journal article·2018-07-26

Improving the efficiency of enzymatic hydrolysis of Eucalyptus residues with a modified aqueous ammonia soaking method

Dan Huo, Qiulin Yang, Guigan Fang, Qiujuan Liu, Chuanling Si, Qingxi Hou, Bin Li

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Abstract

Abstract Eucalyptus residues from pulp mill were pretreated with aqueous ammonia soaking (AAS) method to improve the efficiency of enzymatic hydrolysis. The optimized condition of AAS was obtained by response surface methodology. Meanwhile, hydrogen peroxide was introduced into the AAS system to modify the AAS pretreatment (AASP). The results showed that a fermentable sugar yield of 64.96 % was obtained when the eucalypt fibers were pretreated at the optimal conditions, with 80 % of ammonia (w/w) for 11 h and keeping the temperature at 90 °C. In further research it was found that the addition of H2O2 to the AAS could improve the pretreatment efficiency. The delignification rate and enzymatic digestibility were increased to 64.49 % and 73.85 %, respectively, with 5 % of hydrogen peroxide being used. FTIR analysis indicated that most syringyl and guaiacyl lignin and a trace amount of xylan were degraded and dissolved during the AAS and AASP pretreatments. The CrI of the raw material was increased after AAS and AASP pretreatments, which was attributed to the removal of amorphous portion. SEM images showed that microfibers were separated and explored from the initial fiber structure after AAS pretreatment, and the AASP method could improve the destructiveness of the fiber surface.
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Research context

OpenAlex topics: Biofuel production and bioconversion · Advanced Cellulose Research Studies · biodegradable polymer synthesis and properties

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Tianjin Key Laboratory of Pulp and Paper , College of Papermaking Science and Technology , Tianjin University of Science and Technology , Tianjin 300457 , China

Key Laboratory of Renewable Energy , Chinese Academy of Sciences , Guangzhou 510640 , China

State Key Laboratory of Pulp and Paper Engineering , South China University of Technology , Guangzhou , China , 510640

Jiangsu Province Biomass Energy and Materials Laboratory , Institute of Chemical Industry of Forest Products , CAF , Nanjing 210042 , China

Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals , Nanjing Forestry University , Nanjing 210042 , China

Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province , Zhejiang University of Science and Technology , Hangzhou 310023 , China

Institute of Chemical Industry of Forest Products , Chinese Academy of Forestry , Nanjing 210042 , Jiangsu , China

CAS Key Laboratory of Bio-based Materials , Qingdao Institute of Bioenergy and Bioprocess Technology , Chinese Academy of Sciences , Qingdao 266101 , China

Funding

Chinese Academy of Sciences · South China University of Technology · Nanjing Forestry University · Chinese Academy of Forestry · Zhejiang University of Science and Technology · Tianjin University of Science and Technology

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