Cytocompatibility studies of a biomimetic copolymer with simplified structure and high‐strength adhesion

Abstract The development of adhesives suitable for biomedical applications has been challenging given that these materials must exhibit sufficient adhesion strengths and biocompatibility. Biomimetic materials inspired by mussel adhesive proteins appear to contain many of the necessary characteristics for biomedical adhesives. In particular, poly[(3,4‐dihydroxystyrene)‐co‐styrene] has been shown to be a high strength adhesive material with bonding comparable to or even greater than several commercial glues. Herein, a thorough study on the cytocompatibility of this copolymer provides insights on the suitability of a mussel‐mimicking adhesive for applications development. The cytotoxicity of poly[(3,4‐dihydroxystyrene)‐co‐styrene] was evaluated through assessment of the viability, proliferation rate, and morphology of NIH/3T3 fibroblasts when cultured with copolymer extracts or directly in contact with the adhesive. After 1 and 3 days of culture, both the copolymer alone and copolymer cross‐linked with periodate exhibited minimal effects on cell viability. Likewise, cells cultured on the copolymer displayed proliferation rates and morphologies similar to cells on the poly‐l‐lysine control. These results indicate that poly[(3,4‐dihydroxystyrene)‐co‐styrene] is highly cytocompatible and therefore a promising material for use where biological contact is important. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 983–990, 2016.