Synergistic precipitant powered self-assembly of papain for cross-linked enzyme crystals preparation
Source abstract
Abstract
In this study, we prepared cross-linked enzyme crystals (CLECs) of papain to further broaden the application of the enzyme with high activity in extreme environments. Initially, papain crystals were successfully obtained based on the micro-batch, batch, and expanded batch crystallization experiments. Specifically, ammonium sulfate and polyethylene glycol 6000 (PEG6000) were synergistically used as the precipitants, while l -cysteine was applied to enhance the activity of papain . Furthermore, the interaction between l -cysteine and papain was modeled by molecular docking technique. It was found that l -cysteine could form a hydrogen bond with aspartic acid residue (Asp) at site 158, and the electrostatic attraction with lysine residue (Lys) at site 156 was also quite obvious. Then the enzyme crystals were cross-linked by glutaraldehyde at optimized conditions. The papain CLECs were identified by various methods, and it was found that the thermal stability and enzymatic activity both increased compared to the raw enzyme. More importantly, it could be applied at more rigorous conditions, for example, pH of 4.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Enzyme Catalysis and Immobilization · Enzyme Production and Characterization · Carbohydrate Chemistry and Synthesis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (53)
Show source-provided references
1. Control of the rate of evaporation in protein crystallization by the 'microbatch under oil' method10.1107/S0021889808024667 ↗
2. A novel cross-linked enzyme aggregates (CLEAs) of papain and neutrase-production, partial characterization and application10.1016/j.ijbiomac.2016.11.083 ↗
3. Crystallization of recombinant human growth hormone at elevated pressures: Pressure effects on PEG-induced volume exclusio interactions10.1002/bit.22832 ↗
4. Single disulfide bond reduced papain exists in a compact intermediate state10.1016/S0167-4838(00)00062-5 ↗
5. Studies on Ficin. I. Its isolation and characterization10.1021/bi00841a021 ↗
6. Understanding enzyme immobilisation10.1039/B711564B ↗
7. Protein crystal based materials for nanoscale applications in medicine and biotechnology10.1002/wnan.1547 ↗
8. The interaction of Naphthol Yellow S (NYS) with pepsin: Insights from spectroscopic to molecular dynamics studies10.1016/j.ijbiomac.2020.10.093 ↗
9. Enzymatic hydrolysates of soy protein promote the physicochemical stability of mulberry anthocyanin extracts in food processing10.1016/j.foodchem.2022.132811 ↗
10. Cysteine enhances activity and stability of immobilized papain10.1007/s00726-009-0302-3 ↗
11. Immobilization of laccase via cross-linked enzyme aggregates prepared using genipin as a natural cross-linker10.1016/j.ijbiomac.2020.12.136 ↗
12. Process optimization of preparation of antioxidant chitooligosaccharides by papain
13. Purification and characterization of a proteolytic enzyme from fig latex10.1016/S1005-9040(08)60073-2 ↗
14. Insights on the interaction mechanism of exemestane to three digestive enzymes by multi-spectroscopy and molecular docking10.1016/j.ijbiomac.2021.07.079 ↗
15. Advances and challenges in protein-ligand docking10.3390/ijms11083016 ↗
16. Chelerythrine-lysozyme interaction: Spectroscopic studies, thermodynamics and molecular modeling exploration10.1039/C5CP00424A ↗
17. Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations10.1016/j.ijbiomac.2019.08.246 ↗
18. Protein isoelectric point as a predictor for increased crystallization screening efficiency10.1093/bioinformatics/bth066 ↗
19. Chemically modified papain for applications in detergent formulations10.1016/S0960-8524(00)00178-4 ↗
20. Methods for measuring the concentrations of proteins
21. Optimization of papain treatment for improving the hydrophilicity of polyester fabrics10.1007/s12221-010-0067-z ↗
22. Evaluating enzyme stabilizations in calciumcarbonate: Comparing in situ and crosslinking mediated immobilization10.1016/j.ijbiomac.2021.02.028 ↗
23. Effects of papain concentration, coagulation temperature, and coagulation time on the properties of model soft cheese during ripening
24. Fabricating biopolymer-inorganic hybrid microspheres for enzyme immobilization: Connect membrane emulsification with biomimetic mineralization10.1016/j.partic.2021.07.006 ↗
25. Non-covalent and covalent immobilization of papain onto Ti3C2 MXene nanosheets10.1016/j.enzmictec.2021.109817 ↗
26. Crystallization of Poly (ethylene glycol) in Poly (methyl methacrylate) Networks10.5755/j01.ms.19.2.4430 ↗
27. Structural characteristics of Tilapia (Oreochromis mossambicus) bone gelatin: Effects of different liming methods10.1080/10942912.2014.960929 ↗
28. Novel crystalline catalysts10.1016/0167-7799(96)10031-7 ↗
29. Introduction to protein crystallization10.1107/S2053230X13033141 ↗
30. Glutaraldehyde: Behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking10.2144/04375RV01 ↗
31. Spectroscopic studies on the interaction between chalcone and bovine serum albumin10.1016/j.jlumin.2013.05.013 ↗
32. The novel multi cross-linked enzyme aggregates of protease, lipase, and catalase production from the sunflower seeds, characterization and application10.1016/j.colsurfb.2018.09.042 ↗
33. Cross-linked enzyme crystals (CLECs) of thermolysin in the synthesis of peptides10.1021/ja00115a008 ↗
34. Binding of tolperisone hydrochloride with human serum albumin: Effects on the conformation, thermodynamics, and activity of HSA10.1021/acs.molpharmaceut.7b00976 ↗
35. Cross-linked enzyme aggregates (CLEAs) of Pencilluim notatum lipase enzyme with improved activity, stability and reusability characteristics10.1016/j.ijbiomac.2016.06.081 ↗
36. Preparation and characterization of cross-linked enzyme crystals of laccase10.1016/j.molcatb.2005.11.001 ↗
37. Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs)10.1002/bit.20184 ↗
38. Enhanced activity and stability of papain by covalent immobilization on porous magnetic nanoparticles10.1016/j.ijbiomac.2018.03.088 ↗
39. Imparting functionality to biocatalysts via embedding enzymes into nanoporous materials by a de Novo approach: Size-selective sheltering of catalase in metal–organic framework microcrystals10.1021/ja513058h ↗
40. Cross-linked enzyme crystals of fructose diphosphate aldolase development as a biocatalyst for synthesis10.1016/0040-4039(94)80109-6 ↗
41. Comparison of the sulfur-oxygenation of cysteine and S-carboxymethyl-l-cysteine in human hepatic cytosol and the rôle of cysteine dioxygenase10.1111/jphp.12944 ↗
42. A review: Potential usage of cellulose nanofibers (CNF) forenzyme immobilization via covalent interactions10.1007/s12010-014-1417-x ↗
43. Binding ability of arginine, citrulline, N-acetyl citrulline and thiocitrulline with SARS COV-2 main protease using molecular docking studies
44. In silico and experimental validation of protein-protein interactions between PknI and Rv2159c from Mycobacterium tuberculosis10.1016/j.jmgm.2015.10.011 ↗
45. Molecular docking study of P4-Benzoxaborolesubstituted ligands as inhibitors of HCV NS3/4A protease10.6026/97320630009309 ↗
46. Cross-linking enzyme aggregates in the macropores of silica gel A practical and efficient method for enzyme stabilization10.1016/j.bej.2010.08.003 ↗
47. Exploration of binding of CI Food Red 9 with pepsin by optical spectroscopic and molecular docking methods10.1016/j.saa.2015.05.023 ↗
48. Molecular docking of balanol to dynamics snapshots of protein kinase A10.1002/prot.20688 ↗
49. Chemical crosslinking and the stabilization of proteins and enzymes10.1016/0141-0229(92)90049-T ↗
50. Studies on the interaction mechanism between CXS and papain by spectroscopic and molecular modeling methods10.1007/s11164-012-0682-y ↗
51. Development and workflow of a continuous protein crystallization process: A case of lysozyme10.1021/acs.cgd.8b01534 ↗
52. Properties comparison between free and immobilized wheat esterase using glass fiber film10.1016/j.ijbiomac.2018.12.055 ↗
53. Improved stability of immobilized lipases via modification with polyethylenimine and glutaraldehyde10.1016/j.enzmictec.2017.07.001 ↗