Biomineralization Analysis and Hydration Acceleration Effect in Self-healing Concrete using Bacillus subtilis natto
Source abstract
Abstract
Since concrete with bacteria incorporated in the matrix shows promising results in initial studies, more research has focused on using bacteria for the strength and durability enhancement of concrete. Bacterial concentrations varying from different positions on the crack surface were recorded and evaluated in conjunction with the amount of self-healing product formed. The change in bacteria concentration with the survival time in concrete was investigated and lasted for two years. The degree of mineralization of calcium carbonate from the microbial activity is also closely related to the level of survival and reduction of bacterial concentrations in concrete compared to the initial amount. These processes were tracked and analyzed through phase composition analysis and microstructure analysis. The role of nucleation sites of bacteria for accelerating mineral deposition was also investigated. The change in the content of hydrated cementitious minerals can be seen in groups of samples with different bacteria regarding cracking age (7-90 days). The increase in C-S-H content in the bacterial samples at early cracking age was significant compared with the control group. The effect on healed crack parameters through microscopic observation contributed to supporting and demonstrating the hypothesis of the combination of the formation of the calcium carbonate crystals around the bacterial cell as crystallization nuclei and the promotion of hydration for C-S-H formation.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Microbial Applications in Construction Materials · Corrosion Behavior and Inhibition · Concrete and Cement Materials Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Silicate Materials, Faculty of Materials Technology, Ho Chi Minh City University of Technology (HCMUT), VNU-HCM, Ho Chi Minh City, Vietnam.
Department of Architecture, Tokyo University of Science, Tokyo, Japan.
References (53)
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1. 1) Abo-El-Enein, S. A., Ali, A. H., Talkhan, F. N. and Abdel-Gawwad, H. A., (2012). “Utilization of microbial induced calcite precipitation for sand consolidation and mortar crack remediation.” HBRC Journal, 8(3), 185-192.10.1016/j.hbrcj.2013.02.001 ↗
2. 2) Achal, V., Pan, X. and Özyurt, N., (2011). “Improved strength and durability of fly ash-amended concrete by microbial calcite precipitation.” Ecological Engineering, 37(4), 554-559.10.1016/j.ecoleng.2010.11.009 ↗
3. 3) Akın, A., (2022). “Mechanical and permeability properties on self-healing of cementitious composites effect of nano silica cure.” Structural Concrete, 23, 1907-1919.10.1002/suco.202100458 ↗
4. 4) ASTM, (2013). “Standard test method for measurement of rate of absorption of water by hydraulic-cement concretes (ASTM C1585/20).” West Conshohocken Pennsylvania, USA: ASTM International.
5. 5) Basaran, Z., (2013). “Biomineralization in cement based materials: Inoculation of vegetative cells.” Thesis (PhD). The University of Texas at Austin.
6. 6) Bentz, D. P., Ardani, A., Barrett, T., Jones, S. Z., Lootens, D., Peltz, M. A., Sato, T., Stutzman, P. E., Tanesi, J. and Weiss, W. J., (2015). “Multi-scale investigation of the performance of limestone in concrete.” Construction and Building Materials, 75, 1-10.10.1016/j.conbuildmat.2014.10.042 ↗
7. 7) Biswas, M., Majumdar, S., Chowdhury, T., Chattopadhyay, B., Mandal, S., Halder, U. and Yamasaki, S., (2010). “Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology.” Enzyme and Microbial Technology, 46(7), 581-587.10.1016/j.enzmictec.2010.03.005 ↗
8. 8) Bundur, Z. B., Kirisits, M. J. and Ferron, R. D., (2015). “Biomineralized cement-based materials: Impact of inoculating vegetative bacterial cells on hydration and strength.” Cement and Concrete Research, 67, 237-245.10.1016/j.cemconres.2014.10.002 ↗
9. 9) Cano-Medina, A., Jiménez-Islas, H., Dendooven, L., Herrera, R. P., González-Alatorre, G. and Escamilla-Silva, E. M., (2011). “Emulsifying and foaming capacity and emulsion and foam stability of sesame protein concentrates.” Food Research International, 44(3), 684-692.10.1016/j.foodres.2010.12.015 ↗
10. 10) Chen, X., Xu, L. and Wu, S., (2016). “Influence of pore structure on mechanical behavior of concrete under high strain rates.” Journal of Materials in Civil Engineering, 28(2), 04015110.10.1061/(ASCE)MT.1943-5533.0001380 ↗
11. 11) De Muynck, W., Cox, K., De Belie, N. and Verstraete, W., (2008). “Bacterial carbonate precipitation as an alternative surface treatment for concrete.” Construction and Building Materials, 22(5), 875-885.10.1016/j.conbuildmat.2006.12.011 ↗
12. 12) Dong, W., Li, W., Shen, L., Zhang, S. and Vessalas, K., (2021). “Integrated self-sensing and self-healing cementitious composite with microencapsulation of nano-carbon black and slaked lime.” Materials Letters, 282, 128834.10.1016/j.matlet.2020.128834 ↗
13. 13) Ghosh, S., Biswas, M., Chattopadhyay, B. D. and Mandal, S., (2009). “Microbial activity on the microstructure of bacteria modified mortar.” Cement and Concrete Composites, 31(2), 93-98.10.1016/j.cemconcomp.2009.01.001 ↗
14. 14) Holley, D., (2017). “General biology II: Organisms and ecology.” Indianapolis, Indiana, USA: Dog Ear Publishing.
15. 15) Hong, G. and Choi, S., (2017). “Rapid self-sealing of cracks in cementitious materials incorporating superabsorbent polymers.” Construction and Building Materials, 143, 366-375.10.1016/j.conbuildmat.2017.03.133 ↗
16. 16) Hong, G. and Choi, S., (2018). “Modeling rapid self-sealing of cracks in cementitious materials using superabsorbent polymers.” Construction and Building Materials, 164, 570-578.10.1016/j.conbuildmat.2018.01.017 ↗
17. 17) Huang, Y.-H., Chen, H.-J., Maity, J. P., Chen, C.-C., Sun, A.-C. and Chen, C.-Y., (2020). “Efficient option of industrial wastewater resources in cement mortar application with river-sand by microbial induced calcium carbonate precipitation.” Scientific Reports, 10(1), 1-11.10.1038/s41598-020-62666-9 ↗
18. 18) Huynh, N. N. T., Imamoto, K. and Kiyohara, C., (2020). “Eco-friendly technique on nutrient sources and capsulation for bacteria-based self-healing concrete.” Proceedings of the Japan Concrete Institute, 42(1), 1288-1293.
19. 19) Huynh, N. N. T., Phuong, N. M., Toan, N. P. A. and Son, N. K., (2017). “Bacillus subtilis HU58 immobilized in micropores of diatomite for using in self-healing concrete.” Procedia Engineering, 171, 598-605.10.1016/j.proeng.2017.01.385 ↗
20. 20) Jang, I., Son, D., Kim, W., Park, W. and Yi, C., (2020). “Effects of spray-dried co-cultured bacteria on cement mortar.” Construction and Building Materials, 243, 118206.10.1016/j.conbuildmat.2020.118206 ↗
21. 21) Jones, R. J., Delesky, E. A., Cook, S. M., Cameron, J. C., Hubler, M. H. and Srubar, W. V., (2022). “Engineered living materials for construction.” In: W. V. Srubar, Ed. Engineered Living Materials. Cham, Switzerland: Springer, 187-216.10.1007/978-3-030-92949-7_7 ↗
22. 22) Jonkers, H. M., (2007). “Self healing concrete: A biological approach.” In: S. Zwaag, Ed. Self healing Materials. Dordrecht, Germany: Springer, 195-204.10.1007/978-1-4020-6250-6_9 ↗
23. 23) Jonkers, H. M., (2011). “Bacteria-based self-healing concrete.” Heron Journal, 56, 1-12.
24. 24) Jonkers, H. M. and Schlangen, E., (2008). “Development of a bacteria-based self healing concrete.” In: J. C. Walraven and D. Stoelhorst, Eds. Tailor Made Concrete Structures. London: Taylor & Francis Group, 425-430.10.1201/9781439828410.ch72 ↗
25. 25) Jonkers, H. M., Thijssen, A., Muyzer, G., Copuroglu, O. and Schlangen, E., (2010). “Application of bacteria as self-healing agent for the development of sustainable concrete.” Ecological Engineering, 36(2), 230-235.10.1016/j.ecoleng.2008.12.036 ↗
26. 26) Khalifa, S. F., (2016). “Fabrication and characterization of antibacterial herbal drug loaded polylactic acid/cellulose acetate nanocomposite nanofibers for wound dressing applications.” Thesis (Master’s). The American University of Cairo.
27. 27) Lindsay, D., Brözel, V. S. and Von Holy, A., (2006). “Biofilm-spore response in Bacillus cereus and Bacillus subtilis during nutrient limitation.” Journal of Food Protection, 69(5), 1168-1172.10.4315/0362-028X-69.5.1168 ↗
28. 28) Mitchell, J. K. and Santamarina, J. C., (2005). “Biological considerations in geotechnical engineering.” Journal of Geotechnical and Geoenvironmental Engineering, 131(10), 1222-1233.10.1061/(ASCE)1090-0241(2005)131:10(1222) ↗
29. 29) Nain, N., Surabhi, R., Yathish, N. V., Krishnamurthy, V., Deepa, T. and Tharannum, S., (2019). “Enhancement in strength parameters of concrete by application of Bacillus bacteria.” Construction and Building Materials, 202, 904-908.10.1016/j.conbuildmat.2019.01.059 ↗
30. 30) Naveed, M., Duan, J., Uddin, S., Suleman, M., Hui, Y. and Li, H., (2020). “Application of microbially induced calcium carbonate precipitation with urea hydrolysis to improve the mechanical properties of soil.” Ecological Engineering, 153, 105885.10.1016/j.ecoleng.2020.105885 ↗
31. 31) Nguyen, H. N. T., Nguyen-Phung, A. T. and Nguyen, K. S., (2016). “Long-term mechanical performance of microbial modified concrete.” In: H. Q. Le, T. T. Le, T. V. Nguyen and L. N. Nguyen, Eds. Proc. 7th International Conference of Asian Concrete Federation - Sustainable Concrete for Now and the Future, Hanoi, Vietnam 30 October - 03 November 2016. Hanoi: Construction Publishing House, Paper No. 83.
32. 32) Putri, P. Y., Ujike, I. and Kawaai, K., (2019). “Application of bio-based material for concrete repair: Case study leakage on parallel concrete slab.” MATEC Web of Conferences, 258, 01013.10.1051/matecconf/201925801013 ↗
33. 33) Ramachandran, S. K., Ramakrishnan, V. and Bang, S. S., (2001). “Remediation of concrete using micro-organisms.” ACI Materials Journal, 98(1), 3-9.10.14359/10154 ↗
34. 34) Rao, M., Reddy, V. S., Hafsa, M., Veena, P. and Anusha, P., (2013). “Bioengineered concrete - A sustainable self-healing construction material.” Research Journal of Engineering Sciences, 2(6), 45-51.
35. 35) Rao, M. S., Reddy, V. S. and Sasikala, C., (2017). “Performance of microbial concrete developed using Bacillus subtilus JC3.” Journal of The Institution of Engineers (India): Series A, 98(4), 501-510.10.1007/s40030-017-0227-x ↗
36. 36) Reddy, S. V., Satya, A. K., Rao, S. M. and Azmatunnisa, M., (2012). “A biological approach to enhance strength and durability in concrete structures.” International Journal of Advances in Engineering & Technology, 4(2), 392-399.
37. 37) Seifan, M. and Berenjian, A., (2019). “Microbially induced calcium carbonate precipitation: A widespread phenomenon in the biological world.” Applied microbiology and Biotechnology, 103(12), 4693-4708.10.1007/s00253-019-09861-5 ↗
38. 38) Shim, Y., Hong, G. and Choi, S., (2018). “Autogenous healing of early-age cementitious materials incorporating superabsorbent polymers exposed to wet/dry cycles.” Materials, 11(12), 2476.10.3390/ma11122476 ↗
39. 39) Silva, F., Boon, N. and Verstraete, W., (2014). “The industrial markets of sporulating bacteria.” Academicia, 4(5), 81-94.
40. 40) Singh, P., Kaur, I. and Singh, N., (2020). “A review of different bacteria carriers used in self-healing mechanism.” Materials Today: Proceedings, 32(4), 955-960.10.1016/j.matpr.2020.05.100 ↗
41. 41) Snoeck, D., Dewanckele, J., Cnudde, V. and De Belie, N., (2016). “X-ray computed microtomography to study autogenous healing of cementitious materials promoted by superabsorbent polymers.” Cement and Concrete Composites, 65, 83-93.10.1016/j.cemconcomp.2015.10.016 ↗
42. 42) Souradeep, G. and Kua, H. W., (2016). “Encapsulation technology and techniques in self-healing concrete.” Journal of Materials in Civil Engineering, 28(12), 04016165.10.1061/(ASCE)MT.1943-5533.0001687 ↗
43. 43) Stefanidou, M., Tsampali, E., Karagiannis, G., Amanatiadis, S., Ioakim, A. and Kassavetis, S., (2021). “Techniques for recording self-healing efficiency and characterizing the healing products in cementitious materials.” Material Design & Processing Communications, 3, e0166.10.1002/mdp2.166 ↗
44. 44) Stülke, J. and Hillen, W., (2000). “Regulation of carbon catabolism in Bacillus species.” Annual Reviews in Microbiology, 54(1), 849-880.10.1146/annurev.micro.54.1.849 ↗
45. 45) Torres, A. R., Martinez-Toledo, M. V., Gonzalez-Martinez, A., Gonzalez-Lopez, J., Martin-Ramos, D. and Rivadeneyra, M. A., (2013). “Precipitation of carbonates by bacteria isolated from wastewater samples collected in a conventional wastewater treatment plant.” International Journal of Environmental Science and Technology, 10(1), 141-150.10.1007/s13762-012-0084-0 ↗
46. 46) Ujike, I., Kubo, F., Kawaai, K. and Okazaki, S., (2014). “Influencing factors affecting microbial metabolic processes of bio materials used for leaking repairs.” In: M. Grantham, P. A. M. Basheer, B. Magee and M. Soutsos, Eds. Concrete Solutions - Proc. 5th International Conferences on Concrete Repair, Belfast, UK 1-3 September 2014. London: CRC Press, 127-133.10.1201/b17394-21 ↗
47. 47) Vicente, M. A., Mínguez, J. and González, D. C., (2019). “Variation of the pore morphology during the early age in plain and fiber-reinforced high-performance concrete under moisture-saturated curing.” Materials, 12(6), 975.10.3390/ma12060975 ↗
48. 48) Wang, J., (2013). “Self-healing concrete by means of immobilized carbonate precipitating bacteria.” Thesis (PhD). Ghent University.
49. 49) Wang, J. Y., Soens, H., Verstraete, W. and De Belie, N., (2014). “Self-healing concrete by use of microencapsulated bacterial spores.” Cement and Concrete Research, 56, 139-152.10.1016/j.cemconres.2013.11.009 ↗
50. 50) Whiffin, V. S., (2004). “Microbial CaCO3 precipitation for the production of biocement.” Thesis (PhD). Murdoch University.
51. 51) Xu, J., Yao, W. and Jiang, Z., (2014). “Non-ureolytic bacterial carbonate precipitation as a surface treatment strategy on cementitious materials.” Journal of Materials in Civil Engineering, 26(5), 983-991.10.1061/(ASCE)MT.1943-5533.0000906 ↗
52. 52) Xue, C., Li, W., Qu, F., Sun, Z. and Shah, S. P., (2020). “Self-healing efficiency and crack closure of smart cementitious composite with crystalline admixture and structural polyurethane.” Construction and Building Materials, 260, 119955.10.1016/j.conbuildmat.2020.119955 ↗
53. 53) Ylmén, R., Jäglid, U., Steenari, B.-M. and Panas, I., (2009). “Early hydration and setting of Portland cement monitored by IR, SEM and Vicat techniques.” Cement and Concrete Research, 39(5), 433-439.10.1016/j.cemconres.2009.01.017 ↗