Experimental investigation of pre-crosslinking methods and employing nano-hydroxyapatite powder on alginate/carboxymethyl cellulose hydrogel printability via 3D bioprinting
Source abstract
Abstract
Purpose Three-dimensional bioprinting (3D bioprinting) is used for repairing and regenerating living tissues due to its ease of use, cost-effectiveness and high precision in fabricating. Owing to their high biocompatibility, natural hydrogels are widely used as scaffold materials in bioprinting. However, the mechanical properties and low printability of hydrogels present a challenge. This study aims to introduce a composite hydrogel that exhibits excellent mechanical, biological and printability properties simultaneously. Design/methodology/approach Alginate (Alg), carboxymethyl cellulose (CMC) and nanohydroxyapatite (nHA) as suitable materials for 3D printing were used. Effect of material content and pre-crosslinking on various properties of these materials were investigated. Both quantitative and qualitative experiments were conducted to validate the biomaterial ink’s printability, its rheological characteristics, as well as its biological and mechanical properties. Findings Based on the analysis of the obtained experimental results from all mentioned tests, a hydrogel with a composition of 4% Alg, 2% CMC and 2% nHA with the pre-crosslinking process was selected as the preferred option. The results demonstrated that the selected material has good cell adhesion, wettability, degradation rate and 93% cell viability. Furthermore, compared to the composition of 4% Alg–2% CMC, the chosen material exhibited a 52% improvement in printability and a 55% improvement in compressive modulus. Originality/value A significant challenge in the field of 3D bioprinting is the development of scaffolds that possesses optimal mechanical, biological and printability characteristics simultaneously, essential for attaining tissue-like properties. Hence, this paper explores a novel nanocomposite hydrogel that demonstrates promising outcomes across all these aspects simultaneously.
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Research context
OpenAlex topics: 3D Printing in Biomedical Research · Additive Manufacturing and 3D Printing Technologies · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Amirkabir University of Technology , Tehran,
Amirkabir University of Technology Department of Mechanical Engineering, , Tehran,
References (55)
Show source-provided references
1. Structural, optical, thermal, and dielectric properties of carboxymethyl cellulose/sodium alginate blend/lithium titanium oxide nanoparticles: biocomposites for lithium-ion batteries applications10.1002/er.7877 ↗
2. Development of hydroxyapatite reinforced alginate–chitosan based printable biomaterial-ink10.1016/j.nanoso.2020.100630 ↗
3. Multilayer 3D bioprinting and complex mechanical properties of alginate-gelatin mesostructures10.1038/s41598-023-38323-2 ↗
4. Engineering alginate/carboxymethylcellulose scaffolds to establish liver cancer spheroids: evaluation of molecular variances between 2D and 3D models10.1016/j.ijbiomac.2023.128058 ↗
5. Development of a novel alginate-polyvinyl alcohol-hydroxyapatite hydrogel for 3D bioprinting bone tissue engineered scaffolds10.1002/jbm.a.36036 ↗
6. Inorganic nanomaterials in tissue engineering10.3390/pharmaceutics14061127 ↗
7. An overview of extrusion-based bioprinting with a focus on induced shear stress and its effect on cell viability10.1016/j.bprint.2020.e00093 ↗
8. Challenges and opportunities for the next generation of cardiovascular tissue engineering10.1038/s41592-022-01591-3 ↗
9. Drug–polymer interaction between glucosamine sulfate and alginate nanoparticles: FTIR, DSC and dielectric spectroscopy studies
10. Hydrogels for 3D bioprinting in tissue engineering and regenerative medicine: current progress and challenges10.18063/ijb.759 ↗
11. Alginate based scaffolds for cartilage tissue engineering: a review10.1080/00914037.2018.1562924 ↗
12. A study of the printability of alginate-based bioinks by 3D bioprinting for articular cartilage tissue engineering10.3390/polym14020354 ↗
13. Support grain architecture design for additive manufacturing10.1016/j.jmapro.2017.08.008 ↗
14. Rheological analysis of bio-ink for 3D bio-printing processes10.1016/j.jmapro.2022.02.048 ↗
15. Effect of process parameters on cellulose fiber alignment in bio-printing
16. 3D printability of alginate-carboxymethyl cellulose hydrogel10.3390/ma11030454 ↗
17. Eucalyptus bleached kraft pulp-ionic liquid inks for 3D printing of ionogels and hydrogels10.1016/j.carbpol.2023.120897 ↗
18. Development of a 3D-printed bioabsorbable composite scaffold with mechanical properties suitable for treating large
19. Physico-chemical modification of gelatine for the improvement of 3D printability of oxidized alginate-gelatine hydrogels towards cartilage tissue engineering10.1016/j.matdes.2021.109877 ↗
20. Preparation and properties of sodium carboxymethyl cellulose/sodium alginate/chitosan composite film10.3390/coatings8080291 ↗
21. Advancing scaffold porosity through a machine learning framework in extrusion based 3D bioprinting10.3389/fmats.2023.1337485 ↗
22. Influence of processing conditions on the properties of alginate solutions and wet edible calcium alginate coatings10.1016/j.lwt.2016.07.054 ↗
23. Preparation of food grade carboxymethyl cellulose from corn husk agrowaste10.1016/j.ijbiomac.2015.04.061 ↗
24. The fabrication of alginate–carboxymethyl cellulose-based composites and drug release profiles10.3390/polym14173604 ↗
25. Nano-hydroxyapatite-alginate-gelatin microcapsule as a potential osteogenic building block for modular bone tissue engineering
26. Printability–a key issue in extrusion-based bioprinting10.1016/j.jpha.2021.02.001 ↗
27. Printability of 3D printed hydrogel scaffolds: influence of hydrogel composition and printing parameters10.3390/app10010292 ↗
28. Physical modification of hybrid hydrogels to fabricate full-scale construct using three-dimensional bio-printing process10.1115/1.4055230 ↗
29. Enhanced mechanical strength and stretchable ionic conductive hydrogel with double-network structure for wearable strain sensing and energy harvesting10.1016/j.compscitech.2024.110732 ↗
30. Effect of bioink properties on printability and cell viability for 3D bioplotting of embryonic stem cells10.1088/1758-5090/8/3/035020 ↗
31. Bioprinting toward organ fabrication: challenges and future trends10.1109/TBME.2013.2243912 ↗
32. Solid-solid interfaces10.1016/B978-0-12-375049-5.00004-9 ↗
33. Studies on anticancerious and photocatalytic activity of carboxymethyl cellulose-cl-poly (lactic acid-co-itaconic acid)/ZnO-Ag nanocomposite10.1016/j.arabjc.2020.07.001 ↗
34. Simultaneous determination and optimization of titan yellow and reactive blue 4 dyes removal using chitosan@ hydroxyapatite nanocomposites10.1007/s10924-020-01982-7 ↗
35. Alginate and alginate composites for biomedical applications10.1016/j.ajps.2020.10.001 ↗
36. Influence of ionic crosslinkers (Ca2+/Ba2+/Zn2+) on the mechanical and biological properties of 3D bioplotted hydrogel scaffolds10.1080/09205063.2018.1433420 ↗
37. Tricomposite gelatin-carboxymethylcellulose-alginate bioink for direct and indirect 3D printing of human knee meniscal scaffold10.1016/j.ijbiomac.2021.11.184 ↗
38. Current status in the utilization of biobased polymers for 3D printing process: a systematic review of the materials, processes, and challenges10.1021/acsabm.0c01379 ↗
39. Physicochemical and biological behaviour of biogenic derived hydroxyapatite and carboxymethyl cellulose/sodium alginate biocomposite coating on Ti6Al4V alloy for biomedical applications10.1016/j.matchemphys.2020.123455 ↗
40. Smart biomaterials for tissue engineering of cartilage10.1016/j.injury.2008.01.036 ↗
41. A guide to polysaccharide-based hydrogel bioinks for 3D bioprinting applications10.3390/ijms23126564 ↗
42. Biocompatible synthetic polymers for tissue engineering purposes10.1021/acs.biomac.2c00047 ↗
43. Rheological behavior of fugitive organic inks for direct-write assembly10.1515/arh-2007-0001 ↗
44. Alginate and carboxymethyl cellulose in monolayer and bilayer films as wound dressings: effect of the polymer ratio10.1002/app.46941 ↗
45. Rheological analysis of low viscosity hydrogels for 3D bio-printing processes
46. Three-dimensional bioprinting of articular cartilage: a systematic review10.1177/1947603518809410 ↗
47. Hydrophilic cell-derived extracellular matrix as a niche to promote adhesion and differentiation of neural progenitor cells10.1039/C7RA08273H ↗
48. Homogeneous hydroxyapatite/alginate composite hydrogel promotes calcified cartilage matrix deposition with potential for three-dimensional bioprinting10.1088/1758-5090/aaf44a ↗
49. A three-dimensional porous hydroxyapatite nanocomposite scaffold with shape memory effect for bone tissue engineering10.1007/s10853-017-1807-x ↗
50. Fused deposition modeling of novel scaffold architectures for tissue engineering applications10.1016/S0142-9612(01)00232-0 ↗
51. Key advances of carboxymethyl cellulose in tissue engineering & 3D bioprinting applications10.1016/j.carbpol.2020.117561 ↗
52. Polymer fiber scaffolds for bone and cartilage tissue engineering10.1002/adfm.201903279 ↗
53. Design and fabrication of sodium alginate/carboxymethyl cellulose sodium blend hydrogel for artificial skin10.3390/gels7030115 ↗
54. Functional dyeing of cellulose macromolecule/synthetic fibers two-component fabrics with sustainable microbial prodigiosins10.1016/j.ijbiomac.2024.134964 ↗
55. Effects of CaCl2, HCl, acetic acid or citric acid on dynamic mechanical performances and physicochemical properties of sodium alginate edible films10.1016/j.fpsl.2022.100935 ↗