Bioadaptive nanorod array topography of hydroxyapatite and TiO₂ on Ti substrate to preosteoblast cell behaviors

Abstract Bioadaptive nanostructure coatings of hydroxyapatite (HAP) and TiO₂ on titanium (Ti) implants are essential for biomaterial‐tissue osteointegration. However, there is no specific report, so far, that focuses on the different influences of the two bioadaptive coatings on preosteoblast behaviors. Herein, adhesion, proliferation, and osteogenic potential of preosteoblast on HAP and TiO₂ coatings with nanorod array topography were studied. XRD, TEM, and SAED analysis indicated that rod‐like HAP nanoarray and anatase TiO₂ nanoarray coatings were fabricated successfully, and there was insignificant difference in roughness and fibronectin adsorption of the two coatings. Adhesion and proliferation of MC3T3‐E1 cells on the two coatings were of no significant difference, besides a larger projected area of the cells on HAP coating. MC3T3‐E1 cells cultured on the HAP coating displayed significantly higher expression of runt‐related transcription factor‐2 (Runx2), osteocalcin (OCN) and collagen type‐1 (Col I) after culture for 21 days compared with those on TiO₂ coating, except alkaline phosphatase (ALP). This study provides beneficial suggestion for intelligent selection of biocoatings.