Biogenic hydroxyapatite from camel and bovine femur bones: physicochemical characterization and biomedical potential

1. Akram, M., Ahmed, R., Shakir, I., Ibrahim, W.A.W., Hussain, R.: Extracting hydroxyapatite and its precursors from natural resources. J. Mater. Sci. 49, 1461–1475 (2014). https://doi.org/10.1007/s10853-013-7864-x10.1007/s10853-013-7864-x ↗

2. Ghasemi, E., Sillanpää, M.: Magnetic hydroxyapatite nanoparticles: An efficient adsorbent for the separation and removal of nitrate and nitrite ions from environmental samples: Other techniques. J. Sep. Sci. 38, 164–169 (2015). https://doi.org/10.1002/jssc.20140092810.1002/jssc.201400928 ↗

3. Hammood, A.S., Hassan, S.S., Alkhafagy, M.T.: Comparison of natural and Nano-synthetically-Produced hydroxyapatite powder. JOM. 71, 272–278 (2019). https://doi.org/10.1007/s11837-018-3185-510.1007/s11837-018-3185-5 ↗

4. Ruksudjarit, A., Pengpat, K., Rujijanagul, G., Tunkasiri, T.: Synthesis and characterization of nanocrystalline hydroxyapatite from natural bovine bone. Curr. Appl. Phys. 8, 270–272 (2008). https://doi.org/10.1016/j.cap.2007.10.07610.1016/j.cap.2007.10.076 ↗

5. Toque, J.A., Herliansyah, M., Hamdi, M., Ide-Ektessabi, A., Wildan, M.: The effect of sample Preparation and calcination temperature on the production of hydroxyapatite from bovine bone powders. IFMBE Proc. 15, 187–192 (2007). https://doi.org/10.1007/978-3-540-68017-8_3910.1007/978-3-540-68017-8_39 ↗

6. Amna, T.: Valorization of bone waste of Saudi Arabia by synthesizing hydroxyapatite. Appl. Biochem. Biotechnol. 186, 779–788 (2018). https://doi.org/10.1007/s12010-018-2768-510.1007/s12010-018-2768-5 ↗

7. Hartati, Y.W., Irkham, I., Zulqaidah, S., Syafira, R.S., Kurnia, I., Noviyani, A.R., Topkaya, S.N.: Recent advances in hydroxyapatite-based electrochemical biosensors: Applications and future perspectives. Sens. Bio-Sensing Res. 38, 100542 (2022). https://doi.org/10.1016/j.sbsr.2022.10054210.1016/j.sbsr.2022.100542 ↗

8. Adamu, D.B., Zereffa, E.A., Segne, T.A., Razali, M.H., Lemu, B.R.: Synthesis and characterization of bismuth-doped hydroxyapatite nanorods for fluoride removal. Environ. Adv. 12, 100360 (2023). https://doi.org/10.1016/j.envadv.2023.10036010.1016/j.envadv.2023.100360 ↗

9. Ghedjemis, A., Benouadah, A., Fenineche, N., Ayeche, R., Hatim, Z., Drouiche, N., Lounici, H.: Preparation of hydroxyapatite from dromedary bone by heat treatment. Int. J. Environ. Res. 13, 547–555 (2019). https://doi.org/10.1007/s41742-019-00198-510.1007/s41742-019-00198-5 ↗

10. Nayak, A., Bhushan, B.: Hydroxyapatite as an advanced adsorbent for removal of heavy metal ions from water: Focus on its applications and limitations. Mater. Today: Proc. 46, 11029–11034 (2021). https://doi.org/10.1016/j.matpr.2021.02.14910.1016/j.matpr.2021.02.149 ↗

11. Ramdani, A., Kadecheun, A., Adjdirb, M., Taleb, Z., Ikhouun, D., Taleb, S., Deratani, A.: Lead and cadmium removal by adsorption process using hydroxyapatite porous materials. Water Pract. Technol. 15, 130–141 (2020). https://doi.org/10.2166/wpt.2020.00310.2166/wpt.2020.003 ↗

12. Mobasherpour, I., Salahi, E., Pazouki, M.: Comparative of the removal of Pb²⁺, Cd²⁺ and Ni²⁺ by nano crystallite hydroxyapatite from aqueous solutions: Adsorption isotherm study. Arab. J. Chem. 5, 439–446 (2012). https://doi.org/10.1016/j.arabjc.2010.12.02210.1016/j.arabjc.2010.12.022 ↗

13. Dang, N.-T.N., Le, H.-P., Toi, V., Van, Nguyen, T.-H.: A Comparative Study on Hydroxyapatite Derived from Bovine Bones and Synthetic Sources. In: 7th International Conference on the Development of Biomedical Engineering in Vietnam (BME7), IFMBE Proc., vol. 69, pp. 175–178. Springer, Singapore (2020). https://doi.org/10.1007/978-981-13-5859-3_2910.1007/978-981-13-5859-3_29 ↗

14. Khamkongkaeo, A., Boonchuduang, T., Klysubun, W., Amonpattaratkit, P., Chunateun, H.T., Tuchinda, N., Pimsawat, A., Daengsakul, S., Suksangrat, P., Sailuam, W., Vongpramate, D., Bootchanont, A., Lohwongwatana, B.: Sintering behavior and mechanical properties of hydroxyapatite ceramics prepared from nile tilapia (Oreochromis niloticus) bone and commercial powder for biomedical applications. Ceram. Int. 47, 34575–34584 (2021). https://doi.org/10.1016/j.ceramint.2021.08.37210.1016/j.ceramint.2021.08.372 ↗

15. Esmaeilkhanian, A., Sharifianjazi, F., Abouchenari, A., Rouhani, A., Parvin, N., Irani, M.: Synthesis and characterization of natural nano-hydroxyapatite derived from Turkey femur-bone waste. Appl. Biochem. Biotechnol. 189, 919–932 (2019). https://doi.org/10.1007/s12010-019-03046-610.1007/s12010-019-03046-6 ↗

16. Pu’ad, M., Koshy, N.A.S., Abdullah, P., Idris, H.Z., Lee, M.I., T.C.: Syntheses of hydroxyapatite from natural sources. Heliyon. 5, e01588 (2019). https://doi.org/10.1016/j.heliyon.2019.e0158810.1016/j.heliyon.2019.e01588 ↗

17. Islam, M., Mishra, P.C., Patel, R.: Physicochemical characterization of hydroxyapatite and its application towards removal of nitrate from water. J. Environ. Manage. 91, 1883–1891 (2010). https://doi.org/10.1016/j.jenvman.2010.04.01310.1016/j.jenvman.2010.04.013 ↗

18. Alkan, B., Durucan, C.: Complete chemical and structural characterization of selenium-incorporated hydroxyapatite. J. Mater. Sci. Mater. Med. 33, 5 (2022). https://doi.org/10.1007/s10856-021-06631-610.1007/s10856-021-06631-6 ↗

19. Khoo, W., Nor, F.M., Ardhyananta, H., Kurniawan, D.: Preparation of natural hydroxyapatite from bovine femur bones using calcination at various temperatures. Procedia Manuf. 2, 196–201 (2015). https://doi.org/10.1016/j.promfg.2015.07.03410.1016/j.promfg.2015.07.034 ↗

20. Odusote, J.K., Danyuo, Y., Baruwa, A.D., Azeez, A.A.: Synthesis and characterization of hydroxyapatite from bovine bone for production of dental implants. J. Appl. Biomater. Funct. Mater. 17, 228080001983682 (2019). https://doi.org/10.1177/228080001983682910.1177/2280800019836829 ↗

21. Jouda, N.S., Essa, A.F.: Preparation and study of the structural, physical and mechanical properties of hydroxyapatite nanocomposite. Mater. Today Proc. 47, 5999–6005 (2021). https://doi.org/10.1016/j.matpr.2021.04.55010.1016/j.matpr.2021.04.550 ↗

22. Londoño-Restrepo, S.M., Millán-Malo, B.M., del Real-López, A., Rodriguez-García, M.E.: In situ study of hydroxyapatite from cattle during a controlled calcination process using HT-XRD. Mater. Sci. Eng. C. 105, 110020 (2019). https://doi.org/10.1016/j.msec.2019.11002010.1016/j.msec.2019.110020 ↗

23. Jamarun, N., Amelia, D., Rahmayeni, U., Septiani, V., Sisca, V.: The effect of temperature on the synthesis and characterization of hydroxyapatite-polyethylene glycol composites by in-situ process. Hybrid. Adv. 2, 100031 (2023). https://doi.org/10.1016/j.hybadv.2023.10003110.1016/j.hybadv.2023.100031 ↗

24. Avram, A., Frentiu, T., Horovitz, O., Mocanu, A., Goga, F., Tomoaia Cotisel, M.: Hydroxyapatite for removal of heavy metals from wastewater. Stud. Univ. Babes-Bolyai Chem. 62, 93–104 (2017). https://doi.org/10.24193/subbchem.2017.4.0810.24193/subbchem.2017.4.08 ↗

25. Huang, H., et al.: 3D-printed tri-element-doped hydroxyapatite/polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration. Bioact Mater. 31, 18–37 (2024). https://doi.org/10.1016/j.bioactmat.2023.07.00410.1016/j.bioactmat.2023.07.004 ↗

26. Ivankovic, T., Turk, H., Hrenovic, J., Schauperl, Z., Ivankovic, M., Ressler, A.: Antibacterial activity of silver doped hydroxyapatite toward multidrug-resistant clinical isolates of acinetobacter baumannii. J. Hazard. Mater. 458, 131867 (2023). https://doi.org/10.1016/j.jhazmat.2023.13186710.1016/j.jhazmat.2023.131867 ↗

27. Algahtani, F.D., et al.: Development of ternary nanocomposites based on hydroxyapatite/silver oxide for biomedical applications: morphology, structure and antibacterial activity. J. Mater. Res. Technol. 20, 60–72 (2022). https://doi.org/10.1016/j.jmrt.2022.07.01510.1016/j.jmrt.2022.07.015 ↗

28. Dorozhkin, S.V.: Bioceramics of calcium orthophosphates. Biomaterials. 31, 1465–1485 (2010). https://doi.org/10.1016/j.biomaterials.2009.11.05010.1016/j.biomaterials.2009.11.050 ↗

29. Li, B., Zhang, Y., Wang, Y., Zhao, D.: Advances in therapeutic applications of biogenic hydroxyapatite nanoparticles. J. Nanobiotechnol. 17, 1–19 (2019). https://doi.org/10.1186/s12951-019-0475-210.1186/s12951-019-0475-2 ↗

30. Oliveira, H.C., Oliveira, M.L., Rangel, É.C., Passos, G.A.: Anti-inflammatory effect of hydroxyapatite nanoparticles in animal models. Biomed. Pharmacother. 146, 112578 (2022). https://doi.org/10.1016/j.biopha.2022.11257810.1016/j.biopha.2022.112578 ↗

31. Rehman, I., Bonfield, W.: Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy. J. Mater. Sci. Mater. Med. 8, 1–4 (2014). https://doi.org/10.1007/BF0291418010.1007/BF02914180 ↗

32. Sadat-Shojai, M., Khorasani, M.T., Dinpanah-Khoshdargi, E., Jamshidi, A.: Synthesis methods for nanosized hydroxyapatite with diverse structures. Acta Biomater. 9, 7591–7621 (2013). https://doi.org/10.1016/j.actbio.2013.04.01210.1016/j.actbio.2013.04.012 ↗

33. Sharma, S., Saikia, A., Deb, P.: Protein stabilization by nanomaterials: Mechanism and biomedical applications. ACS Biomater. Sci. Eng. 6, 692–712 (2020). https://doi.org/10.1021/acsbiomaterials.9b0143010.1021/acsbiomaterials.9b01430 ↗

34. Ciobanu, C.S., Iconaru, S.L., Le Coustumer, P., Constantin, L.V., Predoi, D.: Antibacterial activity of silver-doped hydroxyapatite nanoparticles against Gram-positive and Gram-negative bacteria. Nanoscale Res. Lett. 7, 324 (2012). http://www.nanoscalereslett.com/content/7/1/32410.1186/1556-276X-7-324 ↗

35. Habib, M.L., Uddin, M.N., Disha, S.A., Ahmed, S., Hossain, M.S.: Enhancement of antimicrobial properties by metals doping in nano-crystalline hydroxyapatite for efficient biomedical applications. Heliyon. 10, e23845 (2024). https://www.cell.com/heliyon/fulltext/S2405-8440(24)04269-610.1016/j.heliyon.2023.e23845 ↗

36. Nathanael, A.J., Lee, J.H., Mangalaraj, D., Hong, S.I., Rhee, Y.H.: Multifunctional properties of hydroxyapatite/titania bio-nano-composites: Bioactivity and antimicrobial studies. Powder Technol. 228, 410–415 (2012). https://doi.org/10.1016/j.powtec.2012.05.0510.1016/j.powtec.2012.05.05 ↗

37. Abdelmoneim, D., Porter, G.C., Coates, D.E., Duncan, W.J., Waddell, J.N., Hammer, N., Li, K.C.: The effect of low-processing temperature on the physicochemical and mechanical properties of bovine hydroxyapatite bone substitutes. Materials. 15, 2798 (2022). https://doi.org/10.3390/ma1508279810.3390/ma15082798 ↗

38. Bouyegh, S., Tlili, S., Labiod, K., Hassani, M., Grimet, M., Bensalem, O.: Preparation and characteristics of synthesized hydroxyapatite from bovine bones and by co-precipitation method. In: Proc. Int. Conf. Ind. Eng. Oper. Manag., Monterrey, Mexico, 3–5 Nov (2021). https://doi.org/10.46254/NA06.202110.46254/NA06.2021 ↗

39. Said, T.O., Awwad, N.S., Amri, E.: Sorption of three PAHs profile groups using Eco-friendly and inexpensive hydroxyapatite extracted from camel bone. Waste Manag Bull. 2, 1–10 (2024). https://doi.org/10.1016/j.wmb.2024.06.00110.1016/j.wmb.2024.06.001 ↗

40. Bouyer, E., Gitzhofer, F., Boulos, M.I.: Morphological study of hydroxyapatite nanocrystal suspension. J. Mater. Sci. Mater. Med. 11, 523–531 (2000). https://doi.org/10.1023/A:100891811015610.1023/A:1008918110156 ↗

41. El-Zahhar, A.A., Awwad, N.S.: Removal of methylene blue dyes from aqueous system using composite polymeric-apatite resins. J. Environ. Chem. Eng. 4, 633–638 (2016). https://doi.org/10.1016/j.jece.2015.12.01410.1016/j.jece.2015.12.014 ↗

42. European Commission Scientific Committee on Consumer Safety: Opinion on Hydroxyapatite (nano), SCCS/1624/20-30-31 March 2021: (2021)

43. Ferraz, M.P., Monteiro, F.J., Manuel, C.M.: Hydroxyapatite nanoparticles: A review of Preparation methodologies. J. Appl. Biomater. Biomech. 2, 74–80 (2004). https://doi.org/10.1177/22808000040020020210.1177/228080000400200202 ↗

44. Gupta, H.: Anthracene removal from water onto activated carbon derived from vehicular tire. Sep. Sci. Technol. 53, 613–625 (2018). https://doi.org/10.1080/01496395.2017.140503810.1080/01496395.2017.1405038 ↗

45. Gupta, H., Gupta, B.: Adsorption of polycyclic aromatic hydrocarbons on banana Peel activated carbon. Desalin. Water Treat. 57, 9498–9509 (2016). https://doi.org/10.1080/19443994.2015.102900710.1080/19443994.2015.1029007 ↗

46. Haberko, K., Buckoa, M.M., Mozgawaa, W., Pydaa, A., Brzezi´nska-Miecznika, J., Carpentier, J.: Behaviour of bone origin hydroxyapatite at elevated temperatures and in O₂ and CO₂ atmospheres. Ceram. Int. 35, 2537–2540 (2009). https://doi.org/10.1016/j.ceramint.2009.02.00810.1016/j.ceramint.2009.02.008 ↗

47. Hendi, A.A.: Hydroxyapatite based nanocomposite ceramics. J. Alloy Comp. 712, 147–151 (2017). https://doi.org/10.1016/j.jallcom.2017.04.02110.1016/j.jallcom.2017.04.021 ↗

48. Koliyabandara, P.A., Hettithanthri, O., Rathnayake, A., Rajapaksha, A.U., Nanayakkara, N., Vithanage, M.: Integrated Environmental Technologies for Wastewater Treatment and Sustainable Development. In: Kumar, V., Kumar, M. (eds.) Hydroxyapatite for environmental remediation of water/wastewater, pp. 167–191. Elsevier (2022). https://doi.org/10.1016/B978-0-323-91180-1.00004-110.1016/B978-0-323-91180-1.00004-1 ↗

49. O’Hare, P., Meenan, B.J., Burke, G.A., Byrne, G., Dowling, D., Hunt, J.A.: Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique. Biomaterials. 31, 515–522 (2010). https://doi.org/10.1016/j.biomaterials.2009.09.06710.1016/j.biomaterials.2009.09.067 ↗

50. Jin, P., Liu, L., Cheng, L., et al.: Calcium-to-phosphorus releasing ratio affects osteoinductivity and osteoconductivity of calcium phosphate bioceramics in bone tissue engineering. BioMedical Eng. (2023). https://doi.org/10.1186/s12938-023-01067-110.1186/s12938-023-01067-1 ↗

51. Sadeghian Dehkord, E., De Carvalho, B., Ernst, M., Albert, A., Lambert, F., Geris, L.: Influence of physicochemical characteristics of calcium phosphate-based biomaterials in cranio-maxillofacial bone regeneration: A systematic literature review and meta-analysis of preclinical models. Mater. Today Bio. 26, 101100 (2024). https://doi.org/10.1016/j.mtbio.2024.10110010.1016/j.mtbio.2024.101100 ↗

52. Gowthaman, S., Yamamoto, M., Chen, M., Nakashima, K., Kawasaki, S.: Baseline investigation on enzyme induced calcium phosphate precipitation for solidification of sand. Front. Built Environ. 9, 1307650 (2023). https://doi.org/10.3389/fbuil.2023.130765010.3389/fbuil.2023.1307650 ↗

53. Avramenko, M., Nakashima, K., Kawasaki, S.: State-of-the-Art review on engineering uses of calcium phosphate compounds: An Eco-Friendly approach for soil improvement. Mater. 15, 6878 (2022). https://doi.org/10.3390/ma1519687810.3390/ma15196878 ↗

54. Salama, A.: Cellulose/calcium phosphate hybrids: New materials for biomedical and environmental applications. Int. J. Biol. Macromol. (2019). https://doi.org/10.1016/j.ijbiomac.2019.01.13010.1016/j.ijbiomac.2019.01.130 ↗

55. Hosseinzadeh, E., Davarpanah, M., Hassanzadeh Nemati, N., Tavakoli, S.A.: Fabrication of a hard-tissue replacement using natural hydroxyapatite derived from bovine bones by thermal decomposition method. Int. J. Organ. Transpl. Med. 5(1), 23–31 (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC4089331

56. Nirmala, R., Sheikh, A., Kanjwal, M., Lee, J., Parc, S.J., Navamathavan, R., Kim, H.Y.: Synthesis and characterization of bovine femur bone hydroxyapatite containing silver nanoparticles for the biomedical applications. J. Nanopart. Res. 13, 1917–1927 (2011). https://doi.org/10.1007/s11051-010-9944-z10.1007/s11051-010-9944-z ↗

57. Jaber, H.L., Hammood, A.S., Parvin, N.: Synthesis and characterization of hydroxyapatite powder from natural Camelus bone. J. Aust Ceram. Soc. 54, 1–10 (2018). https://doi.org/10.1007/s41779-017-0120-010.1007/s41779-017-0120-0 ↗

58. Nassar, A.M., Arafa, W.A.A., Ashammari, K., Moustafa, S.M.N., Alsirhani, A.M., Hasaneen, M.F.: A new green catalyst and antimicrobial agent derived from eco-friendly products of camel bones: Synthesis and physicochemical characterization. Biomass Conv Biorefinery. 15, 13589–13607 (2025). https://doi.org/10.1007/s13399-024-06234-510.1007/s13399-024-06234-5 ↗

59. Alanazi, A.H., Arafa, W.A.A., Moustafa, S.M.N., Alsohaimi, I.H., Elnasr, T.A.S., Halawani, R.F., Al Zbedy, A.S., Nassar, A.M.: Green extraction of biomass from waste goat bones for applications in catalysis, wastewater treatment, and water disinfection. J. Hazard. Mater. Adv. 18, 100645 (2025). https://doi.org/10.1016/j.hazadv.2025.10064510.1016/j.hazadv.2025.100645 ↗

60. Mishra, P., Singh, U., Pandey, C.M., Mishra, P., Pandey, G.: Application of student’s t-test, analysis of variance, and covariance. Ann. Cardiac Anaesth. 23, 86–89 (2020). https://doi.org/10.4103/aca.ACA_94_1910.4103/aca.ACA_94_19 ↗

61. Lin, M.C., Boehnke, T., Lee, J.A., Torreggiani, E., Yang, L.: Fracture characteristics of human cortical bone influenced by advanced glycation end-products. JBMR Plus. 9, e151 (2025). https://doi.org/10.1093/jbmrplus/ziae15110.1093/jbmrplus/ziae151 ↗

62. Galotta, A., Rubenis, K., Locs, J., Bitenieks, J., Salma, I.: Elastic modulus and hardness of cortical and trabecular bovine bone measured by nanoindentation. J. Mech. Behav. Biomed. Mater. 145, 106035 (2023). https://doi.org/10.1016/j.jmbbm.2023.10603510.1016/j.jmbbm.2023.106035 ↗

63. Costa, G.A., et al.: Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis. Acad. Bras. Ciênc. 90, 73–84 (2018). https://doi.org/10.1590/0001-376520182016013110.1590/0001-3765201820160131 ↗

64. Madhuranga, H.D.T., Samarakoon, D.N.A.W.: In vitro Anti-inflammatory egg albumin denaturation assay: An enhanced approach. J. Nat. Ayurvedic Med. 7, 1–6 (2023). https://doi.org/10.23880/jonam-1600041110.23880/jonam-16000411 ↗

65. Pushpika, K.G.C.M., et al.: Evaluation of anti-inflammatory activity of Sri Lankan traditional medicine pill by in vitro protein denaturation method. Asian Plant. Res. J. 11, 1–10 (2023). https://doi.org/10.9734/aprj/2023/v11i322710.9734/aprj/2023/v11i3227 ↗

66. Gunathilake, K.D.P.P., Ranaweera, K.K.D.S., Rupasinghe, H.P.V.: In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines. 6, 107 (2018). https://doi.org/10.3390/biomedicines604010710.3390/biomedicines6040107 ↗

67. Yesmin, S., Paul, A., Rahman, A.B.M.A., Biswas, A.K., Sarker, S., Khan, M.A.I., Hossain, M.T.: Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba). Clin. Phytoscience. 6, 59 (2020). https://doi.org/10.1186/s40816-020-00207-710.1186/s40816-020-00207-7 ↗

68. Shrivastava, S., Dash, D., Tripathi, P.: Cytotoxicity and antibacterial activity of silver nanoparticle embedded hydroxyapatite composites for orthopedic applications. Mater. Sci. Eng. C. 69, 1153–1162 (2016). https://doi.org/10.1016/j.msec.2016.08.03310.1016/j.msec.2016.08.033 ↗

69. Durán, N., Silveira, C.P., Durán, M., Martinez, D.S.T.: Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity. J. Biomed. Mater. Res. B Appl. Biomater. 106(3), 1285–1299 (2018). https://doi.org/10.1002/jbm.b.3386010.1002/jbm.b.33860 ↗

70. Pal, A., Paul, S., Choudhury, S., Sarkar, S.: Cytotoxic and antibacterial evaluation of zinc-doped hydroxyapatite for biomedical applications. Mater. Sci. Eng. C. 44, 193–202 (2014). https://doi.org/10.1016/j.msec.2014.08.03210.1016/j.msec.2014.08.032 ↗

71. Mirzaali, M.J., Schwiedrzik, J.J., Thaiwichai, S., Best, J.P., Michler, J., Zysset, P.K., Wolfram, U.: Mechanical properties of cortical bone and their relationships with age, gender, composition and microindentation properties in the elderly. Bone. 93, 196–211 (2016). https://doi.org/10.1016/j.bone.2015.11.01810.1016/j.bone.2015.11.018 ↗

72. Yin, B., Guo, J.-L., Wang, J.-Z., Li, S., Liu, Y.-K., Zhang, Y.-Z.: Bone material properties of human phalanges using Vickers indentation. Orthop. Surg. 11, 487–492 (2019). https://doi.org/10.1111/os.1245510.1111/os.12455 ↗

73. Ibrahim, A., Magliulo, N., Groben, J., Padilla, A., Akbik, F., Abdel Hamid, Z.: Hardness, an important indicator of bone Quality, and the role of collagen in bone hardness. J. Funct. Biomater. 11, 85 (2020). https://doi.org/10.3390/jfb1104008510.3390/jfb11040085 ↗

74. Weissgerber, T.L., Garcia-Valencia, O., Garovic, V.D., Milic, N.M., Winham, S.J.: Why we need to report more than ‘data were analyzed by t-tests or ANOVA’. eLife. 7, e36163 (2018). https://doi.org/10.7554/eLife.3616310.7554/eLife.36163 ↗

75. Dada, A.A., Ayodele, S.O., Ogunwolu, S.O., Oyeyinka, S.A.: Protein hydrolysates from Artocarpus altilis seeds exhibit in vitro antioxidant and anti-inflammatory activities. Sci. Rep. 13, 1394 (2023). https://doi.org/10.1038/s41598-023-28684-z10.1038/s41598-023-28684-z ↗

76. JCHR: Anti-inflammatory properties of terbium doped hydroxyapatite. J. Chem. Pharm. Res. 15, 70–78 (2023). https://doi.org/10.22270/jddt.v15i3.820210.22270/jddt.v15i3.8202 ↗

77. Li, S., et al.: Osteogenic and anti-inflammatory effects of an injectable hydrogel scaffold with nano-hydroxyapatite for bone regeneration. Front. Bioeng. Biotechnol. 11, 1142839 (2023). https://doi.org/10.3389/fbioe.2023.114283910.3389/fbioe.2023.1142839 ↗