Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters

Abstract Hybrid nano‐biomaterials are exploited in the design and performance of chemo‐enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) and gold nanoparticles (Au NPs) on magnetic particles coated with silica (MNP@SiO₂) to stepwise hydrolyze and reduce p‐nitrophenyl esters in tandem reaction. The assembly of the two catalysts at the interface of the MNP@SiO₂ particles and the temporal control of the reaction turns out to be the most determinant parameters for the cascade kinetics. When both CALB and Au NPs are co‐immobilized at the MNP@SiO₂ particle, the tandem reactions take place significantly faster than when both catalysts are physically segregated by their immobilization on different MNP@SiO₂ particles. Herein, it is demonstrated that the co‐immobilization of biocatalysts and nanocatalysts in solid materials creates hybrid interfaces that accelerated chemo‐enzymatic tandem reactions.