Synthesis of Ciprofloxacin-Conjugated Gold Nanoparticles and their Study Antibacterial Effects on Growth Biofilm Formation Through Nebulizer Mask Against Respiratory Infection

Abstract An effective strategy has been identified as using antibiotic conjugated nanoparticles. The main goal of this research was to increase the biosynthesis of gold nanoparticles by conjugating it with ciprofloxacin. The optical, structural and biological properties of gold (Au) nanoparticles (NPs) and their conjugated form with ciprofloxacin (CIP@AuNPs). Using a simple and cost-effective method pulsed-laser ablation technique (PLAL). were studied using UV–VIS spectroscopy, X-ray diffraction(XRD), Transmission electron microscope (TEM), and Fourier transform infrared (FTIR), The findings demonstrated that TEM was used to determine the size and shape of nanoparticles with an average particle size of less than 20 nm. Investigated the antibacterial effects of AuNPs, antibiotic (ciprofloxacin) and CIP@AuNPs against two types of bacteria, namely, the gram-negative bacteria Klebsellia pneumonia and the gram-positive bacteria Streptococcus pneumonia. The well-diffusion method demonstrated that the effectiveness of (CIP@AuNPs) was greater than that of the antibiotic ciprofloxacin alone or Au NPs alone. Bacterial isolates tested for antibacterial susceptibility to the current study's antibiotics showed high resistance to them. The antimicrobial activity test revealed the largest inhibition zone in as Streptococcus pneumonia compared to Klebsiella pneumonia, with a more potent effect for Ciprofloxacin conjugated gold nanoparticles when compared with Ciprofloxacin alone and Au NPs alone. Further research was done on their capacity to prevent biofilm formation. This finding was supported by research using atomic force microscopy (AFM) to examine how Ciprofloxacin conjugated gold nanoparticles affected the development of bacterial biofilms in nebulizer masks. The combination of ciprofloxacin-conjugated gold nanoparticles may be used as a preservative of a Nebulizer mask. Through the prevent the formation of microbial biofilms in Nebulizer mask. The Ciprofloxacin conjugated gold nanoparticles also showed antioxidant activities by DPPH assay. Consequently, these investigations reveal that the prepared particles may be useful for the development of antioxidant drugs. Conclusion: Ciprofloxacin @Au nanoparticles are effective against multidrug-resistant bacteria in vitro and seem to be a good antibacterial agent.