Tn5-mutagenesis and indentification of atr operon and trpE gene responsible for indole-3-acetic acid synthesis in Azospirillum brasilense Yu62*
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Abstract
Abstract To bring more information about synthesis of indole-3-acetic acid (IAA) from Azospirillum brasilense, a Tn5-insertion library of A. brasilense Yu62 was constructed and subjected to screening for IAA producing mutants. Two mutants with decreased IAA levels, named as A3 and A24, were isolated. The sequence analysis of loci tagged showed that the Tn5-1063a was located in the atrA gene encoidng GntR family transcriptional regulator and trpE gene encoding component I of anthranilate synthase respectively. At the same time, atrC were clustered in an operon. Mutagenesis and complementation studies showed that atrA and atrC were involved in IAA synthesis. IAA levels of trpE mutant and wild-type strain could be improved by adding anthranilate into the medium. *Supported by National High-Tech Project (Grant No. 2003AA241170), and the Major State Basic Research Development Programm of China(Grant No. 001CB108904)
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OpenAlex topics: Biofuel production and bioconversion · Plant-Microbe Interactions and Immunity · Microbial Metabolites in Food Biotechnology
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