DataVXJournal of Adhesion Science and TechnologyPaper
journal article·2012-09

An ELISA Chip Based on an EWOD Microfluidic Platform

Liang Zhu, Yanying Feng, Xiongying Ye, Jinyang Feng, Yanbin Wu, Zhaoying Zhou

Journal of Adhesion Science and Technology· Volume 26· Issue 12-17
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Source abstract

Abstract

Abstract We have developed an enzyme-linked immunosorbent assay (ELISA) chip based on a digital microfluidic platform, in which droplet generation and manipulation are implemented using the electrowetting on dielectrics (EWOD) effect. First, droplet transportability was experimentally tested on silicon test chips with different control electrodes for optimizing the EWOD-based droplet manipulation, and then tested on transparent indium-tin-oxide (ITO) chips. The latter were then used for the development of an ELISA chip, on which we carried out immunoassay with rat immunoglobulin G (IgG) and goat anti-rat IgG marked with horseradish peroxidase as an example. In driving the reagent solutions, the average velocity of droplets is up to 5–7 mm/s on the ELISA chip, and a chip-scaled immunoassay can be finished within 20 min by using colorimetric detection, with the volume of the sample and reagents of only 0.5–1 μl.
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Research context

OpenAlex topics: Electrowetting and Microfluidic Technologies · Biosensors and Analytical Detection · Microfluidic and Bio-sensing Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Tsinghua University

References (15)

Show source-provided references
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