Phylogenetic position of two marine ciliates, Metanophrys similis and Pseudocohnilembus hargisi (Protozoa, Ciliophora Scuticociliatia), inferred from complete small subunit rRNA gene sequence *
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Abstract
Abstract The small subunit rRNA (SSrRNA) gene was sequenced for two marine scuticociliates Metanophrys similis and Pseudocohnilembus hargisi. The results show that this gene comprises 1763 and 1753 nucleotides in the two marine ciliates respectively. Metanophrys similis is phylogenetically closely related to the clade containing Mesanophrys carcini and Anophyroides haemophila, which branches basally to other species within the order Philasterida. Pseudocohnilembus hargisi groups with its congener, P. marinus, with strong bootstrap support. Paranophrys magna groups with the clade including Cohnilembus and Uronema, representing a sister clade to that containing the two Pseudocohnilembus species. *Supported by National Natural Science Foundation of China (Grant No. 30430090, 40376045) and the Darwin Initiative Program (Grant No. 14–015)
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OpenAlex topics: Protist diversity and phylogeny · Microbial Community Ecology and Physiology · Genomics and Phylogenetic Studies
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References (23)
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1. Petz, W. and Song, W. and Wilbert, N. (1995) Taxonomy and ecology of the ciliate fauna (Protozoa, Ciliophora) in the endopagial and pelagial of the Weddell Sea Antarctica Protistologica, 40, pp. 1 - 223.
2. Song, W. and Wilbert, N. (2002) Reinvestigations of three “well-known” marine scuticociliates: Uronemalla filificum (Kahl, 1931) nov. gen., nov comb., Pseudocohnilembus hargisi Evans and Thompson, 1964 and Cycilidium citrullus Cohn 1865, with description of the new genus Uronemella (Protozoa, Ciliophora, Scuticociliatida) Zool. Anz., 241, pp. 317 - 331.
3. Song, W. and Wilbert, N. (2000) Redefinition and redescription of some marine scuticociliates from China. I, with report of a new species Metanophrys sinensis nov. spec. (Ciliophora, Scuticociliatida) Zool. Anz., 239, pp. 45 - 74.
4. Song, W. and Shang, H. and Chen, Z. and Ma, H. (2002) Comparison of some closely related Metanophrys taxa with description of a new species Metanophrys similis nov. spec. (Ciliophora, Scuticociliatida) Europ. J. Protistol., 38, pp. 45 - 53.10.1078/0932-4739-00848 ↗
5. Morade, J. and Small, E. (1994) Morphology and stomatogenesis of Mesanophys pugettensis n. sp. (Scuticociliatida: Orchitophryidae), a faculative parasitic ciliate of the Dungeness crab, Cancer magister (Crustacea: Decapoda) Trans. Am. Microsc. Soc., 113, pp. 343 - 364.
6. Chen, Z. and Song, W. (2002) Phylogenetic position of Aspidisca steini and Euplotes vannus within the order Euplotida (Hypotrichia: Ciliophora) inferred from complete small subunit ribosomal RNA gene sequences Acta Protozool., 41, pp. 1 - 9.
7. Shang, H. and Chen, Z. and Song, W. (2002) Species seperation among seven Euplotes spp. (Protozoa, Ciliophora, Hypotrichida) using PCR RFLP analysis of nuclear ribosomal DNA J. Zool., 258, pp. 375 - 379.
8. Lynn, D. and Small, E. (1901) A revised classification of the Phylum Ciliophora doflein Rev. Soc. Mex. Hist. Nat., 1997, 47, pp. 65 - 78.
9. Stru¨der-Kypke, M. and Wright, A. and Fokin, S. and (2000) Phylogenetic relationships of the subclass Peniculia (Oligohymenophorea, Ciliophora) inferred from small subunit rRNA gene sequence J. Eukaryot. Microbiol., 47, pp. 419 - 429.10.1111/j.1550-7408.2000.tb00069.x ↗
10. Shang, H. and Song, W. and Warren, A. (2003) Phylogenetic positions of two ciliates, Paranophrys magna and Mesanophrys carcini (Protozoa, Ciliophora, Oligohymenophorea), within the subclass Scuticociliatia inferred from complete small subunit rRNA gene sequence Acta Protozool., 42, pp. 171 - 181.
11. Goggin, C. and Murphy, N. (2000) Conversation of sequence in the internal transcribed spacers and 5.8S ribosomal RNA among geographically separated isolates of parasitic scuticociliates Ciliophora, Orchitophryidae) Dis. Aquat. Org., 40, pp. 79 - 83.
12. Ragan, M. and Cawthorn, R. and Despres, B. and (1996) The lobster parasite Anophryoides haemophila (Scuticociliatida: Orchitophryidae) nuclear 18S rDNA sequence, phylogeny and detection using oligonucleotide primers J. Euk. Microbiol., 43, pp. 341 - 346.10.1111/j.1550-7408.1996.tb03998.x ↗
13. Felsenstein, J.(1995) PHYLIP: Phylogeny Inference Package, Version 3.57c. Seattle WA: Department of Genetics, University of Washington.
14. Kimura, M. (1980) A simple method of estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences J. Mol. Evol., 16, pp. 111 - 120.10.1007/BF01731581 ↗
15. Fitch, W. and Margoliash, E. (1967) Construction of phylogenetic trees Science, 155, pp. 279 - 284.
16. Saitou, N. and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees Mol. Biol. Evol., 4, pp. 406 - 425.
17. Kluge, A. and Farris, J. (1969) Quantitative phyletics and the evolution of anurans Syst. Zool., 18, pp. 1 - 32.
18. Sogin, M. and Elwood, H. (1986) Primary structure of the Paramecium tetraurelia small-subunit rRNA coding region: phylogenetic relationships within the Ciliophora J. Mol. Evol., 23, pp. 53 - 60.10.1007/BF02100998 ↗
19. Puytorac, D. and Grolière, C. and Roque, M. and (1974) A propos d, un cilieé Philasterina trouvé dans la cavité gé neérale du polycheète Nereis diversicolar O.F. Muller Protistologica, 10, pp. 101 - 111.
20. Evans, F. and Thompson, J. (1964) Pseudocohnilembidae n. fam., a hymenostome ciliate family containing one genus, Psudocohnilembus n.g., with three new species J. Protozool., 11, pp. 344 - 352.
21. Fernandez-Leborans, G. and Zaldumbide, M. (1984) Morphology of 3 species of free-living marine ciliate: Pseudocohnilembus cantabricus n.sp., Paralembus asturianus n.sp., Uronema castellonensis n.sp. (Ciliophora, Scuticociliatida) Arch, Protistenkd., 128, pp. 159 - 168.
22. Foissner, W. and Wilbert, N. (1981) A comparative study of the infraciliature and silverline system of the fresh-water scuticociliates Pseudocohnilembus Putrinus (Kahl, 1928) nov. comb., P. pusillus nov. comb., and the marine forms P. marinus Thompson, 1966 J. Protozool., 28, pp. 291 - 297.
23. Ma, H. and Song, W. and Hu, X. and (2003) Morphology and morphogenesis of Pseudocohnilembus hargisi Evans and Thompson, 1964 (Ciliophora, Scuticociliatida) J. Mar. Biol. Ass. UK, 83, pp. 399 - 405.10.1017/S0025315403007240h ↗