Novel antibacterial Cu/Mg‐substituted 58S‐bioglass: Synthesis, characterization and investigation of in vitro bioactivity
Source abstract
Abstract
Abstract In this study, six sol‐gel copper/magnesium substituted derivatives of 58S‐BG, that is, a mol% series of 60SiO 2 –4P 2 O 5 –5CuO–(31–x) CaO/xMgO (where x = 0, 1, 3, 5, 8, and 10), were synthesized as new multifunctional bioactive glasses (BGs). Afterwards, the effect of MgO/CaO substitution on the in vitro formation of nanohydroxyapatite (HA), osteoblast‐like cell responses, and bioactive glasses antibacterial performance were studied. X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) results revealed that Cu‐substituted 58S‐BG consisting of 5 mol% MgO (BG‐5/5) had capability of the formation of HA on its surface while, Cu‐substituted 58S‐BGs consisting 8 mol% and 10 mol% MgO (BG‐5/8 and BG‐5/10) displayed lower bioactivity. The In vitro investigations proved that the highest values of both differentiation and proliferation of MC3T3‐E1 cells can be obtained from a 5 mol% MgO substituted BG. Furthermore, these novel Cu/Mg‐substituted 58S‐BGs displayed antibacterial effect against methicillin‐resistant staphylococcus aureus bacteria. Taken together, the results suggest the equally substituted BG‐5/5 ( ie., the one consists of 5 mol% of both CuO and MgO) as a promising candidate for bone tissue engineering, among all newly designed BGs in this work, owing to its desirable cell proliferation, ALP activity, and antibacterial properties.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Facial Trauma and Fracture Management · Dental Implant Techniques and Outcomes
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Materials Science and Engineering Faculty of Technology and Engineering Imam Khomeini International University Qazvin Iran
Department of Materials Science and Engineering K. N. Toosi University of Technology Tehran Iran
Department of Molecular Sciences Macquarie University North Ryde NSW Australia
Department of Mechanical Engineering Amirkabir University of Technology Tehran Iran
Department of Material Science and Engineering, Science and Research Branch Islamic Azad University Tehran Iran
Young Researchers and Elite Club Najafabad Branch Islamic Azad University Najafabad Iran
Marquette University School of Dentistry Milwaukee WI USA
References (84)
Show source-provided references
1. Thirty‐five years of guided tissue engineering10.1016/j.jnoncrysol.2007.06.100 ↗
2. Orthopaedic tissue engineering and bone regeneration10.3233/THC-2007-15106 ↗
3. Progress in orthopedic biomaterials and drug delivery10.1007/s13346-016-0288-9 ↗
4. The experimental and numerical study of fracture behavior of 58s bioactive glass/polysulfone composite using the extended finite elements method10.1088/2053-1591/ab3495 ↗
5. Microstructure, strength and CO2 separation characteristics of α‐alumina supported γ‐alumina thin film membrane10.1179/1743676112Y.0000000043 ↗
6. The relationship between the synthesis and morphology of SnO2‐Ag2O nanocomposite10.1080/15533174.2013.783862 ↗
7. Bioactivity and osteoinductivity of glasses and glassceramics and their material determinants10.1016/j.ceramint.2016.06.077 ↗
8. The story of bioglass
9. Characterization of melt‐derived 45S5 and sol‐gel‐derived 58S bioactive glasses10.1002/jbm.10026 ↗
10. A new quantitative method to evaluate the in vitro bioactivity of melt and sol‐gel‐derived silicate glasses10.1002/jbm.a.10503 ↗
11. Synthesis, characterization and bioactivity investigation of bioglass/hydroxyapatite composite10.1016/j.ceramint.2009.09.016 ↗
12. Formation of hydroxyapatite on surface of SiO2–P2O5–CaO–SrO–ZnO bioactive glass synthesized through sol‐gel route10.1016/j.ceramint.2019.06.183 ↗
13. Synthesis and characterization of natural Nano‐hydroxyapatite derived from Turkey Femur‐Bone waste10.1007/s12010-019-03046-6 ↗
14. Recent evidence on bioactive glass antimicrobial and antibiofilm activity: a Mini‐Review10.3390/ma11020326 ↗
15. Synthesis and characteristics of sol‐gel bioactive SiO2‐P2O5‐CaO‐Ag2O glasses10.1016/j.jnoncrysol.2017.09.035 ↗
16. A comparative investigation on bioactivity and antibacterial properties of Sol‐Gel derived 58S bioactive glass substituted by Ag and Zn
17. Synthesis and in vitro studies of sol‐gel derived lithium substituted 58S bioactive glass10.1016/j.ceramint.2017.06.174 ↗
18. Characterization, in vitro bioactivity and biological studies of sol‐gel synthesized SrO substituted 58S bioactive glass10.1016/j.ceramint.2017.08.004 ↗
19. Effect of MgO addition on the crystallization and in vitro bioactivity of glass ceramics in the CaO–MgO–SiO2–P2O5 system10.1016/j.ceramint.2012.05.056 ↗
20. The effect of magnesium content on in vitro bioactivity, biological behavior and antibacterial activity of sol–gel derived 58S bioactive glass10.1016/j.ceramint.2018.02.159 ↗
21. Synthesis, characterization and microbiological response of silver doped bioactive glass nanoparticles10.1016/j.ceramint.2011.05.158 ↗
22. Copper‐containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity10.1016/j.biomaterials.2012.09.066 ↗
23. Characterization and in vitro bioactivity of zinc‐containing bioactive glass and glass‐ceramics10.1177/0885328206054535 ↗
24. Unanticipated stabilization of zinc‐silicate glasses by addition of lanthanum: Implications for therapeutic inorganic ion delivery systems10.1016/j.jnoncrysol.2015.08.028 ↗
25. Enhanced antimicrobial properties of bioactive glass using strontium and silver oxide nanocomposites10.1080/21870764.2018.1564477 ↗
26. Synthesis, characterization and in vitro bioactivity of magnesium‐doped sol–gel glass and glass‐ceramics10.1016/j.ceramint.2011.01.043 ↗
27. In vitro reactivity of Sr‐containing bioactive glass (type 1393) nanoparticles10.1016/j.jnoncrysol.2013.12.010 ↗
28. Bioactive glasses: special applications outside the skeletal system10.1016/j.jnoncrysol.2015.02.015 ↗
29. Magnesium chemistry and biochemistry10.1023/A:1016058229972 ↗
30. Genetic causes of hypomagnesemia, a clinical overview10.1007/s00467-016-3416-3 ↗
31. Magnesium and osteoporosis: current state of knowledge and future research directions10.3390/nu5083022 ↗
32. Magnesium deficiency results in an increased formation of osteoclasts10.1016/j.jnutbio.2012.12.008 ↗
33. Effect of dietary magnesium deficiency on the bones and teeth of rats10.1177/00220345650440063401 ↗
34. Functional role of inorganic trace elements in angiogenesis—Part II: Cr, Si, Zn, Cu, and S10.1016/j.critrevonc.2015.05.011 ↗
35. Role of copper in tumour angiogenesis—clinical implications10.1016/j.jtemb.2004.02.004 ↗
36. Antibacterial activity of silicate bioceramics10.1007/s11595-011-0202-8 ↗
37. Bioactive and antibacterial glass powders doped with copper by ion‐exchange in aqueous solutions10.3390/ma9060405 ↗
38. Copper‐containing mesoporous bioactive glass nanoparticles as multifunctional agent for bone regeneration10.1016/j.actbio.2017.04.012 ↗
39. Sol–gel synthesis and in vitro bioactivity of copper and zinc‐doped silicate bioactive glasses and glass‐ceramics10.1088/1748-6041/10/2/025001 ↗
40. Preparation of copper‐containing bioactive glass/eggshell membrane nanocomposites for improving angiogenesis, antibacterial activity and wound healing10.1016/j.actbio.2016.03.011 ↗
41. Copper‐containing mesoporous bioactive glass coatings on orbital implants for improving drug delivery capacity and antibacterial activity10.1007/s10529-014-1465-x ↗
42. Preliminary investigation of the effect of doping of copper oxide in CaO‐SiO2‐P2O5‐MgO bioactive composition for bone repair applications10.1016/j.msec.2017.09.006 ↗
43. Surface and chemical study of SiO2·P2O5·CaO·(MgO) bioactive glasses10.1021/cm9911114 ↗
44. Effect of magnesia on the degradability and bioactivity of sol–gel derived SiO2–CaO–MgO–P2O5 system glasses10.1016/j.colsurfb.2010.06.022 ↗
45. Effect of magnesium content on the in vitro bioactivity of CaO‐MgO‐SiO2‐P2O5 sol‐gel glasses10.1039/a808679f ↗
46. In vitro bioactivity of melt‐derived glass 46S6 doped with magnesium
47. Role of magnesium oxide and strontium oxide as modifiers in silicate‐based bioactive glasses: Effects on thermal behaviour, mechanical properties and in‐vitro bioactivity10.1016/j.msec.2016.11.110 ↗
48. Influence of magnesia on the structure and properties of bioactive glasses10.1016/j.jnoncrysol.2009.04.074 ↗
49. Synthesis, characterization and biological response of magnesium‐substituted nanobioactive glass particles for biomedical applications10.1016/j.ceramint.2012.08.011 ↗
50. Synthesis, characterization, and In vitro bioactivity of Sol‐Gel‐Derived Zn, Mg, and Zn‐Mg Co‐doped bioactive glasses10.1002/ceat.200900495 ↗
51. In vitro bioactivity of glass and glass‐ceramics of the 3CaO·P2O5–CaO·SiO2–CaO·MgO·2SiO2 system10.1016/S0142-9612(99)00150-7 ↗
52. Synthesis, characterization and in vitro bioactivity of sol‐gel‐derived SiO2–CaO–P2O5–MgO bioglass10.1016/j.msec.2008.07.004 ↗
53. Synthesis and characterization of hierarchically macroporous and mesoporous CaO–MO–SiO2–P2O5 (M=Mg, Zn, Sr) bioactive glass scaffolds10.1016/j.actbio.2011.06.029 ↗
54. Sol gel derived SiO2‐CaO‐MgO‐P2O5 bioglass system—Preparation andin vitro characterization10.1002/jbm.b.30827 ↗
55. Risk factors associated with deep surgical site infections after primary total knee arthroplasty: an analysis of 56,216 knees10.2106/JBJS.L.00211 ↗
56. Comparison of osseointegration on various implant surfaces after bacterial contamination and cleaning: a rabbit study10.11607/jomi.2436 ↗
57. Substituted hydroxyapatites with antibacterial properties10.1155/2014/178123 ↗
58. Synthesis and evaluation of mechanical and biological properties of scaffold prepared from Ti and Mg with different volume percent10.1080/15533174.2013.862667 ↗
59. Solutions able to reproducein vivo surface‐structure changes in bioactive glass‐ceramic A‐W310.1002/jbm.820240607 ↗
60. Osteoblast‐like cell (MC3T3‐E1) proliferation on bioerodible polymers: an approach towards the development of a bone‐bioerodible polymer composite material10.1016/0142-9612(93)90116-J ↗
61. In vitro mineralization of osteoblastic cells derived from human bone10.1016/0169-6009(90)90109-S ↗
62. Functional gap junctions between osteocytic and osteoblastic cells10.1359/jbmr.2000.15.2.209 ↗
63. Antibacterial effect of bioactive glasses on clinically important anaerobic bacteria in vitro
64. Antibacterial effects and dissolution behavior of six bioactive glasses10.1002/jbm.a.32564 ↗
65. Study on antibacterial effect of 45S5 Bioglass®
66. Gel‐derived SiO2–CaO–Na2O–P2O5 bioactive powders: synthesis and in vitro bioactivity10.1016/j.msec.2011.02.018 ↗
67. Hydrothermal synthesis of hollow hydroxyapatite microspheres with nano‐structured surface assisted by inositol hexakisphosphate10.1016/j.ceramint.2014.02.057 ↗
68. Investigation of the physico‐chemical reactivity of a mesoporous bioactive SiO2–CaO–P2O5 glass in simulated body fluid10.1016/j.jnoncrysol.2010.04.040 ↗
69. Synthesis and characterization of bioactive glass/forsterite nanocomposites for bone implants
70. Bioactivity and mechanical behaviour of cobalt oxide‐doped bioactive glass10.1007/s12034-015-0936-6 ↗
71. Effects of materials parameters on mineralization and degradation of sol‐gel bioactive glasses with 3D‐ordered macroporous structures10.1002/jbm.a.10093 ↗
72. A comparative study on the in vitro formation of hydroxyapatite, cytotoxicity and antibacterial activity of 58S bioactive glass substituted by Li and Sr10.1016/j.msec.2018.05.058 ↗
73. Influence of strontium ion on the crystallization of hydroxyapatite from aqueous solution10.1021/j150616a022 ↗
74. Effect of MgO contents on the mechanical properties and biological performances of bioceramics in the MgO–CaO–SiO2 system10.1007/s10856-010-4025-5 ↗
75. Bioactive glass fillers reduce bacterial penetration into marginal gaps for composite restorations10.1016/j.dental.2015.10.007 ↗
76. Strain specificity in antimicrobial activity of silver and copper nanoparticles10.1016/j.actbio.2007.11.006 ↗
77. In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus10.1016/j.actbio.2009.10.007 ↗
78. Antibacterial activity of particulate Bioglass® against supra‐ and subgingival bacteria10.1016/S0142-9612(00)00330-6 ↗
79. Hench LL · 199310.1142/2028 ↗
80. New trends in bioactive scaffolds: the importance of nanostructure10.1016/j.jeurceramsoc.2008.08.003 ↗
81. Copper‐containing mesoporous bioactive glass promotes angiogenesis in an in vivo zebrafish model10.1016/j.actbio.2017.12.032 ↗
82. Effect of copper‐doped silicate 13–93 bioactive glass scaffolds on the response of MC3T3‐E1 cells in vitro and on bone regeneration and angiogenesis in rat calvarial defects in vivo10.1016/j.msec.2016.05.073 ↗
83. Hünlich base derivatives as photo‐responsive Λ‐shaped hinges10.1039/C6QO00653A ↗
84. Grafting amino drugs to poly(styrene‐alt‐maleic Anhydride) as a potential method for drug release