Sublethal THPS accelerates Pseudomonas-associated steel corrosion by stimulating biofilm development and microbial electron uptake
Source abstract
Abstract
Biocides are widely employed to mitigate microbiologically influenced corrosion (MIC) in oil and gas fields. However, improper biocide dosing may result in changes to the MIC. This study investigated the corrosion behavior of the biofilm-forming Pseudomonas stutzeri on X70 pipeline steel under sublethal concentrations of tetrakis(hydroxymethyl) phosphonium sulfate (THPS) using cell counting, weight loss measurements, surface characterization, and transcriptomic analysis. The results revealed that THPS accelerated the corrosion of X70 steel in the presence of P. stutzeri , compared to THPS-free conditions. While THPS inhibited the growth of planktonic cells, it promoted biofilm formation on the X70 steel surface. Transcriptomic data suggested that this corrosion acceleration could be attributed to metabolic alterations in P. stutzeri , potentially enhancing electron transfer between bacterial cells and the steel surface. These findings provide guidance for the rational application of biocides in industrial settings.
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Research context
OpenAlex topics: Corrosion Behavior and Inhibition · Bacterial biofilms and quorum sensing · Water Treatment and Disinfection
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (62)
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1. Iannuzzi, M. & Frankel, G. The carbon footprint of steel corrosion. Npj. Mater. Degrad. 6, 101 (2022).10.1038/s41529-022-00318-1 ↗
2. Flemming, H. C. Microbial deterioration of materials-fundamentals: economical and technical overview. Mikrobielle Werkstoffzerstoerung-Grundlagen: Oekonomisch-technischer Ueberblick. Werkst. Korros. 45, 1 (1994).10.1002/maco.19940450105 ↗
3. Yazdi, M., Khan, F., Abbassi, R., Quddus, N. & Castaneda-Lopez, H. A review of risk-based decision-making models for microbiologically influenced corrosion (MIC) in offshore pipelines. Reliab. Eng. Syst. Saf. 223, 108474 (2022).10.1016/j.ress.2022.108474 ↗
4. Jia, R., Yang, D., Xu, D. & Gu, T. Anaerobic corrosion of 304 stainless steel caused by the Pseudomonas aeruginosa biofilm. Front. Microbiol. 8, 2335 (2017).10.3389/fmicb.2017.02335 ↗
5. Sudek, L. A., Wanger, G., Templeton, A. S., Staudigel, H. & Tebo, B. M. Submarine basaltic glass colonization by the heterotrophic fe(ii)-oxidizing and siderophore-producing deep-sea bacterium Pseudomonas stutzeri VS-10: the potential role of basalt in enhancing growth Front. Microbiol. 8, 363 (2017).10.3389/fmicb.2017.00363 ↗
6. Zhang, Y. et al. Analysis of marine microbial communities colonizing various metallic materials and rust layers. Biofouling 35, 429–442 (2019).10.1080/08927014.2019.1610881 ↗
7. Lan, G. et al. Corrosion of carbon steel induced by a microbial-enhanced oil recovery bacterium Pseudomonas sp. SWP-4. RSC Adv. 7, 5583–5594 (2017).10.1039/C6RA25154D ↗
8. Abdolahi, A., Hamzah, E., Ibrahim, Z. & Hashim, S. Microbially influenced corrosion of steels by Pseudomonas aeruginosa. Corros. Rev. 32, 129–141 (2014).10.1515/corrrev-2013-0047 ↗
9. Chugh, B. et al. Extracellular electron transfer by Pseudomonas aeruginosa in biocorrosion: a review. ACS Biomater. Sci. Eng. 8, 1049–1059 (2022).10.1021/acsbiomaterials.1c01645 ↗
10. Yuan, S. J., Choong, A. M. F. & Pehkonen, S. O. The influence of the marine aerobic Pseudomonas strain on the corrosion of 70/30 Cu–Ni alloy. Corros. Sci. 49, 4352–4385 (2007).10.1016/j.corsci.2007.04.009 ↗
11. Zhou, E. et al. Accelerated corrosion of 2304 duplex stainless steel by marine Pseudomonas aeruginosa biofilm. Int. Biodeterior. Biodegrad. 127, 1–9 (2018).10.1016/j.ibiod.2017.11.003 ↗
12. Xu, L. J., Ivanova, S. A. & Gu, T. Y. Mitigation of galvanized steel biocorrosion by Pseudomonas aeruginosa biofilm using a biocide enhanced by trehalase. Bioelectrochemistry 154, 108508 (2023).10.1016/j.bioelechem.2023.108508 ↗
13. Wadood, H. Z., Rajasekar, A., Ting, Y. & Sabari, A. N. Role of Bacillus subtilis and Pseudomonas aeruginosa on corrosion behaviour of stainless steel. Arab. J. Sci. Eng. 40, 1825–1836 (2015).10.1007/s13369-015-1590-4 ↗
14. Zhang, S. et al. Corrosion of carbon steel by Pseudomonas stutzeri CQ-Z5 in simulated oilfield water. Bioelectrochemistry 162, 108846 (2025).10.1016/j.bioelechem.2024.108846 ↗
15. Fu, Q. et al. Corrosion mechanism of Pseudomonas stutzeri on X80 steel subjected to Desulfovibrio desulfuricans under elastic stress and yield stress. Corros. Sci. 216, 111084 (2023).10.1016/j.corsci.2023.111084 ↗
16. Liu, H. et al. Characterizations of the biomineralization film caused by marine Pseudomonas stutzeri and its mechanistic effects on X80 pipeline steel corrosion. J. Mater. Sci. Technol. 125, 15–28 (2022).10.1016/j.jmst.2022.02.033 ↗
17. Liu, H. et al. Corrosion inhibition of deposit-covered X80 pipeline steel in seawater containing Pseudomonas stutzeri. Bioelectrochemistry 149, 108279 (2023).10.1016/j.bioelechem.2022.108279 ↗
18. Ramesh, S. & Rajeswari, S. Evaluation of inhibitors and biocide on the corrosion control of copper in neutral aqueous environment. Corros. Sci. 47, 151–169 (2005).10.1016/j.corsci.2004.05.013 ↗
19. Han, Y. et al. Effects of tetrakis (hydroxymethyl) phosphonium sulfate pretreatment on characteristics of sewage sludge. J. Environ. Sci. 78, 174–182 (2019).10.1016/j.jes.2018.09.013 ↗
20. Okoro, C. C., Samuel, O. & Lin, J. The effects of Tetrakis-hydroxymethyl phosphonium sulfate (THPS), nitrite and sodium chloride on methanogenesis and corrosion rates by methanogen populations of corroded pipelines. Corros. Sci. 112, 507–516 (2016).10.1016/j.corsci.2016.08.018 ↗
21. Skovhus, T. L., Eckert, R. B. & Rodrigues, E. Management and control of microbiologically influenced corrosion (MIC) in the oil and gas industry—overview and a North Sea case study. J. Biotechnol. 256, 31–45 (2017).10.1016/j.jbiotec.2017.07.003 ↗
22. Xu, L. et al. Inadequate dosing of THPS treatment increases microbially influenced corrosion of pipeline steel by inducing biofilm growth of Desulfovibrio hontreensis SY-21. Bioelectrochemistry 145, 108048 (2022).10.1016/j.bioelechem.2021.108048 ↗
23. Silva, P. et al. Tetrakis hydroxymethyl phosphonium sulfate (THPS) with biopolymer as strategy for the control of microbiologically influenced corrosion in a dynamic system. Chem. Eng. Process. 160, 108272 (2021).10.1016/j.cep.2020.108272 ↗
24. Xue, Y., Xiao, H. N. & Zhang, Y. Antimicrobial polymeric materials with quaternary ammonium and phosphonium salts. Int. J. Mol. Sci. 16, 3626–3655 (2015).10.3390/ijms16023626 ↗
25. Luo, H. et al. Water quality criteria derivation and tiered ecological risk evaluation of antifouling biocides in marine environment. Mar. Pollut. Bull. 187, 114500 (2023).10.1016/j.marpolbul.2022.114500 ↗
26. Flemming, H. C. et al. Biofilms: an emergent form of bacterial life. Nat. Rev. Microbiol. 14, 563–575 (2016).10.1038/nrmicro.2016.94 ↗
27. Little, B. J., Lee, J. S. & Ray, R. I. The influence of marine biofilms on corrosion: a concise review. Electrochim. Acta 54, 2–7 (2008).10.1016/j.electacta.2008.02.071 ↗
28. Teng, F., Guan, Y. T. & Zhu, W. P. Effect of biofilm on cast iron pipe corrosion in drinking water distribution system: Corrosion scales characterization and microbial community structure investigation. Corros. Sci. 50, 2816–2823 (2008).10.1016/j.corsci.2008.07.008 ↗
29. Mah, T. F. C. & O’Toole, G. A. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 9, 34–39 (2001).10.1016/S0966-842X(00)01913-2 ↗
30. Coenye, T. Response of sessile cells to stress: from changes in gene expression to phenotypic adaptation. FEMS Immunol. Med. Microbiol. 59, 239–252 (2010).10.1111/j.1574-695X.2010.00682.x ↗
31. Turner, A. Marine pollution from antifouling paint particles. Mar. Pollut. Bull. 60, 159–171 (2010).10.1016/j.marpolbul.2009.12.004 ↗
32. Maillard, J. Y. et al. Does microbicide use in consumer products promote antimicrobial resistance? A critical review and recommendations for a cohesive approach to risk assessment. Microb. Drug Resist. 19, 344–354 (2013).10.1089/mdr.2013.0039 ↗
33. Xu, Z. et al. Electrochemical investigation of the “double-edged” effect of low-dose biocide and exogenous electron shuttle on microbial corrosion behavior of carbon steel and copper in enriched seawater. Electrochim. Acta 476, 143687 (2024).10.1016/j.electacta.2023.143687 ↗
34. Gunasekaran, G., Chongdar, S., Gaonkar, S. & Kumar, P. Influence of bacteria on film formation inhibiting corrosion. Corros. Sci. 46, 1953–1967 (2004).10.1016/j.corsci.2003.10.023 ↗
35. Tang, H. et al. Stainless steel corrosion via direct iron-to-microbe electron transfer by geobacter species. ISME J. 15, 3084–3093 (2021).10.1038/s41396-021-00990-2 ↗
36. Cushnie, T. P. T., O’Driscoll, N. H. & Lamb, A. J. Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action. Cell. Mol. Life Sci. 73, 4471–4492 (2016).10.1007/s00018-016-2302-2 ↗
37. Guan, N. et al. Microbial response to environmental stresses: from fundamental mechanisms to practical applications. Appl. Microbiol. Biotechnol. 101, 3991–4008 (2017).10.1007/s00253-017-8264-y ↗
38. Wang, Y. et al. Extracellular polymeric substances and biocorrosion/biofouling: recent advances and future perspectives. Int. J. Mol. Sci. 23, 5566 (2022).10.3390/ijms23105566 ↗
39. Maillard, J.-Y. & Centeleghe, I. How biofilm changes our understanding of cleaning and disinfection. Antimicrob. Resist. Infect. Control. 12, 95 (2023).10.1186/s13756-023-01290-4 ↗
40. Tirandaz, H., Dastgheib, S. M. M. & Hamedi, J. Sub-minimum inhibitory concentration of tetrakis (hydroxymethyl) phosphonium sulfate enhances biocorrosion of carbon steel by Pseudomonas aeruginosa. Sci. Rep. 14, 28918 (2024).10.1038/s41598-024-70157-4 ↗
41. Bas, S., Kramer, M. & Stopar, D. Biofilm surface density determines biocide effectiveness. Front. Microbiol. 8, 2443 (2017).10.3389/fmicb.2017.02443 ↗
42. Hayrapetyan, H., Tempelaars, M., Groot, M. N. & Abee, T. Bacillus cereus ATCC 14579 RpoN (Sigma 54) is a pleiotropic regulator of growth, carbohydrate metabolism, motility, biofilm formation and toxin production. PLoS ONE 10, e0134872 (2015).10.1371/journal.pone.0134872 ↗
43. Hwang, S., Jeon, B., Yun, J. & Ryu, S. Roles of RpoN in the resistance of Campylobacter jejuni under various stress conditions. BMC Microbiol. 11, 1–8 (2011).10.1186/1471-2180-11-207 ↗
44. Dalet, K., Cenatiempo, Y., Cossart, P. & Héchard, Y. A σ54-dependent PTS permease of the mannose family is responsible for sensitivity of Listeria monocytogenes to mesentericin Y105. Microbiology 147, 3263–3269 (2001).10.1099/00221287-147-12-3263 ↗
45. Du, B., Gu, Y., Chen, G., Wang, G. & Liu, L. Flagellar motility mediates early-stage biofilm formation in oligotrophic aquatic environment. Ecotoxicol. Environ. Saf. 194, 110340 (2020).10.1016/j.ecoenv.2020.110340 ↗
46. Zheng, L. et al. Transcriptome sequencing reveals the difference in the expression of biofilm and planktonic cells between two strains of Salmonella Typhimurium. Biofilm 4, 100086 (2022).10.1016/j.bioflm.2022.100086 ↗
47. Guttenplan, S. B. & Kearns, D. B. Regulation of flagellar motility during biofilm formation. FEMS Microbiol. Rev. 37, 849–871 (2013).10.1111/1574-6976.12018 ↗
48. Alonso-Calleja, C., Guerrero-Ramos, E., Alonso-Hernando, A. & Capita, R. Adaptation and cross-adaptation of Escherichia coli ATCC 12806 to several food-grade biocides. Food Control 56, 86–94 (2015).10.1016/j.foodcont.2015.03.012 ↗
49. Elliott, T. & Rodgers, F. Morphological response and growth characteristics of Legionella pneumophila exposed to ampicillin and erythromycin. J. Med. Microbiol. 19, 383–390 (1985).10.1099/00222615-19-3-383 ↗
50. Morente, E. O. et al. Biocide tolerance in bacteria. Int. J. Food Microbiol. 162, 13–25 (2013).10.1016/j.ijfoodmicro.2012.12.028 ↗
51. Yeap, C. S. Y. et al. Dissolved iron released from nanoscale zero-valent iron (nZVI) activates the defense system in bacterium Pseudomonas putida, leading to high tolerance to oxidative stress. J. Hazard. Mater. 439, 129627 (2022).10.1016/j.jhazmat.2022.129627 ↗
52. Fan, W., Qian, C. & Rui, Y. A strong inhibitory effect of microbe-induced mineralization on corrosion on steel surfaces. J. Mater. Eng. Perform. 32, 6957–6973 (2023).10.1007/s11665-022-07586-7 ↗
53. Suma, M. et al. Synergistic action of Bacillus subtilis, Escherichia coli and Shewanella putrefaciens along with Pseudomonas putida on inhibiting mild steel against oxygen corrosion. Appl. Microbiol. Biotechnol. 103, 5891–5905 (2019).10.1007/s00253-019-09866-0 ↗
54. Liu, T. et al. The addition of copper accelerates the corrosion of steel via impeding biomineralized film formation of Bacillus subtilis in seawater. Corros. Sci. 149, 153–163 (2019).10.1016/j.corsci.2019.01.010 ↗
55. Lalucat, J., Bennasar, A., Bosch, R., Garcia-Valdes, E. & Palleroni, N. J. Biology of Pseudomonas stutzeri. Microbiol. Mol. Biol. Rev. 70, 510–547 (2006).10.1128/MMBR.00047-05 ↗
56. Xu, L. et al. Effect of Pseudomonas sp. on the corrosive behavior of metal materials under biocide resistance. Mar. Sci. 44, 14–22 (2020).
57. Wang, D. et al. Distinguishing two different microbiologically influenced corrosion (MIC) mechanisms using an electron mediator and hydrogen evolution detection. Corros. Sci. 177, 108993 (2020).10.1016/j.corsci.2020.108993 ↗
58. Emerson, J. B. et al. Schrödinger’s microbes: tools for distinguishing the living from the dead in microbial ecosystems. Microbiome 5, 1–23 (2017).10.1186/s40168-017-0285-3 ↗
59. Guan, F. et al. Synergistic effect of carbon starvation and exogenous redox mediators on corrosion of X70 pipeline steel induced by Desulfovibrio singaporenus. Sci. Total Environ. 788, 147573 (2021).10.1016/j.scitotenv.2021.147573 ↗
60. GB/T16545-2015. Corrosion of metals and alloys-removal of corrosion products from corrosion test specimen. http://cdn.standards.iteh.ai/samples/59474/84a056036308446f9e39bec39a4ac6c0/ISO-17248-2015.pdf (2015).
61. Lehmann, U. & Kreipe, H. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies. Methods 25, 409–418 (2001).10.1006/meth.2001.1263 ↗
62. Levin, J. Z. et al. Comprehensive comparative analysis of strand-specific RNA sequencing methods. Nat. Meth. 7, 709–715 (2010).10.1038/nmeth.1491 ↗