2016.06.052 ↗
33. 10.1016/j.msec.2016.08.03410.1016/j.msec.2016.08.034 ↗
34. 10.1083/jcb.88.3.63010.1083/jcb.88.3.630 ↗
35. 10.1016/j.bbabio.2007.06.00410.1016/j.bbabio.2007.06.004 ↗
36. 10.1016/j.apsusc.2012.08.01710.1016/j.apsusc.2012.08.017 ↗
37. 10.1111/j.1745-7270.2007.00320.x10.1111/j.1745-7270.2007.00320.x ↗
38. 10.1080/19420862.2015.108763610.1080/19420862.2015.1087636 ↗
39. 10.1371/journal.pone.013723310.1371/journal.pone.0137233 ↗
40. 10.1002/ppap.20150022810.1002/ppap.201500228 ↗
41. 10.1021/acs.langmuir.6b0222910.1021/acs.langmuir.6b02229 ↗
42. 10.1016/j.colsurfb.2016.01.06410.1016/j.colsurfb.2016.01.064 ↗
43. 10.1016/j.jmb.2013.03.01810.1016/j.jmb.2013.03.018 ↗
44. 10.1110/ps.06230510610.1110/ps.062305106 ↗
45. 10.1110/ps.421010210.1110/ps.4210102 ↗
46. 10.1016/0022-2836(92)91058-W10.1016/0022-2836(92)91058-W ↗
47. 10.1021/bi00483a00110.1021/bi00483a001 ↗
48. 10.1016/j.biomaterials.2015.10.01310.1016/j.biomaterials.2015.10.013 ↗
49. 10.1016/j.jcis.2015.01.08210.1016/j.jcis.2015.01.082 ↗
50. 10.1016/j.colsurfb.2016.10.00510.1016/j.colsurfb.2016.10.005 ↗
51. 10.1016/0166-6622(92)80164-W10.1016/0166-6622(92)80164-W ↗