Application of Bio-remediation with Bacillus megaterium for Crack Repair at Low Temperature
Source abstract
Abstract
Less calcium carbonate in low temperature significantly restricts the application of microbial-induced carbonate precipitation (MICP) technology. Bacillus megaterium was chosen and calcification precipitation tests were studied via varying experimental conditions. Moreover, crack repair tests based on MICP were performed, using the viable cell concentration, sonic time values, unconfined compressive strength and productive rates for calcium carbonate as indicators to evaluate repair effects. Results show that adding nutrients to the gelling solution and determining the optimal reaction conditions can increase production rates for calcium carbonate in low temperature. However, for undomesticated and domesticated B. megaterium, the optimum calcification reaction conditions were different. With MICP technology, cracks were successfully sealed by calcium carbonate in low temperature and the strengths of specimens with different crack widths all recovered. The smaller the crack width, the better was the microbial utilization efficiency but worse repair uniformity. The repair effect with undomesticated B. megaterium was worst, and the specimens repaired by the method, domesticated B. megaterium and adding urea, had the best repair effect. Therefore, results demonstrated the feasibility of MICP crack repair at low temperature, which lay the foundation for subsequent practical engineering application with B. megaterium in low temperature.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Microbial Applications in Construction Materials · Bacterial biofilms and quorum sensing · Corrosion Behavior and Inhibition
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Geotechnical Engineering, Southeast University, Nanjing, Jiangsu, China, 210096.
References (52)
Show source-provided references
1. 1) Achal, V., (2010). “Microbial remediation of defects in building materials and structures.” Thesis (PhD). Thapar University, India.
2. 2) Achal, V., Mukherjee, A., Kumari, D. and Zhang, Q., (2015). “Biomineralization for sustainable construction - a review of processes and applications.” Earth-Science Reviews, 148, 1-17.10.1016/j.earscirev.2015.05.008 ↗
3. 3) Achal, V., Pan, X. and Özyurt, N., (2011). “Improved strength and durability of fly ash-amended concrete by microbial calcite precipitation.” Ecological Engineering, 37(4), 554-559.10.1016/j.ecoleng.2010.11.009 ↗
4. 4) Al Qabany, A. and Soga, K., (2013). “Effect of chemical treatment used in MICP on engineering properties of cemented soils.” Géotechnique, 63 (4), 331-339.10.1680/geot.SIP13.P.022 ↗
5. 5) Banks, E. D., Taylor, N. M., Gulley, J., Lubbers, B. R., Giarrizzo, J. G., Bullen, H. A., Hoehler, T. M. and Barton, H. A., (2010). “Bacterial calcium carbonate precipitation in cave environments: a function of calcium homeostasis.” Geomicrobiology Journal, 27(5), 444-454.10.1080/01490450903485136 ↗
6. 6) Bundur, Z. B., Kirisits, M. J. and Ferron, R. D., (2015). “Biomineralized cement-based materials: impact of inoculating vegetative bacterial cells on hydration and strength.” Cement and Concrete Research, 67, 237-245.10.1016/j.cemconres.2014.10.002 ↗
7. 7) Cacchio, P., Ercole, C., Cappuccio, G. and Lepidi, A., (2003). “Calcium carbonate precipitation by bacterial strains isolated from a limestone cave and from a loamy soil.” Geomicrobiology Journal, 20(2), 85-98.10.1080/01490450303883 ↗
8. 8) Choi, S. G., Wang, K., Wen, Z. and Chu. J., (2017). “Mortar crack repair using microbial induced calcite precipitation method.” Cement and Concrete Composites, 83, 209-221.10.1016/j.cemconcomp.2017.07.013 ↗
9. 9) Chu, J., Stabnikova, V. and Ivanova, V., (2012). “Microbially induced calcium carbonate precipitation on surface or in the bulk of soil.” Geomicrobiology Journal, 29(6), 544-549.10.1080/01490451.2011.592929 ↗
10. 10) Ciurli, S., Marzadori, C., Benini, S., Deiana, S. and Gessa, C., (1996). “Urease from the soil bacterium Bacillus pasteurii: immobilization on Ca-polygalacturonate.” Soil Biology and Biochemistry, 28(6), 811-817.10.1016/0038-0717(96)00020-X ↗
11. 11) Cuthbert, M. O., McMillan, L. A., Handley-Sidhu, S., Riley, M. S., Tobler, D. J. and Phoenix, V. R., (2013). “A field and modeling study of fractured rock permeability reduction using microbially induced calcite precipitation.” Environmental Science and Technology, 47 (23), 13637-13643.10.1021/es402601g ↗
12. 12) Danjo, T. and Kawasaki, S., (2014). “Formation mechanisms of beachrocks in Okinawa and Ishikawa, Japan, with a focus on cements.” Materials Transactions, 55(3), 493-500.10.2320/matertrans.M-M2013844 ↗
13. 13) DeJong, J. T., Mortensen B. M., Martinez B. C. and Nelson, D. C., (2010). “Bio-mediated soil improvement.” Ecological Engineering, 36(2), 197-210.10.1016/j.ecoleng.2008.12.029 ↗
14. 14) De Muynck, W., De Belie, N. and Verstraete, W., (2010). “Microbial carbonate precipitation in construction materials: a review.” Ecological Engineering, 36(2), 118-136.10.1016/j.ecoleng.2009.02.006 ↗
15. 15) Dhami, N. K., Reddy, M. S. and Mukherjee, A., (2012). “Improvement in strength properties of ash bricks by bacterial calcite.” Ecological Engineering, 39, 31-35.10.1016/j.ecoleng.2011.11.011 ↗
16. 16) Dupraz, S., Parmentier, M., Ménez, B. and Guyot, F., (2009). “Experimental and numerical modeling of bacterially induced pH increase and calcite precipitation in saline aquifers.” Chemical Geology, 265(1-2), 44-53.10.1016/j.chemgeo.2009.05.003 ↗
17. 17) Fredrickson, J. K. and Fletcher, M., (2001). “Subsurface microbiology and biogeochemistry.” New York: Wiley-Liss Inc.
18. 18) Fukue, M., Ono, S. and Sato, Y., (2011). “Cementation of sands due to microbiologically-induced carbonate precipitation.” Soils and Foundations, 51(1), 83-93.10.3208/sandf.51.83 ↗
19. 19) Ghosh, P., Mandal, S., Chattopadhyay, B. D. and Pal, S., (2005). “Use of microorganism to improve the strength of cement mortar.” Cement and Concrete Research, 35(10), 1980-1983.10.1016/j.cemconres.2005.03.005 ↗
20. 20) Ivanov, V. and Chu, J., (2008). “Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ.” Reviews in Environmental Science and Bio/Technology, 7(2), 139-153.10.1007/s11157-007-9126-3 ↗
21. 21) Khaliq, W. and Ehsan, M. B., (2016). “Crack healing in concrete using various bio influenced self-healing techniques.” Construction and Building Materials, 102, 349-357.10.1016/j.conbuildmat.2015.11.006 ↗
22. 22) Khan, J. A., (2011). “Biodegradation of azo dye by moderately halotolerant Bacillus megaterium and study of enzyme azoreductase involved in degradation.” Advanced Biotech, 10(7), 21-27.
23. 23) Khan, M. N. H., Amarakoon, G. G. N. N., Shimazaki, S. and Kawasaki, S., (2015). “Coral sand solidification test based on microbially induced carbonate precipitation using ureolytic bacteria.” Materials Transactions, 56(10), 1725-1732.10.2320/matertrans.M-M2015820 ↗
24. 24) Krulwich, T. A., Sachs, G. and Padan, E., (2011). “Molecular aspects of bacterial pH sensing and homeostasis.” Nature Reviews Microbiology, 9(5), 330-343.10.1038/nrmicro2549 ↗
25. 25) Kumari, D., Pan, X., Lee, D. J. and Achal, V., (2014). “Immobilization of cadmium in soil by microbially induced carbonate precipitation Exiguobacterium undae at low temperature.” International Biodeterioration and Biodegradation, 94, 98-102.10.1016/j.ibiod.2014.07.007 ↗
26. 26) Lian, B., Hu, Q., Chen, J., Ji, J. and Teng, H. H., (2006). “Carbonate biomineralization induced by soil bacterium Bacillus megaterium.” Geochimica et Cosmochimica Acta, 70(22), 5522-5535.10.1016/j.gca.2006.08.044 ↗
27. 27) Lors, C., Ducasse-Lapeyrusse, J., Gagné, R. and Damidot, D., (2017). “Microbiologically induced calcium carbonate precipitation to repair microcracks remaining after autogenous healing of mortars.” Construction and Building Materials, 141, 461-469.10.1016/j.conbuildmat.2017.03.026 ↗
28. 28) Montoya, B., DeJong, J. and Boulanger, R., (2013). “Dynamic response of liquefiable sand improved by microbial-induced calcite precipitation.” Géotechnique, 63 (4), 302-312.10.1680/geot.SIP13.P.019 ↗
29. 29) Nemati, M., Greene, E. A. and Voordouw, G., (2005). “Permeability profile modification using bacterially formed calcium carbonate: comparison with enzymic option.” Process Biochemistry, 40(2), 925-933.10.1016/j.procbio.2004.02.019 ↗
30. 30) Okwadha, G. D. and Li, J., (2010). “Optimum conditions for microbial carbonate precipitation.” Chemosphere, 81(9), 1143-1148.10.1016/j.chemosphere.2010.09.066 ↗
31. 31) Ramachandran, S. K., Ramakrishnan, V. and Bang, S. S., (2001). “Remediation of concrete using microorganisms.” ACI Materials Journal, 98(1), 3-9.
32. 32) RILEM, (2013). “State-of-the-art report of RILEM technical committee 221-SHC, vol. 11: Self-healing phenomena in cement-based materials.” M. De Rooij, K. Van Tittelboom, N. De Belie and E. Schlangen, Eds. Dordrecht: Springer Science and Business Media.
33. 33) Soon, N. W., Lee, L. M., Khun, T. C. and Ling, S. H., (2014). “Factors affecting improvement in engineering properties of residual soil through microbial-induced calcite precipitation.” Journal of Geotechnical and Geoenvironmental Engineering, 140(5), 04014006.10.1061/(ASCE)GT.1943-5606.0001089 ↗
34. 34) Stocks-Fischer, S., Galinat, J. K. and Bang, S. S., (1999). “Microbiological precipitation of CaCO3.” Soil Biology and Biochemistry, 31(11), 1563-1571.10.1016/S0038-0717(99)00082-6 ↗
35. 35) Suer, P., Hallberg, N., Carlsson, C., Bendz, D. and Holm, G., (2009). “Biogrouting compared to jet grouting: environmental (LCA) and economical assessment.” Journal of Environmental Science and Health, Part A, 44(4), 346-353.10.1080/10934520802659679 ↗
36. 36) Sun, X., Miao, L. C., Tong, T. Z. and Wang, C. C., (2018a). “Improvement of microbial-induced calcium carbonate precipitation technology for sand solidification.” Journal of Materials in Civil Engineering, 30(11), 04018301.10.1061/(ASCE)MT.1943-5533.0002507 ↗
37. 37) Sun, X., Miao, L., Wu, L., Wang, C. and Chen, R., (2018b). “The method of repairing microcracks based on microbiologically induced calcium carbonate precipitation.” Advances in Cement Research, 32(6), 262-272.10.1680/jadcr.18.00121 ↗
38. 38) Sun, X., Miao, L., Tong, T. and Wang, C., (2019a). “Study of the effect of temperature on microbially induced carbonate precipitation.” Acta Geotechnica, 14(3), 627-638.10.1007/s11440-018-0758-y ↗
39. 39) Sun, X., Miao, L. and Wang, C., (2019b). “Glucose addition improves the bio-remediation efficiency for crack repair.” Materials and Structures, 52(6), 1-18.10.1617/s11527-019-1410-5 ↗
40. 40) Sun, X., Miao, L., Wu, L. and Chen, R., (2019c). “The improvement of bio-cementation at low temperature based on Bacillus megaterium.” Applied Microbiology and Biotechnology, 103(17), 7191-7202.10.1007/s00253-019-09986-7 ↗
41. 41) Tziviloglou, E., Wiktor, V., Jonkers, H. M. and Schlangen E., (2016). “Bacteria-based self-healing concrete to increase liquid tightness of cracks.” Construction and Building Materials, 122, 118-125.10.1016/j.conbuildmat.2016.06.080 ↗
42. 42) Van Tittelboom, K., De Belie, N., De Muynck, W. and Verstraete, W., (2010). “Use of bacteria to repair cracks in concrete.” Cement and Concrete Research, 40(1), 157-166.10.1016/j.cemconres.2009.08.025 ↗
43. 43) Van Paassen, L. A., (2009). “Biogrout, ground improvement by microbial induced carbonate precipitation.” Thesis (PhD). Delft University of Technology, Delft, The Netherlands.
44. 44) Wang, J. Y., De Belie, N. and Verstraete, W., (2012). “Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete.” Journal of Industrial Microbiology and Biotechnology, 39(4), 567-577.10.1007/s10295-011-1037-1 ↗
45. 45) Wright, D. T. and Oren, A., (2005). “Nonphotosynthetic bacteria and the formation of carbonates and evaporites through time.” Geomicrobiology Journal, 22(1-2), 27-53.10.1080/01490450590922532 ↗
46. 46) Whiffin, V. S., (2004). “Microbial CaCO3 precipitation for the production of biocement.” Thesis (PhD). Murdoch University, Perth, Australia.
47. 47) Whiffin, V. S., van Paassen, L. A. and Harkes, M. P., (2007). “Microbial carbonate precipitation as a soil improvement technique.” Geomicrobiology Journal, 24(5), 417-423.10.1080/01490450701436505 ↗
48. 48) Wiktor, V. and Jonkers, H. M., (2011). “Quantification of crack-healing in novel bacteria-based self-healing concrete.” Cement and Concrete Composites, 33(7), 763-770.10.1016/j.cemconcomp.2011.03.012 ↗
49. 49) Yang, Z., Hollar, J., He, X. and Shi, X., (2011). “A self-healing cementitious composite using oil core/silica gel shell microcapsules.” Cement and Concrete Composites, 33(4), 506-512.10.1016/j.cemconcomp.2011.01.010 ↗
50. 50) Yu, X. and Jiang, J., (2018). “Mineralization and cementing properties of bio-carbonate cement, bio-phosphate cement, and bio-carbonate/phosphate cement: a review.” Environmental Science and Pollution Research, 25(22), 21483-21497.10.1007/s11356-018-2143-7 ↗
51. 51) Yu, X., Qian, C. and Sun, L., (2018). “The influence of the number of injections of bio-composite cement on the properties of bio-sandstone cemented by bio-composite cement.” Construction and Building Materials, 164, 682-687.10.1016/j.conbuildmat.2018.01.014 ↗
52. 52) Zhang, J., Liu, Y., Feng, T., Zhou, M., Zhao, L., Zhou, A. and Li, Z., (2017). “Immobilizing bacteria in expanded perlite for the crack self-healing in concrete.” Construction and Building Materials, 148, 610-617.10.1016/j.conbuildmat.2017.05.021 ↗