Platelet-rich plasma as an ideal biomaterial for improving pregnancy of infertility mice

Uterine implantation failure can be caused by uterine pathologies, including endometrial thinning or adhesions. This investigation aims to evaluate the effect of platelet-rich plasma (PRP) on endometrial regeneration. In this study, a double-dose non-activated PRP therapy was administered by repeatedly infusing a similar volume of non-activated PRP 24 h after the first dose in the in vitro damaged endometrial tissue models and in vivo injured uterine models. Histology, implantation, and pregnancy assessments of endometrial structure and function were performed. We discovered that in the in vitro models, the treated endometrial tissues appeared to have endometrial epithelium, uterine glands, pinopodes, and embryos that were able to implant on the endometrium. The rate of implanted embryos in the treated models was 18.89% ± 0.02 higher than those in the non-treated group with no embryo implantation (P-value <0.05). Animal models also showed similar results. The uterine structure was restored, the number of glands (143.3 ± 9.815) and the thickness (rate of endometrial area (EA)/total horn area (THA) was 0.4013 ± 0.0344) were increased in the treated models (P-value <0.05). The number of pregnancies (implantation sites) in the treated mouse models was 10.33 ± 1.53. In conclusion, double-dose non-activated PRP could effectively promote endometrial regeneration.