Novel Hydroxyapatite Whiskers Modified by Silver Ion and Nano Zinc Oxide Used for Bone Defect Repairment
Source abstract
Abstract
The hydroxyapatite (HA) is widely used as bone tissue repair material. The improvement of the antibacterial performance is an aroused general interest. In the present study, the silver ion and nano-zinc oxide modified hydroxyapatite whiskers (HAw) were successfully prepared. The microstructure and the composition of the modified HAw were analyzed by Field Emission Scanning Electron Microscopy (FESEM) and X-ray diffractometer (XRD). SEM analysis showed that the length of the whiskers was 70–190 μm, and the aspect ratio was 10–60. With the increase of Ag+ content, the length and aspect ratio of the whiskers gradually decreased and incomplete spherical hydroxyapatite appeared. FEEM analysis showed that nano-zinc oxide particles on ZnO/3Ag-HAw surface are evenly distributed; the average particle size is less than 30 nm. XRD analysis showed that after sol-gel and calcination treatment, the nano-zinc oxide phase appeared in the diffraction pattern of ZnO/Ag-HAw. TEM analysis showed that the interplanar spacing of 5Ag-HAw increased slightly. The CCK-8 and cells co-culture assays were used to assess the proliferation and differentiation of MC3T3-E1 cells, respectively. The antibacterial abilities of the modified HAw against E. coli (ATCC25922) and S. aureus (ATCC6538) were investigated. The cell cytotoxicity test showed that the cytotoxicity level was 0, and there was no cytotoxicity. Cell adhesion experiments showed that ZnO/3Ag-HAw has good cell compatibility and biological activity. The modified hawthorn has a bacteriostatic rate of more than 90% and has good bacteriostatic activity.
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Research context
OpenAlex topics: Bone Tissue Engineering Materials · Thallium and Germanium Studies · Orthopaedic implants and arthroplasty
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650000, China
School of Stomatology, Kunming Medical University, Kunming 650000, China
Xianning CSG Photoelectric Glass Co., Ltd., Xianning 437000, China
School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang 110002, China
Funding
Undergraduate Training Programs for Innovation and Entrepreneurship of Yunnan Province
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