Isotopes reveal a plant-consuming multi-cultural community in Late Antique Sicily
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Abstract
Abstract Late Antiquity in the Mediterranean region and its border areas marks a period of significant historical change characterised by large-scale population movements and intense cultural interactions that are not yet fully understood. To offer new insights into these local-scale interactions, we employed stable isotope analyses of carbon, nitrogen, and oxygen to reconstruct diet and spatial mobility for 17 individuals from the rural Sicilian sites of Scorrione (Modica) and Cisternazzi (Ragusa). Stable carbon and nitrogen isotope results reveal that most of the analysed individuals relied predominantly on plant protein for their diets. Stable oxygen isotope data indicate that some of the buried individuals likely originated from Central Europe or Northern Africa. By combining the archaeological evidence with scientific analyses, the study contributes to our understanding of the dietary habits and cultural interactions in Sicily during Late Antiquity.
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OpenAlex topics: Archaeology and ancient environmental studies · Cultural Heritage Materials Analysis · Archaeology and Historical Studies
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References (100+)
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1. Cameron, A. The Mediterranean World in Late Antiquity, AD 395-600 (Routledge, 1993).10.4324/9780203329986 ↗
2. Harper, K. The Fate of Rome: Climate, Disease, and the End of an Empire (Princeton Univ. Press, 2017).10.2307/j.ctv9b2txr ↗
3. Wilson, R. J. A. Sicily under the Roman Empire: The Archaeology of a Roman Province, 36 BC-AD 535 (Aris and Phillips, 1990).
4. Castrorao Barba, A. Sicily before the muslims. The transformation of the Roman Villas between Late Antiquity and the Early Middle Ages, fourth to eighth centuries CE. J. Transcult. Mediev. Stud. 3, 145–190 (2016).10.1515/jtms-2016-0005 ↗
5. Caliri, E. Vandals in the Mediterranean: Sicily and its role. In Proc. 15th Symposium on Mediterranean Archaeology 991–995 (BAR International Series 2695, University of Catania, 2015).
6. Chowaniec, R. Vandals, Ostrogoths and the Byzantine footprints in Sicily: an archeological-historical review. Mediterr. Archaeol. Archaeom. 19, 51–61 (2019).
7. Tanasi, D. et al. Diet and mobility in Late Antique Sicily: Isotopic data from the catacombs of Santa Lucia, Siracusa (Italy). J. Archaeol. Sci. Rep. 50, 104096 (2023).
8. Salamon, M. et al. The consilience of historical and isotopic approaches in reconstructing the medieval Mediterranean diet. J. Archaeol. Sci. 35, 1667–1672 (2008).10.1016/j.jas.2007.11.015 ↗
9. Riccomi, G. et al. Stable isotopic reconstruction of dietary changes across Late Antiquity and the Middle Ages in Tuscany. J. Archaeol. Sci. Rep. 33, 102546 (2020).
10. Di Stefano, G. Cava Ispica (Palermo, 1997).
11. Tinner, W. et al. Holocene vegetation and fire history of the mountains of Northern Sicily (Italy). Veg. Hist. Archaeobotany 25, 499–519 (2016).10.1007/s00334-016-0569-8 ↗
12. Baldi, M. et al. Remote climate processes underlying summer drought events in the Mediterranean. In Balwois Conference 2004 1–12 (Ohrid, 2004).
13. Burgio, A., Cugno, S. A. & Ricciardi, T. I sepolcri rupestri a baldacchino di Siracusa [The rock-cut baldachyn tombs of Syracuse]. in Archeologia rupestre nel territorio di Siracusa 79–120 (Oxford, 2020).
14. Rizzone, V. & Sammito, A. M. Modica e Il Suo Territorio Nella Tarda Antichità [Modica and Its Territory in Late Antiquity] (Modica, 2001).
15. Rizzone, V. & Sammito, A. M. Gli ipogei funerari di tipo comunitario nell’altopiano di Ragusa [Community-Type Funerary Hypogea on the Ragusa Plateau]. in Gestione integrata e promozione dei siti archeologici a Ragusa e Malta, I siti archeologici del periodo tardoantico (Palermo, 2013).
16. Rizzone, V. & Sammito, A. M. Sepolture di prestigio nelle catacombe della Sicilia sud-orientale e di Malta [Prestigious Burials in the Catacombs of Southeastern Sicily and Malta]. in Sepolture di prestigio nel bacino mediterraneo (IV-IX ss.): definizione, immagini, utilizzo 341–354 (Florence, 2021).
17. Vitale, E. Ricerche Nella Catacomba Di Villagrazia Di Carini [Research in the Catacomb of Villagrazia di Carini]. (Palermo, 2018).
18. Hukeľová, Z., Schmidtová, D., Pinar Gil, J. & Scerra, S. Discovering the catacombs of Sicily. First analysis of the skeletal remains from the Late Antique Hypogeum of Scorrione. Študijné Zvesti Archeol. Úst. SAV 70, 391–402 (2023).
19. Cangialosi, E. O., Lauria, G., Toscano Raffa, A. & Meli, F. Analisi antropologica dei reperti osteologici tardo-antichi provenienti dal contesto ipogeico di Licata. L’ipogeo Zirafi-settore G ‒ Licata (Sicilia) [Anthropological Analysis of Late Antique Osteological Remains from the Hypogeal Context of Licata: the Zirafi Hypogeum – Sector G, Licata (Sicily)]. Arch. Antropol. E Etnologia 152, 61–74 (2022).10.36253/aae-2361 ↗
20. Hedges, R. E. M. & Reynard, L. M. Nitrogen isotopes and the trophic level of humans in archaeology. J. Archaeol. Sci. 34, 1240–1251 (2007).10.1016/j.jas.2006.10.015 ↗
21. Fernandes, R., Nadeau, M.-J. & Grootes, P. M. Macronutrient-based model for dietary carbon routing in bone collagen and bioapatite. Archaeol. Anthropol. Sci. 4, 291–301 (2012).10.1007/s12520-012-0102-7 ↗
22. Ambrose, S. H. & Norr, L. in Prehistoric Human Bone (eds Lambert, J. B. & Grupe, G.) (Springer, 1993).
23. DeNiro, M. J. & Epstein, S. Influence of diet on the distribution of carbon isotopes in animals. Geochim. Cosmochim. Acta 42, 495–506 (1978).10.1016/0016-7037(78)90199-0 ↗
24. Tykot, R. H. in Histories of Maize (New York, 2006).
25. Stevens, R. E., Pederzani, S., Britton, K. & Wexler, S. K. Bones and teeth isotopes as archives for palaeoclimatic, palaeoenvironmental and palaeoecological data. Quat. Sci. Rev. 357, 109320 (2025).10.1016/j.quascirev.2025.109320 ↗
26. Tykot, R. H. Stable isotopes and diet: you are what you eat. In Proc. International School of Physics “Enrico Fermi” Course CLIV 433–444 (IOS Press, 2004).
27. Depaermentier, M. L. C. et al. The palaeoenvironmental potential of bioarchaeological isotope data. Commun. Earth Environ. 6, 501 (2025).10.1038/s43247-025-02507-7 ↗
28. Kohn, M. J. & Cerling, T. E. Stable isotope compositions of biological apatite. Rev. Mineral. Geochem. 48, 455–488 (2002).10.2138/rmg.2002.48.12 ↗
29. Schoeninger, M. J. & Moore, K. Bone stable isotope studies in archaeology. J. World Prehistory 6, 247–296 (1992).10.1007/BF00975551 ↗
30. Bogaard, A., Heaton, T. H. E., Poulton, P. & Merbach, I. The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices. J. Archaeol. Sci. 34, 335–343 (2007).10.1016/j.jas.2006.04.009 ↗
31. Fraser, R. A. et al. Manuring and stable nitrogen isotope ratios in cereals and pulses: towards a new archaeobotanical approach to the inference of land use and dietary practices. J. Archaeol. Sci. 38, 2790–2804 (2011).10.1016/j.jas.2011.06.024 ↗
32. Szpak, P. Complexities of nitrogen isotope biogeochemistry in plant-soil systems: implications for the study of ancient agricultural and animal management practices. Front. Plant Sci. 5, 288 (2014).10.3389/fpls.2014.00288 ↗
33. O’Connell, T. C., Kneale, C. J., Tasevska, N. & Kuhnle, G. G. C. The diet-body offset in human nitrogen isotopic values: a controlled dietary study. Am. J. Phys. Anthropol. 149, 426–434 (2012).10.1002/ajpa.22140 ↗
34. Caut, S., Angulo, E. & Courchamp, F. Variation in discrimination factors (Δ15 N and Δ13 C): the effect of diet isotopic values and applications for diet reconstruction. J. Appl. Ecol. 46, 443–453 (2009).10.1111/j.1365-2664.2009.01620.x ↗
35. Hedges, R., Rush, E. & Aalbersberg, W. Correspondence between human diet, body composition and stable isotopic composition of hair and breath in Fijian villagers. Isotopes Environ. Health Stud. 45, 1–17 (2009).10.1080/10256010802522010 ↗
36. Pederzani, S. & Britton, K. Oxygen isotopes in bioarchaeology: principles and applications, challenges and opportunities. Earth Sci. Rev. 188, 77–107 (2019).10.1016/j.earscirev.2018.11.005 ↗
37. Lightfoot, E. & O’Connell, T. C. On the use of biomineral oxygen isotope data to identify human migrants in the archaeological record: intra-sample variation, statistical methods and geographical considerations. PLoS ONE 11, e0153850 (2016).10.1371/journal.pone.0153850 ↗
38. Dotsika, E. Correlation between δ18Ow and δ18Οen for estimating human mobility and paleomobility patterns. Sci. Rep. 10, 15439 (2020).10.1038/s41598-020-71683-7 ↗
39. Daux, V. et al. Oxygen isotope fractionation between human phosphate and water revisited. J. Hum. Evol. 55, 1138–1147 (2008).10.1016/j.jhevol.2008.06.006 ↗
40. Royer, A., Daux, V., Fourel, F. & Lécuyer, C. Carbon, nitrogen and oxygen isotope fractionation during food cooking: implications for the interpretation of the fossil human record. Am. J. Phys. Anthropol. 163, 759–771 (2017).10.1002/ajpa.23246 ↗
41. Lisowska-Gaczorek, A., Szostek, K., Pawlyta, J. & Cienkosz-Stepańczak, B. Oxygen isotopic fractionation in rat bones as a result of consuming thermally processed water – bioarchaeological applications. Geochronometria 47, 1–12 (2020).10.2478/geochr-2020-0001 ↗
42. Lisowska-Gaczorek, A., Cienkosz-Stepańczak, B., Furmanek, M. & Szostek, K. A new perspectives on breastfeeding practice reconstruction in bioarchaeology – an oxygen isotopes study in an animal model. Anthropol. Rev. 86, 129–159 (2023).10.18778/1898-6773.86.3.08 ↗
43. Scheid, R. C. & Woelfel, J. B. Woelfel’s Dental Anatomy (Wolters Kluwer/Lippincott Williams & Wilkins Health, 2012).
44. Cocozza, C. & Fernandes, R. Tooth formation age dataset for early childhood bioarchaeological and medical studies. Data Brief 36, 107141 (2021).10.1016/j.dib.2021.107141 ↗
45. Wilson, R. J. A. Dining with the Dead in Early Byzantine Sicily: Excavations at Punta Secca near Ragusa (The BABESCH Foundation, 2017).
46. Fiorentino, G., Grasso, A. M. & Primavera, M. Introduction, spread and selective breeding of crops: new archaeobotanical data from southern Italy in the early Middle Ages. Veg. Hist. Archaeobotany https://doi.org/10.1007/s00334-024-00989-7 (2024).10.1007/s00334-024-00989-7 ↗
47. Stika, H.-P., Heiss, A. G. & Zach, B. Plant remains from the early Iron Age in western Sicily: differences in subsistence strategies of Greek and Elymian sites. Veg. Hist. Archaeobotany 17, 139–148 (2008).10.1007/s00334-008-0171-9 ↗
48. Reitsema, L. J., Kyle, B. & Vassallo, S. Food traditions and colonial interactions in the ancient Mediterranean: Stable isotope evidence from the Greek Sicilian colony Himera. J. Anthropol. Archaeol. 57, 101144 (2020).10.1016/j.jaa.2020.101144 ↗
49. Ramsay, J. & Wilson, R. J. A. Funerary dining in early byzantine Sicily: Archaeobotanical evidence from Kaukana. Mediterr. Archaeol. 26, 81–93 (2013).
50. Chowaniec, R., Dotsika, E. & Grezak, A. Late Hellenistic to Later Roman/Byzantine periods faunal and flora assemblage in the ancient Akrai (south–eastern Sicily). Environment and food circulation reconstruction. In On the Borders of Syracuse: Multidisciplinary Studies on the Ancient Town of Akrai/Acrae, (ed. Chowaniec, S. R.), Warsaw 2018, 145–171 (Instytut Archeologii UW, 2018).
51. Carra, M. et al. Plant foods in the Late Palaeolithic of Southern Italy and Sicily: integrating carpological and dental calculus evidence. Quat. Int. 653–654, 53–68 (2023).10.1016/j.quaint.2022.06.007 ↗
52. Tanasi, D., Tykot, R. H., Vianello, A. & Hassam, S. Stable isotope analysis of the dietary habits of a Greek community in Archaic Syracuse (Sicily): a pilot study. STAR Sci. Technol. Archaeol. Res. 3, 466–477 (2017).
53. Vaccaro, E., MacKinnon, M. & Mercuri, A. M. in Humans and Environmental Sustainability (eds Florenzano, A., Montecchi, M. C. & Rinaldi, R.) (Modena, 2018).
54. Aniceti, V. Animals and their roles in the medieval society of Sicily: from Byzantines to Arabs and from Arabs to Norman/Swabians. (University of Sheffield, 2019).
55. Purcell, N. in Food in Antiquity (Exeter, 1995).
56. Monnereau, A. et al. Multi-proxy bioarchaeological analysis of skeletal remains shows genetic discontinuity in a Medieval Sicilian community. R. Soc. Open Sci. 11, 240436 (2024).10.1098/rsos.240436 ↗
57. Bruno, O., Scerra, S. & Sirugo, S. L’ipogeo di Cisternazzi: lo scavo e i rinvenimenti archeologici [The Cisternazzi Hypogeum: Excavation and Archaeological Findings]. In Gestione integrata e promozione dei siti archeologici a Ragusa e Malta, I siti archeologici del periodo tardoantico 101–109 (Palermo, 2013).
58. Longin, R. New Method of Collagen Extraction for Radiocarbon Dating. Nature 230, 241–242 (1971).10.1038/230241a0 ↗
59. Ventresca Miller, A. et al. Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis. J. Vis. Exp. 58002 (2018) https://doi.org/10.3791/58002.10.3791/58002 ↗
60. Coplen, T. B. Normalization of oxygen and hydrogen isotope data. Chem. Geol. Isot. Geosci. Sect. 72, 293–297 (1988).10.1016/0168-9622(88)90042-5 ↗
61. Cocozza, C. et al. A Bayesian multi-proxy contribution to the socioeconomic, political, and cultural history of late medieval Capitanata (southern Italy). Sci. Rep. 13, 4078 (2023).10.1038/s41598-023-30706-9 ↗
62. Chenery, C. A., Pashley, V., Lamb, A. L., Sloane, H. J. & Evans, J. A. The oxygen isotope relationship between the phosphate and structural carbonate fractions of human bioapatite. Rapid Commun. Mass Spectrom. 26, 309–319 (2012).10.1002/rcm.5331 ↗
63. Pollard, A. M., Pellegrini, M. & Lee-Thorp, J. A. Technical note: Some observations on the conversion of dental enamel δ18 op values to δ18 ow to determine human mobility. Am. J. Phys. Anthropol. 145, 499–504 (2011).10.1002/ajpa.21524 ↗
64. Velte, M. et al. Tracing early life histories from Roman times to the Medieval era: weaning practices and physiological stress. Archaeol. Anthropol. Sci. 15, 190 (2023).10.1007/s12520-023-01882-6 ↗
65. Chinique De Armas, Y., Mavridou, A.-M., Garcell Domínguez, J., Hanson, K. & Laffoon, J. Tracking breastfeeding and weaning practices in ancient populations by combining carbon, nitrogen and oxygen stable isotopes from multiple non-adult tissues. PLoS ONE 17, e0262435 (2022).10.1371/journal.pone.0262435 ↗
66. Tsutaya, T. & Yoneda, M. Quantitative reconstruction of weaning ages in archaeological human populations using bone collagen nitrogen isotope ratios and approximate Bayesian computation. PLoS ONE 8, e72327 (2013).10.1371/journal.pone.0072327 ↗
67. Roberts, S. B. et al. Effect of weaning on accuracy of doubly labeled water method in infants. Am. J. Physiol. Regul. Integr. Comp. Physiol. 254, R622–R627 (1988).10.1152/ajpregu.1988.254.4.R622 ↗
68. Steinhof, A. Data analysis at the Jena14 C laboratory. Radiocarbon 55, 282–293 (2013).10.1017/S0033822200057386 ↗
69. Steinhof, A., Altenburg, M. & Machts, H. Sample preparation at the Jena14 C laboratory. Radiocarbon 59, 815–830 (2017).10.1017/RDC.2017.50 ↗
70. Wacker, L. et al. MICADAS: routine and high-precision radiocarbon dating. Radiocarbon 52, 252–262 (2010).10.1017/S0033822200045288 ↗
71. R Core Team. R: a language and environment for statistical computing. R Foundation for Statistical Computing (2021).
72. Terzer, S., Wassenaar, L. I., Araguás-Araguás, L. J. & Aggarwal, P. K. Global isoscapes for δ18 O and δ2 H in precipitation: improved prediction using regionalized climatic regression models. Hydrol. Earth Syst. Sci. 17, 4713–4728 (2013).10.5194/hess-17-4713-2013 ↗
73. Cubas, M. et al. Latitudinal gradient in dairy production with the introduction of farming in Atlantic Europe. Nat. Commun. 11, 2036 (2020).10.1038/s41467-020-15907-4 ↗
74. Fernandes, R., Millard, A. R., Brabec, M., Nadeau, M.-J. & Grootes, P. Food reconstruction using isotopic transferred signals (FRUITS): a Bayesian model for diet reconstruction. PLoS ONE 9, e87436 (2014).10.1371/journal.pone.0087436 ↗
75. Soncin, S. et al. High-resolution dietary reconstruction of victims of the 79 CE Vesuvius eruption at Herculaneum by compound-specific isotope analysis. Sci. Adv. 7, eabg5791 (2021).10.1126/sciadv.abg5791 ↗
76. Fernandes, R., Grootes, P., Nadeau, M. & Nehlich, O. Quantitative diet reconstruction of a Neolithic population using a Bayesian mixing model (FRUITS): The case study of Ostorf (Germany). Am. J. Phys. Anthropol. 158, 325–340 (2015).10.1002/ajpa.22788 ↗
77. Barbara, W. Archeozoologia. Manuale per Lo Studio Dei Resti Faunistici Dell’area Mediterranea [A Manual for the Study of Faunal Remains from the Mediterranean Area] (2002).
78. Hillson, S. Mammal Bones and Teeth: An Introductory Guide to Methods of Identification (Taylor & Francis, 2016).10.4324/9781315425016 ↗
79. Hillson, S. Teeth (Cambridge Univ. Press, 2005).10.1017/CBO9780511614477 ↗
80. Brown, S. et al. Zooarchaeology by mass spectrometry (ZooMS)- Pretreatment protocols for bone material v1. https://doi.org/10.17504/protocols.io.bf5bjq2n (2020).10.17504/protocols.io.bf5bjq2n ↗
81. Buckley, M., Collins, M., Thomas-Oates, J. & Wilson, J. C. Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 23, 3843–3854 (2009).10.1002/rcm.4316 ↗
82. Welker, F., Soressi, M., Rendu, W., Hublin, J.-J. & Collins, M. Using ZooMS to identify fragmentary bone from the Late Middle/Early Upper Palaeolithic sequence of Les Cottés, France. J. Archaeol. Sci. 54, 279–286 (2015).10.1016/j.jas.2014.12.010 ↗
83. Strohalm, M., Kavan, D., Novák, P., Volný, M. & Havlíček, V. mMass 3: a cross-platform software environment for precise analysis of mass spectrometric data. Anal. Chem. 82, 4648–4651 (2010).10.1021/ac100818g ↗
84. Van Klinken, G. J. Bone collagen quality indicators for palaeodietary and radiocarbon measurements. J. Archaeol. Sci. 26, 687–695 (1999).10.1006/jasc.1998.0385 ↗
85. Pinar Gil, J. & Pleska, M. Two unusual funerary deposits from the migration period at the Hypogeum D, Modica-Scorrione W (Prov. Ragusa, Siciliy / I). Archäol. Korresp. 52, 545–562 (2023).
86. Pinar Gil, J. et al. in Hyblaea: studi di archeologia e topografia dell’altopiano ibleo (Archaeopress Publishing Limited, 2022).
87. Pinar Gil, J. & Pleska, M. Mogil’nik s peščernymi sklepami v Modike-Scorrione Zapad (Ragusa, Italia) i period velikogo pereselenia narodov na Sicilii [A burial ground with rock-cut tombs in Modica–Scorrione West (Ragusa, Italy) and the period of the Great Migration on Sicily]. Kratk. Soobščenia Instituta Archeol. 268, 7–18 (2022).
88. Ambrose, S. H. Preparation and characterization of bone and tooth collagen for isotopic analysis. J. Archaeol. Sci. 17, 431–451 (1990).10.1016/0305-4403(90)90007-R ↗
89. Wallace, M. et al. Stable carbon isotope analysis as a direct means of inferring crop water status and water management practices. World Archaeol 45, 388–409 (2013).10.1080/00438243.2013.821671 ↗
90. Kellner, C. M. & Schoeninger, M. J. A simple carbon isotope model for reconstructing prehistoric human diet. Am. J. Phys. Anthropol. 133, 1112–1127 (2007).10.1002/ajpa.20618 ↗
91. Carver, M. et al. Sicily in transition. New research on early medieval Sicily, 2017-2018. J. Fasti Online https://www.fastionline.org/s/folder/item/97074 (2019).
92. Collins, R. P. & Jones, M. B. The influence of climatic factors on the distribution of C4 species in Europe. Vegetatio 64, 121–129 (1986).10.1007/BF00044788 ↗
93. Piazza, A., Conti, F., Viola, F., Eccel, E. & Noto, L. Comparative analysis of spatial interpolation methods in the Mediterranean area: application to temperature in Sicily. Water 7, 1866–1888 (2015).10.3390/w7051866 ↗
94. Bowes, K. (ed.) The Roman Peasant Project 2009-2014: Excavating the Roman Rural Poor. (University of Pennsylvania Museum, 2021).10.9783/9781949057089 ↗
95. Varano, S. et al. The edge of the Empire: diet characterization of medieval Rome through stable isotope analysis. Archaeol. Anthropol. Sci. 12, 196 (2020).10.1007/s12520-020-01158-3 ↗
96. Cocozza, C., Cirelli, E., Groß, M., Teegen, W.-R. & Fernandes, R. Presenting the compendium isotoporum Medii Aevi, a multi-isotope database for Medieval Europe. Sci. Data 9, 354 (2022).10.1038/s41597-022-01462-8 ↗
97. Cummings, C. Meat consumption in Roman Britain: the evidence from stable isotopes. Theor. Roman Archaeol. J. 0, 73 (2009).10.16995/TRAC2008_73_83 ↗
98. Garnsey, P. Food and Society in Classical Antiquity (Cambridge Univ. Press, 1999).10.1017/CBO9780511612534 ↗
99. Donahue, J. The Roman Community at Table during the Principate (University of Michigan Press, 2004).10.3998/mpub.22968 ↗
100. Wilkins, J. & Hill, S. Food in the Ancient World (John Wiley & Sons, 2009).10.1002/9781444308372.ch30 ↗