Advanced bioprinting strategies for fabrication of biomimetic tissues and organs

Abstract Bioprinting is a revolutionary technology within the field of tissue engineering that enables the precise fabrication of three-dimensional (3D) tissue constructs. It combines the principles of engineering and biology to create structures that closely mimic the complexity of native human tissues, facilitating advancements in regenerative medicine and personalized healthcare. This review paper systematically explores the challenges and design requirements in the fabrication of 3D biomimetic tissue constructs, emphasizing the need for advanced bioprinting strategies. Achieving biomimicry involves creating 3D anatomically relevant structures, biomimetic microenvironments, and vascularization. The focus is on overcoming existing bottlenecks through advancements in both fabrication techniques and bio-inks. Future directions in bioprinting are outlined, including multi-modal bioprinting systems, in-situ bioprinting, and the integration of machine learning into bioprinting processes. The critical role of bio-inks and printing methodologies in influencing cell viability is highlighted, providing insights into strategies for enhancing cellular functionality throughout the bioprinting process. Furthermore, the paper addresses post-fabrication considerations, particularly in accelerating tissue maturation, as a pivotal component for advancing the clinical applicability of bioprinted tissues. By navigating through the challenges, innovations, and prospects of advanced bioprinting strategies, this review highlights the transformative impact on tissue engineering. Pushing the boundaries of technological capabilities, these strategies hold the promise of groundbreaking advancements in regenerative medicine and personalized healthcare. Ultimately, the integration of these advanced techniques into bioprinting processes will pave the way for the development of more highly biomimetic and functional bioprinted tissues.