Anti‐inflammatory effects of a titanium‐peroxy gel: Role of oxygen metabolites and apoptosis

Abstract Polymorphonuclear neutrophils (PMN) are among the first inflammatory cells to arrive at an implant interface, where they encounter with the foreign material and may produce reactive oxygen species (ROS). During the interaction between titanium and ROS, titanium‐peroxy (Ti‐peroxy) compounds may be formed. We used a Ti‐peroxy gel, made from titanium and hydrogen peroxide, to study the effects of Ti‐peroxy compounds on PMN. In the absence of serum, the Ti‐peroxy gel decreased the oxidative response of PMN to yeast and PMA and reduced PMN apoptosis without inducing necrosis. These effects could not be ascribed to the release of hydrogen peroxide from the Ti‐peroxy gel, because a steady‐state hydrogen peroxide producing system failed to mimic the effects of the gel. The effects were similarly unaffected when PMN were preincubated with β₂‐integrin antibodies, questioning the involvement of adhesion molecules. Nevertheless, when a filter was used to separate the Ti‐peroxy gel from the cells, the gel effect on PMN life span was abolished, pointing to a contact‐dependent mechanism. In the presence of serum, the Ti‐peroxy gel had no effect on the PMN oxidative response and life span, but appeared rather inert. In summary, this study demonstrates that the Ti‐peroxy gel has potentially anti‐inflammatory properties through a combined peroxide and physical contact effect, supporting the notion that interactions between titanium and inflammatory cells are responsible for the good performance of titanium in vivo. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 68A: 448–457, 2004